Research Article
BibTex RIS Cite

Determination of hematological and biochemical parameters in healthy Arabian and thoroughbred racehorses

Year 2025, Volume: 10 Issue: 1, 26 - 34, 30.04.2025
https://doi.org/10.31797/vetbio.1565704

Abstract

In this study, it was aimed to determine the differences in terms of race, age, and gender by examining the hemogram and biochemical parameters of healthy thoroughbred racehorses brought to the equine hospital for routine examination. The study material comprised 86 healthy horses (51 Thoroughbred and 35 Arabian Horses) of both genders (31 female and 55 male). The horses were divided into three groups according to their ages (3 years and younger, 4 years, and 5 years and older). Blood samples were analyzed on the hemogram and biochemistry devices after sampling. There was no statistically significant difference in the parameters examined between the breeds (p>0.05). Hematologically, it was determined that white blood cell (WBC), lymphocyte (LYM), mean corpuscular hemoglobin (MCH), and red cell distribution width (RDW) values were at the highest level in those aged 3 and younger, and neutrophil (NEU), mean corpuscular volume (MCV), and MCH values were at the highest level in 5 years and older. Biochemically, it was determined that aspartate transferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), albumin (ALB), and phosphors (P) values were at the highest level in those aged 3 and younger, and total protein (TP) values were at the highest level in those aged 5 and older. In terms of gender, WBC, red blood cell (RBC), Mean corpuscular hemoglobin concentration (MCHC), RDW, AST, ALP, gamma-glutamyl transferase (GGT), LDH, Creatine kinase (CK), creatinine (CREA), ALB, and P values were higher in males compared to females, while MCV, MCH, Mean platelet volume (MPV), and TP values were higher in females compared to males. As a result, although the Thoroughbred and Arabian Racehorses examined in the study were healthy, there might be significant differences in hemogram and biochemistry values in terms of age and gender.

Thanks

We would like to thank the Turkish Jockey Club Adana Hippodrome for providing the horse blood data.

References

  • Adamu, L., Noraniza, M. A., Rasedee, A., & Bashir, A. (2013). Effect of age and performance on physical, hematological, and biochemical parameters in endurance horses. Journal of Equine Veterinary Science, 33(6), 415-420. https://doi.org/10.1016/j.jevs.2012.07.015.
  • Akyüz, E., Ölmez, M., Kuru, M., Merhan, O., Makav, M., Öğün, M., Bozukluhan K, Naseri A, Uzlu E., & Gökçe, G. (2020). Gürcü keçilerinde mera öncesi, merada ve mera sonrası dönemde bazı biyokimyasal parametrelerin değerlendirilmesi. Dicle Üniversitesi Veteriner Fakültesi Dergisi, 13(1), 33-38.
  • AL-Hadithy, H. A. H., (2011). Assessment of some liver enzyme activities i n healthy Iraqi Racing Horses. The Iraqi Journal of Veterinary Medicine, 35(1), 76-80. https://doi.org/10.30539/iraqijvm.v35i1.606.
  • Alilovic, I., Rukavina, D., Ajanovic, A., Crnkic, C., Ohran, H., & Zahirovic, A. (2022). Breed, age, and sex-related variations in hematological and some biochemical parameters in the Tornjak dog. Turkish Journal of Veterinary & Animal Sciences, 46(2), 192-200. https://doi.org/10.55730/1300-0128.4166.
  • Allaam, M., Elseady, Y., Nayel, M., Elsify, A., Salama, A., Hassan, H., Hassan M., & Kamar, A. (2014). Physiological and hemato-chemical evaluation of thoroughbred race horse after exercise. International Journal for Agro Veterinary and Medical Sciences, 8(3), 81-93.
  • Altınsaat, Ç. (2008). The effects of age and gender on blood parameters in Arabian horses. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 14(2), 173-178. https://doi.org/10.9775/kvfd.2008.40-a.
  • Arslan, M., Özcan, M., Tosun, C., Çötelioğlu, Ü., & Matur, E. (2002). The effects of physical exercise on some plasma enzymes and Ca and P levels in race horses. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, 28(1), 91-97.
  • Ayhan, H., & Gürgöze, S. (2024). Safkan ingiliz ve arap atlarında hematolojik parametreler üzerine ırk, yaş ve cinsiyetin etkileri. Dicle Üniversitesi Veteriner Fakültesi Dergisi, 17(1), 31-35. https://doi.org/10.47027/duvetfd.1399988.
  • Babaeski, S. (2023). Taylarda Sütten Kesilme Sürecinde Immunmodülatör Uygulamasının Etkilerinin Değerlendirilmesi. (Tez No. 789471). [Doktora Tezi, Bursa Uludağ Üniversitesi Sağlık Bilimleri Enstitüsü Veteriner Fakültesi İç Hastalıkları Anabilim Dalı]. Yükseköğretim Kurulu Ulusal Tez Merkezi.
  • Benashour, F., Shmela, M., Salheen, S., Alnagar, F., & Al-Zlitni, R. (2024). Effect of age on the biochemical and hematological blood profile in the Arabian horses raised in Libya. AlQalam Journal of Medical and Applied Sciences, 7(3), 698-708. https://doi.org/10.54361/ajmas.247335.
  • Bilal, T. & Meral, Y. (2002). İngiliz ve Arap Atlarında hematolojik değerler. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, 28(1), 199-207. https://doi.org/10.16988/iuvfd.04890
  • Bonhomme, M. M., Patarin, F., Kruse, C. J., François, A. C., Renaud, B., Couroucé, A., Leleu, C., Boemer, F., Toquet, M. P., Richard, E. A., Seignot, J., Wouters, C. P., & Votion, D. M. (2023). Untargeted metabolomics profiling reveals exercise intensity-dependent alterations in thoroughbred racehorses’ plasma after routine conditioning sessions. ACS Omega, 8(50), 48557-48571. https://doi.org/10.1021/acsomega.3c08583.s002
  • Braithwaite, G.D. (1975). Studies on the absorption and retention of calcium and phosphorus by young and mature Ca-deficient sheep. British Journal of Nutrition, 34(2), 311-324. https://doi.org/10.1017/s0007114575000359.
  • Burlikowska, K., Boguslawska-Tryk, M., Szymeczko, R., & Piotrowska, A. (2015). Haematological and biochemical blood parameters in horses used for sport and recreation. Journal Central European Agriculture, 16(4), 370-382. https://doi.org/10.5513/jcea.v16i4.3806.
  • Cebulj-Kadunc, N., Bozic, M., Kosec, M., & Cestnik, V. (2002). The influence of age and gender on haematological parameters in Lipizzan horses. Journal of Veterinary Medicine Series. A, Physiology, Pathology, Clinical Medicine, 49(4), 217-221. https://doi.org/10.1046/j.1439-0442.2002.00439.x.
  • Comba, B., Mert, H., Comba, A., Mis, L., & Mert, N. (2017). The some hematological and biochemical parameters in Karakul and Norduz sheep. Van Veterinary Journal, 28(3), 137-140.
  • Czech, A., Kiesz, M., Kiesz, A., Próchniak, T., Różański, P., & Klimiuk, K. (2019). Influence of type of use, age and gender on haematological and biochemical blood parameters of małopolski horses. Annals of Animal Science, 19(1), 85-96. https://doi.org/10.2478/aoas-2018-0031.
  • Çötelioğlu, Ü., Aslan, M., Matur, E., Bakırel, U., Özcan, M., & Tosun, C. (2001). The effects of physical exercise on some physiological parameters, plasma CK and LDH levels in horses that are breeded as race horses. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, 27(2), 609-615.
  • da Conceição, F. F. C. B., de Mattos, B. H. V., Sá, P. A., Marçola, T. G., da Silva, G. A., Teixeira, H. C. A., & Campebell, R. C. (2022). Hematological and biochemical values in Breton breed horses in Brasília-DF. Brazilian Journal of Veterinary Medicine, 44, e001122. https://doi.org/10.29374/2527-2179.bjvm001122.
  • de Oliveira, K., Fachiolli, D. F., Watanabe, M. J., Tsuzukibashi, D., Bittar, C. M. M., Costa, C., Poiatti, M. L., & Meirelles, P. R. L. (2015). Dimethylglycine supplementation in horses performing incremental treadmill exercise. Comparative Exercise Physiology, 11(3), 167-172. https://doi.org/10.3920/cep150016.
  • Demirel, M., Eren, M., & Şentürk, M. (2022). Atlarda egzersizin nitrik oksit, interlöykin-10 düzeyleri ve kaspaz-6 aktivitesi ile bazı biyokimyasal parametrelere etkisi. Sağlık Bilimleri Dergisi, 31(2), 204-209. https://doi.org/10.34108/eujhs.1039027.
  • Demirtaş, B. (2018). Breed and gender-related differences in some hematological parameters of 3-year-old horses in flat racing in Turkey. Eurasian Journal of Veterinary Science, 34(1), 7-12. https://doi.org/10.15312/eurasianjvetsci.2018.173.
  • Essen-Gustavsson, B., & Lindholm, A. (1985). Muscle fibre characteristics of active and inactive standardbred horses. Equine Veterinary Journal, 17(6), 434-438. https://doi.org/10.1111/j.2042-3306.1985.tb02549.x.
  • Fails, A.D. (2020). Functional anatomy of the equine musculoskeletal system. In Adams and Stashak's Lameness in Horses, G.M. Baxter (Ed.). https://doi.org/10.1002/9781119276715.ch1.
  • Fazio, F., Assenza, A., Tosto, F., Casella, S., Piccione, G., & Caola, G. (2011). Training and haematochemical profile in Thoroughbreds and Standardbreds: A longitudinal study. Livestock Science, 141(2-3), 221-226. https://doi.org/10.1016/j.livsci.2011.06.005.
  • Fenger, C. K., Tobin, T., Casey, P. J., Roualdes, E. A., Langemeier, J. L., & Haines, D. M. (2014). Bovine colostrum supplementation optimises earnings, performance and recovery in racing Thoroughbreds. Comparative Exercise Physiology, 10(4), 233-238. https://doi.org/10.3920/cep140023.
  • Gürgöze, S. Y., & İçen, H. (2010). The influence of age on clinical biochemical parameters in pure-bred Arabian mares. Journal of Equine Veterinary Science, 30(10), 569-574. https://doi.org/10.1016/j.jevs.2010.09.006.
  • Güzelbektaş, H., Ok, M., Şen, İ., & Coşkun, A. (2006). Atlarda uzun süreli fiziksel egzersizin hematolojik ve bazı biyokimyasal parametreler üzerinde etkisi. Veteriner Bilimleri Dergisi, 22(1-2), 27-30.
  • Halo, Jr., M., Massányi, M., Strečanský, M., Kováčik, A., Formicki, G., Greń, A., Halo, M., & Massányi, P. (2020). The effect of age on biochemical parameters on horses. Scientific Papers Animal Science and Biotechnologies, 53(2), 117-117.
  • Harking, J. D., Morris, G. S., Tulley, R. T., Nelson, A. G., & Kamerling, S. G. (2022). Effect of added dietary fat on racing performance in thoroughbred horses. Journal of Equine Veterinary Science, 12(2), 123-129. https://doi.org/10.1016/s0737-0806(06)81295-5.
  • Harris, P. A., Marlin, D. J., & Gray, J. (1998). Plasma aspartate aminotransferase and creatine kinase activities in thoroughbred racehorses in relation to age, sex, exercise and training. The Veterinary Journal, 155(3), 295-304. https://doi.org/10.1016/s1090-0233(05)80026-7.
  • Hinchcliff, K. W., & Geor, R.J. (2008). The horse as an athlete: a physiological overview. In K. W. Hinchcliff, R. J. Geor, & A. J. Kaneps (Eds.), Equine Exercise Physiology (pp. 2–11). Edinburgh: W. B. Saunders. https://doi.org/10.1016/B978-070202857-1.50003-2.
  • Hodgson, D. R., & Rose, R. J. (1994). The athletic horse: in principles and practice of equine sports medicine. Saunders Publishing. https://doi.org/10.1016/S0737-0806(06)81980-5.
  • Hodgson, D. R., & McGowan, C. (2014). An overview of performance and sports medicine. The Athletic Horse, Principles and Practice of Equine Sports Medicine, 2, 1-8. https://doi.org/10.1016/b978-0-7216-0075-8.00010-1.
  • Isović, J., Čamo, D., Ćutuk, R., & Zahirović. A. (2023). The influence of various physical activity on hematological and biochemical parameters in draft and sport horses. International Journal of Veterinary Science, 12(5), 708-714. https://doi.org/10.47278/journal.ijvs/2023.014.
  • Jawor, P., Stefaniak, T., Borkowski, J., Przewoźny, M., Wierzbicki, H., & Swagemakers, J. H. (2007). Utility of selected blood parameters in monitoring the accuracy of show jump-horse training. Medycyna Weterynaryjna, 63(2), 213-218.
  • Kaneko, J.J., Harvey, J.W., & Bruss, M. L. (2008). Clinical biochemistry of domestic animals (6th ed). Academic press. https://doi.org/10.1016/B978-0-12-370491-7.X0001-3.
  • Kedzierski, W., Bergero, D., & Assenza, A. (2009). Trends of hematological and biochemical values in the blood of young racehorses during standardized field exercise tests. Acta Veterinaria, 59(5-6), 457-466. https://doi.org/10.2298/AVB0906457K.
  • Kelani, O. L., & Durotoye, L. A. (2002). Haematological responses of the African giant rat (Cricetomys gambianus) to castration and androgen replacement. Veterinarski Arhiv, 72(1), 39-49.
  • Kisadere, I., Bayraktar, M., & Salykov, R. (2019). Some hematological and biochemical reference values of the thoroughbred Appaloosa horse breeds reared in Kyrgyzstan. Comparative Clinical Pathology, 28(6), 1651-1660. https://doi.org/10.1007/s00580-019-02991-2.
  • Klobučar, K., Vrbanac, Z., Gotić, J., Bojanić, K., Bureš, T., & Bottegaro, N. B. (2019). Changes in biochemical parameters in horses during 40 km and 80 km endurance races. Acta Veterinaria, 69(1), 73-87. https://doi.org/10.2478/acve-2019-0005.
  • Kocaman, E., & Fidancı, U. R. (2016). Arap atlarında egzersizin lipid profili üzerine etkisi. Harran Üniversitesi Veteriner Fakültesi Dergisi, 5(2), 120-123.
  • Lassen, E. D., & Swardson, C. J. (1995). Hematology and hemostasis in the horse: normal functions and common abnormalities. Veterinary Clinics of North America: Equine Practice, 11(3), 351-389. https://doi.org/10.1016/s0749-0739(17)30306-1.
  • McFarlane, D., Sellon, D. C., & Gibbs, S. A. (2001). Age-related quantitative alterations in lymphocyte subsets and immunoglobulin isotypes in healthy horses. American Journal of Veterinary Research, 62(9), 1413-1417. https://doi.org/10.2460/ajvr.2001.62.1413.
  • Merck Veterinary Manuel. (2022). Serum Biochemical Analysis Reference Ranges. https://www.merckvetmanual.com/special-subjects/reference-guides/serum-biochemical-analysis-reference-ranges.
  • Mesarič, M., Nemec, M., & Kadunc, N. Č. (2023). Characterisation of the haematological profile in the Posavje horse breed. Slovenian Veterinary Research/Slovenski Veterinarski Zbornik, 60(1), 15-23. https://doi.org/10.26873/svr-1508-2023.
  • Miglio, A., Morelli, C., Maresca, C., Felici, A., Moscati, L., & Antognoni, M. T. (2019). Biochemical reference intervals for the Italian Heavy Draft horse. Comparative Clinical Pathology, 28, 841-851. https://doi.org/10.1007/s00580-019-02930-1.
  • Mikniene, Z., Maslauskas, K., Kerziene, S., Kučinskiene, & J., Kučinskas, A. (2014). The effect of age and gender on blood haematological and serum biochemical parameters in Žemaitukai horses. Veterinarija ir Zootechnika, 65 (87), 37-43.
  • Milojevic, M., Mitrovic, S., Ayaşan, T., Zdralic, T., & Djermanovic, V. (2020). Effects of age and sex of the foal on the gestation length of Thoroughbred mares. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 23(5), 1386-1392. https://doi.org/10.18016/ksutarimdoga.vi.692961.
  • Mukai, K., Ohmura, H., Takahashi, Y., Ebisuda, Y., Yoneda, K., & Miyata, H. (2023). Physiological and skeletal muscle responses to high-intensity interval exercise in Thoroughbred horses. Frontiers in Veterinary Science, 10, 1241266. https://doi.org/10.3389/fvets.2023.1241266.
  • Munoz, A., Riber, C., Trigo, P., & Castejón, F. (2012). Age-and gender-related variations in hematology, clinical biochemistry, and hormones in Spanish fillies and colts. Research in Veterinary Science, 93(2), 943-949. https://doi.org/10.1016/j.rvsc.2011.11.009.
  • Muñoz, A., Castejón-Riber, C., Riber, C., Esgueva, M., Trigo, P., & Castejón, F. (2017). Current knowledge of pathologic mechanisms and derived practical applications to prevent metabolic disturbances and exhaustion in the endurance horse. Journal of Equine Veterinary Science, 51, 24-33. https://doi.org/10.1016/j.jevs.2016.12.002.
  • Oktay, E., & Eren, M. (2014). Arap ve yerli melez atlarda bazı kan parametreleri üzerine ırk yaş ve cinsiyetin etkisi. Sağlık Bilimleri Dergisi, 23(2), 74-81.
  • Oruç, E., Kısadere, İ., Kadıralieva, N., & Sur, E. (2017). Kırgizistan Bişkek yöresi Konkur (engel) atlarında yarış öncesi ve sonrası bazı hematolojik, biyokimyasal analizler ile ANAE profili ve nazal eksfolyasyonun karşılaştırılması. Manas Journal of Agriculture Veterinary and Life Sciences, 7(1), 12-20.
  • Paden, L., Gomerčić, T., Đuras, M., Arbanasić, H., & Galov, A. (2014). Hematological and serum biochemical reference values for the Posavina and Croatian cold blood horse breeds. Acta Veterinaria, 64(2), 200-212. https://doi.org/10.2478/acve-2014-0019.
  • Paksoy, Y., Ünal, N. (2010). Atlarda Yarış Performansını Etkileyen Faktörler. Lalahan Hay. Araşt. Enst. Derg. 50 (2), 91-101.
  • Pourmohammed, R., Mohri, M., Aeifi, H., & Sardarı, K. (2019). Effect of exercise on some minerals, metabolites and enzyme activities in the serum of trained Arabian horses. Turkish Journal of Veterinary & Animal Sciences, 43(6), 791-799. https://doi.org/10.3906/vet-1905-82.
  • Satué, K., Hernández, A., Lorente, C., & O’Connor, J. E. (2010). Immunophenotypical characterization in Andalusian horse: variations with age and gender. Veterinary Immunology and Immunopathology, 133(2-4), 219-227. https://doi.org/10.1016/j.vetimm.2009.08.013.
  • Satué, K., Hernández, A., & Muñoz, A. (2012). Physiological factors in the interpretation of equine hematological profile. Hematology–Science and practice. Intech Publishing. https://doi.org/10.5772/38961.
  • Satue, K., Hernández, Á., Lorente, C., Fazio, E., & Pietro, M. (2020). Age-and sex-related modifications of hematology in Spanish purebred horse. Journal of Equine Veterinary Science, 93:103219. https://doi.org/10.1016/j.jevs.2020.103219.
  • Shawaf, T., Hussen, J., Al-Zoubi, M., Hamaash, H., & Al-Busadah, K. (2018). Impact of season, age and gender on some clinical, haematological and serum parameters in Shetland ponies in east province, Saudi Arabia. International Journal of Veterinary Science and Medicine, 6(1), 61-64. https://doi.org/10.1016/j.ijvsm.2018.03.007.
  • Smith, R., 3rd, Chaffin, M. K., Cohen, N. D., & Martens, R. J. (2002). Age-related changes in lymphocyte subsets of quarter horse foals. American Journal of Veterinary Research, 63(4), 531-537. https://doi.org/10.2460/ajvr.2002.63.531.
  • Souza, A. F., Schade, J., Kunz J. R,, Ramos, A. F., Albuquerque, M. S. M., Fonteque, G. V., Costa, D., Saito, M. E., & Fonteque, J. H. (2016). Serum biochemical profile from clinically healthy Campeiro horses. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 68(4), 839-844. https://doi.org/10.1590/1678-4162-8782.
  • Tepeoğlu, Y. (2018.) Orta mesafe koşan yarış atlarında performansın değerlendirilmesi ve yarış performansıyla ilişkisinin araştırılması. (Tez No. 523139). [Yüksek Lisans Tezi, Balıkesir Üniversitesi Sağlık Bilimleri Enstitüsü Beden Eğitimi ve Spor Anabilim Dalı]. Yükseköğretim Kurulu Ulusal Tez Merkezi.
  • Tomenendalova, J., Vodicka, R., Uhrikova, I., & Doubek, J. (2014). Determination of haematological and biochemical parameters of Przewalski horses (Equus Przewalski) kept by the Prague Zoo. Veterinární Medicína, 59(1), 11-21. https://doi.org/10.17221/7242-vetmed.
  • Turgut, K. (2000). Veteriner Klinik Laboratuar Teşhis, Genişletilmiş 2. Baskı. (pp. 346-382). Bahçıvanlar Basım Sanayi A.Ş., Konya.
  • Udeh, N. E., Orji, E., Madubuike, A. J., Ojiako, C., Okite, N., & Felix, G. C. (2021). Effect of season, age and sex on haematology and serum biochemistry of sport horses. Journal of Sustainable Veterinary & Allied Sciences, 1(2), 140-144. https://doi.org/10.54328/covm/josvas.2021.032.
  • Uluışık, D., Keskin, E., & Ozaydın, T. (2013). Age and gender related changes in hematological parameters of thoroughbred foals. Biotechnic & Histochemistry, 88(6), 345-349. https://doi.org/10.3109/10520295.2013.788213.
  • Waller, A. P., Heigenhauser, G. J. F., Geor, R. J., Spriet, L. L., & Lindinger, M.I. (2009). Fluid and electrolyte supplementation after prolonged moderate-intensity exercise enhances muscle glycogen resynthesis in Standardbred horses. Journal of Applied Physiology, 106(1), 91-100. https://doi.org/10.1152/japplphysiol.90783.2008.
  • Weiss, D. J., & Wardrop, K. J. (Eds.). (2011). Schalm's veterinary hematology. John Wiley & Sons. (6th ed.).
  • Zhang, Z., Gao, S., Dong, M., Luo, J., Xu, C., Wen, W., Huang, Y., Wu, Y., Zhou, J., & Yuan, Z. (2022). Relationship between red blood cell indices (MCV, MCH, and MCHC) and major adverse cardiovascular events in anemic and nonanemic patients with acute coronary syndrome. Disease Markers, 2022(1), 219334 https://doi.org/10.1155/2022/2193343.
Year 2025, Volume: 10 Issue: 1, 26 - 34, 30.04.2025
https://doi.org/10.31797/vetbio.1565704

Abstract

References

  • Adamu, L., Noraniza, M. A., Rasedee, A., & Bashir, A. (2013). Effect of age and performance on physical, hematological, and biochemical parameters in endurance horses. Journal of Equine Veterinary Science, 33(6), 415-420. https://doi.org/10.1016/j.jevs.2012.07.015.
  • Akyüz, E., Ölmez, M., Kuru, M., Merhan, O., Makav, M., Öğün, M., Bozukluhan K, Naseri A, Uzlu E., & Gökçe, G. (2020). Gürcü keçilerinde mera öncesi, merada ve mera sonrası dönemde bazı biyokimyasal parametrelerin değerlendirilmesi. Dicle Üniversitesi Veteriner Fakültesi Dergisi, 13(1), 33-38.
  • AL-Hadithy, H. A. H., (2011). Assessment of some liver enzyme activities i n healthy Iraqi Racing Horses. The Iraqi Journal of Veterinary Medicine, 35(1), 76-80. https://doi.org/10.30539/iraqijvm.v35i1.606.
  • Alilovic, I., Rukavina, D., Ajanovic, A., Crnkic, C., Ohran, H., & Zahirovic, A. (2022). Breed, age, and sex-related variations in hematological and some biochemical parameters in the Tornjak dog. Turkish Journal of Veterinary & Animal Sciences, 46(2), 192-200. https://doi.org/10.55730/1300-0128.4166.
  • Allaam, M., Elseady, Y., Nayel, M., Elsify, A., Salama, A., Hassan, H., Hassan M., & Kamar, A. (2014). Physiological and hemato-chemical evaluation of thoroughbred race horse after exercise. International Journal for Agro Veterinary and Medical Sciences, 8(3), 81-93.
  • Altınsaat, Ç. (2008). The effects of age and gender on blood parameters in Arabian horses. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 14(2), 173-178. https://doi.org/10.9775/kvfd.2008.40-a.
  • Arslan, M., Özcan, M., Tosun, C., Çötelioğlu, Ü., & Matur, E. (2002). The effects of physical exercise on some plasma enzymes and Ca and P levels in race horses. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, 28(1), 91-97.
  • Ayhan, H., & Gürgöze, S. (2024). Safkan ingiliz ve arap atlarında hematolojik parametreler üzerine ırk, yaş ve cinsiyetin etkileri. Dicle Üniversitesi Veteriner Fakültesi Dergisi, 17(1), 31-35. https://doi.org/10.47027/duvetfd.1399988.
  • Babaeski, S. (2023). Taylarda Sütten Kesilme Sürecinde Immunmodülatör Uygulamasının Etkilerinin Değerlendirilmesi. (Tez No. 789471). [Doktora Tezi, Bursa Uludağ Üniversitesi Sağlık Bilimleri Enstitüsü Veteriner Fakültesi İç Hastalıkları Anabilim Dalı]. Yükseköğretim Kurulu Ulusal Tez Merkezi.
  • Benashour, F., Shmela, M., Salheen, S., Alnagar, F., & Al-Zlitni, R. (2024). Effect of age on the biochemical and hematological blood profile in the Arabian horses raised in Libya. AlQalam Journal of Medical and Applied Sciences, 7(3), 698-708. https://doi.org/10.54361/ajmas.247335.
  • Bilal, T. & Meral, Y. (2002). İngiliz ve Arap Atlarında hematolojik değerler. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, 28(1), 199-207. https://doi.org/10.16988/iuvfd.04890
  • Bonhomme, M. M., Patarin, F., Kruse, C. J., François, A. C., Renaud, B., Couroucé, A., Leleu, C., Boemer, F., Toquet, M. P., Richard, E. A., Seignot, J., Wouters, C. P., & Votion, D. M. (2023). Untargeted metabolomics profiling reveals exercise intensity-dependent alterations in thoroughbred racehorses’ plasma after routine conditioning sessions. ACS Omega, 8(50), 48557-48571. https://doi.org/10.1021/acsomega.3c08583.s002
  • Braithwaite, G.D. (1975). Studies on the absorption and retention of calcium and phosphorus by young and mature Ca-deficient sheep. British Journal of Nutrition, 34(2), 311-324. https://doi.org/10.1017/s0007114575000359.
  • Burlikowska, K., Boguslawska-Tryk, M., Szymeczko, R., & Piotrowska, A. (2015). Haematological and biochemical blood parameters in horses used for sport and recreation. Journal Central European Agriculture, 16(4), 370-382. https://doi.org/10.5513/jcea.v16i4.3806.
  • Cebulj-Kadunc, N., Bozic, M., Kosec, M., & Cestnik, V. (2002). The influence of age and gender on haematological parameters in Lipizzan horses. Journal of Veterinary Medicine Series. A, Physiology, Pathology, Clinical Medicine, 49(4), 217-221. https://doi.org/10.1046/j.1439-0442.2002.00439.x.
  • Comba, B., Mert, H., Comba, A., Mis, L., & Mert, N. (2017). The some hematological and biochemical parameters in Karakul and Norduz sheep. Van Veterinary Journal, 28(3), 137-140.
  • Czech, A., Kiesz, M., Kiesz, A., Próchniak, T., Różański, P., & Klimiuk, K. (2019). Influence of type of use, age and gender on haematological and biochemical blood parameters of małopolski horses. Annals of Animal Science, 19(1), 85-96. https://doi.org/10.2478/aoas-2018-0031.
  • Çötelioğlu, Ü., Aslan, M., Matur, E., Bakırel, U., Özcan, M., & Tosun, C. (2001). The effects of physical exercise on some physiological parameters, plasma CK and LDH levels in horses that are breeded as race horses. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, 27(2), 609-615.
  • da Conceição, F. F. C. B., de Mattos, B. H. V., Sá, P. A., Marçola, T. G., da Silva, G. A., Teixeira, H. C. A., & Campebell, R. C. (2022). Hematological and biochemical values in Breton breed horses in Brasília-DF. Brazilian Journal of Veterinary Medicine, 44, e001122. https://doi.org/10.29374/2527-2179.bjvm001122.
  • de Oliveira, K., Fachiolli, D. F., Watanabe, M. J., Tsuzukibashi, D., Bittar, C. M. M., Costa, C., Poiatti, M. L., & Meirelles, P. R. L. (2015). Dimethylglycine supplementation in horses performing incremental treadmill exercise. Comparative Exercise Physiology, 11(3), 167-172. https://doi.org/10.3920/cep150016.
  • Demirel, M., Eren, M., & Şentürk, M. (2022). Atlarda egzersizin nitrik oksit, interlöykin-10 düzeyleri ve kaspaz-6 aktivitesi ile bazı biyokimyasal parametrelere etkisi. Sağlık Bilimleri Dergisi, 31(2), 204-209. https://doi.org/10.34108/eujhs.1039027.
  • Demirtaş, B. (2018). Breed and gender-related differences in some hematological parameters of 3-year-old horses in flat racing in Turkey. Eurasian Journal of Veterinary Science, 34(1), 7-12. https://doi.org/10.15312/eurasianjvetsci.2018.173.
  • Essen-Gustavsson, B., & Lindholm, A. (1985). Muscle fibre characteristics of active and inactive standardbred horses. Equine Veterinary Journal, 17(6), 434-438. https://doi.org/10.1111/j.2042-3306.1985.tb02549.x.
  • Fails, A.D. (2020). Functional anatomy of the equine musculoskeletal system. In Adams and Stashak's Lameness in Horses, G.M. Baxter (Ed.). https://doi.org/10.1002/9781119276715.ch1.
  • Fazio, F., Assenza, A., Tosto, F., Casella, S., Piccione, G., & Caola, G. (2011). Training and haematochemical profile in Thoroughbreds and Standardbreds: A longitudinal study. Livestock Science, 141(2-3), 221-226. https://doi.org/10.1016/j.livsci.2011.06.005.
  • Fenger, C. K., Tobin, T., Casey, P. J., Roualdes, E. A., Langemeier, J. L., & Haines, D. M. (2014). Bovine colostrum supplementation optimises earnings, performance and recovery in racing Thoroughbreds. Comparative Exercise Physiology, 10(4), 233-238. https://doi.org/10.3920/cep140023.
  • Gürgöze, S. Y., & İçen, H. (2010). The influence of age on clinical biochemical parameters in pure-bred Arabian mares. Journal of Equine Veterinary Science, 30(10), 569-574. https://doi.org/10.1016/j.jevs.2010.09.006.
  • Güzelbektaş, H., Ok, M., Şen, İ., & Coşkun, A. (2006). Atlarda uzun süreli fiziksel egzersizin hematolojik ve bazı biyokimyasal parametreler üzerinde etkisi. Veteriner Bilimleri Dergisi, 22(1-2), 27-30.
  • Halo, Jr., M., Massányi, M., Strečanský, M., Kováčik, A., Formicki, G., Greń, A., Halo, M., & Massányi, P. (2020). The effect of age on biochemical parameters on horses. Scientific Papers Animal Science and Biotechnologies, 53(2), 117-117.
  • Harking, J. D., Morris, G. S., Tulley, R. T., Nelson, A. G., & Kamerling, S. G. (2022). Effect of added dietary fat on racing performance in thoroughbred horses. Journal of Equine Veterinary Science, 12(2), 123-129. https://doi.org/10.1016/s0737-0806(06)81295-5.
  • Harris, P. A., Marlin, D. J., & Gray, J. (1998). Plasma aspartate aminotransferase and creatine kinase activities in thoroughbred racehorses in relation to age, sex, exercise and training. The Veterinary Journal, 155(3), 295-304. https://doi.org/10.1016/s1090-0233(05)80026-7.
  • Hinchcliff, K. W., & Geor, R.J. (2008). The horse as an athlete: a physiological overview. In K. W. Hinchcliff, R. J. Geor, & A. J. Kaneps (Eds.), Equine Exercise Physiology (pp. 2–11). Edinburgh: W. B. Saunders. https://doi.org/10.1016/B978-070202857-1.50003-2.
  • Hodgson, D. R., & Rose, R. J. (1994). The athletic horse: in principles and practice of equine sports medicine. Saunders Publishing. https://doi.org/10.1016/S0737-0806(06)81980-5.
  • Hodgson, D. R., & McGowan, C. (2014). An overview of performance and sports medicine. The Athletic Horse, Principles and Practice of Equine Sports Medicine, 2, 1-8. https://doi.org/10.1016/b978-0-7216-0075-8.00010-1.
  • Isović, J., Čamo, D., Ćutuk, R., & Zahirović. A. (2023). The influence of various physical activity on hematological and biochemical parameters in draft and sport horses. International Journal of Veterinary Science, 12(5), 708-714. https://doi.org/10.47278/journal.ijvs/2023.014.
  • Jawor, P., Stefaniak, T., Borkowski, J., Przewoźny, M., Wierzbicki, H., & Swagemakers, J. H. (2007). Utility of selected blood parameters in monitoring the accuracy of show jump-horse training. Medycyna Weterynaryjna, 63(2), 213-218.
  • Kaneko, J.J., Harvey, J.W., & Bruss, M. L. (2008). Clinical biochemistry of domestic animals (6th ed). Academic press. https://doi.org/10.1016/B978-0-12-370491-7.X0001-3.
  • Kedzierski, W., Bergero, D., & Assenza, A. (2009). Trends of hematological and biochemical values in the blood of young racehorses during standardized field exercise tests. Acta Veterinaria, 59(5-6), 457-466. https://doi.org/10.2298/AVB0906457K.
  • Kelani, O. L., & Durotoye, L. A. (2002). Haematological responses of the African giant rat (Cricetomys gambianus) to castration and androgen replacement. Veterinarski Arhiv, 72(1), 39-49.
  • Kisadere, I., Bayraktar, M., & Salykov, R. (2019). Some hematological and biochemical reference values of the thoroughbred Appaloosa horse breeds reared in Kyrgyzstan. Comparative Clinical Pathology, 28(6), 1651-1660. https://doi.org/10.1007/s00580-019-02991-2.
  • Klobučar, K., Vrbanac, Z., Gotić, J., Bojanić, K., Bureš, T., & Bottegaro, N. B. (2019). Changes in biochemical parameters in horses during 40 km and 80 km endurance races. Acta Veterinaria, 69(1), 73-87. https://doi.org/10.2478/acve-2019-0005.
  • Kocaman, E., & Fidancı, U. R. (2016). Arap atlarında egzersizin lipid profili üzerine etkisi. Harran Üniversitesi Veteriner Fakültesi Dergisi, 5(2), 120-123.
  • Lassen, E. D., & Swardson, C. J. (1995). Hematology and hemostasis in the horse: normal functions and common abnormalities. Veterinary Clinics of North America: Equine Practice, 11(3), 351-389. https://doi.org/10.1016/s0749-0739(17)30306-1.
  • McFarlane, D., Sellon, D. C., & Gibbs, S. A. (2001). Age-related quantitative alterations in lymphocyte subsets and immunoglobulin isotypes in healthy horses. American Journal of Veterinary Research, 62(9), 1413-1417. https://doi.org/10.2460/ajvr.2001.62.1413.
  • Merck Veterinary Manuel. (2022). Serum Biochemical Analysis Reference Ranges. https://www.merckvetmanual.com/special-subjects/reference-guides/serum-biochemical-analysis-reference-ranges.
  • Mesarič, M., Nemec, M., & Kadunc, N. Č. (2023). Characterisation of the haematological profile in the Posavje horse breed. Slovenian Veterinary Research/Slovenski Veterinarski Zbornik, 60(1), 15-23. https://doi.org/10.26873/svr-1508-2023.
  • Miglio, A., Morelli, C., Maresca, C., Felici, A., Moscati, L., & Antognoni, M. T. (2019). Biochemical reference intervals for the Italian Heavy Draft horse. Comparative Clinical Pathology, 28, 841-851. https://doi.org/10.1007/s00580-019-02930-1.
  • Mikniene, Z., Maslauskas, K., Kerziene, S., Kučinskiene, & J., Kučinskas, A. (2014). The effect of age and gender on blood haematological and serum biochemical parameters in Žemaitukai horses. Veterinarija ir Zootechnika, 65 (87), 37-43.
  • Milojevic, M., Mitrovic, S., Ayaşan, T., Zdralic, T., & Djermanovic, V. (2020). Effects of age and sex of the foal on the gestation length of Thoroughbred mares. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 23(5), 1386-1392. https://doi.org/10.18016/ksutarimdoga.vi.692961.
  • Mukai, K., Ohmura, H., Takahashi, Y., Ebisuda, Y., Yoneda, K., & Miyata, H. (2023). Physiological and skeletal muscle responses to high-intensity interval exercise in Thoroughbred horses. Frontiers in Veterinary Science, 10, 1241266. https://doi.org/10.3389/fvets.2023.1241266.
  • Munoz, A., Riber, C., Trigo, P., & Castejón, F. (2012). Age-and gender-related variations in hematology, clinical biochemistry, and hormones in Spanish fillies and colts. Research in Veterinary Science, 93(2), 943-949. https://doi.org/10.1016/j.rvsc.2011.11.009.
  • Muñoz, A., Castejón-Riber, C., Riber, C., Esgueva, M., Trigo, P., & Castejón, F. (2017). Current knowledge of pathologic mechanisms and derived practical applications to prevent metabolic disturbances and exhaustion in the endurance horse. Journal of Equine Veterinary Science, 51, 24-33. https://doi.org/10.1016/j.jevs.2016.12.002.
  • Oktay, E., & Eren, M. (2014). Arap ve yerli melez atlarda bazı kan parametreleri üzerine ırk yaş ve cinsiyetin etkisi. Sağlık Bilimleri Dergisi, 23(2), 74-81.
  • Oruç, E., Kısadere, İ., Kadıralieva, N., & Sur, E. (2017). Kırgizistan Bişkek yöresi Konkur (engel) atlarında yarış öncesi ve sonrası bazı hematolojik, biyokimyasal analizler ile ANAE profili ve nazal eksfolyasyonun karşılaştırılması. Manas Journal of Agriculture Veterinary and Life Sciences, 7(1), 12-20.
  • Paden, L., Gomerčić, T., Đuras, M., Arbanasić, H., & Galov, A. (2014). Hematological and serum biochemical reference values for the Posavina and Croatian cold blood horse breeds. Acta Veterinaria, 64(2), 200-212. https://doi.org/10.2478/acve-2014-0019.
  • Paksoy, Y., Ünal, N. (2010). Atlarda Yarış Performansını Etkileyen Faktörler. Lalahan Hay. Araşt. Enst. Derg. 50 (2), 91-101.
  • Pourmohammed, R., Mohri, M., Aeifi, H., & Sardarı, K. (2019). Effect of exercise on some minerals, metabolites and enzyme activities in the serum of trained Arabian horses. Turkish Journal of Veterinary & Animal Sciences, 43(6), 791-799. https://doi.org/10.3906/vet-1905-82.
  • Satué, K., Hernández, A., Lorente, C., & O’Connor, J. E. (2010). Immunophenotypical characterization in Andalusian horse: variations with age and gender. Veterinary Immunology and Immunopathology, 133(2-4), 219-227. https://doi.org/10.1016/j.vetimm.2009.08.013.
  • Satué, K., Hernández, A., & Muñoz, A. (2012). Physiological factors in the interpretation of equine hematological profile. Hematology–Science and practice. Intech Publishing. https://doi.org/10.5772/38961.
  • Satue, K., Hernández, Á., Lorente, C., Fazio, E., & Pietro, M. (2020). Age-and sex-related modifications of hematology in Spanish purebred horse. Journal of Equine Veterinary Science, 93:103219. https://doi.org/10.1016/j.jevs.2020.103219.
  • Shawaf, T., Hussen, J., Al-Zoubi, M., Hamaash, H., & Al-Busadah, K. (2018). Impact of season, age and gender on some clinical, haematological and serum parameters in Shetland ponies in east province, Saudi Arabia. International Journal of Veterinary Science and Medicine, 6(1), 61-64. https://doi.org/10.1016/j.ijvsm.2018.03.007.
  • Smith, R., 3rd, Chaffin, M. K., Cohen, N. D., & Martens, R. J. (2002). Age-related changes in lymphocyte subsets of quarter horse foals. American Journal of Veterinary Research, 63(4), 531-537. https://doi.org/10.2460/ajvr.2002.63.531.
  • Souza, A. F., Schade, J., Kunz J. R,, Ramos, A. F., Albuquerque, M. S. M., Fonteque, G. V., Costa, D., Saito, M. E., & Fonteque, J. H. (2016). Serum biochemical profile from clinically healthy Campeiro horses. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 68(4), 839-844. https://doi.org/10.1590/1678-4162-8782.
  • Tepeoğlu, Y. (2018.) Orta mesafe koşan yarış atlarında performansın değerlendirilmesi ve yarış performansıyla ilişkisinin araştırılması. (Tez No. 523139). [Yüksek Lisans Tezi, Balıkesir Üniversitesi Sağlık Bilimleri Enstitüsü Beden Eğitimi ve Spor Anabilim Dalı]. Yükseköğretim Kurulu Ulusal Tez Merkezi.
  • Tomenendalova, J., Vodicka, R., Uhrikova, I., & Doubek, J. (2014). Determination of haematological and biochemical parameters of Przewalski horses (Equus Przewalski) kept by the Prague Zoo. Veterinární Medicína, 59(1), 11-21. https://doi.org/10.17221/7242-vetmed.
  • Turgut, K. (2000). Veteriner Klinik Laboratuar Teşhis, Genişletilmiş 2. Baskı. (pp. 346-382). Bahçıvanlar Basım Sanayi A.Ş., Konya.
  • Udeh, N. E., Orji, E., Madubuike, A. J., Ojiako, C., Okite, N., & Felix, G. C. (2021). Effect of season, age and sex on haematology and serum biochemistry of sport horses. Journal of Sustainable Veterinary & Allied Sciences, 1(2), 140-144. https://doi.org/10.54328/covm/josvas.2021.032.
  • Uluışık, D., Keskin, E., & Ozaydın, T. (2013). Age and gender related changes in hematological parameters of thoroughbred foals. Biotechnic & Histochemistry, 88(6), 345-349. https://doi.org/10.3109/10520295.2013.788213.
  • Waller, A. P., Heigenhauser, G. J. F., Geor, R. J., Spriet, L. L., & Lindinger, M.I. (2009). Fluid and electrolyte supplementation after prolonged moderate-intensity exercise enhances muscle glycogen resynthesis in Standardbred horses. Journal of Applied Physiology, 106(1), 91-100. https://doi.org/10.1152/japplphysiol.90783.2008.
  • Weiss, D. J., & Wardrop, K. J. (Eds.). (2011). Schalm's veterinary hematology. John Wiley & Sons. (6th ed.).
  • Zhang, Z., Gao, S., Dong, M., Luo, J., Xu, C., Wen, W., Huang, Y., Wu, Y., Zhou, J., & Yuan, Z. (2022). Relationship between red blood cell indices (MCV, MCH, and MCHC) and major adverse cardiovascular events in anemic and nonanemic patients with acute coronary syndrome. Disease Markers, 2022(1), 219334 https://doi.org/10.1155/2022/2193343.
There are 71 citations in total.

Details

Primary Language English
Subjects Veterinary Biochemistry
Journal Section Research Articles
Authors

Halil Yavuz 0000-0001-9226-2937

Yavuzkan Paksoy 0000-0002-0935-7693

Muhammet Hanifi Selvi 0000-0002-9785-9174

Nuri Altuğ 0000-0001-5805-0340

Publication Date April 30, 2025
Submission Date October 11, 2024
Acceptance Date January 12, 2025
Published in Issue Year 2025 Volume: 10 Issue: 1

Cite

APA Yavuz, H., Paksoy, Y., Selvi, M. H., Altuğ, N. (2025). Determination of hematological and biochemical parameters in healthy Arabian and thoroughbred racehorses. Journal of Advances in VetBio Science and Techniques, 10(1), 26-34. https://doi.org/10.31797/vetbio.1565704

22563   CABI-Logo_NEW_accessible.jpg   logo_world_of_journals_no_margin.png  download download   download   download        download