Research Article
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Year 2025, Volume: 12 Issue: 3, 552 - 564, 23.07.2025

Aycan Bayav , Dicle Dönmez , Songul Comlekcioglu , Ayzin Küden , Yıldız Aka Kaçar

https://doi.org/10.30910/turkjans.1656992

Abstract

References

  • Ahmadizadeh, M., Nori, A., Shahbazi, H., & Habibpour, M. (2011). Effects of drought stress on some agronomic and morphological traits of durum wheat (Triticum durum Desf.) landraces under greenhouse condition. African Journal of Biotechnology, 10(64), 14097-14107.
  • Aka Kaçar, Y. (2004). Using of molecular markers in Prunus species. Alatarım, 3(2), 15-22.
  • Aka Kaçar, Y., Lezzoni, A.F., & Çetiner, S. (2005). Sweet cherry cultivar identification by using SSR markers. Journal of Biological Sciences, 5(5), 616-619. https://doi.org/10.3923/jbs.2005.616.619
  • Aka Kacar, Y., Simsek, O., Donmez, D., Boncuk, M., Yeşiloğlu, T., & Ollitrault, P. (2014). Genetic relationships of some Citrus genotypes based on the candidate iron chlorosis genes. Turkish Journal of Agriculture and Forestry, 38, 340–347. https://doi.org/10.3906/tar-1301-15
  • Akçura, M. (2011). The relationships of some traits in Turkish winter bread wheat landraces. Turkish Journal of Agriculture and Forestry, 35(2), 115-125. https://doi.org/10.3906/tar-0908-301
  • Akcura, M., Turan, V., Kokten, K., & Kaplan, M. (2019). Fatty acid and some micro element compositions of cluster bean (Cyamopsis tetragonoloba) genotype seeds growing under Mediterranean climate. Industrial Crops and Products, 128, 140-146.
  • Anonymous (2025). Türkiye Hayvansal üretim istatistikleri. https://data.tuik.gov.tr/Bulten/Index?p=Hayvansal-Uretim-Istatistikleri-2024-53935 (Accessed date: 10.02.2025)
  • Anonymous, (2019). Food and Agriculture Organization of the United Nations (FAO), http://www.fao.org/site, (Accessed date: 13.11.2018)
  • Çömlekçioğlu, S. (2014). Identification of plant characteristics of nectarine and peach hybrids and usability of some ssr markers in early selection. (Ph. D. Thesis) Cukurova University. The Institute of Natural Sciences, Adana, Türkiye.
  • Domblides, A., & Domblides, E. (2023). Rapid genetic assessment of carrot varieties based on AFLP analysis. Horticulturae, 9(3), 298. https://doi.org/10.3390/horticulturae9030298
  • Dosba, F. (2002). Progress and prospects in stone fruit breeding. In XXVI International Horticultural Congress: Genetics and Breeding of Tree Fruits and Nuts, 622, 35-43.
  • Edwards. K., Johnstone, C., & Thompson, C. (1991). A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Research, 19(6), 1349. https://doi.org/10.1093/nar/19.6.1349
  • Eroğlu, Z.Ö., & Mısırlı, A. (2012). Peach breeding and development. Bahce, 41(2), 37-46.
  • Filiz, E., & Koç, İ. (2011). Molecular markers in plant biotechnology. Journal of Agricultural Faculty of Gaziosmanpasa University, 2, 207-214.
  • Genişel, H. (2013). Characterization of new putative species crocus (Colchicum L.) in Turkey’s flora using ISSR markers. (MSc Thesis) Istanbul University. Institute of Science, İstanbul, Türkiye.
  • Goulão, L., Monte-Corvo, L., & Oliveira, C.M. (2001). Phenetic characterization of plum cultivars by high multiplex ratio markers: Amplified fragment length polymorphisms and inter-simple sequence repeats. Journal of the American Society for Horticultural Science, 126(1), 72-77.
  • Güleç, T.E., Yıldırım, A., & Sönmezoğlu, Ö.A. (2010). Marker assisted selection in plants. Turkish Journal of Scientific Reviews, 2, 67-79.
  • Hammer, D.A.T., & Harper, P.D.R. (2001). Past: paleontological statistics software package for education and data analysis. Palaeontologia Electronica, 4(1), 4-9.
  • Hoisington, M.A., Seferis, J.C., & Thompson, D. (1992). Scale-up for hot melt prepreg manufacturing. In 37th International SAMPE Symposium and Exhibition. 264-277.
  • Kaynaş, K., Alkın, G., & Çiftci, H.N. (2022). Effects of 1-methylcyclopropane and modified atmosphere packaging on quality characteristics in storage of ANET 30 peach varieties. COMU Journal of Agriculture Faculty, 10(1), 99-107.
  • Kumar, R., Dimri, D.C., Karki, K., Rai, K.M., Singh, N.K., Shivran, J.S., & Bharti, S. (2023). SSR marker based profiling and population structure analysis in peach (Prunus persica) germplasm. Indian Journal of Agricultural Sciences, 93(10), 1080-1085. https://doi.org/10.56093/ijas.v93i10.132658
  • LaRue, J. (1989). Introduction. In: LaRue, JH, Johnson RS (eds) Peaches, plums, and nectarines: growing and handling for fresh market. Publication No 3331. University of California, Division of Agriculture and Natural Resources, Oakland, California, pp. 1-2.
  • Li, W., Ma, Y., Kou, Y., Zeng, Z., Qiu, D., & Ma, C. (2023). Analysis of genetic diversity and relationships between late-mature peach (Prunus persica L.) varieties assessed with ISSR and SRAP markers. Fruit Research, 3(36), 1-10. https://doi.org/10.48130/FruRes-2023-0036
  • Mahmood, A.M., Faraj, J.M., Sharef, A.A., Majeed, H.O., Hamasalih, F.M., & Tahir, N.A. (2023). Evaluation of wild almond genotypes grown as a rain-fed crop in sulaimani governorate using RAPD and ISSR markers. Kufa Journal for Agricultural Sciences, 15(1), 19-33.
  • Mantel, N. (1967). The detection of disease clustering and a generalized regression approach. Cancer Research, 27, 209-220.
  • Mirik, M. (2005). Biberde bakteriyel leke etmeni Xanthomonas axonopodis pv. vesicatoria’nın tanılanması ve bitki büyüme düzenleyici rizobakteriler ile biyolojik mücadele olanakları. (Ph. D. Thesis) Cukurova University. The Institute of Natural Sciences, Adana, Turkey
  • Mohsenin, N.N. (1970). Physical Properties of Plant and Animal Materials. Gordon and Breach Science Publishers, New York
  • Okie, W.R., Bacon, T., & Bassi, D. (2008). Fresh market cultivar development. The peach: Botany, production and uses. CABI, Cambridge, 139-174.
  • Powell, W., Machray, G.C., & Provan, J. (1996). Polymorphism revealed by simple sequence repeats. Trends in Plant Science, 1(7), 215-222.
  • Saglam, C., & Tan, F. (2017). An Environmentally Friendly Method of Fertilizer Application in Agriculture; Anhydrous Ammonia Application. International Conference on Environment and Natural Science. (ICENS). 9-10 September, P.7-9. Moscow, Russia.
  • Sang, Q., Zhou, Z., Mu, J., & Wang, L. (2021). Genetic factors as potential molecular markers of human oocyte and embryo quality. Journal of Assistant Reproduction and Genetics, 38(5), 993-1002. https://doi.org/10.1007/s10815-021-02196-z
  • Schulman, A.H. (2007). Molecular markers to assess genetic diversity. Euphytica, 158(3), 313-321.
  • Şimşek, Ö., Karaat, F.E., Serçe, S., & Aka Kaçar, Y. (2008). Comparison of the efficiency of DNA isolation methods in some fruit species. Derim, 25(1), 59-69.
  • Smith, J.S.C., Chin, E.C.L., Shu, H., Smith, O.S., Wall, S.J., Senior, M.L., & Ziegle, J. (1997). An evaluation of the utility of SSR loci as molecular markers in maize (Zea mays L.): comparisons with data from RFLPs and pedigree. Theoretical and Applied Genetics, 95(1–2), 163–173.
  • Wünsch, A. (2009). Cross-transferable polymorphic SSR loci in Prunus species. Scientia Horticulturae, 120, 348-352. https://doi.org/10.1016/j.scienta.2008.11.012
  • Yang, K., Chen, D.H., Ding, X., Li, J., Zhang, Y.Q., Zhang, T., et al., Wang, X.M. (2023). Different roles of primary and secondary sources in reducing PM2. 5: Insights from molecular markers in Pearl River Delta, South China. Atmospheric Environment, 294, 119487. https://doi.org/10.1016/j.atmosenv.2022.119487
  • Yorgancılar, M., Yakışır, E., & Erkoyuncu, M.T. (2015). Using of molecular markers in plant breeding. Journal Bahri Dagdas Herbal Research, 4(2), 1-12.
  • Yu, W., Peng, F., Wang, W., Liang, J., Xiao, Y., & Yuan, X. (2021). SnRK1 phosphorylation of SDH positively regulates sorbitol metabolism and promotes sugar accumulation in peach fruit. Tree Physiology, 41(6), 1077-1086. https://doi.org/10.1093/treephys/tpaa163
  • Yüce, M. (2018). Determination of performance of some peach and nectarine varieties in ecological conditions of Kahramanmaraş province. (MSc Thesis) Kahramanmaraş Sütçü İmam University, Institute of Science, Kahramanmaraş, Türkiye.

Molecular characterization of some peach and nectarine hybrids by ISSR and SSR markers

Year 2025, Volume: 12 Issue: 3, 552 - 564, 23.07.2025

Aycan Bayav , Dicle Dönmez , Songul Comlekcioglu , Ayzin Küden , Yıldız Aka Kaçar

https://doi.org/10.30910/turkjans.1656992

Abstract

The Prunus genus includes valuable fruit trees such as cherries, almonds, apricots, plums, peaches, and nectarines. Peaches and nectarines are among the most widely cultivated fruits globally and can adapt to temperate and subtropical climate conditions. In this study, the genetic relationships of 96 peach and nectarine hybrids were analyzed using Simple Sequence Repeat (SSR) and Simple Intersequence Repeat (ISSR) markers. These genotypes consist of hybrid VxÜ (Venüs x Üstün) and RxÜ (Stark Red Gold x Üstün) obtained through the hybridization of peach and nectarine varieties. 5 SSR and 14 ISSR primers were used for both VxÜ and RxÜ genotypes. In the SSR analysis of hybrid VxÜ and RxÜ genotypes, 12 bands were obtained, all polymorphic, resulting in a polymorphism rate of 100%. The band size ranged from 150 to 240 bp. The PIC values ranged from 0.32 to 0.91, with an average of 0.62. In the ISSR analysis of hybrid RxÜ and VxÜ genotypes, 163 bands were obtained, of which 150 were polymorphic and polymorphism rate of 91%. The band size range varied between 300 and 2000 bp. Genetic similarity values among genotypes were calculated, and a UPGMA cluster analysis was performed to create a dendrogram. The data and dendrograms obtained from genetic analysis were compared. The dendrograms generated from the SSR and ISSR analyses were divided into two main clusters. In the dendrogram based on SSR analysis, the VÜ-103 hybrid formed a distinct group, separate from all other genotypes. Conversely, in the dendrogram derived from ISSR analysis, the VÜ hybrids clustered within the first main branch, while the RU hybrids were grouped within the second main branch. The results of the study showed that both SSR and ISSR markers are potential tools for determining genetic diversities and genetic relationships of Prunus hybrids and can be used in breeding programs.

Keywords

Peach Nectarine Molecular Characterization SSR ISSR

References

  • Ahmadizadeh, M., Nori, A., Shahbazi, H., & Habibpour, M. (2011). Effects of drought stress on some agronomic and morphological traits of durum wheat (Triticum durum Desf.) landraces under greenhouse condition. African Journal of Biotechnology, 10(64), 14097-14107.
  • Aka Kaçar, Y. (2004). Using of molecular markers in Prunus species. Alatarım, 3(2), 15-22.
  • Aka Kaçar, Y., Lezzoni, A.F., & Çetiner, S. (2005). Sweet cherry cultivar identification by using SSR markers. Journal of Biological Sciences, 5(5), 616-619. https://doi.org/10.3923/jbs.2005.616.619
  • Aka Kacar, Y., Simsek, O., Donmez, D., Boncuk, M., Yeşiloğlu, T., & Ollitrault, P. (2014). Genetic relationships of some Citrus genotypes based on the candidate iron chlorosis genes. Turkish Journal of Agriculture and Forestry, 38, 340–347. https://doi.org/10.3906/tar-1301-15
  • Akçura, M. (2011). The relationships of some traits in Turkish winter bread wheat landraces. Turkish Journal of Agriculture and Forestry, 35(2), 115-125. https://doi.org/10.3906/tar-0908-301
  • Akcura, M., Turan, V., Kokten, K., & Kaplan, M. (2019). Fatty acid and some micro element compositions of cluster bean (Cyamopsis tetragonoloba) genotype seeds growing under Mediterranean climate. Industrial Crops and Products, 128, 140-146.
  • Anonymous (2025). Türkiye Hayvansal üretim istatistikleri. https://data.tuik.gov.tr/Bulten/Index?p=Hayvansal-Uretim-Istatistikleri-2024-53935 (Accessed date: 10.02.2025)
  • Anonymous, (2019). Food and Agriculture Organization of the United Nations (FAO), http://www.fao.org/site, (Accessed date: 13.11.2018)
  • Çömlekçioğlu, S. (2014). Identification of plant characteristics of nectarine and peach hybrids and usability of some ssr markers in early selection. (Ph. D. Thesis) Cukurova University. The Institute of Natural Sciences, Adana, Türkiye.
  • Domblides, A., & Domblides, E. (2023). Rapid genetic assessment of carrot varieties based on AFLP analysis. Horticulturae, 9(3), 298. https://doi.org/10.3390/horticulturae9030298
  • Dosba, F. (2002). Progress and prospects in stone fruit breeding. In XXVI International Horticultural Congress: Genetics and Breeding of Tree Fruits and Nuts, 622, 35-43.
  • Edwards. K., Johnstone, C., & Thompson, C. (1991). A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Research, 19(6), 1349. https://doi.org/10.1093/nar/19.6.1349
  • Eroğlu, Z.Ö., & Mısırlı, A. (2012). Peach breeding and development. Bahce, 41(2), 37-46.
  • Filiz, E., & Koç, İ. (2011). Molecular markers in plant biotechnology. Journal of Agricultural Faculty of Gaziosmanpasa University, 2, 207-214.
  • Genişel, H. (2013). Characterization of new putative species crocus (Colchicum L.) in Turkey’s flora using ISSR markers. (MSc Thesis) Istanbul University. Institute of Science, İstanbul, Türkiye.
  • Goulão, L., Monte-Corvo, L., & Oliveira, C.M. (2001). Phenetic characterization of plum cultivars by high multiplex ratio markers: Amplified fragment length polymorphisms and inter-simple sequence repeats. Journal of the American Society for Horticultural Science, 126(1), 72-77.
  • Güleç, T.E., Yıldırım, A., & Sönmezoğlu, Ö.A. (2010). Marker assisted selection in plants. Turkish Journal of Scientific Reviews, 2, 67-79.
  • Hammer, D.A.T., & Harper, P.D.R. (2001). Past: paleontological statistics software package for education and data analysis. Palaeontologia Electronica, 4(1), 4-9.
  • Hoisington, M.A., Seferis, J.C., & Thompson, D. (1992). Scale-up for hot melt prepreg manufacturing. In 37th International SAMPE Symposium and Exhibition. 264-277.
  • Kaynaş, K., Alkın, G., & Çiftci, H.N. (2022). Effects of 1-methylcyclopropane and modified atmosphere packaging on quality characteristics in storage of ANET 30 peach varieties. COMU Journal of Agriculture Faculty, 10(1), 99-107.
  • Kumar, R., Dimri, D.C., Karki, K., Rai, K.M., Singh, N.K., Shivran, J.S., & Bharti, S. (2023). SSR marker based profiling and population structure analysis in peach (Prunus persica) germplasm. Indian Journal of Agricultural Sciences, 93(10), 1080-1085. https://doi.org/10.56093/ijas.v93i10.132658
  • LaRue, J. (1989). Introduction. In: LaRue, JH, Johnson RS (eds) Peaches, plums, and nectarines: growing and handling for fresh market. Publication No 3331. University of California, Division of Agriculture and Natural Resources, Oakland, California, pp. 1-2.
  • Li, W., Ma, Y., Kou, Y., Zeng, Z., Qiu, D., & Ma, C. (2023). Analysis of genetic diversity and relationships between late-mature peach (Prunus persica L.) varieties assessed with ISSR and SRAP markers. Fruit Research, 3(36), 1-10. https://doi.org/10.48130/FruRes-2023-0036
  • Mahmood, A.M., Faraj, J.M., Sharef, A.A., Majeed, H.O., Hamasalih, F.M., & Tahir, N.A. (2023). Evaluation of wild almond genotypes grown as a rain-fed crop in sulaimani governorate using RAPD and ISSR markers. Kufa Journal for Agricultural Sciences, 15(1), 19-33.
  • Mantel, N. (1967). The detection of disease clustering and a generalized regression approach. Cancer Research, 27, 209-220.
  • Mirik, M. (2005). Biberde bakteriyel leke etmeni Xanthomonas axonopodis pv. vesicatoria’nın tanılanması ve bitki büyüme düzenleyici rizobakteriler ile biyolojik mücadele olanakları. (Ph. D. Thesis) Cukurova University. The Institute of Natural Sciences, Adana, Turkey
  • Mohsenin, N.N. (1970). Physical Properties of Plant and Animal Materials. Gordon and Breach Science Publishers, New York
  • Okie, W.R., Bacon, T., & Bassi, D. (2008). Fresh market cultivar development. The peach: Botany, production and uses. CABI, Cambridge, 139-174.
  • Powell, W., Machray, G.C., & Provan, J. (1996). Polymorphism revealed by simple sequence repeats. Trends in Plant Science, 1(7), 215-222.
  • Saglam, C., & Tan, F. (2017). An Environmentally Friendly Method of Fertilizer Application in Agriculture; Anhydrous Ammonia Application. International Conference on Environment and Natural Science. (ICENS). 9-10 September, P.7-9. Moscow, Russia.
  • Sang, Q., Zhou, Z., Mu, J., & Wang, L. (2021). Genetic factors as potential molecular markers of human oocyte and embryo quality. Journal of Assistant Reproduction and Genetics, 38(5), 993-1002. https://doi.org/10.1007/s10815-021-02196-z
  • Schulman, A.H. (2007). Molecular markers to assess genetic diversity. Euphytica, 158(3), 313-321.
  • Şimşek, Ö., Karaat, F.E., Serçe, S., & Aka Kaçar, Y. (2008). Comparison of the efficiency of DNA isolation methods in some fruit species. Derim, 25(1), 59-69.
  • Smith, J.S.C., Chin, E.C.L., Shu, H., Smith, O.S., Wall, S.J., Senior, M.L., & Ziegle, J. (1997). An evaluation of the utility of SSR loci as molecular markers in maize (Zea mays L.): comparisons with data from RFLPs and pedigree. Theoretical and Applied Genetics, 95(1–2), 163–173.
  • Wünsch, A. (2009). Cross-transferable polymorphic SSR loci in Prunus species. Scientia Horticulturae, 120, 348-352. https://doi.org/10.1016/j.scienta.2008.11.012
  • Yang, K., Chen, D.H., Ding, X., Li, J., Zhang, Y.Q., Zhang, T., et al., Wang, X.M. (2023). Different roles of primary and secondary sources in reducing PM2. 5: Insights from molecular markers in Pearl River Delta, South China. Atmospheric Environment, 294, 119487. https://doi.org/10.1016/j.atmosenv.2022.119487
  • Yorgancılar, M., Yakışır, E., & Erkoyuncu, M.T. (2015). Using of molecular markers in plant breeding. Journal Bahri Dagdas Herbal Research, 4(2), 1-12.
  • Yu, W., Peng, F., Wang, W., Liang, J., Xiao, Y., & Yuan, X. (2021). SnRK1 phosphorylation of SDH positively regulates sorbitol metabolism and promotes sugar accumulation in peach fruit. Tree Physiology, 41(6), 1077-1086. https://doi.org/10.1093/treephys/tpaa163
  • Yüce, M. (2018). Determination of performance of some peach and nectarine varieties in ecological conditions of Kahramanmaraş province. (MSc Thesis) Kahramanmaraş Sütçü İmam University, Institute of Science, Kahramanmaraş, Türkiye.
There are 39 citations in total.

Details

Primary Language English
Subjects Plant Biotechnology in Agriculture
Journal Section Research Article
Authors

Aycan Bayav 0009-0003-4320-7175

Dicle Dönmez 0000-0002-7446-9405

Songul Comlekcioglu 0000-0003-1275-4574

Ayzin Küden 0000-0002-0811-6695

Yıldız Aka Kaçar 0000-0001-5314-7952

Publication Date July 23, 2025
Submission Date March 13, 2025
Acceptance Date July 3, 2025
Published in Issue Year 2025 Volume: 12 Issue: 3

Cite

APA Bayav, A., Dönmez, D., Comlekcioglu, S., Küden, A., et al. (2025). Molecular characterization of some peach and nectarine hybrids by ISSR and SSR markers. Turkish Journal of Agricultural and Natural Sciences, 12(3), 552-564. https://doi.org/10.30910/turkjans.1656992
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