Research Article
Year 2023,
Volume: 24 Issue: 2, 175 - 179, 30.06.2023
Abstract
References
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- 2. Popruk S, Adao DEV, Rivera WL. Epidemiology and subtype distribution of Blastocystis in humans: A review. Infect Genet Evol 2021; 95: 105085.
- 3. Parkar U, Traub RJ, Kumar S, Mungthin M, Vitali S, Leelayoova S, et al. Direct characterization of Blastocystis from faeces by PCR and evidence of zoonotic potential. Parasitology 2007; 134: 359-67.
- 4. Tan KS. New insights on classification, identification, and clinical relevance of Blastocystis spp. Clin Microbiol Rev 2008; 21: 639-65.
- 5. Skotarczak B. Genetic diversity and pathogenicity of Blastocystis. Ann Agric Environ Med 2018; 25: 411-6.
- 6. Scicluna SM, Tawari B, Clark CG. DNA barcoding of blastocystis. Protist 2006; 157: 77-85.
- 7. Roberts T, Stark D, Harkness J, Ellis J. Update on the pathogenic potential and treatment options for Blastocystis sp. Gut Pathog 2014; 6: 17.
- 8. Tan KS, Mirza H, Teo JD, Wu B, Macary PA. Current Views on the Clinical Relevance of Blastocystis spp. Curr Infect Dis Rep 2010; 12: 28-35.
- 9. Casqueiro J, Casqueiro J, Alves C. Infections in patients with diabetes mellitus: A review of pathogenesis. Indian J Endocrinol Metab 2012; 16 Suppl 1: S27-36.
- 10. Htun NSN, Odermatt P, Paboriboune P, Sayasone S, Vongsakid M, Phimolsarn-Nusith V, et al. Association between helminth infections and diabetes mellitus in adults from the Lao People’s Democratic Republic: a cross-sectional study. Infect Dis Poverty 2018; 7: 105.
- 11. Machado ER, Matos NO, Rezende SM, Carlos D, Silva TC, Rodrigues L, et al. Host-Parasite Interactions in Individuals with Type 1 and 2 Diabetes Result in Higher Frequency of Ascaris lumbricoides and Giardia lamblia in Type 2 Diabetic Individuals. J Diabetes Res 2018; 2018: 4238435.
- 12. Popruk N, Prasongwattana S, Mahittikorn A, Palasuwan A, Popruk S, Palasuwan D. Prevalence and Subtype Distribution of Blastocystis Infection in Patients with Diabetes Mellitus in Thailand. Int J Environ Res Public Health 2020; 17: 8877.
- 13. Poorkhosravani Z, Agholi M, Sharifi-Sarasiabi K, Heydari-Hengami M, Shamseddin J. Frequency of Intestinal Parasites in Patients with Diabetes Mellitus Compared with Healthy Controls in Fasa, Fars Province, Iran, 2018. Hormozgan Medical Journal 2019; 23: e91284.
- 14. Ibrahim SS, Ismail MAM, Shaker MA, Khalili DM, Raafat A. Blastocystıs hominis in diabetic and non-diabetic patients with irritable bowel syndrome in Beni-Suef city, Egypt. Journal of the Egyptian Society of Parasitology 2020; 50: 683-8.
- 15. Tangi FB, Fokam EB, Longdoh NA, Eteneneng EJ. Intestinal parasites in diabetes mellitus patients in the Limbe and Buea Municipalities, Cameroon. Diabetes Res Open J 2016; 2: 1-7.
- 16. Stensvold CR. Blastocystis: Genetic diversity and molecular methods for diagnosis and epidemiology. Trop Parasitol 2013; 3: 26-34.
- 17. Eida AM, Eida MM. Identification of Blastocystis hominis in Patients with Irritable Bowel Syndrome using Microscopy and Culture Compared to PCR. Parasitologist U J 2008.
- 18. Mohamed RT, El-Bali MA, Mohamed AA, Abdel-Fatah MA, El-Malky MA, Mowafy NM, et al. Subtyping of Blastocystis sp. isolated from symptomatic and asymptomatic individuals in Makkah, Saudi Arabia. Parasit Vectors 2017; 10: 174.
- 19. Dagci H, Kurt O, Demirel M, Mandiracioglu A, Aydemir S, Saz U, et al. Epidemiological and diagnostic features of blastocystis infection in symptomatic patients in izmir province, Turkey. Iran J Parasitol 2014; 9: 519-29.
- 20. Elghareeb AS, Younis MS, El Fakahany AF, Nagaty IM, Nagib MM. Laboratory diagnosis of Blastocystis spp. in diarrheic patients. Trop Parasitol 2015; 5: 36-41.
- 21. Dogan N, Aydin M, Tuzemen NU, Dinleyici EC, Oguz I, Dogruman-Al F. Subtype distribution of Blastocystis spp. isolated from children in Eskisehir, Turkey. Parasitol Int 2017; 66: 948-51.
Year 2023,
Volume: 24 Issue: 2, 175 - 179, 30.06.2023
Abstract
Objective: This study aimed to detect Blastocystis spp. To determine the incidence, genotypes, regulation of blood glucose level and its role in gastrointestinal symptoms.
Materials and Methods: The current study was conducted at Aydın Adnan Menderes University Medical Faculty Hospital, where stool samples of 100 patients diagnosed with type 2 diabetes mellitus (DM) and 100 healthy volunteers as a control group were examined. Stool samples were evaluated by direct microscopic examination and polymerase chain reaction (PCR). Statistical analysis of the data was performed using the SPSS 17.0 program, and a significance level of p<0.05 was determined.
Results: The presence of Blastocystis was investigated in a total of 200 samples. Of the cases, 81 (40.5%) were female and 119 (59.5%) were male. The mean age was 57.1±11.2. Most of the participants resided in the city center (52.5%). Eighteen samples that were Blastocystis positive by direct microscopy were also found to be positive by PCR. Additionally, two samples that were negative by direct microscopy in the control group were found to be positive by PCR. There was no significant difference between the groups in terms of Blastocystis positivity. The sensitivity and specificity of the direct microscopy method were calculated as 81.81% and 100%, respectively. Nine (45%) of the Blastocystis subtypes were ST3, seven (35%) were ST2, three (15%) were ST1, and one (5%) was ST6.
Conclusion: Blastocystis spp. is a pathogen that should be considered in patients with diabetes. Our study is one of the few studies investigating the prevalence and clinical features of Blastocystis spp. in patients with type 2 DM.
Keywords
References
- 1. Parija SC, Jeremiah S. Blastocystis: Taxonomy, biology and virulence. Trop Parasitol 2013; 3: 17-25.
- 2. Popruk S, Adao DEV, Rivera WL. Epidemiology and subtype distribution of Blastocystis in humans: A review. Infect Genet Evol 2021; 95: 105085.
- 3. Parkar U, Traub RJ, Kumar S, Mungthin M, Vitali S, Leelayoova S, et al. Direct characterization of Blastocystis from faeces by PCR and evidence of zoonotic potential. Parasitology 2007; 134: 359-67.
- 4. Tan KS. New insights on classification, identification, and clinical relevance of Blastocystis spp. Clin Microbiol Rev 2008; 21: 639-65.
- 5. Skotarczak B. Genetic diversity and pathogenicity of Blastocystis. Ann Agric Environ Med 2018; 25: 411-6.
- 6. Scicluna SM, Tawari B, Clark CG. DNA barcoding of blastocystis. Protist 2006; 157: 77-85.
- 7. Roberts T, Stark D, Harkness J, Ellis J. Update on the pathogenic potential and treatment options for Blastocystis sp. Gut Pathog 2014; 6: 17.
- 8. Tan KS, Mirza H, Teo JD, Wu B, Macary PA. Current Views on the Clinical Relevance of Blastocystis spp. Curr Infect Dis Rep 2010; 12: 28-35.
- 9. Casqueiro J, Casqueiro J, Alves C. Infections in patients with diabetes mellitus: A review of pathogenesis. Indian J Endocrinol Metab 2012; 16 Suppl 1: S27-36.
- 10. Htun NSN, Odermatt P, Paboriboune P, Sayasone S, Vongsakid M, Phimolsarn-Nusith V, et al. Association between helminth infections and diabetes mellitus in adults from the Lao People’s Democratic Republic: a cross-sectional study. Infect Dis Poverty 2018; 7: 105.
- 11. Machado ER, Matos NO, Rezende SM, Carlos D, Silva TC, Rodrigues L, et al. Host-Parasite Interactions in Individuals with Type 1 and 2 Diabetes Result in Higher Frequency of Ascaris lumbricoides and Giardia lamblia in Type 2 Diabetic Individuals. J Diabetes Res 2018; 2018: 4238435.
- 12. Popruk N, Prasongwattana S, Mahittikorn A, Palasuwan A, Popruk S, Palasuwan D. Prevalence and Subtype Distribution of Blastocystis Infection in Patients with Diabetes Mellitus in Thailand. Int J Environ Res Public Health 2020; 17: 8877.
- 13. Poorkhosravani Z, Agholi M, Sharifi-Sarasiabi K, Heydari-Hengami M, Shamseddin J. Frequency of Intestinal Parasites in Patients with Diabetes Mellitus Compared with Healthy Controls in Fasa, Fars Province, Iran, 2018. Hormozgan Medical Journal 2019; 23: e91284.
- 14. Ibrahim SS, Ismail MAM, Shaker MA, Khalili DM, Raafat A. Blastocystıs hominis in diabetic and non-diabetic patients with irritable bowel syndrome in Beni-Suef city, Egypt. Journal of the Egyptian Society of Parasitology 2020; 50: 683-8.
- 15. Tangi FB, Fokam EB, Longdoh NA, Eteneneng EJ. Intestinal parasites in diabetes mellitus patients in the Limbe and Buea Municipalities, Cameroon. Diabetes Res Open J 2016; 2: 1-7.
- 16. Stensvold CR. Blastocystis: Genetic diversity and molecular methods for diagnosis and epidemiology. Trop Parasitol 2013; 3: 26-34.
- 17. Eida AM, Eida MM. Identification of Blastocystis hominis in Patients with Irritable Bowel Syndrome using Microscopy and Culture Compared to PCR. Parasitologist U J 2008.
- 18. Mohamed RT, El-Bali MA, Mohamed AA, Abdel-Fatah MA, El-Malky MA, Mowafy NM, et al. Subtyping of Blastocystis sp. isolated from symptomatic and asymptomatic individuals in Makkah, Saudi Arabia. Parasit Vectors 2017; 10: 174.
- 19. Dagci H, Kurt O, Demirel M, Mandiracioglu A, Aydemir S, Saz U, et al. Epidemiological and diagnostic features of blastocystis infection in symptomatic patients in izmir province, Turkey. Iran J Parasitol 2014; 9: 519-29.
- 20. Elghareeb AS, Younis MS, El Fakahany AF, Nagaty IM, Nagib MM. Laboratory diagnosis of Blastocystis spp. in diarrheic patients. Trop Parasitol 2015; 5: 36-41.
- 21. Dogan N, Aydin M, Tuzemen NU, Dinleyici EC, Oguz I, Dogruman-Al F. Subtype distribution of Blastocystis spp. isolated from children in Eskisehir, Turkey. Parasitol Int 2017; 66: 948-51.
There are 21 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Infectious Diseases |
| Journal Section | Research Article |
| Authors | |
| Publication Date | June 30, 2023 |
| Published in Issue | Year 2023 Volume: 24 Issue: 2 |