Research Article
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Effects of Colchicine Applications on Embryo Yield and Spontaneous Chromosome Doubling in Pepper (Capsicum annuum L.) Anther Culture

Year 2025, Volume: 15 Issue: 1, 76 - 90, 26.06.2025

Beyza Nur Marangoz , Nuray Çömlekçioğlu , Ezgi Gürsoy , Büşra Yapici , Şeküre Şebnem Ellialtıoğlu

https://doi.org/10.53518/mjavl.1583740

Abstract

The haploidy technique starts with the production of haploid embryos and continues with the doubling of the chromosome set. Androgenic embryos can be either haploid (H) or spontan doubled haploid (SDH). Since haploid plants are sterile, anti-mitotic agents are applied at appropriate doses and durations to restore diploid status and fertility. Chromosome doubling can be induced in vivo with anti-mitotic agents, but this method is expensive, complex and time consuming. Therefore, spontaneous doubling of chromosomes during culture is preferred. In this study, the effects of colchicine use on embryo yield and embryo development in pepper (Capsicum annuum L.) anther culture were investigated. Colchicine is a chemical agent that changes the cell division process by inhibiting microtubule formation in plant cells. Colchicine was applied to pepper anthers at concentrations of 0.3%, 0.4% and 0.6% in semi-solid and double-layer media for 14 and 21 days. Embryo and plant regeneration rates obtained according to media, colchicine dose and application duration were examined. According to the trial results, the highest embryo formation rate (21.03%) was obtained in the group treated with 0.6% colchicine dose for 14 days in semi-solid media. The development rates of embryos into plants varied depending on both colchicine doses and nutrient media used. Plants developed from embryos were grouped as haploid (H) and spontaneous double haploid (SDH) by examining the presence of pollen in flowers, seeds in fruits, stomata number and stomata characteristics. It was observed that the addition of colchicine to the medium had a very significant effect on the SDH plant ratio. The highest SDH plant ratio (74.28%) was obtained from the application of 0.6% colchicine for 21 days in semi-solid medium. On the other hand, the highest SDH plant ratio (69.05%) in double-layer media was determined in the application of 0.6% colchicine dose for 14 days. The SDH plant ratio obtained from embryos grown in colchicine-free medium was found to be 48.89% (14 days) and 44.74% (21 days). It can be concluded that colchicine application significantly enhances the production of SDH plants in pepper anther culture, with the most effective treatment being 0.6% colchicine for 21 days in a semi-solid medium.

Keywords

Haploidy colchicine anther culture androgenesis chromosome doubling

Project Number

PTKTR Ar-Ge 2023_1

References

  • Alremi, F., Taskin, H., Sonmez, K., Buyukalaca, S., & Ellialtioglu, Ş. (2014). Effects of genotype and nutrient medium on anther culture in pepper (Capsicum annuum L.). Turkish Journal of Agricultural and Natural Sciences, 1(2), 108-116. https://doi.org/10.30910/turkjans.160702
  • Arı, E., Yıldırım, T., Mutlu, N., Buyukalaca, S., Gokmen, Ü., & Akman, E. (2016). Comparison of different androgenesis protocols for doubled haploid plant production in ornamental pepper (Capsicum annuum L.). Turkish Journal of Biology, 40(4), 944-954. https://doi.org/10.3906/biy- 1509-36
  • Aryavand, M., Mozafari, J., & Bagheri, A. (2003). Morphological comparison of haploid and diploid plants in anther culture of pepper (Capsicum annuum L.). Euphytica, 132(1), 25–30. https://doi.org/10.1023/A:1026088629924
  • Bat, H., Altındağ, F. N., Yiğit, M. A., Ellialtıoglu, Ş. Ş., & Comlekcioglu, N. (2021). Ploidy estimation in pepper and eggplant via stomata characteristics. International Journal of Agriculture Forestry and Life Sciences, 5(2), 139-146.
  • Castañeda-Nava, J. J., Quiroz-Figueroa, F., & Zavala-García, F. (2023). Chromosome doubling efficiency and morphological variation in doubled haploids of Capsicum chinense Jacq. Scientia Horticulturae, 312, 111974. https://doi.org/10.1016/j.scienta.2022.111974
  • Comlekcioglu, N. (2021). Effect of colchicine addition to culture medium on induction of androgenesis in pepper (Capsicum annuum L.). Pak. J. Bot, 53(3), 1001-1005. http://dx.doi.org/10.30848/PJB2021-3(14)
  • Comlekcioglu, N., & Ellialtıoglu, Ş. Ş. (2018). Review on the research carried out on in vitro androgenesis of peppers (Capsicum annuum L.) in Turkey. Research Journal of Biotechnology, 13(6), 75-84.
  • Comlekcioglu, N., & Ozden, M. (2019). Polyploid induction by colchicine treatment in goldenberry (Physalis peruviana), and effects of polyploidy on certain traits. Journal of Animal & Plant Sciences, 29(5), 1336–1343.
  • Comlekcioglu, N., Buyukalaca, S., & Abak, K. (2001). Effect of silver nitrate on haploid embryo induction by anther culture in pepper (Capsicum annuum L.). In XIth EUCARPIA meeting on genetics and breeding of capsicum and eggplant, (pp. 133-136). Antalya, Turkey.
  • Cramer, C. S. (1999).Phenotypic comparison of diploid, haploid, and doubled haploid onion (Allium cepa L.) lines. HortScience, 34(7), 1263– 1266.
  • Da Silva, H. A., Scapim, C. A., Vivas, J. M. S., do Amaral, A. T., Pinto, R. J. B., Mourão, K. S. M., & Baleroni, A. G. (2020). Effect of ploidy level on guard cell length and use of stomata to discard diploids among putative haploids in maize. Crop Science, 60(3), 1199-1209.
  • Dewitte, W., & Murray, J. A. (2003). The plant cell cycle. Annual Review of Plant Biology, 54(1), 235-264. https://doi.org/10.1146/annurev.arplant.54.031902.134836
  • Dhooghe, E., Van Laere, K., Eeckhaut, T., Leus, L., & Van Huylenbroeck, J. (2011). Mitotic chromosome doubling of plant tissues in vitro. Plant Cell, Tissue and Organ Culture, 104, 359-373. https://doi.org/10.1007/s11240- 010-9786-5
  • Ebrahimzadeh, H., Lotfi, M., & Sadat-Hosseini, M. (2021). Parthenogenetic haploid plant production in styrian pumpkin by gamma irradiated pollen. International Journal of Horticultural Science and Technology, 8(3), 305-314.
  • Ercan, N., Sensoy, F. A., & Sensoy, A. S. (2006). Influence of growing season and donor plant age on anther culture response of some pepper cultivars (Capsicum annuum L.). Scientia Horticulturae, 110(1), 16- 20.
  • Fomicheva, M.; Kulakov, Y.; Alyokhina, K.; Domblides, E.(2024). Spontaneous and chemically induced genome doubling and polyploidization in vegetable crops. Horticulturae, 10, 551. https://doi.org/10.3390/horticulturae10060551
  • Gémesné Juhász, A., Petus, M., Venczel, G., Zatykó, L., Gyulai, G., & Cséplö, M. (2000, July). Genetic variability of anther donor versus spontaneous doubled haploid descendents and colchicine induced doubled haploid sweet pepper (Capsicum annuum L.) lines. In IV International Symposium on In Vitro Culture and Horticultural Breeding 560 (pp. 149-152).
  • Grozeva, S., Pasev, G., Radeva-Ivanova, V., Todorova, V., Ivanova, V., & Nankar, A. N. (2021). Double haploid development and assessment of androgenic competence of balkan pepper core collection in Bulgaria. Plants, 10(11), 2414.
  • Gursoy, E., Yildiz, B. N., Yapici, B., & Ellialtioglu, S. S. (2022). Genotype effect on obtaining haploid embryos via anther culture in capia pepper gene pool. Bahce 51 (Special Issue 1): 294–299 ISSN: 1300-8943.
  • Gyulai, G., Gemesne, J. A., Sági, Z. S., Venczel, G., Pintér, P., Kristóf, Z. & Zatykó, L. (2000). Doubled haploid development and PCR-analysis of F1 hybrid derived DH-R2 paprika (Capsicum annuum L.) lines. Journal of Plant Physiology, 156(2), 168-174.
  • Hannweg, K., Sippel, A., & Bertling, I. (2013). A simple and effective method for the micropropagation and in vitro induction of polyploidy and the effect on floral characteristics of the South African iris, Crocosmia aurea. South African Journal of Botany, 88, 367-372.
  • Hooghvorst, I., & Nogués, S. (2021). Chromosome doubling methods in doubled haploid and haploid inducer-mediated genome-editing systems in major crops. Plant Cell Reports, 40(2), 255-270.
  • Hooghvorst, I., Torrico, O., Hooghvorst, S., & Nogués, S. (2020). In situ parthenogenetic doubled haploid production in melon “Piel de Sapo” for breeding purposes. Frontiers in Plant Science, 11, 378.
  • İlhan, M., & Kurtar, E. S. (2022). Doublehaploidization efficiency of selected pepper genotypes via in vitro anther culture. Selcuk Journal of Agriculture and Food Sciences, 36(2), 253-259. https://doi.org/10.15316/SJAFS.2022.033
  • Joubès, J., & Chevalier, C. (2000). Endoreduplication in higher plants. The Plant Cell Cycle, 191-201.
  • Kanmaz, M. G. (2021). Effects of anther and two different shed- microspore cultures on pure line production in capsicum and dolma types in pepper (Capsicum annuum L.) breeding. [Master's Thesis, Akdeniz University, Institute of Science]. YÖK National Thesis Center. https://tez.yok.gov.tr/UlusalTezMerkezi/tezSorguSonucYeni.jsp
  • Kara, E.; Taskın, H.; Karabıyık, ¸S.; Solmaz, ˙I.; Sarı, N.; Karaköy, T.; Baktemur, G. (2024). Are cytological and morphological analyses sufficient in ploidy determination of watermelon haploid plants. Horticulturae, 10, 818. https://doi.org/10.3390/horticulturae10080818
  • Kasha, K. J. (2005). Chromosome doubling and recovery of doubled haploid plants. Springer, In Haploids In Crop Improvement II (pp. 123- 152).
  • Keleş, D., Pınar, H., Ata, A., Taşkın, H., Yıldız, S., & Buyukalaca, S. (2015). Effect of pepper types on obtaining spontaneous doubled haploid plants via anther culture. HortScience, 50(11), 1671-1676.
  • Marin-Montes, I. M., Rodríguez-Pérez, J. E., Robledo-Paz, A., de la Cruz- Torres, E., Peña-Lomelí, A., & Sahagún- Castellanos, J. (2022). Haploid induction in tomato (Solanum lycopersicum L.) via gynogenesis. Plants, 11(12), 1595.
  • Mirzaei, M., Kahrizi, D., & Rezaeizad, A. (2011). Androgenesis and spontaneous chromosome doubling in Hordeum vulgare L. In Researches of the First International Conference. Babylon and Razı Universities, Euphrates J Agric Sci, 3(9), 248–252.
  • Misal, S., & Das, A. (2023). Development of a unique protocol for the production of doubled haploids in hot pepper. https://doi.org/10.21203/rs.3.rs-3294597/v1
  • Mishra, A.K., Saini, R., Tiwari, K.N. (2021). Double haploid production and its applications in crop improvement. In: Kumar Srivastava, D., Kumar Thakur, A., Kumar, P. (eds) Agricultural Biotechnology: Latest Research and Trends . Springer, Singapore. https://doi.org/10.1007/978-981-16- 2339-4_4
  • Mityko, J., Andrasfalvy, A., Csillery, G., & Fári, M. (1995). Anther‐culture response in different genotypes and F1 hybrids of pepper (Capsicum annuum L.). Plant Breeding, 114(1), 78-80.
  • Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum,15, 473- 497.
  • Niklas-Nowak, A., Olszewska, D., Kisiała, A., & Nowaczyk, P. (2012). Study of individual plant responsiveness in anther cultures of selected pepper (spp.) genotypes. Folia Horticulturae, 24(2), 141-146.
  • Omidbaigi, R., Yavari, S., Hassani, M. E., & Yavari, S. (2010). Induction of autotetraploidy in dragonhead (Dracocephalum moldavica L.) by colchicine treatment.. J. Fruit Ornam. Plant Res, 18(1), 23-35.
  • Przywara, L., Manteuffel, R., & Szczygieł, A. (1988). Induction of haploids and double haploids in anther cultures of Capsicum annuum L. Euphytica, 40(1), 91-96. https://doi.org/10.1007/BF00034535
  • Salehian, H., Shahnazi, S., & Nazari, M. (2023). Production of doubled haploid plants in cucumber (Cucumis sativus L.) via parthenogenesis. In Vitro Cellular & Developmental Biology-Plant, 59(4), 467-474.
  • Sato, S., Katoh, N., Iwai, S., & Hagimori, M. (2005). Frequency of spontaneous polyploidization of embryos regenerated from cultured anthers or microspores of Brassica rapa var. pekinensis L. and B. oleracea var. capitata L. Breeding Science, 55(1), 99-102.
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Year 2025, Volume: 15 Issue: 1, 76 - 90, 26.06.2025

Beyza Nur Marangoz , Nuray Çömlekçioğlu , Ezgi Gürsoy , Büşra Yapici , Şeküre Şebnem Ellialtıoğlu

https://doi.org/10.53518/mjavl.1583740

Abstract

Ethical Statement

Proje konusu etik izin belgesine ihtiyaç duymamaktadır. Etik ilkelere uygun çalışma yapılmış, atıf gösterilmiş ve benzerlik oranı %10'un altında kalmıştır.

Supporting Institution

Petektar Tohumculuk

Project Number

PTKTR Ar-Ge 2023_1

Thanks

Bu çalışma, PETEKTAR TOHUMCULUK AR-GE MERKEZİ tarafından özkaynaklı proje kapsamında gerçekleştirilmiştir. Beyza Nur Marangoz (Yıldız) tarafından, Prof. Dr. Nuray Çömlekçioğlu danışmanlığında yürütülen bir Yüksek Lisans Tezinden elde edilen sonuçların bir kısmından hazırlanmıştır. Firma Ar-Ge personeline teşekkür ederiz.

References

  • Alremi, F., Taskin, H., Sonmez, K., Buyukalaca, S., & Ellialtioglu, Ş. (2014). Effects of genotype and nutrient medium on anther culture in pepper (Capsicum annuum L.). Turkish Journal of Agricultural and Natural Sciences, 1(2), 108-116. https://doi.org/10.30910/turkjans.160702
  • Arı, E., Yıldırım, T., Mutlu, N., Buyukalaca, S., Gokmen, Ü., & Akman, E. (2016). Comparison of different androgenesis protocols for doubled haploid plant production in ornamental pepper (Capsicum annuum L.). Turkish Journal of Biology, 40(4), 944-954. https://doi.org/10.3906/biy- 1509-36
  • Aryavand, M., Mozafari, J., & Bagheri, A. (2003). Morphological comparison of haploid and diploid plants in anther culture of pepper (Capsicum annuum L.). Euphytica, 132(1), 25–30. https://doi.org/10.1023/A:1026088629924
  • Bat, H., Altındağ, F. N., Yiğit, M. A., Ellialtıoglu, Ş. Ş., & Comlekcioglu, N. (2021). Ploidy estimation in pepper and eggplant via stomata characteristics. International Journal of Agriculture Forestry and Life Sciences, 5(2), 139-146.
  • Castañeda-Nava, J. J., Quiroz-Figueroa, F., & Zavala-García, F. (2023). Chromosome doubling efficiency and morphological variation in doubled haploids of Capsicum chinense Jacq. Scientia Horticulturae, 312, 111974. https://doi.org/10.1016/j.scienta.2022.111974
  • Comlekcioglu, N. (2021). Effect of colchicine addition to culture medium on induction of androgenesis in pepper (Capsicum annuum L.). Pak. J. Bot, 53(3), 1001-1005. http://dx.doi.org/10.30848/PJB2021-3(14)
  • Comlekcioglu, N., & Ellialtıoglu, Ş. Ş. (2018). Review on the research carried out on in vitro androgenesis of peppers (Capsicum annuum L.) in Turkey. Research Journal of Biotechnology, 13(6), 75-84.
  • Comlekcioglu, N., & Ozden, M. (2019). Polyploid induction by colchicine treatment in goldenberry (Physalis peruviana), and effects of polyploidy on certain traits. Journal of Animal & Plant Sciences, 29(5), 1336–1343.
  • Comlekcioglu, N., Buyukalaca, S., & Abak, K. (2001). Effect of silver nitrate on haploid embryo induction by anther culture in pepper (Capsicum annuum L.). In XIth EUCARPIA meeting on genetics and breeding of capsicum and eggplant, (pp. 133-136). Antalya, Turkey.
  • Cramer, C. S. (1999).Phenotypic comparison of diploid, haploid, and doubled haploid onion (Allium cepa L.) lines. HortScience, 34(7), 1263– 1266.
  • Da Silva, H. A., Scapim, C. A., Vivas, J. M. S., do Amaral, A. T., Pinto, R. J. B., Mourão, K. S. M., & Baleroni, A. G. (2020). Effect of ploidy level on guard cell length and use of stomata to discard diploids among putative haploids in maize. Crop Science, 60(3), 1199-1209.
  • Dewitte, W., & Murray, J. A. (2003). The plant cell cycle. Annual Review of Plant Biology, 54(1), 235-264. https://doi.org/10.1146/annurev.arplant.54.031902.134836
  • Dhooghe, E., Van Laere, K., Eeckhaut, T., Leus, L., & Van Huylenbroeck, J. (2011). Mitotic chromosome doubling of plant tissues in vitro. Plant Cell, Tissue and Organ Culture, 104, 359-373. https://doi.org/10.1007/s11240- 010-9786-5
  • Ebrahimzadeh, H., Lotfi, M., & Sadat-Hosseini, M. (2021). Parthenogenetic haploid plant production in styrian pumpkin by gamma irradiated pollen. International Journal of Horticultural Science and Technology, 8(3), 305-314.
  • Ercan, N., Sensoy, F. A., & Sensoy, A. S. (2006). Influence of growing season and donor plant age on anther culture response of some pepper cultivars (Capsicum annuum L.). Scientia Horticulturae, 110(1), 16- 20.
  • Fomicheva, M.; Kulakov, Y.; Alyokhina, K.; Domblides, E.(2024). Spontaneous and chemically induced genome doubling and polyploidization in vegetable crops. Horticulturae, 10, 551. https://doi.org/10.3390/horticulturae10060551
  • Gémesné Juhász, A., Petus, M., Venczel, G., Zatykó, L., Gyulai, G., & Cséplö, M. (2000, July). Genetic variability of anther donor versus spontaneous doubled haploid descendents and colchicine induced doubled haploid sweet pepper (Capsicum annuum L.) lines. In IV International Symposium on In Vitro Culture and Horticultural Breeding 560 (pp. 149-152).
  • Grozeva, S., Pasev, G., Radeva-Ivanova, V., Todorova, V., Ivanova, V., & Nankar, A. N. (2021). Double haploid development and assessment of androgenic competence of balkan pepper core collection in Bulgaria. Plants, 10(11), 2414.
  • Gursoy, E., Yildiz, B. N., Yapici, B., & Ellialtioglu, S. S. (2022). Genotype effect on obtaining haploid embryos via anther culture in capia pepper gene pool. Bahce 51 (Special Issue 1): 294–299 ISSN: 1300-8943.
  • Gyulai, G., Gemesne, J. A., Sági, Z. S., Venczel, G., Pintér, P., Kristóf, Z. & Zatykó, L. (2000). Doubled haploid development and PCR-analysis of F1 hybrid derived DH-R2 paprika (Capsicum annuum L.) lines. Journal of Plant Physiology, 156(2), 168-174.
  • Hannweg, K., Sippel, A., & Bertling, I. (2013). A simple and effective method for the micropropagation and in vitro induction of polyploidy and the effect on floral characteristics of the South African iris, Crocosmia aurea. South African Journal of Botany, 88, 367-372.
  • Hooghvorst, I., & Nogués, S. (2021). Chromosome doubling methods in doubled haploid and haploid inducer-mediated genome-editing systems in major crops. Plant Cell Reports, 40(2), 255-270.
  • Hooghvorst, I., Torrico, O., Hooghvorst, S., & Nogués, S. (2020). In situ parthenogenetic doubled haploid production in melon “Piel de Sapo” for breeding purposes. Frontiers in Plant Science, 11, 378.
  • İlhan, M., & Kurtar, E. S. (2022). Doublehaploidization efficiency of selected pepper genotypes via in vitro anther culture. Selcuk Journal of Agriculture and Food Sciences, 36(2), 253-259. https://doi.org/10.15316/SJAFS.2022.033
  • Joubès, J., & Chevalier, C. (2000). Endoreduplication in higher plants. The Plant Cell Cycle, 191-201.
  • Kanmaz, M. G. (2021). Effects of anther and two different shed- microspore cultures on pure line production in capsicum and dolma types in pepper (Capsicum annuum L.) breeding. [Master's Thesis, Akdeniz University, Institute of Science]. YÖK National Thesis Center. https://tez.yok.gov.tr/UlusalTezMerkezi/tezSorguSonucYeni.jsp
  • Kara, E.; Taskın, H.; Karabıyık, ¸S.; Solmaz, ˙I.; Sarı, N.; Karaköy, T.; Baktemur, G. (2024). Are cytological and morphological analyses sufficient in ploidy determination of watermelon haploid plants. Horticulturae, 10, 818. https://doi.org/10.3390/horticulturae10080818
  • Kasha, K. J. (2005). Chromosome doubling and recovery of doubled haploid plants. Springer, In Haploids In Crop Improvement II (pp. 123- 152).
  • Keleş, D., Pınar, H., Ata, A., Taşkın, H., Yıldız, S., & Buyukalaca, S. (2015). Effect of pepper types on obtaining spontaneous doubled haploid plants via anther culture. HortScience, 50(11), 1671-1676.
  • Marin-Montes, I. M., Rodríguez-Pérez, J. E., Robledo-Paz, A., de la Cruz- Torres, E., Peña-Lomelí, A., & Sahagún- Castellanos, J. (2022). Haploid induction in tomato (Solanum lycopersicum L.) via gynogenesis. Plants, 11(12), 1595.
  • Mirzaei, M., Kahrizi, D., & Rezaeizad, A. (2011). Androgenesis and spontaneous chromosome doubling in Hordeum vulgare L. In Researches of the First International Conference. Babylon and Razı Universities, Euphrates J Agric Sci, 3(9), 248–252.
  • Misal, S., & Das, A. (2023). Development of a unique protocol for the production of doubled haploids in hot pepper. https://doi.org/10.21203/rs.3.rs-3294597/v1
  • Mishra, A.K., Saini, R., Tiwari, K.N. (2021). Double haploid production and its applications in crop improvement. In: Kumar Srivastava, D., Kumar Thakur, A., Kumar, P. (eds) Agricultural Biotechnology: Latest Research and Trends . Springer, Singapore. https://doi.org/10.1007/978-981-16- 2339-4_4
  • Mityko, J., Andrasfalvy, A., Csillery, G., & Fári, M. (1995). Anther‐culture response in different genotypes and F1 hybrids of pepper (Capsicum annuum L.). Plant Breeding, 114(1), 78-80.
  • Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum,15, 473- 497.
  • Niklas-Nowak, A., Olszewska, D., Kisiała, A., & Nowaczyk, P. (2012). Study of individual plant responsiveness in anther cultures of selected pepper (spp.) genotypes. Folia Horticulturae, 24(2), 141-146.
  • Omidbaigi, R., Yavari, S., Hassani, M. E., & Yavari, S. (2010). Induction of autotetraploidy in dragonhead (Dracocephalum moldavica L.) by colchicine treatment.. J. Fruit Ornam. Plant Res, 18(1), 23-35.
  • Przywara, L., Manteuffel, R., & Szczygieł, A. (1988). Induction of haploids and double haploids in anther cultures of Capsicum annuum L. Euphytica, 40(1), 91-96. https://doi.org/10.1007/BF00034535
  • Salehian, H., Shahnazi, S., & Nazari, M. (2023). Production of doubled haploid plants in cucumber (Cucumis sativus L.) via parthenogenesis. In Vitro Cellular & Developmental Biology-Plant, 59(4), 467-474.
  • Sato, S., Katoh, N., Iwai, S., & Hagimori, M. (2005). Frequency of spontaneous polyploidization of embryos regenerated from cultured anthers or microspores of Brassica rapa var. pekinensis L. and B. oleracea var. capitata L. Breeding Science, 55(1), 99-102.
  • Seguí-Simarro, J. M., & Nuez, F. (2008). Pathways to doubled haploidy: chromosome doubling during androgenesis. Cytogenetic and Genome Research, 120(3-4), 358-369.
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There are 52 citations in total.

Details

Primary Language English
Subjects Vegetable Growing and Treatment
Journal Section Research Article
Authors

Beyza Nur Marangoz 0000-0003-4323-1864

Nuray Çömlekçioğlu 0000-0001-7189-613X

Ezgi Gürsoy 0000-0002-4920-8027

Büşra Yapici 0000-0003-4460-1409

Şeküre Şebnem Ellialtıoğlu 0000-0002-3851-466X

Project Number PTKTR Ar-Ge 2023_1
Publication Date June 26, 2025
Submission Date November 12, 2024
Acceptance Date June 4, 2025
Published in Issue Year 2025 Volume: 15 Issue: 1

Cite

APA Marangoz, B. N., Çömlekçioğlu, N., Gürsoy, E., Yapici, B., et al. (2025). Effects of Colchicine Applications on Embryo Yield and Spontaneous Chromosome Doubling in Pepper (Capsicum annuum L.) Anther Culture. Manas Journal of Agriculture Veterinary and Life Sciences, 15(1), 76-90. https://doi.org/10.53518/mjavl.1583740
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