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Year 2025, Volume: 10 Issue: 1, 529 - 538, 01.04.2025
https://doi.org/10.28978/nesciences.1661006

Abstract

References

  • Abdulkareem, A. A. (2023, December). Effect of Media Type and Cytokinines on Micropropagation of Two Cultivars of Pomegranate Punica granatum L. In IOP Conference Series: Earth and Environmental Science (Vol. 1262, No. 4, p. 042010). IOP Publishing. https://doi.org/10.1088/1755-1315/1262/4/042010
  • Abdulkareem, Ali Adil, and Nahla Hamoody Hussien. "Effect of Foliar Spray with Yeast Suspension and Foliartal Nutrient Solution on the Mineral Content of Tissue Lime Seedlings Citrus limon L." IOP Conference Series: Earth and Environmental Science. Vol. 1060. No. 1. IOP Publishing, 2022. https://doi.org/10.1088/1755-1315/1060/1/012056
  • Acarsoy Bilgin, N., Mısırlı, A., Belge, A., & Özen, M. (2020). The pollen and fruit properties of Ficus carica Caprificus. International Journal of Fruit Science, 20(sup3), S1696-S1705.
  • Ahmed, M. S., & Maher, S. M. (2022). A Comparative Evaluation of Machin Learning Methods in Digital Image Classification. International Journal of Advances in Engineering and Emerging Technology, 13(2), 241-253.
  • AL-Hussini, Z. A. A., Al-jibouri, A. A. M., Mahdi, A. A., Hussin, F. N., & Abbas, D. (2007). Micropropagation of kiwi Actinidia chinensis. Baghdad Science Journal, 4(2), 233-238.
  • Al-Jubori, M. T., & Al-Amery, L. (2022). Effect of cytokinins BA, Kin, auxin IAA and IBA on propagating (citrus aurantiifolia) in vitro. Int. J. Agricult. Stat. Sci, 18(1), 2033-2040.
  • Al-Jubori, M. T., Al-Dabbagh, F. M. K., & Al-Ani, E. W. (2023). Optimizing media sterilization via chlorine dioxide and autoclaving of paulowni micropropagation. Iraqi Journal of Agricultural Sciences, 54(6), 1737-1745.
  • Al-Obaidy, O. M., & Khierallah, H. S. M. (2017). The roll of some plant growth regulators on shoots multiplication of stevia plants in vitro. The Iraqi Journal of Agricultural Science, 48(5), 1158-1168.
  • Al-sumaidai, K. M. I. (2017). Applications in plant biotechnologies. University administration for printing, publishing and translation. University of Nahrain. Iraq.
  • Alwash. B.M.J. and Jassim, A, Z. 2014. Study the role of auxins and sytoknins on in vitro propagation of Spilanthes acmella (L.) Murr. Baghdad science journal.vol .11 No.3.
  • Annon, A. H., & Abdulrasool, I. J. (2020). Effect of gamma radiation and ethyl methanesulfonate (ems) on potato salt stress tolerance in vitro. The Iraqi Journal of Agricultural Science, 51(4), 982-990.
  • Bani, S. H. S., Shaaban, L., & Al-Mizory, M. (2017). Effect of Media cultures and Benzyladenine (Ba) concentrations in micropropagation of three types of Cherry Rootstock (Establishment and Multiplication). The Iraqi Journal of Agricultural Science, 48, 113. https://doi.org/10.36103/ijas.v48iSpecial.252
  • Barhoumi, E. M., Charabi, Y., & Farhani, S. (2024). Detailed guide to machine learning techniques in signal processing. Progress in Electronics and Communication Engineering, 2(1), 39–47.
  • Chauhan, R. D., & Kanwar, K. (2012). Biotechnological advances in pomegranate (Punica granatum L.). In Vitro Cellular & Developmental Biology-Plant, 48, 579-594.
  • Desai, P., Patil, G., Dholiya, B., Desai, S., Patel, F., & Narayanan, S. (2018). Development of an efficient micropropagation protocol through axillary shoot proliferation for pomegranate variety ‘Bhagwa’. Annals of Agrarian science, 16(4), 444-450. https://doi.org/10.1016/j.aasci.2018.06.002
  • Fakhrian, M., Jafariyan, M., Pirali Zefrehei, A. R., & Sahraei, H. (2022). Effect of dietary medicinal plants on some biochemical hematological parameters of sterlet (Acipenser ruthenus). International Journal of Aquatic Research and Environmental Studies, 2(1), 55-59. https://doi.org/10.70102/IJARES/V2I1/6
  • Folta, K. M., Dhingra, A., Howard, L., Stewart, P. J., & Chandler, C. K. (2006). Characterization of LF9, an octoploid strawberry genotype selected for rapid regeneration and transformation. Planta, 224, 1058-1067.
  • Ghorbani, A., & Hatami, R. 2016. In vitro micropropagation of fig (Ficus carica L.) using nodal segments. Acta Agriculturae Slovenica, 108(2), 165-175.
  • Heisler, M. G., & Byrne, M. E. (2020). Progress in understanding the role of auxin in lateral organ development in plants. Current Opinion in Plant Biology, 53, 73-79. https://doi.org/10.1016/j.pbi.2019.10.007
  • Kaji, B. V., Ershadi, A., & Tohidfar, M. (2013). In vitro propagation of pomegranate (Punica granatum 1.) Cv.'Males Yazdi'. Albanian Journal of Agricultural Sciences, 12(1), 43.
  • Kanwar, K., Joseph, J., & Deepika, R. (2010). Comparison of in vitro regeneration pathways in Punica granatum L. Plant Cell, Tissue and Organ Culture (PCTOC), 100, 199-207.
  • Kinfe, B., Feyssa, T., & Bedada, G. (2017). In vitro micropropagation of grape vine (Vitis vinifera L.) from nodal culture. African Journal of Biotechnology, 16(43), 2083-2091. https://doi.org/10.5897/AJB2016.15803
  • Kurmi, U. S., Sharma, D. K., Tripathi, M. K., Tiwari, R., Baghel, B. S., & Tiwari, S. (2011). Plant regeneration of Vitis vinifera (L) via direct and indirect organogenesis from cultured nodal segments. Journal of Agricultural Technology, 7(3), 721-737.
  • Landi, L., & Mezzetti, B. (2006). TDZ, auxin and genotype effects on leaf organogenesis in Fragaria. Plant cell reports, 25, 281-288.
  • Lloyd, G and B. McCown. 1981.Commercially feasible micropropagation of Lu, M.C. 2005. Micropropagation of Vitis thunbergii Sieb. et Zucc., a medicinal herb,through high- frequency shoot tip culture. Sci.Hortic. 107: 64–69.
  • Lloyd, G., & McCown, B. H. (1980). Mountain laurel (Kalmia latifolia) by use of shoot tip culture. Proceedings of the International Plant Propagators' Society, 30, 421–427.
  • Majid, A. (2018). The Activity of Antioxidants Enzymes and NPK Contents as Affected by Water Quality, Kinetin, Bio and Organic Fertilization in Lettuce (Lactuca sativa L.). Iraqi Journal of Agricultural Sciences, 49(3).
  • Mozafari, A. A., Ghoraishi, O., Ghaderi, N., & Javadi, T. (2016). Micropropagation of grape cultivars (Vitis vinifera L.) on different basal media supplemented with benzyl adenine. Agriculturae Conspectus Scientificus, 81(3), 123-129.
  • Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia plantarum, 15(3). https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  • Osama, S. S. (2022). Micropropagation of grapevine (Vitis Vinifera L.) Cvs. red globe and Superior. The Iraqi Journal of Agricultural Science, 53(4), 833-849.
  • Pal, R. K., Babu, K. D., Singh, N. V., Ashis Maity, A. M., & Nilesh Gaikwad, N. G. (2014). Pomegranate research in India-status and future challenges.
  • Parab, A. R., Chew, B. L., Yeow, L. C., & Subramaniam, S. (2021). Organogenesis on apical buds in common fig (Ficus carica) var. Black Jack. Electronic Journal of Biotechnology, 54, 69-76. https://doi.org/10.1016/j.ejbt.2021.10.001
  • Passey, A., Barrett, K., & James, D. (2003). Adventitious shoot regeneration from seven commercial strawberry cultivars (Fragaria× ananassa Duch.) using a range of explant types. Plant cell reports, 21, 397-401.
  • Patil, V. M., Dhande, G. A., Thigale, D. M., & Rajput, J. C. (2011). Micropropagation of pomegranate (Punica granatum L.) ‘Bhagava’cultivar from nodal explant. African journal of Biotechnology, 10(79), 18130-18136. https://doi.org/10.5897/AJB11.1437
  • Prabhuling, Guranna, & Hoolageri Huchesh. "Direct in vitro regeneration in fig (Ficus carcia L.) cv.‘Brown Turkey’." Research Journal of Biotechnology 13.5 (2018): 77-83.
  • Rajabi, A., Atashi, S., & Khosh-Khui, M. (2018). Effects of thidiazuron, auxins, and explant type on in vitro rooting of fig (Ficus carica L.) plantlets. Journal of Plant Growth Regulation, 37(4), 1314-1324.
  • Rezaei et al. (2014) demonstrated the role of TDZ in somatic embryogenesis and plantlet regeneration in fig.
  • Ružić, D., Vujović, T., Libiakova, G., Cerović, R., & Gajdošova, A. (2012). Micropropagation in vitro of highbush blueberry (Vaccinium corymbosum L.). Journal of berry research, 2(2), 97-103. https://doi.org/10.3233/JBR-2012-030
  • Sarkhosh, A. Yavari, A. Ferguson. L. 2022. The Fig Botany, Production and https://doi.org/10.1079/9781789242881.0000
  • Seifi, E., Saeedipour, S., & Jalali-Javaran, M. (2016). Micropropagation of fig (Ficus carica L.) using single-node explants. Acta Agriculturae Slovenica, 107(1), 147-156.
  • Shahatha, E. F., Al-Mousawi, A. H., & Ibrahim, K. M. (2012). The Effect of different concentration of growth regulater on micropropagtion of Lisianthus (Eustoma grandiflora). Baghdad Science Journal, 9(3), 378-385.
  • Shi, S., Caluyo, F., Hernandez, R., Sarmiento, J., & Rosales, C. A. (2024). Automatic Classification and Identification of Plant Disease Identification by Using a Convolutional Neural Network. Natural and Engineering Sciences, 9(2), 184-197. https://doi.org/10.28978/nesciences.1569560
  • Sriskanda. D iew Y.X PingKhorF.M. Chew L. 2021. An efficient micropropagation protocol for Ficus carica cv. Golden Orphan suitable for mass propagation Biocatalysis and Agricultural Biotechnology Volume 38, November.
  • Toro, J. A. R., Sampietro-Saquicela, J. L., Suryasa, I. W., Solórzano, L. E. H., Cervantes, X. L. G., & Quiñónez, B. F. C. (2024). Optimal scheduling of renewable energy resources in energy management systems using hybrid genetic algorithm and particle swarm optimization. Acta Innovations, 52, 19-27.
  • Yildiz, M. (2012). The prerequisite of the success in plant tissue culture: high frequency shoot regeneration. In Recent advances in plant in vitro culture. IntechOpen. https://doi.org/10.5772/51097

Influence Different Concentrations of Thidiazurone (TDZ) on in Vitro Micropropagation of Two Cultivars of Ficus Carica L

Year 2025, Volume: 10 Issue: 1, 529 - 538, 01.04.2025
https://doi.org/10.28978/nesciences.1661006

Abstract

A study was done to test the effect of different factors on the multiplication and growth of fig plants in vitro. They were explants excised from two different cultivars wazziri and mission cultivar locally named (Bathinjani) and inoculated on two types of media murashiage and skooge 1962 (MS) and woody plant media (WPM) results were recorded after 6 weeks, where MS media gave a highest shoot number, leaf number and shoot length (1.50, 3.10, and 2.57) respectively, Bathinjani cultivar gave maximum means of shoots and leaves number (1.5 and 2.73)respectively, Bathinjani proliferated on MS media gave highest value of same characteristic mention above (1.67, 3.20 and 2.97) respectively, explants of the best cultivar were transferred into the most efficient medium. An explant 1cm length of the Bathinjani cultivar was cultured in MS medium containing TDZ at levels (0, 0.5, 1, 1.5, 2, 2.5 and 3) mg.-l to study its effect on shoot multiplication and growth the result showed the highest shoot number and its length was 7.20 shoot, 2.33cm respectively at 2mg.l -1 with a significant increase compared with rest treatments followed by concentration at 1.5 mg.l-1 also gave significant increasing of shoot number 5.22 so the concentration at 2.5 achieved high value of shoot length was 2.20cm, then plantlet subculture on MS media of rooting stage which contain half strength MS with adding Auxines IAA and IBA at (0, 0.25, 0.5 and 1) mg.l-1 for both, the data appeared IBA and IAA at 0.5 mg.l-1 gave highest value of root number and rooted percentage reached to (2.77, 2.38) and (99.33, 96.08 ) % respectively compared with 0.0 and 1 mg.l-1 gave lowest value for same characteristics.

References

  • Abdulkareem, A. A. (2023, December). Effect of Media Type and Cytokinines on Micropropagation of Two Cultivars of Pomegranate Punica granatum L. In IOP Conference Series: Earth and Environmental Science (Vol. 1262, No. 4, p. 042010). IOP Publishing. https://doi.org/10.1088/1755-1315/1262/4/042010
  • Abdulkareem, Ali Adil, and Nahla Hamoody Hussien. "Effect of Foliar Spray with Yeast Suspension and Foliartal Nutrient Solution on the Mineral Content of Tissue Lime Seedlings Citrus limon L." IOP Conference Series: Earth and Environmental Science. Vol. 1060. No. 1. IOP Publishing, 2022. https://doi.org/10.1088/1755-1315/1060/1/012056
  • Acarsoy Bilgin, N., Mısırlı, A., Belge, A., & Özen, M. (2020). The pollen and fruit properties of Ficus carica Caprificus. International Journal of Fruit Science, 20(sup3), S1696-S1705.
  • Ahmed, M. S., & Maher, S. M. (2022). A Comparative Evaluation of Machin Learning Methods in Digital Image Classification. International Journal of Advances in Engineering and Emerging Technology, 13(2), 241-253.
  • AL-Hussini, Z. A. A., Al-jibouri, A. A. M., Mahdi, A. A., Hussin, F. N., & Abbas, D. (2007). Micropropagation of kiwi Actinidia chinensis. Baghdad Science Journal, 4(2), 233-238.
  • Al-Jubori, M. T., & Al-Amery, L. (2022). Effect of cytokinins BA, Kin, auxin IAA and IBA on propagating (citrus aurantiifolia) in vitro. Int. J. Agricult. Stat. Sci, 18(1), 2033-2040.
  • Al-Jubori, M. T., Al-Dabbagh, F. M. K., & Al-Ani, E. W. (2023). Optimizing media sterilization via chlorine dioxide and autoclaving of paulowni micropropagation. Iraqi Journal of Agricultural Sciences, 54(6), 1737-1745.
  • Al-Obaidy, O. M., & Khierallah, H. S. M. (2017). The roll of some plant growth regulators on shoots multiplication of stevia plants in vitro. The Iraqi Journal of Agricultural Science, 48(5), 1158-1168.
  • Al-sumaidai, K. M. I. (2017). Applications in plant biotechnologies. University administration for printing, publishing and translation. University of Nahrain. Iraq.
  • Alwash. B.M.J. and Jassim, A, Z. 2014. Study the role of auxins and sytoknins on in vitro propagation of Spilanthes acmella (L.) Murr. Baghdad science journal.vol .11 No.3.
  • Annon, A. H., & Abdulrasool, I. J. (2020). Effect of gamma radiation and ethyl methanesulfonate (ems) on potato salt stress tolerance in vitro. The Iraqi Journal of Agricultural Science, 51(4), 982-990.
  • Bani, S. H. S., Shaaban, L., & Al-Mizory, M. (2017). Effect of Media cultures and Benzyladenine (Ba) concentrations in micropropagation of three types of Cherry Rootstock (Establishment and Multiplication). The Iraqi Journal of Agricultural Science, 48, 113. https://doi.org/10.36103/ijas.v48iSpecial.252
  • Barhoumi, E. M., Charabi, Y., & Farhani, S. (2024). Detailed guide to machine learning techniques in signal processing. Progress in Electronics and Communication Engineering, 2(1), 39–47.
  • Chauhan, R. D., & Kanwar, K. (2012). Biotechnological advances in pomegranate (Punica granatum L.). In Vitro Cellular & Developmental Biology-Plant, 48, 579-594.
  • Desai, P., Patil, G., Dholiya, B., Desai, S., Patel, F., & Narayanan, S. (2018). Development of an efficient micropropagation protocol through axillary shoot proliferation for pomegranate variety ‘Bhagwa’. Annals of Agrarian science, 16(4), 444-450. https://doi.org/10.1016/j.aasci.2018.06.002
  • Fakhrian, M., Jafariyan, M., Pirali Zefrehei, A. R., & Sahraei, H. (2022). Effect of dietary medicinal plants on some biochemical hematological parameters of sterlet (Acipenser ruthenus). International Journal of Aquatic Research and Environmental Studies, 2(1), 55-59. https://doi.org/10.70102/IJARES/V2I1/6
  • Folta, K. M., Dhingra, A., Howard, L., Stewart, P. J., & Chandler, C. K. (2006). Characterization of LF9, an octoploid strawberry genotype selected for rapid regeneration and transformation. Planta, 224, 1058-1067.
  • Ghorbani, A., & Hatami, R. 2016. In vitro micropropagation of fig (Ficus carica L.) using nodal segments. Acta Agriculturae Slovenica, 108(2), 165-175.
  • Heisler, M. G., & Byrne, M. E. (2020). Progress in understanding the role of auxin in lateral organ development in plants. Current Opinion in Plant Biology, 53, 73-79. https://doi.org/10.1016/j.pbi.2019.10.007
  • Kaji, B. V., Ershadi, A., & Tohidfar, M. (2013). In vitro propagation of pomegranate (Punica granatum 1.) Cv.'Males Yazdi'. Albanian Journal of Agricultural Sciences, 12(1), 43.
  • Kanwar, K., Joseph, J., & Deepika, R. (2010). Comparison of in vitro regeneration pathways in Punica granatum L. Plant Cell, Tissue and Organ Culture (PCTOC), 100, 199-207.
  • Kinfe, B., Feyssa, T., & Bedada, G. (2017). In vitro micropropagation of grape vine (Vitis vinifera L.) from nodal culture. African Journal of Biotechnology, 16(43), 2083-2091. https://doi.org/10.5897/AJB2016.15803
  • Kurmi, U. S., Sharma, D. K., Tripathi, M. K., Tiwari, R., Baghel, B. S., & Tiwari, S. (2011). Plant regeneration of Vitis vinifera (L) via direct and indirect organogenesis from cultured nodal segments. Journal of Agricultural Technology, 7(3), 721-737.
  • Landi, L., & Mezzetti, B. (2006). TDZ, auxin and genotype effects on leaf organogenesis in Fragaria. Plant cell reports, 25, 281-288.
  • Lloyd, G and B. McCown. 1981.Commercially feasible micropropagation of Lu, M.C. 2005. Micropropagation of Vitis thunbergii Sieb. et Zucc., a medicinal herb,through high- frequency shoot tip culture. Sci.Hortic. 107: 64–69.
  • Lloyd, G., & McCown, B. H. (1980). Mountain laurel (Kalmia latifolia) by use of shoot tip culture. Proceedings of the International Plant Propagators' Society, 30, 421–427.
  • Majid, A. (2018). The Activity of Antioxidants Enzymes and NPK Contents as Affected by Water Quality, Kinetin, Bio and Organic Fertilization in Lettuce (Lactuca sativa L.). Iraqi Journal of Agricultural Sciences, 49(3).
  • Mozafari, A. A., Ghoraishi, O., Ghaderi, N., & Javadi, T. (2016). Micropropagation of grape cultivars (Vitis vinifera L.) on different basal media supplemented with benzyl adenine. Agriculturae Conspectus Scientificus, 81(3), 123-129.
  • Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia plantarum, 15(3). https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  • Osama, S. S. (2022). Micropropagation of grapevine (Vitis Vinifera L.) Cvs. red globe and Superior. The Iraqi Journal of Agricultural Science, 53(4), 833-849.
  • Pal, R. K., Babu, K. D., Singh, N. V., Ashis Maity, A. M., & Nilesh Gaikwad, N. G. (2014). Pomegranate research in India-status and future challenges.
  • Parab, A. R., Chew, B. L., Yeow, L. C., & Subramaniam, S. (2021). Organogenesis on apical buds in common fig (Ficus carica) var. Black Jack. Electronic Journal of Biotechnology, 54, 69-76. https://doi.org/10.1016/j.ejbt.2021.10.001
  • Passey, A., Barrett, K., & James, D. (2003). Adventitious shoot regeneration from seven commercial strawberry cultivars (Fragaria× ananassa Duch.) using a range of explant types. Plant cell reports, 21, 397-401.
  • Patil, V. M., Dhande, G. A., Thigale, D. M., & Rajput, J. C. (2011). Micropropagation of pomegranate (Punica granatum L.) ‘Bhagava’cultivar from nodal explant. African journal of Biotechnology, 10(79), 18130-18136. https://doi.org/10.5897/AJB11.1437
  • Prabhuling, Guranna, & Hoolageri Huchesh. "Direct in vitro regeneration in fig (Ficus carcia L.) cv.‘Brown Turkey’." Research Journal of Biotechnology 13.5 (2018): 77-83.
  • Rajabi, A., Atashi, S., & Khosh-Khui, M. (2018). Effects of thidiazuron, auxins, and explant type on in vitro rooting of fig (Ficus carica L.) plantlets. Journal of Plant Growth Regulation, 37(4), 1314-1324.
  • Rezaei et al. (2014) demonstrated the role of TDZ in somatic embryogenesis and plantlet regeneration in fig.
  • Ružić, D., Vujović, T., Libiakova, G., Cerović, R., & Gajdošova, A. (2012). Micropropagation in vitro of highbush blueberry (Vaccinium corymbosum L.). Journal of berry research, 2(2), 97-103. https://doi.org/10.3233/JBR-2012-030
  • Sarkhosh, A. Yavari, A. Ferguson. L. 2022. The Fig Botany, Production and https://doi.org/10.1079/9781789242881.0000
  • Seifi, E., Saeedipour, S., & Jalali-Javaran, M. (2016). Micropropagation of fig (Ficus carica L.) using single-node explants. Acta Agriculturae Slovenica, 107(1), 147-156.
  • Shahatha, E. F., Al-Mousawi, A. H., & Ibrahim, K. M. (2012). The Effect of different concentration of growth regulater on micropropagtion of Lisianthus (Eustoma grandiflora). Baghdad Science Journal, 9(3), 378-385.
  • Shi, S., Caluyo, F., Hernandez, R., Sarmiento, J., & Rosales, C. A. (2024). Automatic Classification and Identification of Plant Disease Identification by Using a Convolutional Neural Network. Natural and Engineering Sciences, 9(2), 184-197. https://doi.org/10.28978/nesciences.1569560
  • Sriskanda. D iew Y.X PingKhorF.M. Chew L. 2021. An efficient micropropagation protocol for Ficus carica cv. Golden Orphan suitable for mass propagation Biocatalysis and Agricultural Biotechnology Volume 38, November.
  • Toro, J. A. R., Sampietro-Saquicela, J. L., Suryasa, I. W., Solórzano, L. E. H., Cervantes, X. L. G., & Quiñónez, B. F. C. (2024). Optimal scheduling of renewable energy resources in energy management systems using hybrid genetic algorithm and particle swarm optimization. Acta Innovations, 52, 19-27.
  • Yildiz, M. (2012). The prerequisite of the success in plant tissue culture: high frequency shoot regeneration. In Recent advances in plant in vitro culture. IntechOpen. https://doi.org/10.5772/51097
There are 45 citations in total.

Details

Primary Language English
Subjects Agricultural Marine Biotechnology
Journal Section Articles
Authors

Ali Adil Abdulkareem 0000-0002-0227-7172

Ahmed Alsabte 0000-0001-8672-6088

Omar Ali Dahham 0009-0006-7883-4314

Samara Alzuhairi 0000-0003-3553-4515

Publication Date April 1, 2025
Submission Date March 19, 2025
Acceptance Date March 27, 2025
Published in Issue Year 2025 Volume: 10 Issue: 1

Cite

APA Abdulkareem, A. A., Alsabte, A., Dahham, O. A., Alzuhairi, S. (2025). Influence Different Concentrations of Thidiazurone (TDZ) on in Vitro Micropropagation of Two Cultivars of Ficus Carica L. Natural and Engineering Sciences, 10(1), 529-538. https://doi.org/10.28978/nesciences.1661006

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