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Development of Petal Culture Method in Kalanchoe (Kalanchoe blossfeldiana Poelnn.) and Investigation of its Potential Use in In Vitro Mutation Breeding Studies

Year 2025, Volume: 15 Issue: 1, 62 - 75, 26.06.2025

Kadriye Yaprak Kantoglu , Okan Sarıtoprak , Ebru Akyüz Çağdaş , Evrim Okutan , Hakan Aktaş , Şeküre Şebnem Ellialtıoğlu

https://doi.org/10.53518/mjavl.1552958

Abstract

Kalanchoe (Kalanchoe blossfeldiana Poelnn.), is an important potted indoor plant as well as an outdoor ornamental plant and cut flower in recent years. Studies are being carried out using different breeding methods in order to develop new varieties suitable for market needs. In vivo and in vitro mutation breeding studies are important for creating different genetic variations for this species, which is highly prone to mutation breeding. In in vitro mutation applications, vegetative propagation methods are of critical importance in mass propagation of mutant single individuals while preserving genetic stability. This study consists of two stages: First, development and optimization of petal culture method in kalanchoe to provide in vitro vegetative propagation of mutant individuals, and second, morphological observation of genetic stability in clones obtained by propagation via petal culture from M1V4 kalanchoe mutant single plants obtained by using ionizing radiation. In vitro petal culture conditions were determined for kalanchoe and it was determined that Murashige and Skoog (MS) nutrient medium containing 2.0 mg L-1 thidiazuron (TDZ), 0.5 mg L-1 1-naphthalenacetic acid (NAA), 30 g L-1 sucrose, 6 g L-1 agar and pH 5.7 provided the best regeneration. In addition, after in vitro physical mutagen application, flowers were observed in mutant individuals propagated up to M1V4 stage in laboratory conditions and transferred to external conditions. Petals from plants with 4 different mutant flowers selected from these were cultured and propagated in vitro. As control, petals from a commercial variety were used for micropropagation. The flowers of the clones obtained showed homogeneity depending on whether the mutant flowers used as starting material were homogeneous or chimeric in appearance. Following this study, in which the first findings on petal culture in kalanchoe were obtained, studies are continuing to develop it comprehensively.

Keywords

Kalanchoe in vitro mutation petal culture TDZ NAA gamma ray

Ethical Statement

Scientific rules, ethics and citation rules were followed during the all process of the study. No application that would harm nature or the environment was carried out during the research.

Supporting Institution

Has Biotech Research Development Agriculture Industry and Trade Inc. Co. and TENMAK

Project Number

-

Thanks

The research was supported by Has Biotech Research Development Agriculture Industry and Trade Inc. Co. and TENMAK provided technical consultancy. We thank them for their contributions.

References

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  • Bejaoui, R., Özdemir, G. E., & Ellialtıoğlu, Ş. Ş. (2023b). In vitro or ex vitro rooting and acclimatization of Kalanchoe blossfeldiana Poelln. shoots propagated by tissue culture technique. Turkish Journal of Agricultural and Natural Sciences (TTDB) 10(4): 843–853. https://doi.org/10.30910/turkjans.1288919
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Kalanşo’da (Kalanchoe blossfeldiana Poelnn.) Petal Kültürü Yönteminin Geliştirilmesi ve In Vitro Mutasyon Islahı Çalışmalarında Potansiyel Kullanımının Araştırılması

Year 2025, Volume: 15 Issue: 1, 62 - 75, 26.06.2025

Kadriye Yaprak Kantoglu , Okan Sarıtoprak , Ebru Akyüz Çağdaş , Evrim Okutan , Hakan Aktaş , Şeküre Şebnem Ellialtıoğlu

https://doi.org/10.53518/mjavl.1552958

Abstract

Kalanşo (Kalanchoe blossfeldiana Poelnn.), önemli bir saksılı iç mekân bitkisi olduğu gibi son yıllarda dış mekân süs bitkisi ve kesme çiçek olarak da öne çıkmaktadır. Pazar ihtiyaçlarına uygun yeni çeşitlerin geliştirilmesi amacıyla farklı ıslah yöntemleri kullanılarak çalışmalar yürütülmektedir. Mutasyon ıslahına oldukça yatkın olan bu tür için farklı genetik varyasyonlar oluşturmak amacıyla in vivo ve in vitro mutasyon ıslah çalışmaları önem taşımaktadır. In vitro mutasyon uygulamalarında vegetatif çoğaltım yöntemleri, mutant tek bireylerin genetik stabiliteyi koruyarak kitlesel çoğaltımının yapılmasında kritik öneme sahiptir. Bu çalışma iki aşamadan oluşmaktadır. Birincisi mutant bireylerin in vitro vegetatif çoğaltımını sağlamak üzere kalanşo türünde petal kültürü yönteminin geliştirilmesi ve optimizasyonu, ikincisi ise iyonize radyasyon kullanılarak elde edilmiş M1V4 kalanşo mutant tek bitkilerden petal kültürü yoluyla çoğaltım yaparak elde edilen klonlardaki genetik stabiliteyi morfolojik olarak gözlemlemek. Kalanşo için in vitro petal kültürü koşulları belirlenmiş ve 2.0 mg L-1 thidiazuron (TDZ), 0.5 mg L-1 1-naphthalenacetic asit (NAA), 30 g L-1 sukroz, 6 g L-1 agar ve ph 5.7 içeren Murashige ve Skoog (MS) besin ortamının en iyi rejenerasyonu sağladığı saptanmıştır. Ayrıca in vitro fiziksel mutagen uygulaması sonrasında laboratuvar koşullarında M1V4 aşamasına kadar çoğaltılan ve dış koşullara aktarılan mutant bireylerde çiçekler gözlenmiştir. Bunların arasından seçilen 4 farklı mutant çiçeklere sahip bitkilerden petaller in vitro kültüre alınmış ve çoğaltılmıştır. Kontrol olarak, mikroçoğaltım için ticari bir çeşidin taç yaprakları kullanıldı. Elde edilen klonların çiçekleri, başlangıç materyali olarak kullanılan mutant çiçeklerin homojen yapılı veya kimerik görünümlü olup olmadıklarına sağlı olarak homojenlik göstermiştir. Kalanşo’da petal kültürü konusundaki ilk bulguların elde edildiği bu çalışmanın ardından, kapsamlı bir şekilde geliştirilmesi konusunda çalışmalar devam etmektedir.

Keywords

Kalanşo in vitro mutasyon petal kültürü TDZ NAA gama ışını

Project Number

-

References

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  • Asoko, N., Ruamrungsri, S., Yoosumran, V., & Saetiew, K. (2020). Improvement of Dendranthemum grandiflora cv. Canter with colchicine in vitro. International Journal of Agricultural Technology 16(2): 237-246.
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  • Bejaoui, R. (2022). In Vitro Micropropagation of Kalanchoe (Kalanchoe blossfeldiana Poelln.). PhD Thesis. Ankara University, Institute of Natural and Applied Sciences, Department of Horticulture. 103p. Ankara.
  • Bejaoui, R., Özdemir, G. E., & Ellialtıoğlu, Ş. Ş. (2023b). In vitro or ex vitro rooting and acclimatization of Kalanchoe blossfeldiana Poelln. shoots propagated by tissue culture technique. Turkish Journal of Agricultural and Natural Sciences (TTDB) 10(4): 843–853. https://doi.org/10.30910/turkjans.1288919
  • Bejaoui, R., Gümüş, C., Sönmez, K., Kırbay, E., & Ellialtıoğlu, Ş. Ş. (2023a). The effects of different PGR contents on in vitro organogenesis and shoot proliferation in kalanchoe (Kalanchoe blossfeldiana Poelln.). In: 11th International Conference on Agriculture, Animal Sciences and Rural Development, 03-05 March 2023, Muş Alparslan University, Muş – Türkiye. Conference Proceedings Book (Eds: Karadağ, Y., Seydoşoğlu, S.). pp: 995-1010. ISBN:978-625-7720-91-5.
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  • Bhuiyan, M.S.U., Kim, T., In, J. G., Yang, D. C., & Choi, K. S. (2006). Plant regeneration from leaf explants of Kalanchoe daigremontiana Hamet & Perrier. Korean Journal of Medicinal Crop Science 14(5): 293-298
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  • Broertjes, C., & Leffring, L. (1972). Mutation breeding of Kalanchoë. Euphytica 21(3), 415-423.
  • Carra, A., Sajeva, M., Abbate, L., Siragusa, M., Sottile, F., & Carimi, F. (2012). In vitro plant regeneration of caper (Capparis spinosa L.) from floral explants and genetic stability of regenerants. Plant Cell Tissue and Organ Culture 109: 373–381. https://doi.org/10.1007/s11240-011-0102-9
  • CPVO varieties database.2017. Web page. http://cpvo.europa.eu. (Access date: 12.04.2017)
  • Datta, S. K. (2023). Technology package for induced mutagenesis. Journal of Biology and Nature, 15(1), 70-88.
  • Datta, S. K., Misra, P., & Mandal, A. K. A. (2005). In vitro mutagenesis – a quick method for establishment of solid mutant in Chrysanthemum. Current Science 88(1): 155-158.
  • Descoings, B. (2003). Kalanchoe. In: Eggli U, Hartmann HEK (eds) Illustrated Handbook of Succulent Plants. Crassulaceae. Springer Verlag, New York, pp 143–181.
  • Descoings, B. (2006). Le genre kalanchoe structure et définition. Journal de Botanique de la Société Botanique de France, 33, 3-28.
  • Ellialtıoğlu, Ş., & Yanmaz, R. (1994). Domates ve karnabaharda çift fazlı ortam ve aktif kömürün in vitro sürgün verim ve kalitesine etkisi. KÜKEM Dergisi 16 (1): 19-27.
  • Fransz, P. F., & Schel, J. H. N. (1994). Ultrastructural studies on callus development and somatic embryogenesis in Zea mays L. In: Maize (pp. 50-65). Berlin, Heidelberg: Springer Berlin Heidelberg.
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There are 59 citations in total.

Details

Primary Language English
Subjects Horticultural Production (Other)
Journal Section Research Article
Authors

Kadriye Yaprak Kantoglu 0000-0002-7247-9116

Okan Sarıtoprak 0009-0005-8106-8799

Ebru Akyüz Çağdaş 0000-0003-1630-807X

Evrim Okutan 0009-0000-7996-5371

Hakan Aktaş 0000-0001-8280-5758

Şeküre Şebnem Ellialtıoğlu 0000-0002-3851-466X

Project Number -
Publication Date June 26, 2025
Submission Date September 20, 2024
Acceptance Date December 15, 2024
Published in Issue Year 2025 Volume: 15 Issue: 1

Cite

APA Kantoglu, K. Y., Sarıtoprak, O., Akyüz Çağdaş, E., Okutan, E., et al. (2025). Development of Petal Culture Method in Kalanchoe (Kalanchoe blossfeldiana Poelnn.) and Investigation of its Potential Use in In Vitro Mutation Breeding Studies. Manas Journal of Agriculture Veterinary and Life Sciences, 15(1), 62-75. https://doi.org/10.53518/mjavl.1552958
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