Research Article
BibTex RIS Cite

Analysis of the binary mixtures of amlodipine and atorvastatin by chemometric-spectophotometric method

Year 2025, Volume: 13 Issue: 1, 2 - 7, 27.06.2025

Güzide Pekcan , Tuğba Karaca Erol , Hayrettin Bulum

https://doi.org/10.51354/mjen.1389474

Abstract

In this study, the simultaneous quantification of amlodipine and atorvastatin in pharmaceutical preparations was successfully accomplished without the need for a separation process by using chemometric calibration methods (principal component analysis method (PCA), principal component regression method (PCR), and partial least squares method (PLS). Additionally, chemometric evaluation was performed on the data obtained from UV visible field spectroscopy methods. 16 mixes containing the chemicals atorvastatin and amlodipine were created as a calibration (concentration) set to assess the validity of the designated chemometric procedures. The calibration set's absorption spectrum was captured between 200 and 500 nm. The link between the calibration set and the absorption data collected in the 215–355 nm region was used to produce three chemometric calibrations. Amlodipine and atorvastatin synthetic mixtures were analyzed to assess the validity of the PCA, PCR, and PLS approaches. The percentage (%) recovery average for atorvastatin and amlodipine was determined for both the drug sample and the synthetic mixture using the approach we used. It was discovered that the computed values were fairly high.The methodologies were controlled, and the ANOVA test was run using the results of the PLS and PCR calibration techniques. The enhanced technique might be suggested for the examination of medication samples containing atorvastatin and amlodipine since it is among the most sensitive, accurate, and repeatable techniques available.

Keywords

Amlodipine Atorvastatin Chemometry

Supporting Institution

Suleyman Demirel University

Project Number

FYL-2021-8323

Thanks

This research work has been supported by research grants from Suleyman Demirel University Scientific Research Project FYL-2021-8323.

References

  • [1]. Kalan M.K., “Hipertansiyon Hastalarında Antihipertansif İlaç Değişimleri, Bu Değişimlerin Nedenleri ve İlaç Değişimleri ile Hedef Tedavi Değerlerine Ulaşma Oranlarının İncelenmesi”. Sağlık Bakanlığı Şişli Etfal Eğitim ve Araştırma Hastanesi Aile Hekimliği Koordinatörlüğü, Uzmanlık Tezi, İstanbul, 2007, pp.98.
  • [2]. Yılmaz N., “Amlodipin ve Rosuvastatin Etkin Maddelerinin Farmasötik Preparatlarda Kare Dalga Voltametri Yöntemi ile Aynı Anda Miktar Tayini”. Atatürk Üniversitesi Sağlık Bilimleri Enstitüsü, Yüksek Lisans Tezi, Erzurum, 2018, pp. 2-3.
  • [3]. Tomikj M., Božinovska, Anevska-Stojanovska N., Lazova J., Acevska J., Brezovska K., Tonich-Ribarska J., Nakov N., “Sustainable and white HPLC method for simultaneous determination of amlodipine and atorvastatin in film-coated tablet”,Green Analytical Chemistry, 8, 100103.
  • [4]. Reid I.O.A., Mohamed M.E., “Chemometric Simultaneous Determination of Atorvastatin and Amlodipine in Bulk and Tablets”, International Journal Pharmacy and Chemistry, 5(4), (2019), 42-47.
  • [5]. Mansour F.R, “A New Innovative Spectrophotometric Method For The Simultaneous Determination of Sofosbuvir and Ledipasvir”. Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy 18(188), (2018), 626-632.
  • [6]. Dinç E., Baleanu D., “Spectrophotometric Quantitative Determination of Cilazapril and Hydrochlorothiazide in Tablets By Chemometric Methods”. J. Pharmaceut. Biomed, 30, (2002), 715-723.
  • [7]. Kenneth R, Chemometrics: A practical Guide. John Wiley & Sons. Inc., New York, 1997.
  • [8]. “Minitab 17 Statistical Programme” http://www.inovadanismanlik.com.tr (06.11.2023).
  • [9]. Dinç E., , “Kemometrik İşlem ve Yöntemlerin Analitik Kimyadaki Tipik Uygulamaları”, Uygulamalı Kemometri Yaz Okulu Notları, 2009, pp. 13-17.
  • [10]. Dinç E., Baleanu D., “ Spectrophotometric quantitative determination of cilazapril and hydrochlorothiazide in tablets by chemometric methods” Journal of pharmaceutical and biomedical analysis, 30, (2002), 715-723.
  • [11]. Dinç E., ,Özdemir, A., Baleanu, D., “Comparative study of the continuous wavelent transform, derivative and partial least squares methods applied to the overlapping spectra for the simultaneous quantitative resolution of ascorbic acid and asetilsalicylic acid in effervescent tablets, Journal of pharmaceutical and biomedical analysis, 37, (2005), 715-723.
  • [12]. Ustündağ, O., & Dinç, E., “Simultaneous resolution of a binary mixture of captopril and hydrochlorothiazide in tablets by bivariate and multivariate spectral calibrations”. Die Pharmazie, 58 (9), (2003), 623-8 .
  • [13]. Dinç, E., Ustündağ, O., & Baleanu, D. (2010). “Simultaneous chemometric determination of pyridoxine hydrochloride and isoniazid in tablets by multivariate regression methods“.. Drug testing and analysis, 2 (8), (2010), 383-387.
  • [14]. Porfire A, Muntean D, Achim M, Vlase L, Tomuta, I, “Simultaneous Quantification of Simvastatin and Excipients in Liposomes Using Near Infrared Spectroscopy and Chemometry”. Journal of Pharmaceutical and Biomedical Analysis 107, (2015), 40-49.
  • [15]. Tarhan I, Kara AAIH, “Quantitative Determination of Free Fatty Acids in Extra Virgin Olive Oils by Multivariate Methods and Fourier Transform Infrared Spectroscopy Considering Different Absorption Modes”. International Journal of Food Properties (2017), 1-8.
  • [16]. Abbai N, Parameswari SA, “Stability Indicating RP-HPLC Method Development and Validation for Simultaneous Estimation od Salbutamol and Beclomethasone in Bulk and Tablet Dosage Form”, IJPSR 9(5), (2009), 1980-1988.
  • [17]. Aravind D, Kamarapu SK, “Method Development and Validation of RP-HPLC Method for Simultaneous Estimation of Clidinium Bromide, Chlordiazepoxide and Dicylomine Hydrochloride in Bulk and Combined Tablet Dosage Forms”, IJPBS 3(3), (2013), 152-161.
  • [18]. Deshpande P, Mandawad V, “Development and Validation of Stability Indicating HPTLC Method for Determination of Azelastine Hydrochloride as Bulk Drug and in Pharmaceutical Liquid Dosage Form”, IAJPS 05(06), (2018), 5107-5113.
  • [19]. Bilgili AV, Çullu MA, Aydemir S, “Tuzdan Etkilenmiş Toprakların Yakın Kızılötesi Yansıma Spektroradyometre ve Elektromanyetik Indüksiyon Tekniği Yardımıyla Karakterize Edilebilme Potansiyelinin Araştırılması”. Harran Tarım ve Gıda Bilimleri Dergisi 18(1), (2014), 32-45.
  • [20]. Shrivastava A, Gupta VB, “Methods For The Determination of Limit of Detection and Limit of Quantitation of The Analytical Methods”. Chronicles of Young Scientist 2(1), (2011), 21-25.
  • [21]. Armbruster DA, Pty T, “Limit of Blank, Limit of Detection and Limit of Quantitation”. Clin. Niochem Rev. 29, (2008), 49-52

Year 2025, Volume: 13 Issue: 1, 2 - 7, 27.06.2025

Güzide Pekcan , Tuğba Karaca Erol , Hayrettin Bulum

https://doi.org/10.51354/mjen.1389474

Abstract

Project Number

FYL-2021-8323

References

  • [1]. Kalan M.K., “Hipertansiyon Hastalarında Antihipertansif İlaç Değişimleri, Bu Değişimlerin Nedenleri ve İlaç Değişimleri ile Hedef Tedavi Değerlerine Ulaşma Oranlarının İncelenmesi”. Sağlık Bakanlığı Şişli Etfal Eğitim ve Araştırma Hastanesi Aile Hekimliği Koordinatörlüğü, Uzmanlık Tezi, İstanbul, 2007, pp.98.
  • [2]. Yılmaz N., “Amlodipin ve Rosuvastatin Etkin Maddelerinin Farmasötik Preparatlarda Kare Dalga Voltametri Yöntemi ile Aynı Anda Miktar Tayini”. Atatürk Üniversitesi Sağlık Bilimleri Enstitüsü, Yüksek Lisans Tezi, Erzurum, 2018, pp. 2-3.
  • [3]. Tomikj M., Božinovska, Anevska-Stojanovska N., Lazova J., Acevska J., Brezovska K., Tonich-Ribarska J., Nakov N., “Sustainable and white HPLC method for simultaneous determination of amlodipine and atorvastatin in film-coated tablet”,Green Analytical Chemistry, 8, 100103.
  • [4]. Reid I.O.A., Mohamed M.E., “Chemometric Simultaneous Determination of Atorvastatin and Amlodipine in Bulk and Tablets”, International Journal Pharmacy and Chemistry, 5(4), (2019), 42-47.
  • [5]. Mansour F.R, “A New Innovative Spectrophotometric Method For The Simultaneous Determination of Sofosbuvir and Ledipasvir”. Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy 18(188), (2018), 626-632.
  • [6]. Dinç E., Baleanu D., “Spectrophotometric Quantitative Determination of Cilazapril and Hydrochlorothiazide in Tablets By Chemometric Methods”. J. Pharmaceut. Biomed, 30, (2002), 715-723.
  • [7]. Kenneth R, Chemometrics: A practical Guide. John Wiley & Sons. Inc., New York, 1997.
  • [8]. “Minitab 17 Statistical Programme” http://www.inovadanismanlik.com.tr (06.11.2023).
  • [9]. Dinç E., , “Kemometrik İşlem ve Yöntemlerin Analitik Kimyadaki Tipik Uygulamaları”, Uygulamalı Kemometri Yaz Okulu Notları, 2009, pp. 13-17.
  • [10]. Dinç E., Baleanu D., “ Spectrophotometric quantitative determination of cilazapril and hydrochlorothiazide in tablets by chemometric methods” Journal of pharmaceutical and biomedical analysis, 30, (2002), 715-723.
  • [11]. Dinç E., ,Özdemir, A., Baleanu, D., “Comparative study of the continuous wavelent transform, derivative and partial least squares methods applied to the overlapping spectra for the simultaneous quantitative resolution of ascorbic acid and asetilsalicylic acid in effervescent tablets, Journal of pharmaceutical and biomedical analysis, 37, (2005), 715-723.
  • [12]. Ustündağ, O., & Dinç, E., “Simultaneous resolution of a binary mixture of captopril and hydrochlorothiazide in tablets by bivariate and multivariate spectral calibrations”. Die Pharmazie, 58 (9), (2003), 623-8 .
  • [13]. Dinç, E., Ustündağ, O., & Baleanu, D. (2010). “Simultaneous chemometric determination of pyridoxine hydrochloride and isoniazid in tablets by multivariate regression methods“.. Drug testing and analysis, 2 (8), (2010), 383-387.
  • [14]. Porfire A, Muntean D, Achim M, Vlase L, Tomuta, I, “Simultaneous Quantification of Simvastatin and Excipients in Liposomes Using Near Infrared Spectroscopy and Chemometry”. Journal of Pharmaceutical and Biomedical Analysis 107, (2015), 40-49.
  • [15]. Tarhan I, Kara AAIH, “Quantitative Determination of Free Fatty Acids in Extra Virgin Olive Oils by Multivariate Methods and Fourier Transform Infrared Spectroscopy Considering Different Absorption Modes”. International Journal of Food Properties (2017), 1-8.
  • [16]. Abbai N, Parameswari SA, “Stability Indicating RP-HPLC Method Development and Validation for Simultaneous Estimation od Salbutamol and Beclomethasone in Bulk and Tablet Dosage Form”, IJPSR 9(5), (2009), 1980-1988.
  • [17]. Aravind D, Kamarapu SK, “Method Development and Validation of RP-HPLC Method for Simultaneous Estimation of Clidinium Bromide, Chlordiazepoxide and Dicylomine Hydrochloride in Bulk and Combined Tablet Dosage Forms”, IJPBS 3(3), (2013), 152-161.
  • [18]. Deshpande P, Mandawad V, “Development and Validation of Stability Indicating HPTLC Method for Determination of Azelastine Hydrochloride as Bulk Drug and in Pharmaceutical Liquid Dosage Form”, IAJPS 05(06), (2018), 5107-5113.
  • [19]. Bilgili AV, Çullu MA, Aydemir S, “Tuzdan Etkilenmiş Toprakların Yakın Kızılötesi Yansıma Spektroradyometre ve Elektromanyetik Indüksiyon Tekniği Yardımıyla Karakterize Edilebilme Potansiyelinin Araştırılması”. Harran Tarım ve Gıda Bilimleri Dergisi 18(1), (2014), 32-45.
  • [20]. Shrivastava A, Gupta VB, “Methods For The Determination of Limit of Detection and Limit of Quantitation of The Analytical Methods”. Chronicles of Young Scientist 2(1), (2011), 21-25.
  • [21]. Armbruster DA, Pty T, “Limit of Blank, Limit of Detection and Limit of Quantitation”. Clin. Niochem Rev. 29, (2008), 49-52
There are 21 citations in total.

Details

Primary Language English
Subjects Chemical Engineering (Other)
Journal Section Research Article
Authors

Güzide Pekcan 0000-0001-9230-5634

Tuğba Karaca Erol 0000-0001-5065-2531

Hayrettin Bulum 0000-0001-9841-2473

Project Number FYL-2021-8323
Publication Date June 27, 2025
Submission Date November 11, 2023
Acceptance Date March 12, 2025
Published in Issue Year 2025 Volume: 13 Issue: 1

Cite

APA Pekcan, G., Karaca Erol, T., & Bulum, H. (2025). Analysis of the binary mixtures of amlodipine and atorvastatin by chemometric-spectophotometric method. MANAS Journal of Engineering, 13(1), 2-7. https://doi.org/10.51354/mjen.1389474
AMA Pekcan G, Karaca Erol T, Bulum H. Analysis of the binary mixtures of amlodipine and atorvastatin by chemometric-spectophotometric method. MJEN. June 2025;13(1):2-7. doi:10.51354/mjen.1389474
Chicago Pekcan, Güzide, Tuğba Karaca Erol, and Hayrettin Bulum. “Analysis of the Binary Mixtures of Amlodipine and Atorvastatin by Chemometric-Spectophotometric Method”. MANAS Journal of Engineering 13, no. 1 (June 2025): 2-7. https://doi.org/10.51354/mjen.1389474.
EndNote Pekcan G, Karaca Erol T, Bulum H (June 1, 2025) Analysis of the binary mixtures of amlodipine and atorvastatin by chemometric-spectophotometric method. MANAS Journal of Engineering 13 1 2–7.
IEEE G. Pekcan, T. Karaca Erol, and H. Bulum, “Analysis of the binary mixtures of amlodipine and atorvastatin by chemometric-spectophotometric method”, MJEN, vol. 13, no. 1, pp. 2–7, 2025, doi: 10.51354/mjen.1389474.
ISNAD Pekcan, Güzide et al. “Analysis of the Binary Mixtures of Amlodipine and Atorvastatin by Chemometric-Spectophotometric Method”. MANAS Journal of Engineering 13/1 (June 2025), 2-7. https://doi.org/10.51354/mjen.1389474.
JAMA Pekcan G, Karaca Erol T, Bulum H. Analysis of the binary mixtures of amlodipine and atorvastatin by chemometric-spectophotometric method. MJEN. 2025;13:2–7.
MLA Pekcan, Güzide et al. “Analysis of the Binary Mixtures of Amlodipine and Atorvastatin by Chemometric-Spectophotometric Method”. MANAS Journal of Engineering, vol. 13, no. 1, 2025, pp. 2-7, doi:10.51354/mjen.1389474.
Vancouver Pekcan G, Karaca Erol T, Bulum H. Analysis of the binary mixtures of amlodipine and atorvastatin by chemometric-spectophotometric method. MJEN. 2025;13(1):2-7.
 Download Cover Image

Manas Journal of Engineering 

16155