Dual manyetik alan tedavisi, sıçanlarda LPS ile indüklenen pankreas hasarını, adacık yapısını ve endokrin fonksiyonu koruyarak azaltır
Abstract
Amaç: Sepsis, pankreas disfonksiyonu da dahil olmak üzere çoklu organ yetmezliğine yol açabilen, yaşamı tehdit eden sistemik bir inflamatuar yanıttır. Bu çalışmanın amacı, lipopolisakkarit (LPS) ile indüklenen pankreas hasarı üzerinde radyo frekans (RF) ve darbeli manyetik alan (PM) tedavilerinin koruyucu etkilerini değerlendirmektir.
Gereç ve yöntem: Kırk dişi Wistar albino rat Kontrol, LPS, LPS+RF, LPS+PM ve LPS+PM+RF olmak üzere rastgele beş gruba ayrıldı (n=8). LPS, intraperitoneal olarak (5 mg/kg) uygulandı, ardından RF (30 dakika) ve/veya PM (3 saat) uygulamaları yapıldı. Pankreas dokuları histopatolojik olarak incelendi ve immünohistokimyasal olarak insülin, glukagon ve amilin ekspresyonları değerlendirildi.
Bulgular: LPS uygulaması sonucunda pankreaslarda hiperemi, ödem ve inflamatuar infiltrasyon gibi belirgin histopatolojik hasarlar gözlendi. Ayrıca adacık hücrelerinde insülin ve amilin ekspresyonları azaldığı, glukagon ekspresyonu ise arttığı dikkati çekti. Hem RF hem de PM tedavilerinin bu değişiklikleri hafifletti saptandı. Özellikle PM+RF kombinasyonu, normal histolojik yapının ve hormonal expresyonların en belirgin şekilde düzeltilmesini sağladı.
Sonuç: RF ve PM tedavileri, özellikle birlikte uygulandıklarında, sepsise bağlı pankreas hasarına karşı önemli koruyucu etkiler göstermektedir. Bu etkiler, inflamasyonu azaltma ve adacık hücre fonksiyonlarını koruma yoluyla gerçekleşmektedir. Bulgular, inflamatuar durumlarda pankreas sağlığını desteklemek için farmakolojik olmayan yeni bir yaklaşımı ortaya koymaktadır.
Keywords
Ethical Statement
10/222 1/11/2023 tarihli karar Süleyman Demirel Üniversitesi Hayvan Deneyleri Yerel Etik Kurulu
Supporting Institution
Bu çalışma Süleyman Demirel Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından TSG-2024-9515 proje kodu ile desteklenmiştir.
Thanks
Yazarlar, destekleri için Süleyman Demirel Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi'ne ve doku örneklerini sağladığı için Dr. M. Soner Özcan'a teşekkür eder.
References
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- Li Y, Suo L, Zhang J. Zhonghua Wei Zhong Bing Ji Jiu Yi Xue. 2020;32(4):504-507. doi:10.3760/cma.j.cn121430-20191021-00063
- Virzì GM, Mattiotti M, de Cal M, Ronco C, Zanella M, De Rosa S. Endotoxin in Sepsis: Methods for LPS Detection and the Use of Omics Techniques. Diagnostics (Basel). 2022;13(1):79. doi:10.3390/diagnostics13010079
- Yang L, Zhou R, Tong Y, et al. Neuroprotection by dihydrotestosterone in LPS-induced neuroinflammation. Neurobiol Dis. 2020;140:104814. doi:10.1016/j.nbd.2020.104814
- Campbell JE, Newgard CB. Mechanisms controlling pancreatic islet cell function in insulin secretion. Nat Rev Mol Cell Biol. 2021;22(2):142-158. doi:10.1038/s41580-020-00317-7
- Mehdi SF, Qureshi MH, Pervaiz S, et al. Endocrine and metabolic alterations in response to systemic inflammation and sepsis: a review article. Mol Med. 2025;31(1):16. doi:10.1186/s10020-025-01074-z
- Kahles F, Meyer C, Möllmann J, et al. GLP-1 secretion is increased by inflammatory stimuli in an IL-6-dependent manner, leading to hyperinsulinemia and blood glucose lowering. Diabetes. 2014;63(10):3221-3229. doi:10.2337/db14-0100
- Morikawa S, Kaneko N, Okumura C, et al. IAPP/amylin deposition, which is correlated with expressions of ASC and IL-1β in β-cells of Langerhans' islets, directly initiates NLRP3 inflammasome activation. Int J Immunopathol Pharmacol. 2018;32:2058738418788749. doi:10.1177/2058738418788749
- Asci H, Savran M, Comlekci S, et al. Combined Pulsed Magnetic Field and Radiofrequency Electromagnetic Field Enhances MMP-9, Collagen-4, VEGF Synthesis to Improve Wound Healing Via Hif-1α/eNOS Pathway. Aesthetic Plast Surg. 2023;47(6):2841-2852. doi:10.1007/s00266-023-03450-8
- Ma T, Ding Q, Liu C, Wu H. Electromagnetic fields regulate calcium-mediated cell fate of stem cells: osteogenesis, chondrogenesis and apoptosis. Stem Cell Res Ther. 2023;14(1):133. doi:10.1186/s13287-023-03303-w
- Gunay I, Baldan I, Tokus M, Coskun C, Ocal I, Cicek FA. Pulsed magnetic field maintains vascular homeostasis against H2O2-induced oxidative stress. Gen Physiol Biophys. 2020;39(6):579-586. doi:10.4149/gpb_2020035
- Zhou S, Wen H, He X, Han X, Li H. Pulsed electromagnetic field ameliorates the progression of osteoarthritis via the Sirt1/NF-κB pathway. Arthritis Res Ther. 2025;27(1):33. doi:10.1186/s13075-025-03492-0
- Balci Ç, Özcan MS, Aşci H, et al. Radiofrequency Electromagnetic and Pulsed Magnetic Fields Protected the Kidney Against Lipopolysaccharide-Induced Acute Systemic Inflammation, Oxidative Stress, and Apoptosis by Regulating the IL-6/HIF1α/eNOS and Bcl2/Bax/Cas-9 Pathways. Medicina (Kaunas). 2025;61(2):238. doi:10.3390/medicina61020238
- Wang G, Zhao X, He G, Li Z, Wang G, Lv Y. Effect of Butyric Acid on Apoptosis of Pancreatic β Cells. Ann Clin Lab Sci. 2025;55(2):259-267.
- Ozmen O, Topsakal S. Preventive effect of agomelatine in lipopolysaccharide-induced pancreatic pathology. Drug Chem Toxicol. 2022;45(1):180-184. doi:10.1080/01480545.2019.1675686
- Topsakal S, Ozmen O, Karakuyu NF, Bedir M, Sancer O. Cannabidiol Mitigates Lipopolysaccharide-Induced Pancreatic Pathology: A Promising Therapeutic Strategy. Cannabis Cannabinoid Res. 2024;9(3):809-818. doi:10.1089/can.2023.0153
- Mukai E, Fujimoto S, Inagaki N. Role of Reactive Oxygen Species in Glucose Metabolism Disorder in Diabetic Pancreatic β-Cells. Biomolecules. 2022;12(9):1228. doi:10.3390/biom12091228
- Miki A, Ricordi C, Sakuma Y, et al. Divergent antioxidant capacity of human islet cell subsets: A potential cause of beta-cell vulnerability in diabetes and islet transplantation. PLoS One. 2018;13(5):e0196570. doi:10.1371/journal.pone.0196570
- Gualdi G, Costantini E, Reale M, Amerio P. Wound Repair and Extremely Low Frequency-Electromagnetic Field: Insight from In Vitro Study and Potential Clinical Application. Int J Mol Sci. 2021;22(9):5037. doi:10.3390/ijms22095037
- Hernández Morales M, Shang T, Chen J, Han V, Liu C. Lipid Oxidation Induced by RF Waves and Mediated by Ferritin Iron Causes Activation of Ferritin-Tagged Ion Channels. Cell Rep. 2020;30(10):3250-3260.e7. doi:10.1016/j.celrep.2020.02.070
- Jimenez H, Wang M, Zimmerman JW, et al. Tumour-specific amplitude-modulated radiofrequency electromagnetic fields induce differentiation of hepatocellular carcinoma via targeting Cav3.2 T-type voltage-gated calcium channels and Ca2+ influx. EBioMedicine. 2019;44:209-224. doi:10.1016/j.ebiom.2019.05.034
- Jia Q, Tian F, Duan WN, Jia YF, Wang HX, Xia ZY. Effects of Dezocine-Remifentanil Intravenous Anaesthesia on Perioperative Signs, Serum TNF-&aipha; and IL-6 in Liver Cancer Patients undergoing Radiofrequency Ablation. J Coll Physicians Surg Pak. 2019;29(1):4-7. doi:10.29271/jcpsp.2019.01.4
- Zheng Y, Huang Q, Zhang Y, et al. Multimodal roles of transient receptor potential channel activation in inducing pathological tissue scarification. Front Immunol. 2023;14:1237992. doi:10.3389/fimmu.2023.1237992
- Zhao M, Liu A, Wu J, Mo L, Lu F, Wan G. Il1r2 and Tnfrsf12a in transcranial magnetic stimulation effect of ischemic stroke via bioinformatics analysis. Medicine (Baltimore). 2024;103(4):e36109. doi:10.1097/MD.0000000000036109
- Falone S, Santini S Jr, Cordone V, et al. Extremely Low-Frequency Magnetic Fields and Redox-Responsive Pathways Linked to Cancer Drug Resistance: Insights from Co-Exposure-Based In Vitro Studies. Front Public Health. 2018;6:33. doi:10.3389/fpubh.2018.00033
- Andreozzi F, Di Fatta C, Spiga R, et al. Glucagon induces the hepatic expression of inflammatory markers in vitro and in vivo. Diabetes Obes Metab. 2023;25(2):556-569. doi:10.1111/dom.14902
- Hou X, Sun L, Li Z, et al. Associations of amylin with inflammatory markers and metabolic syndrome in apparently healthy Chinese. PLoS One. 2011;6(9):e24815. doi:10.1371/journal.pone.0024815
- Mehdi SF, Qureshi MH, Pervaiz S, et al. Endocrine and metabolic alterations in response to systemic inflammation and sepsis: a review article. Mol Med. 2025;31(1):16. doi:10.1186/s10020-025-01074-z
- Chen Q, Chen X, Jia Z, et al. ∆nFGF1 Protects β-Cells against High Glucose-Induced Apoptosis via the AMPK/SIRT1/PGC-1α Axis. Oxid Med Cell Longev. 2022;2022:1231970. doi:10.1155/2022/1231970
- Hu G, Malik AB, Minshall RD. Toll-like receptor 4 mediates neutrophil sequestration and lung injury induced by endotoxin and hyperinflation. Crit Care Med. 2010;38(1):194-201. doi:10.1097/CCM.0b013e3181bc7c17
Dual magnetic field therapy mitigates LPS-induced pancreatic injury by preserving islet structure and endocrine function in rats
Abstract
Purpose: Sepsis is a life-threatening systemic inflammatory response that can lead to multiorgan failure, including pancreatic dysfunction. This study aimed to investigate the potential protective effects of radiofrequency (RF) and pulsed magnetic field (PM) therapies against lipopolysaccharide (LPS)-induced pancreatic injury in rats.
Materials and methods: Forty female Wistar albino rats were randomly assigned to five groups (n=8 per group): Control, LPS, LPS + RF, LPS + PM, and LPS + PM + RF. LPS was administered intraperitoneally (5 mg/kg), followed by RF (30 minutes) and/or PM (3 hours) exposure. Pancreatic tissues were examined histopathologically and immunohistochemically for insulin, glucagon, and amylin expression.
Results: LPS administration resulted in significant histopathological damage, including hyperemia, edema, and inflammatory infiltration, as well as reduced insulin and amylin immunopositivity and increased glucagon expression in islets. Both RF and PM treatments alleviated these changes, with the combined PM+RF application showing the most prominent protective effects, restoring normal histological architecture and hormonal profiles.
Conclusion: RF and PM therapies, particularly in combination, exhibit significant protective effects against sepsis-induced pancreatic injury by mitigating inflammation and preserving islet cell function. These findings highlight a novel non-pharmacological approach for supporting pancreatic health in inflammatory conditions.
Keywords
Ethical Statement
10/222 decision dated 1/11/2023 Süleyman Demirel University Animal Experiments Local Ethics Committee
Supporting Institution
The authors disclosed receipt of the following financial support for the research. This study was supported by the Scientific Research Projects Coordination Unit of Süleyman Demirel University with project code TSG-2024-9515.
Thanks
The authors would like to thank the Scientific Research Projects Coordination Unit of Süleyman Demirel University for their support and Dr. M. Soner Özcan for providing the tissue samples.
References
- Spapen H. Liver perfusion in sepsis, septic shock, and multiorgan failure. Anat Rec (Hoboken). 2008;291(6):714-720. doi:10.1002/ar.20646
- Li Y, Suo L, Zhang J. Zhonghua Wei Zhong Bing Ji Jiu Yi Xue. 2020;32(4):504-507. doi:10.3760/cma.j.cn121430-20191021-00063
- Virzì GM, Mattiotti M, de Cal M, Ronco C, Zanella M, De Rosa S. Endotoxin in Sepsis: Methods for LPS Detection and the Use of Omics Techniques. Diagnostics (Basel). 2022;13(1):79. doi:10.3390/diagnostics13010079
- Yang L, Zhou R, Tong Y, et al. Neuroprotection by dihydrotestosterone in LPS-induced neuroinflammation. Neurobiol Dis. 2020;140:104814. doi:10.1016/j.nbd.2020.104814
- Campbell JE, Newgard CB. Mechanisms controlling pancreatic islet cell function in insulin secretion. Nat Rev Mol Cell Biol. 2021;22(2):142-158. doi:10.1038/s41580-020-00317-7
- Mehdi SF, Qureshi MH, Pervaiz S, et al. Endocrine and metabolic alterations in response to systemic inflammation and sepsis: a review article. Mol Med. 2025;31(1):16. doi:10.1186/s10020-025-01074-z
- Kahles F, Meyer C, Möllmann J, et al. GLP-1 secretion is increased by inflammatory stimuli in an IL-6-dependent manner, leading to hyperinsulinemia and blood glucose lowering. Diabetes. 2014;63(10):3221-3229. doi:10.2337/db14-0100
- Morikawa S, Kaneko N, Okumura C, et al. IAPP/amylin deposition, which is correlated with expressions of ASC and IL-1β in β-cells of Langerhans' islets, directly initiates NLRP3 inflammasome activation. Int J Immunopathol Pharmacol. 2018;32:2058738418788749. doi:10.1177/2058738418788749
- Asci H, Savran M, Comlekci S, et al. Combined Pulsed Magnetic Field and Radiofrequency Electromagnetic Field Enhances MMP-9, Collagen-4, VEGF Synthesis to Improve Wound Healing Via Hif-1α/eNOS Pathway. Aesthetic Plast Surg. 2023;47(6):2841-2852. doi:10.1007/s00266-023-03450-8
- Ma T, Ding Q, Liu C, Wu H. Electromagnetic fields regulate calcium-mediated cell fate of stem cells: osteogenesis, chondrogenesis and apoptosis. Stem Cell Res Ther. 2023;14(1):133. doi:10.1186/s13287-023-03303-w
- Gunay I, Baldan I, Tokus M, Coskun C, Ocal I, Cicek FA. Pulsed magnetic field maintains vascular homeostasis against H2O2-induced oxidative stress. Gen Physiol Biophys. 2020;39(6):579-586. doi:10.4149/gpb_2020035
- Zhou S, Wen H, He X, Han X, Li H. Pulsed electromagnetic field ameliorates the progression of osteoarthritis via the Sirt1/NF-κB pathway. Arthritis Res Ther. 2025;27(1):33. doi:10.1186/s13075-025-03492-0
- Balci Ç, Özcan MS, Aşci H, et al. Radiofrequency Electromagnetic and Pulsed Magnetic Fields Protected the Kidney Against Lipopolysaccharide-Induced Acute Systemic Inflammation, Oxidative Stress, and Apoptosis by Regulating the IL-6/HIF1α/eNOS and Bcl2/Bax/Cas-9 Pathways. Medicina (Kaunas). 2025;61(2):238. doi:10.3390/medicina61020238
- Wang G, Zhao X, He G, Li Z, Wang G, Lv Y. Effect of Butyric Acid on Apoptosis of Pancreatic β Cells. Ann Clin Lab Sci. 2025;55(2):259-267.
- Ozmen O, Topsakal S. Preventive effect of agomelatine in lipopolysaccharide-induced pancreatic pathology. Drug Chem Toxicol. 2022;45(1):180-184. doi:10.1080/01480545.2019.1675686
- Topsakal S, Ozmen O, Karakuyu NF, Bedir M, Sancer O. Cannabidiol Mitigates Lipopolysaccharide-Induced Pancreatic Pathology: A Promising Therapeutic Strategy. Cannabis Cannabinoid Res. 2024;9(3):809-818. doi:10.1089/can.2023.0153
- Mukai E, Fujimoto S, Inagaki N. Role of Reactive Oxygen Species in Glucose Metabolism Disorder in Diabetic Pancreatic β-Cells. Biomolecules. 2022;12(9):1228. doi:10.3390/biom12091228
- Miki A, Ricordi C, Sakuma Y, et al. Divergent antioxidant capacity of human islet cell subsets: A potential cause of beta-cell vulnerability in diabetes and islet transplantation. PLoS One. 2018;13(5):e0196570. doi:10.1371/journal.pone.0196570
- Gualdi G, Costantini E, Reale M, Amerio P. Wound Repair and Extremely Low Frequency-Electromagnetic Field: Insight from In Vitro Study and Potential Clinical Application. Int J Mol Sci. 2021;22(9):5037. doi:10.3390/ijms22095037
- Hernández Morales M, Shang T, Chen J, Han V, Liu C. Lipid Oxidation Induced by RF Waves and Mediated by Ferritin Iron Causes Activation of Ferritin-Tagged Ion Channels. Cell Rep. 2020;30(10):3250-3260.e7. doi:10.1016/j.celrep.2020.02.070
- Jimenez H, Wang M, Zimmerman JW, et al. Tumour-specific amplitude-modulated radiofrequency electromagnetic fields induce differentiation of hepatocellular carcinoma via targeting Cav3.2 T-type voltage-gated calcium channels and Ca2+ influx. EBioMedicine. 2019;44:209-224. doi:10.1016/j.ebiom.2019.05.034
- Jia Q, Tian F, Duan WN, Jia YF, Wang HX, Xia ZY. Effects of Dezocine-Remifentanil Intravenous Anaesthesia on Perioperative Signs, Serum TNF-&aipha; and IL-6 in Liver Cancer Patients undergoing Radiofrequency Ablation. J Coll Physicians Surg Pak. 2019;29(1):4-7. doi:10.29271/jcpsp.2019.01.4
- Zheng Y, Huang Q, Zhang Y, et al. Multimodal roles of transient receptor potential channel activation in inducing pathological tissue scarification. Front Immunol. 2023;14:1237992. doi:10.3389/fimmu.2023.1237992
- Zhao M, Liu A, Wu J, Mo L, Lu F, Wan G. Il1r2 and Tnfrsf12a in transcranial magnetic stimulation effect of ischemic stroke via bioinformatics analysis. Medicine (Baltimore). 2024;103(4):e36109. doi:10.1097/MD.0000000000036109
- Falone S, Santini S Jr, Cordone V, et al. Extremely Low-Frequency Magnetic Fields and Redox-Responsive Pathways Linked to Cancer Drug Resistance: Insights from Co-Exposure-Based In Vitro Studies. Front Public Health. 2018;6:33. doi:10.3389/fpubh.2018.00033
- Andreozzi F, Di Fatta C, Spiga R, et al. Glucagon induces the hepatic expression of inflammatory markers in vitro and in vivo. Diabetes Obes Metab. 2023;25(2):556-569. doi:10.1111/dom.14902
- Hou X, Sun L, Li Z, et al. Associations of amylin with inflammatory markers and metabolic syndrome in apparently healthy Chinese. PLoS One. 2011;6(9):e24815. doi:10.1371/journal.pone.0024815
- Mehdi SF, Qureshi MH, Pervaiz S, et al. Endocrine and metabolic alterations in response to systemic inflammation and sepsis: a review article. Mol Med. 2025;31(1):16. doi:10.1186/s10020-025-01074-z
- Chen Q, Chen X, Jia Z, et al. ∆nFGF1 Protects β-Cells against High Glucose-Induced Apoptosis via the AMPK/SIRT1/PGC-1α Axis. Oxid Med Cell Longev. 2022;2022:1231970. doi:10.1155/2022/1231970
- Hu G, Malik AB, Minshall RD. Toll-like receptor 4 mediates neutrophil sequestration and lung injury induced by endotoxin and hyperinflation. Crit Care Med. 2010;38(1):194-201. doi:10.1097/CCM.0b013e3181bc7c17
Details
| Primary Language | English |
|---|---|
| Subjects | Endocrinology |
| Journal Section | Research Article |
| Authors | |
| Early Pub Date | July 29, 2025 |
| Publication Date | |
| Submission Date | June 18, 2025 |
| Acceptance Date | July 29, 2025 |
| Published in Issue | Year 2025 Volume: 18 Issue: 4 |
