Research Article
BibTex RIS Cite

Year 2025, Volume: 12 Issue: 2, 531 - 542, 16.04.2025

Melek Demirel , Yeter Çilesiz , Ecem Kara , Gökhan Baktemur

https://doi.org/10.30910/turkjans.1641798

Abstract

References

  • Abbey, B.W., Nwachoko, N., Ikiroma, G.N. (2017). Nutritional value of cucumber cultivated in three selected states of Nigeria. Biochemistry and Analytical Biochemistry 6: 328
  • Abdel-Farid, I.B., Marghany, M.R., Rowezek, M.M., Sheded, M.G. (2020). Effect of Salinity Stress on Growth and Metabolomic Profiling of Cucumis sativus and Solanum lycopersicum. Plants 9(11):1626.
  • Abu-Romman, S., Suwwan, M., Al-Zu’bi, E. (2012). Physiological effects of salinity on cucumber microshoots grown on proliferation medium. Adv. Environ. Biol, 6(11), 2829-2834.
  • Acosta-Motos, J. R., Ortuño, M. F., Bernal-Vicente, A., Diaz-Vivancos, P., Sanchez-Blanco, M. J., & Hernandez, J. A. (2017). Plant responses to salt stress: adaptive mechanisms. Agronomy, 7(1), 18.
  • Afsar, S., Bibi, G., Ahmad, R., Bilal, M., Naqvi, T. A., Baig, A., ... & Hussain, J. (2020). Evaluation of salt tolerance in Eruca sativa accessions based on morpho-physiological traits. Peer J, 8, e9749.
  • Aktaş, H. (2002). Biberde Tuza Dayanıklılığın Fizyolojik Karakterizasyonu ve Kalıtımı Doktora Tezi. Çukurova Üniversitesi, Fen Bilimleri Enstitüsü, 105 s.
  • Alrahman, N.A., Shibli, R.A., Ereifej, K., Hindiyeh, M.Y. (2005). Influence of salinity on growth and physiology of in vitro grown cucumber (Cucumis sativus L.). Jordan Journal of Agricultural Sciences, 1(1), 19.
  • Aydın, İ., Atıcı, Ö. (2015). Effects of Salt Stress on Germination and Seedling Development in Some Crop Plants. Muş Alparslan University Journal of Sciences. 3 (2). 1-15
  • Baktemur, G. (2023). Effect of Nutrient Media Including Sodium Chloride (NaCl) at Different Concentration on Squash (Cucurbita pepo L.) Plant Growth Under In Vitro Conditions. OKU Journal of The Institute of Science and Technology, 6(1), 873-882.
  • Chinnusamy, V., Jagendorf, A. Zhu, J. (2005). Understanding and improving salt tolerance in plants. Crop Sci. 45, 437–448
  • Daşgan, H.Y., Aktas, H., Abak, K., Çakmak, İ. (2002). Determination of screening techniques to salinity tolerance in tomatoes and ınvestigation of genotype responses. Plant Science, 163:695–703.
  • FAO, (2022). Food and Agriculture Organization of the United Nations. FAOSTAT. http://faostat.fao.org/ Last access date:24/08/2024.
  • Gelaye, Y. (2023). Cucumber (Cucumis sativus) production in Ethiopia: Trends, prospects and challenges: A review. Cogent Food & Agriculture, 9(1), 2221103.
  • Gill, S., Ramzan, M., Naz, G., Ali, L., Danish, S., Ansari, M.J., Salmen, S. H. (2024). Effect of silicon nanoparticle-based biochar on wheat growth, antioxidants and nutrients concentration under salinity stress. Scientific Reports, 14(1), 6380.
  • Gupta, N., Kumar, S., Jain, S.K., Tomar, B.S., Singh, J., Sharma, V. (2021). Challanges and opportunities in cucumber seed production. Int. J. Curr Microbiol. App. Sci, 10(1), 2135-2144.
  • Güldüren, Ş. (2012). Kuzey Doğu Anadolu Bölgesi ve Çoruh Vadisi’nden Toplanan Bazı Fasulye (Phaseolus vulgaris L.) Genotiplerinin Tuza Toleransı. Yüksek Lisans Tezi, Atatürk Üniversitesi, Fen Bilimleri Enstitüsü, 94 s. Hernández, J. A. (2019). Salinity tolerance in plants: Trends and perspectives. International Journal of Molecular Sciences, 20(10), 2408.
  • Kanwal, R., Maqsood, M. F., Shahbaz, M., Naz, N., Zulfiqar, U., Ali, M. F., ... & Alsakkaf, W. A. (2024). Exogenous ascorbic acid as a potent regulator of antioxidants, osmo-protectants, and lipid peroxidation in pea under salt stress. BMC Plant Biology, 24(1), 247.
  • Kara, E., Taşkın, H., Baktemur, G. (2024). Determination the Effects of Different Concentrations of Salt (NaCl) Added to the Nutrient Medium under in vitro Conditions on the Development of Tomato (Solanum lycopersicumL.). ISPEC Journal of Agricultural Sciences, 8(2), 301-309.
  • Kaya, M.D., Okçu, G., Atak, M., Çıkılı, Y., Kolsarıcı, Ö. (2006). Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). European Journal of Agronomy, 24(4): 291-295.
  • Keleş, B. (2019). Morphological, Physiological And Biochemical Changes in Safflower (Carthamus tinctorius L.) Germinating under in vitro Culture Conditions and Salinity (NaCl) Stress. MS Thesis, The Graduate School Of Natural And Applied Science of Batman Unıversity, 61 s.
  • Khayatnezhad, M. Gholamin, R. (2011). Effects of Salt Stress Levels on Five Maize (Zea mays L.) Cultivars at Germination Stage. African Journal of Biotechnology, 10(60), 12909-12915
  • Kıran, S., Kuşvuran, Ş., Özkay, F., Özgün, Ö., Sönmez, K., Özbek, H., Ellialtıoğlu, Ş.Ş. (2015). Comparison of Development of Some Eggplant Rootstock in the Salinity Stress Conditions. Reserach Journal of Agricultural Sciences, 8(1): 20-30.
  • Kurum, R., Ulukapı, K., Aydınşakir, K., Onus, N.A. (2013). The influence of salinity on seedling growth of some pumpkin varieties used as rootstock. Not Bot Horti Agrobo, 41(1):219-225.
  • Kuşvuran, Ş. (2010). Relationships Between Physiological Mechanisms of Tolerances To Drought And Salınıty In Melons. PhD Thesis. Cukurova Unıversity Institute of Science and Science, 355 s.
  • Kuşvuran, Ş. (2011). Investigation of Screening Parameters and Genotypic Differences for Salt Tolerance In Okra (Abelmoschus esculentus L.). Derim, 28(2), 55-70.
  • Mahmood, M.S., Pırlak, L. (2023). Aronya (Aronia melanocarpa) fidanlarının in vitro ve in vivo şartlarda tuz stresine toleranslarının belirlenmesi. International Conference on Scientific and Innovative Studies 1: 86–91.
  • Masuda, T., Yonemori, S., Oyama, Y., Takeda, Y., Tanaka T., Andoh. T. (1999). Evaluation of the antioxidant activity of environmental plants: activity of the leaf extracts from seashore plants. J. Agric. Food Chem. 47: 1749–1754.
  • Murashige T., Skoog, F., (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant, 15:473–497
  • Nasrin, F., Bulbul, I.J., Aktar, F., Rashid, M.A. (2015). Anti-inflammatory and antioxidant activities of cucumis sativus leaves. Bangladesh Pharmaceutical Journal, 18(2), 169-173.
  • Onimisi, A.O., Ovansa, J.U. (2015) Comparative studies on nutritional values of four varieties of cucumber Proceedings International Conference on Latest Trends in Food, Biological Conference and Ecological Sciences (ICLTFBE’15) Dubai (UAE) 11-12
  • Öztekin, G.B., Tüzel, Y. (2011). Salinity response of some tomato rootstocks at seedling stage. African Journal of Agricultural Research, 6(20): 4726-4735.
  • Öztürk Gökçe, N.Z., Atik, H., Vural, M., Gökçe, A.F. (2022). Determination of Germination Characteristic of Seeds of Some Onion (Allium cepa L.) Breeding Lines under in vitro Conditions with Different Salt Concentration. Alatarım, 21 (1): 1-9
  • Pandey, S., Kujur, S.N. (2022). Importance, Distribution, Botany and Genetics, the Cucumber Genome. Springer International Publishing.
  • Rahman, M., Soomro, U.A., Haq, M.Z. Gul, S. (2008). Effects Of Nacl Salinity on Wheat (Triticum aestivum L.) Cultivars. World Journal of Agricultural Sciences 4 (3): 398-403
  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radical biology and medicine, 26(9-10), 1231-1237. Sarwar, M., Anjum, S., Ali, Q. et al. (2021). Triacontanol modulates salt stress tolerance in cucumber by altering the physiological and biochemical status of plant cells. Sci Rep 11, 24504.
  • Shariff, A.H.M., Baharin, S., Wahab, R.A., Huyop, F., Mohamad, N. R., Zakaria, M., ... & Julmohammad, N. (2021). Antioxidant activity, total phenolic and chlorophyll content of Keningau grown Cucumis sativus L. at two growth stages. Jurnal Teknologi, 83(3), 37-44.
  • Singh, P., Gopal, J. (2019). Effect of Water and Salinity Stress on Germination and Seedling Characters in Onion. Indian Journal of Horticulture. 76. 368-372
  • Tabassum, M., Noreen, Z., Aslam, M., Shah, A. N., Usman, S., Waqas, A., ... & Nazim, M. (2024). Chitosan modulated antioxidant activity, inorganic ions homeostasis and endogenous melatonin to improve yield of Pisum sativum L. accessions under salt stress. Scientia Horticulturae, 323, 112509.
  • TÜİK, (2023). Turkish Statistical Institute. www.tuik.gov.tr/ Last access date: 24/08/2024.
  • Xu, J., Xu, Y., Wang, Y., Lv, Z., Liu, X., Sun, W., ... & Zhang, C. (2024). Exogenous salicylic acid improves photosynthetic and antioxidant capacities and alleviates adverse effects of cherry rootstocks under salt stress. Journal of Plant Growth Regulation, 43(5), 1428-1446.
  • Zia-ur-Rehman, M., Anayatullah, S., Irfan, E., Hussain, S. M., Rizwan, M., Sohail, M. I., ... & Alharby, H. F. (2023). Nanoparticles assisted regulation of oxidative stress and antioxidant enzyme system in plants under salt stress: A review. Chemosphere, 314, 137649.

Effect of Salinity Stress on Plant Growth Parameters and Antioxidant Activity In Some Cucumber Cultivars (Cucumis sativus L.) Grown Under In Vitro Conditions

Year 2025, Volume: 12 Issue: 2, 531 - 542, 16.04.2025

Melek Demirel , Yeter Çilesiz , Ecem Kara , Gökhan Baktemur

https://doi.org/10.30910/turkjans.1641798

Abstract

In this study, effects of salinity (NaCl) at different concentrations (0, 50, 100, 150, 200, 250, 300 mM) on the growth of cucumber plants were determined in in vitro conditions. The study was carried out in the plant tissue culture laboratory of Agricultural Sciences and Technology Faculty of Sivas Science and Technology University (Sivas, Türkiye) . Experiments were conducted with 3 different cucumber varieties. Mureshige and Skoog (MS) was used as the basic nutrient medium. During the study, some parameters were evaluated such as germination rate (%), salt tolerance index (%), stem fresh and dry weight (g), root fresh and dry weight (g), actual water content (%), stem and root lengths and visual scale values. Also, antioxidant capacity were determined by DPPH and ABTS. When the effect of different NaCl doses on the varieties was evaluated, the highest antioxidant capacity was obtained from HD medium (173.21 μmol TE g-1 dw) in the DPPH analysis and from HC medium (251.06 μmol TE g-1 dw) in the ABTS analysis. In the study, the highest germination rate was obtained in the variety 1 (94.36%) and in the HA medium (96.05%). In the actual water content, the variety average was the highest in the variety 2 (91.16%) and the medium average was the highest in the HA (91.71%). The highest stem length was determined in the variety 1 (26.40 mm) and in the HA medium (24.48 mm). Considering the results of the study, although there were differences among the varieties, the increase in NaCl concentration negatively affected germination and plant growth in cucumber.

Keywords

In vitro NaCl Cucumber Antioxidant

References

  • Abbey, B.W., Nwachoko, N., Ikiroma, G.N. (2017). Nutritional value of cucumber cultivated in three selected states of Nigeria. Biochemistry and Analytical Biochemistry 6: 328
  • Abdel-Farid, I.B., Marghany, M.R., Rowezek, M.M., Sheded, M.G. (2020). Effect of Salinity Stress on Growth and Metabolomic Profiling of Cucumis sativus and Solanum lycopersicum. Plants 9(11):1626.
  • Abu-Romman, S., Suwwan, M., Al-Zu’bi, E. (2012). Physiological effects of salinity on cucumber microshoots grown on proliferation medium. Adv. Environ. Biol, 6(11), 2829-2834.
  • Acosta-Motos, J. R., Ortuño, M. F., Bernal-Vicente, A., Diaz-Vivancos, P., Sanchez-Blanco, M. J., & Hernandez, J. A. (2017). Plant responses to salt stress: adaptive mechanisms. Agronomy, 7(1), 18.
  • Afsar, S., Bibi, G., Ahmad, R., Bilal, M., Naqvi, T. A., Baig, A., ... & Hussain, J. (2020). Evaluation of salt tolerance in Eruca sativa accessions based on morpho-physiological traits. Peer J, 8, e9749.
  • Aktaş, H. (2002). Biberde Tuza Dayanıklılığın Fizyolojik Karakterizasyonu ve Kalıtımı Doktora Tezi. Çukurova Üniversitesi, Fen Bilimleri Enstitüsü, 105 s.
  • Alrahman, N.A., Shibli, R.A., Ereifej, K., Hindiyeh, M.Y. (2005). Influence of salinity on growth and physiology of in vitro grown cucumber (Cucumis sativus L.). Jordan Journal of Agricultural Sciences, 1(1), 19.
  • Aydın, İ., Atıcı, Ö. (2015). Effects of Salt Stress on Germination and Seedling Development in Some Crop Plants. Muş Alparslan University Journal of Sciences. 3 (2). 1-15
  • Baktemur, G. (2023). Effect of Nutrient Media Including Sodium Chloride (NaCl) at Different Concentration on Squash (Cucurbita pepo L.) Plant Growth Under In Vitro Conditions. OKU Journal of The Institute of Science and Technology, 6(1), 873-882.
  • Chinnusamy, V., Jagendorf, A. Zhu, J. (2005). Understanding and improving salt tolerance in plants. Crop Sci. 45, 437–448
  • Daşgan, H.Y., Aktas, H., Abak, K., Çakmak, İ. (2002). Determination of screening techniques to salinity tolerance in tomatoes and ınvestigation of genotype responses. Plant Science, 163:695–703.
  • FAO, (2022). Food and Agriculture Organization of the United Nations. FAOSTAT. http://faostat.fao.org/ Last access date:24/08/2024.
  • Gelaye, Y. (2023). Cucumber (Cucumis sativus) production in Ethiopia: Trends, prospects and challenges: A review. Cogent Food & Agriculture, 9(1), 2221103.
  • Gill, S., Ramzan, M., Naz, G., Ali, L., Danish, S., Ansari, M.J., Salmen, S. H. (2024). Effect of silicon nanoparticle-based biochar on wheat growth, antioxidants and nutrients concentration under salinity stress. Scientific Reports, 14(1), 6380.
  • Gupta, N., Kumar, S., Jain, S.K., Tomar, B.S., Singh, J., Sharma, V. (2021). Challanges and opportunities in cucumber seed production. Int. J. Curr Microbiol. App. Sci, 10(1), 2135-2144.
  • Güldüren, Ş. (2012). Kuzey Doğu Anadolu Bölgesi ve Çoruh Vadisi’nden Toplanan Bazı Fasulye (Phaseolus vulgaris L.) Genotiplerinin Tuza Toleransı. Yüksek Lisans Tezi, Atatürk Üniversitesi, Fen Bilimleri Enstitüsü, 94 s. Hernández, J. A. (2019). Salinity tolerance in plants: Trends and perspectives. International Journal of Molecular Sciences, 20(10), 2408.
  • Kanwal, R., Maqsood, M. F., Shahbaz, M., Naz, N., Zulfiqar, U., Ali, M. F., ... & Alsakkaf, W. A. (2024). Exogenous ascorbic acid as a potent regulator of antioxidants, osmo-protectants, and lipid peroxidation in pea under salt stress. BMC Plant Biology, 24(1), 247.
  • Kara, E., Taşkın, H., Baktemur, G. (2024). Determination the Effects of Different Concentrations of Salt (NaCl) Added to the Nutrient Medium under in vitro Conditions on the Development of Tomato (Solanum lycopersicumL.). ISPEC Journal of Agricultural Sciences, 8(2), 301-309.
  • Kaya, M.D., Okçu, G., Atak, M., Çıkılı, Y., Kolsarıcı, Ö. (2006). Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). European Journal of Agronomy, 24(4): 291-295.
  • Keleş, B. (2019). Morphological, Physiological And Biochemical Changes in Safflower (Carthamus tinctorius L.) Germinating under in vitro Culture Conditions and Salinity (NaCl) Stress. MS Thesis, The Graduate School Of Natural And Applied Science of Batman Unıversity, 61 s.
  • Khayatnezhad, M. Gholamin, R. (2011). Effects of Salt Stress Levels on Five Maize (Zea mays L.) Cultivars at Germination Stage. African Journal of Biotechnology, 10(60), 12909-12915
  • Kıran, S., Kuşvuran, Ş., Özkay, F., Özgün, Ö., Sönmez, K., Özbek, H., Ellialtıoğlu, Ş.Ş. (2015). Comparison of Development of Some Eggplant Rootstock in the Salinity Stress Conditions. Reserach Journal of Agricultural Sciences, 8(1): 20-30.
  • Kurum, R., Ulukapı, K., Aydınşakir, K., Onus, N.A. (2013). The influence of salinity on seedling growth of some pumpkin varieties used as rootstock. Not Bot Horti Agrobo, 41(1):219-225.
  • Kuşvuran, Ş. (2010). Relationships Between Physiological Mechanisms of Tolerances To Drought And Salınıty In Melons. PhD Thesis. Cukurova Unıversity Institute of Science and Science, 355 s.
  • Kuşvuran, Ş. (2011). Investigation of Screening Parameters and Genotypic Differences for Salt Tolerance In Okra (Abelmoschus esculentus L.). Derim, 28(2), 55-70.
  • Mahmood, M.S., Pırlak, L. (2023). Aronya (Aronia melanocarpa) fidanlarının in vitro ve in vivo şartlarda tuz stresine toleranslarının belirlenmesi. International Conference on Scientific and Innovative Studies 1: 86–91.
  • Masuda, T., Yonemori, S., Oyama, Y., Takeda, Y., Tanaka T., Andoh. T. (1999). Evaluation of the antioxidant activity of environmental plants: activity of the leaf extracts from seashore plants. J. Agric. Food Chem. 47: 1749–1754.
  • Murashige T., Skoog, F., (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant, 15:473–497
  • Nasrin, F., Bulbul, I.J., Aktar, F., Rashid, M.A. (2015). Anti-inflammatory and antioxidant activities of cucumis sativus leaves. Bangladesh Pharmaceutical Journal, 18(2), 169-173.
  • Onimisi, A.O., Ovansa, J.U. (2015) Comparative studies on nutritional values of four varieties of cucumber Proceedings International Conference on Latest Trends in Food, Biological Conference and Ecological Sciences (ICLTFBE’15) Dubai (UAE) 11-12
  • Öztekin, G.B., Tüzel, Y. (2011). Salinity response of some tomato rootstocks at seedling stage. African Journal of Agricultural Research, 6(20): 4726-4735.
  • Öztürk Gökçe, N.Z., Atik, H., Vural, M., Gökçe, A.F. (2022). Determination of Germination Characteristic of Seeds of Some Onion (Allium cepa L.) Breeding Lines under in vitro Conditions with Different Salt Concentration. Alatarım, 21 (1): 1-9
  • Pandey, S., Kujur, S.N. (2022). Importance, Distribution, Botany and Genetics, the Cucumber Genome. Springer International Publishing.
  • Rahman, M., Soomro, U.A., Haq, M.Z. Gul, S. (2008). Effects Of Nacl Salinity on Wheat (Triticum aestivum L.) Cultivars. World Journal of Agricultural Sciences 4 (3): 398-403
  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radical biology and medicine, 26(9-10), 1231-1237. Sarwar, M., Anjum, S., Ali, Q. et al. (2021). Triacontanol modulates salt stress tolerance in cucumber by altering the physiological and biochemical status of plant cells. Sci Rep 11, 24504.
  • Shariff, A.H.M., Baharin, S., Wahab, R.A., Huyop, F., Mohamad, N. R., Zakaria, M., ... & Julmohammad, N. (2021). Antioxidant activity, total phenolic and chlorophyll content of Keningau grown Cucumis sativus L. at two growth stages. Jurnal Teknologi, 83(3), 37-44.
  • Singh, P., Gopal, J. (2019). Effect of Water and Salinity Stress on Germination and Seedling Characters in Onion. Indian Journal of Horticulture. 76. 368-372
  • Tabassum, M., Noreen, Z., Aslam, M., Shah, A. N., Usman, S., Waqas, A., ... & Nazim, M. (2024). Chitosan modulated antioxidant activity, inorganic ions homeostasis and endogenous melatonin to improve yield of Pisum sativum L. accessions under salt stress. Scientia Horticulturae, 323, 112509.
  • TÜİK, (2023). Turkish Statistical Institute. www.tuik.gov.tr/ Last access date: 24/08/2024.
  • Xu, J., Xu, Y., Wang, Y., Lv, Z., Liu, X., Sun, W., ... & Zhang, C. (2024). Exogenous salicylic acid improves photosynthetic and antioxidant capacities and alleviates adverse effects of cherry rootstocks under salt stress. Journal of Plant Growth Regulation, 43(5), 1428-1446.
  • Zia-ur-Rehman, M., Anayatullah, S., Irfan, E., Hussain, S. M., Rizwan, M., Sohail, M. I., ... & Alharby, H. F. (2023). Nanoparticles assisted regulation of oxidative stress and antioxidant enzyme system in plants under salt stress: A review. Chemosphere, 314, 137649.
There are 41 citations in total.

Details

Primary Language English
Subjects Agricultural Marine Biotechnology
Journal Section Research Article
Authors

Melek Demirel 0000-0002-8477-1122

Yeter Çilesiz 0000-0002-4313-352X

Ecem Kara 0000-0002-0118-2673

Gökhan Baktemur 0000-0002-0362-5108

Publication Date April 16, 2025
Submission Date February 20, 2025
Acceptance Date April 14, 2025
Published in Issue Year 2025 Volume: 12 Issue: 2

Cite

APA Demirel, M., Çilesiz, Y., Kara, E., Baktemur, G. (2025). Effect of Salinity Stress on Plant Growth Parameters and Antioxidant Activity In Some Cucumber Cultivars (Cucumis sativus L.) Grown Under In Vitro Conditions. Turkish Journal of Agricultural and Natural Sciences, 12(2), 531-542. https://doi.org/10.30910/turkjans.1641798
 Download Cover Image