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Smooth Muscle Cells are Derived Predominantly from Tissue Explant of Inguinal Hernia Sac

Year 2018, Volume: 16 Issue: 1, 29 - 34, 01.04.2018

Adnan Narcı Mevlit Korkmaz Tahsin Yakut Burcu Biltekin Ayhan Bilir Feridun Cahit Tanyel

Abstract

GİRİŞ ve AMAÇ: Processus vaginalisin (PV) kapanmasının, testisin inişini sağlamak için geçici olarak
bulunan düz kas hücrelerinin, devamlılığına bağlı olduğu ileri sürülmüştür. Bu amaçla, inguinal herni
keseleri, PV kapanması ve düz kas hücre varlığı arasındaki ilişkinin değerlendirilmesi için kültüre edildi.
Bu çalışma, sadece inguinal herni keselerinde düz kas hücresi varlığını gösteren ek bilgiler vermekle
kalmayıp, aynı zamanda inguinal herninin cerrahi dışı tedavisi için yapılacak çalışmalar için yeni fikirler
sunmaktadır.

YÖNTEM ve GEREÇLER: Herni keseleri, yaşları 2 ay ile 5 yaş arasında değişen 11 çocuktan inguinal
herni ameliyatı sırasında elde edildi. Örnekler uygun şekilde hazırlandı ve kültüre edildi. Hücrelerin
morfolojik özellikleri ışık mikroskobu ile değerlendirildi. Hücrelerin hayatiyetleri, tripan blue exclusion
metodu ile değerlendirildi. Gelişen hücreler, imminohistokimyasal olarak aktin ve miyozin ile boyandı.

BULGULAR: Işık mikroskobu incelemesi ile bu hücrelerin iğ şekilli olduğu ve santral yerleşimli
yuvarlak çekirdeklerinin bulunduğu görülmüştür. Tüm flask üreyen hücrelerle dolduğunda, kontakt
inhibisyon olmadığından, üst üste çoğalan hücreler tipik tepe-vadi görünümü oluşturmuştur. Hücrelerin
canlılığı %95’ in üzerinde bulunmuştur. Gelişen hücrelerin % 80 inin düz kas aktin ve düz kas myosin
antikorları ile boyandığı saptanmıştır.

TARTIŞMA ve SONUÇ: Herni kesesi dokulardan gelişen hücrelerin büyük çoğunluğunu, düz kas
hücreleri oluşturmuştur. Bu bulgu, PV’in inhibisyonu ile düz kas hücresi varlığı arasındaki ilişkiyi
desteklemektedir. Bu bilgi inguinal herninin cerrahi dışı tedavisi üzerinde yapılacak çalışmalar için
kullanılabilecektir.

ABSTRACT

INTRODUCTION: Obliteration of processus vaginalis (PV) has been proposed to result from
persistence of smooth muscle which is presented transiently to propel the testis. Sacs associated with
inguinal hernia were cultivated to define the cells that are going to proliferate for evaluating the
association of inhibition of obliteration of PV and the presence of smooth muscle (SM). The present
study does not only provide additional information about the presence of SM in sacs from boys with
inguinal hernia, but also provides a new tool for researches directed to define the non- operative
treatment of inguinal hernia.

METHODS: Hernia sacs were obtained from eleven boys with the ages ranging from two months to
five years during operations for inguinal hernia. Samples were prepared and cultivated. Morphologic
characteristics of cell populations were examined by light microscopy. Viability was estimated by trypan
blue exclusion method. Growing cells were identified via immunohistochemical staining for smooth
muscle actin and myosin.

RESULTS: Light microscopic images of growing cells displayed characteristic spindle shaped
morphology with centrally located round nucleus. When the flasks reached confluence, a hill-valley
appearance was observed because of absence of contact inhibition. Cell viability was found more than
95%. Approximately, 80% of growing cell populations was stained positive with actins and myosin
antibodies.
DISCUSSION and

CONCLUSION: In tissue explants of hernia sac, most commonly proliferating cell
type is smooth muscle cells. This evidence supports the association of inhibition of PV and the presence
of SM. The SM obtained from sacs associated with inguinal hernia may be used for researches directed
to establish the non-operative treatment of inguinal hernia. 

Keywords

inguinal hernia sac smooth muscle cell tissue culture

References

  • 1. Cox JA: Inguinal hernia of childhood. Surg Clin North 1985; 65:1331-42.
  • 2. Tanyel FC, Talim B, Atilla P, et al: Myogenesis within the human gubernaculum: histological and immunohistological evaluation. European Journal of Pediatric Surgery 2005; 15: 175-9.
  • 3. Heyns CF. The gubernaculum during testicular descent in the human fetus. J Anat 987; 153: 93e112.
  • 4. Backhouse KM. The natural history of testicular descent and maldescent. Proc R Soc Med 1966; 59: 357e60.
  • 5. Shrock P. The processus vaginalis and gubernaculum. Their raison d’etre redefined. Surg Clin North Am 1971; 51:1263e8.
  • 6. Tanyel FC, Dağdeviren A, Müftüoğlu S, et al: Inguinal hernia revisited through comparative evaluation of peritoneum, processus vaginalis, and sacs obtained from children with hernia, hydrocele, and undescended testis. J Pediatr Surg 1999;34:552-5.
  • 7. Tanyel FC, Ulusu N, Tezcan EF, et al: Total calcium content of sacs associated with inguinal hernia, hydrocele or undescended testis reflects differences dictated by programmed cell death. Urologia Internationalis 2003; 70: 211-5

Year 2018, Volume: 16 Issue: 1, 29 - 34, 01.04.2018

Adnan Narcı Mevlit Korkmaz Tahsin Yakut Burcu Biltekin Ayhan Bilir Feridun Cahit Tanyel

Abstract

References

  • 1. Cox JA: Inguinal hernia of childhood. Surg Clin North 1985; 65:1331-42.
  • 2. Tanyel FC, Talim B, Atilla P, et al: Myogenesis within the human gubernaculum: histological and immunohistological evaluation. European Journal of Pediatric Surgery 2005; 15: 175-9.
  • 3. Heyns CF. The gubernaculum during testicular descent in the human fetus. J Anat 987; 153: 93e112.
  • 4. Backhouse KM. The natural history of testicular descent and maldescent. Proc R Soc Med 1966; 59: 357e60.
  • 5. Shrock P. The processus vaginalis and gubernaculum. Their raison d’etre redefined. Surg Clin North Am 1971; 51:1263e8.
  • 6. Tanyel FC, Dağdeviren A, Müftüoğlu S, et al: Inguinal hernia revisited through comparative evaluation of peritoneum, processus vaginalis, and sacs obtained from children with hernia, hydrocele, and undescended testis. J Pediatr Surg 1999;34:552-5.
  • 7. Tanyel FC, Ulusu N, Tezcan EF, et al: Total calcium content of sacs associated with inguinal hernia, hydrocele or undescended testis reflects differences dictated by programmed cell death. Urologia Internationalis 2003; 70: 211-5
There are 7 citations in total.

Details

Primary Language English
Journal Section Araştırma
Authors

Adnan Narcı

Mevlit Korkmaz

Tahsin Yakut

Burcu Biltekin

Ayhan Bilir

Feridun Cahit Tanyel

Publication Date April 1, 2018
Published in Issue Year 2018 Volume: 16 Issue: 1

Cite

APA Narcı, A., Korkmaz, M., Yakut, T., Biltekin, B., et al. (2018). Smooth Muscle Cells are Derived Predominantly from Tissue Explant of Inguinal Hernia Sac. Güncel Pediatri, 16(1), 29-34.
AMA Narcı A, Korkmaz M, Yakut T, Biltekin B, Bilir A, Tanyel FC. Smooth Muscle Cells are Derived Predominantly from Tissue Explant of Inguinal Hernia Sac. Güncel Pediatri. April 2018;16(1):29-34.
Chicago Narcı, Adnan, Mevlit Korkmaz, Tahsin Yakut, Burcu Biltekin, Ayhan Bilir, and Feridun Cahit Tanyel. “Smooth Muscle Cells Are Derived Predominantly from Tissue Explant of Inguinal Hernia Sac”. Güncel Pediatri 16, no. 1 (April 2018): 29-34.
EndNote Narcı A, Korkmaz M, Yakut T, Biltekin B, Bilir A, Tanyel FC (April 1, 2018) Smooth Muscle Cells are Derived Predominantly from Tissue Explant of Inguinal Hernia Sac. Güncel Pediatri 16 1 29–34.
IEEE A. Narcı, M. Korkmaz, T. Yakut, B. Biltekin, A. Bilir, and F. C. Tanyel, “Smooth Muscle Cells are Derived Predominantly from Tissue Explant of Inguinal Hernia Sac”, Güncel Pediatri, vol. 16, no. 1, pp. 29–34, 2018.
ISNAD Narcı, Adnan et al. “Smooth Muscle Cells Are Derived Predominantly from Tissue Explant of Inguinal Hernia Sac”. Güncel Pediatri 16/1 (April 2018), 29-34.
JAMA Narcı A, Korkmaz M, Yakut T, Biltekin B, Bilir A, Tanyel FC. Smooth Muscle Cells are Derived Predominantly from Tissue Explant of Inguinal Hernia Sac. Güncel Pediatri. 2018;16:29–34.
MLA Narcı, Adnan et al. “Smooth Muscle Cells Are Derived Predominantly from Tissue Explant of Inguinal Hernia Sac”. Güncel Pediatri, vol. 16, no. 1, 2018, pp. 29-34.
Vancouver Narcı A, Korkmaz M, Yakut T, Biltekin B, Bilir A, Tanyel FC. Smooth Muscle Cells are Derived Predominantly from Tissue Explant of Inguinal Hernia Sac. Güncel Pediatri. 2018;16(1):29-34.