Research Article
Year 2025,
, 10 - 16, 28.03.2025
Abstract
Objective: In this study, our aim was to evaluate the relationship between COM, pyroptosis, and oxidative stress.
Materials and Methods: This prospective study included 60 patients who underwent surgery for chronic otitis media (COM) and 28 patients as a control group. Pathological tissue was taken from the middle ear in the study group, whereas healthy tissue was taken from the middle ear of patients who underwent cochlear implant surgery and had no signs of infection in the middle ear in the control group. Preoperative serum samples were obtained from all patients. Pyroptosis parameters, Nod-Like Receptor Protein 3 (NLRP3) and Interleukin-1 beta (IL-1β) were investigated. The oxidative stress parameters, including the total antioxidant status (TAS), Total Oxidant Status (TOS), and Oxidative Stress Index (OSI), were also examined.
Results: Of the 88 patients included in the study, 52 were men and 36 were women. The patients’ ages ranged from 18 to 59 years, with a mean of 31.07±11.45years. In our study, there was a significant difference in NLRP3, IL-1β, TAS, TOS and OSI values in all case groups compared with the control group in both tissue and serum samples (p<0.001).
Conclusion: The significant variation in NLRP3, IL-1β, TAS, TOS and OSI values in tissue and serum compared with the control group indicates that pyroptosis and oxidative stress may play an important role in the presence of COM and cholesteatoma. Understanding the role of pyroptosis in the developmental stages of COM is very important, and elucidation of this relationship may lead to the development of a new treatment method.
References
- Meyerhoff WL, Kim CS, Paparella MM. Pathology of chronic otitis media. Ann Otol Rhinol Laryngol Suppl 1978;87(6):749-60. google scholar
- Gopen Q. Fundamental Otology: Pediatric & Adult Practice: JP Medical Ltd; 2012. google scholar
- Dayasena R, Dayasiri M, Jayasuriya C, Perera D. Aetiological agents in chronic suppurative otitis media in Sri Lanka. Med J Aust 2011;4(2):101. google scholar
- Vanneste P, Page C. Otitis media with effusion in children: Pathophysiology, diagnosis, and treatment. A review. J Otol 2019;14(2):33-9. google scholar
- Lamkanfi M, Dixit VM. Mechanisms and functions of inflammasomes. Cell 2014;157(5):1013-22. google scholar
- Zaki MH, Lamkanfi M, Kanneganti T-D. The Nlrp3 inflammasome: contributions to intestinal homeostasis. Trends Immunol 2011;32(4):171-9. google scholar
- Kariya S, Okano M, Zhao P, Maeda Y, Kataoka Y, Higaki T, et al. NLRP3 inflammasome expression in lipopolysaccharide-induced otitis media. Acta Otolaryngol 2018;138(12):1061-5. google scholar
- Chien H, Dix RD. Evidence for multiple cell death pathways during development of experimental cytomegalovirus retinitis in mice with retrovirus-induced immunosuppression: apoptosis, necroptosis, and pyroptosis. J Virol 2012;86(20):10961-78. google scholar
- Taysi S, Memisogullari R, Koc M, Yazici AT, Aslankurt M, Gumustekin K, et al. Melatonin reduces oxidative stress in the rat lens due to radiation-induced oxidative injury. Int J Radiat Biol2008;84(10):803-8. google scholar
- Baysal E, Aksoy N, Kara F, Taysi S, Taşkın A, Bilinç H, et al. Oxidative stress in chronic otitis media. Eur Arch Otorhinolaryngol Suppl 2013;270:1203-8. google scholar
- Öktemer T, Kemaloğlu Y, Özbilen S, Tutar H. Kronik Otitis Media Cerrahisinde Greft Başarisini Etkileyen Faktörler. Osmangazi Tıp Dergisi 2016;38(3):46-54. google scholar
- Ford AH, Hankey GJ, Yeap BB, Golledge J, Flicker L, Almeida OP. Hearing loss and the risk of dementia in later life. Maturitas 2018;112:1-11. google scholar
- Karlı R, Karli A, Aksoy A, Açikgöz S. Orta kulak efüzyonlarında timpanogram ile otoskopik bulguların karşılaştırılması. Dicle Univ Tip Fakul Derg 2013;40(1):54-6. google scholar
- Xu Y-J, Zheng L, Hu Y-W, Wang Q. Pyroptosis and its relationship to atherosclerosis. Clin Chim Acta 2018;476:28-37. google scholar
- Martinon F, Burns K, Tschopp J. The inflammasome: a molecular platform triggering activation of inflammatory caspases and processing of proIL-β. Mol cell 2002;10(2):417-26. google scholar
- Man SM, Karki R, Kanneganti TD. Molecular mechanisms and functions of pyroptosis, inflammatory caspases and inflammasomes in infectious diseases. Immunol Rev 2017;277(1):61-75. google scholar
- Garça MF, Turan M, Avşar B, Kalkan F, Demir H, Kozan A, et al. The evaluation of oxidative stress in the serum and tissue specimens of patients with chronic otitis media. Clin Exp Otorhinolaryngol 2015;8(2):97-101. google scholar
- Halliwell B. Drug antioxidant effects: A basis for drug selection? Drugs 1991;42:569-605. google scholar
- Kariya S, Okano M, Zhao P, Kataoka Y, Yoshinobu J, Maeda Y, et al. Activation of NLRP3 inflammasome in human middle ear cholesteatoma and chronic otitis media. Acta Otolaryngol 2016;136(2):136-40. google scholar
- Karlıdağ T, İlhan N, Kaygusuz İ, Keleş E, Yalçn Ş. Comparison of free radicals and antioxidant enzymes in chronic otitis media with and without tympanosclerosis. Laryngoscope 2004;114(1):85-9. google scholar
- García Callejo F, Estors Ferrero J, Morant Ventura A, Segarra Cortés P, Velert Vila M. Lipoperoxidación en otorreas del oído medio como marcador de infección. Aplicación clínica. [Lipoperoxidation in otorrhea of the middle ear as a marker of infection. Clinical application]. Acta Otorrinolaringol Esp 2000;51(6):478-81. google scholar
- Aslan M, Çiçek MT. MPV ve NLO Değerleri kolesteatomu, kronik otitis mediadan ayırt eder mi? Van Tip Dergisi 2021;28(4):615-22. google scholar
- Macias MP, Gerkin RD, Macias JD. Increased amphiregulin expression as a biomarker of cholesteatoma activity. Laryngoscope 2010;120(11):2258-63. google scholar
- Lin H, Li Z, Lin D, Zheng C, Zhang W. Role of NLRP3 inflammasome in eosinophilic and non-eosinophilic chronic rhinosinusitis with nasal polyps. Inflammation 2016;39:2045-52. google scholar
- Wei Y, Zhang J, Wu X, Sun W, Wei F, Liu W, et al. Activated pyrin domain containing 3 (NLRP3) inflammasome in neutrophilic chronic rhinosinusitis with nasal polyps (CRSwNP). J Allergy Clin Immunol 2020;145(3):1002-5. google scholar
- Månsson A, Bogefors J, Cervin A, Uddman R, Cardell L-O. NOD‐like receptors in the human upper airways: a potential role in nasal polyposis. Allergy 2011;66(5):621-8. google scholar
- Kartlaşmış K, Dikmen N. Piroptozise biyokimyasal yaklaşım ve kanserdeki rolü. Arşiv Kaynak Tarama Dergisi 2020;29(3):181-9. google scholar
- Zhou R, Tardivel A, Thorens B, Choi I, Tschopp J. Thioredoxin-interacting protein links oxidative stress to inflammasome activation. Nat Immunol 2010;11(2):136-40. google scholar
- Salminen A, Ojala J, Kaarniranta K, Kauppinen A. Mitochondrial dysfunction and oxidative stress activate inflammasomes: impact on the aging process and age-related diseases. Cell Mol Life Sci 2012;69:2999-3013. google scholar
- Heid ME, Keyel PA, Kamga C, Shiva S, Watkins SC, Salter RD. Mitochondrial reactive oxygen species ınduces NLRP3-Dependent lysosomal damage and ınflammasome activation. J Immunol 2013;191(10):5230-8. google scholar
- Wu X, Zhang H, Qi W, Zhang Y, Li J, Li Z, et al. Nicotine promotes atherosclerosis via ROS-NLRP3-mediated endothelial cell pyroptosis. Cell Death Dis 2018;9(2):171. google scholar
- Nakahira K, Haspel JA, Rathinam VAK, Lee S-J, Dolinay T, Lam HC, et al. Autophagy proteins regulate innate immune responses by inhibiting the release of mitochondrial DNA mediated by the NALP3 inflammasome. Nat Immunol 2011;12(3):222-30. google scholar
- Hu Y, Mao K, Zeng Y, Chen S, Tao Z, Yang C, et al. Tripartite-motif protein 30 negatively regulates nlrp3 ınflammasome activation by modulating reactive oxygen species production.J immunol 2010;185(12):7699-705. google scholar
- Hosmer Jr DW, Lemeshow S, Sturdivant RX. Applied logistic regression: John Wiley & Sons; 2013 google scholar
Year 2025,
, 10 - 16, 28.03.2025
Abstract
References
- Meyerhoff WL, Kim CS, Paparella MM. Pathology of chronic otitis media. Ann Otol Rhinol Laryngol Suppl 1978;87(6):749-60. google scholar
- Gopen Q. Fundamental Otology: Pediatric & Adult Practice: JP Medical Ltd; 2012. google scholar
- Dayasena R, Dayasiri M, Jayasuriya C, Perera D. Aetiological agents in chronic suppurative otitis media in Sri Lanka. Med J Aust 2011;4(2):101. google scholar
- Vanneste P, Page C. Otitis media with effusion in children: Pathophysiology, diagnosis, and treatment. A review. J Otol 2019;14(2):33-9. google scholar
- Lamkanfi M, Dixit VM. Mechanisms and functions of inflammasomes. Cell 2014;157(5):1013-22. google scholar
- Zaki MH, Lamkanfi M, Kanneganti T-D. The Nlrp3 inflammasome: contributions to intestinal homeostasis. Trends Immunol 2011;32(4):171-9. google scholar
- Kariya S, Okano M, Zhao P, Maeda Y, Kataoka Y, Higaki T, et al. NLRP3 inflammasome expression in lipopolysaccharide-induced otitis media. Acta Otolaryngol 2018;138(12):1061-5. google scholar
- Chien H, Dix RD. Evidence for multiple cell death pathways during development of experimental cytomegalovirus retinitis in mice with retrovirus-induced immunosuppression: apoptosis, necroptosis, and pyroptosis. J Virol 2012;86(20):10961-78. google scholar
- Taysi S, Memisogullari R, Koc M, Yazici AT, Aslankurt M, Gumustekin K, et al. Melatonin reduces oxidative stress in the rat lens due to radiation-induced oxidative injury. Int J Radiat Biol2008;84(10):803-8. google scholar
- Baysal E, Aksoy N, Kara F, Taysi S, Taşkın A, Bilinç H, et al. Oxidative stress in chronic otitis media. Eur Arch Otorhinolaryngol Suppl 2013;270:1203-8. google scholar
- Öktemer T, Kemaloğlu Y, Özbilen S, Tutar H. Kronik Otitis Media Cerrahisinde Greft Başarisini Etkileyen Faktörler. Osmangazi Tıp Dergisi 2016;38(3):46-54. google scholar
- Ford AH, Hankey GJ, Yeap BB, Golledge J, Flicker L, Almeida OP. Hearing loss and the risk of dementia in later life. Maturitas 2018;112:1-11. google scholar
- Karlı R, Karli A, Aksoy A, Açikgöz S. Orta kulak efüzyonlarında timpanogram ile otoskopik bulguların karşılaştırılması. Dicle Univ Tip Fakul Derg 2013;40(1):54-6. google scholar
- Xu Y-J, Zheng L, Hu Y-W, Wang Q. Pyroptosis and its relationship to atherosclerosis. Clin Chim Acta 2018;476:28-37. google scholar
- Martinon F, Burns K, Tschopp J. The inflammasome: a molecular platform triggering activation of inflammatory caspases and processing of proIL-β. Mol cell 2002;10(2):417-26. google scholar
- Man SM, Karki R, Kanneganti TD. Molecular mechanisms and functions of pyroptosis, inflammatory caspases and inflammasomes in infectious diseases. Immunol Rev 2017;277(1):61-75. google scholar
- Garça MF, Turan M, Avşar B, Kalkan F, Demir H, Kozan A, et al. The evaluation of oxidative stress in the serum and tissue specimens of patients with chronic otitis media. Clin Exp Otorhinolaryngol 2015;8(2):97-101. google scholar
- Halliwell B. Drug antioxidant effects: A basis for drug selection? Drugs 1991;42:569-605. google scholar
- Kariya S, Okano M, Zhao P, Kataoka Y, Yoshinobu J, Maeda Y, et al. Activation of NLRP3 inflammasome in human middle ear cholesteatoma and chronic otitis media. Acta Otolaryngol 2016;136(2):136-40. google scholar
- Karlıdağ T, İlhan N, Kaygusuz İ, Keleş E, Yalçn Ş. Comparison of free radicals and antioxidant enzymes in chronic otitis media with and without tympanosclerosis. Laryngoscope 2004;114(1):85-9. google scholar
- García Callejo F, Estors Ferrero J, Morant Ventura A, Segarra Cortés P, Velert Vila M. Lipoperoxidación en otorreas del oído medio como marcador de infección. Aplicación clínica. [Lipoperoxidation in otorrhea of the middle ear as a marker of infection. Clinical application]. Acta Otorrinolaringol Esp 2000;51(6):478-81. google scholar
- Aslan M, Çiçek MT. MPV ve NLO Değerleri kolesteatomu, kronik otitis mediadan ayırt eder mi? Van Tip Dergisi 2021;28(4):615-22. google scholar
- Macias MP, Gerkin RD, Macias JD. Increased amphiregulin expression as a biomarker of cholesteatoma activity. Laryngoscope 2010;120(11):2258-63. google scholar
- Lin H, Li Z, Lin D, Zheng C, Zhang W. Role of NLRP3 inflammasome in eosinophilic and non-eosinophilic chronic rhinosinusitis with nasal polyps. Inflammation 2016;39:2045-52. google scholar
- Wei Y, Zhang J, Wu X, Sun W, Wei F, Liu W, et al. Activated pyrin domain containing 3 (NLRP3) inflammasome in neutrophilic chronic rhinosinusitis with nasal polyps (CRSwNP). J Allergy Clin Immunol 2020;145(3):1002-5. google scholar
- Månsson A, Bogefors J, Cervin A, Uddman R, Cardell L-O. NOD‐like receptors in the human upper airways: a potential role in nasal polyposis. Allergy 2011;66(5):621-8. google scholar
- Kartlaşmış K, Dikmen N. Piroptozise biyokimyasal yaklaşım ve kanserdeki rolü. Arşiv Kaynak Tarama Dergisi 2020;29(3):181-9. google scholar
- Zhou R, Tardivel A, Thorens B, Choi I, Tschopp J. Thioredoxin-interacting protein links oxidative stress to inflammasome activation. Nat Immunol 2010;11(2):136-40. google scholar
- Salminen A, Ojala J, Kaarniranta K, Kauppinen A. Mitochondrial dysfunction and oxidative stress activate inflammasomes: impact on the aging process and age-related diseases. Cell Mol Life Sci 2012;69:2999-3013. google scholar
- Heid ME, Keyel PA, Kamga C, Shiva S, Watkins SC, Salter RD. Mitochondrial reactive oxygen species ınduces NLRP3-Dependent lysosomal damage and ınflammasome activation. J Immunol 2013;191(10):5230-8. google scholar
- Wu X, Zhang H, Qi W, Zhang Y, Li J, Li Z, et al. Nicotine promotes atherosclerosis via ROS-NLRP3-mediated endothelial cell pyroptosis. Cell Death Dis 2018;9(2):171. google scholar
- Nakahira K, Haspel JA, Rathinam VAK, Lee S-J, Dolinay T, Lam HC, et al. Autophagy proteins regulate innate immune responses by inhibiting the release of mitochondrial DNA mediated by the NALP3 inflammasome. Nat Immunol 2011;12(3):222-30. google scholar
- Hu Y, Mao K, Zeng Y, Chen S, Tao Z, Yang C, et al. Tripartite-motif protein 30 negatively regulates nlrp3 ınflammasome activation by modulating reactive oxygen species production.J immunol 2010;185(12):7699-705. google scholar
- Hosmer Jr DW, Lemeshow S, Sturdivant RX. Applied logistic regression: John Wiley & Sons; 2013 google scholar
There are 34 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Otorhinolaryngology |
| Journal Section | Research Articles |
| Authors | |
| Publication Date | March 28, 2025 |
| Submission Date | October 30, 2024 |
| Acceptance Date | February 26, 2025 |
| Published in Issue | Year 2025 |