Öz
Tıbbi ve süs bitkisi olarak değerli bir bitki olan Oxalis triangularis'in in vitro çoğaltım protokolü belirlenmiştir. Deneysel çalışmalarda Oxalis triangularis yaprak ve petiol eksplantlarına, α-Naftalin asetik asit (NAA) (0-0.5-1- 2 mg/L) ve 6-Benzilaminopurin (BAP) (0.5-1 mg/L) bitki büyüme düzenleyicileri birlikte veya tek başına uygulanmıştır. Eksplant başına en iyi sürgün oluşumu ve en iyi yaprak oluşumu, sırasıyla 2 mg/L NAA ve 0.5 mg/L BAP bitki büyüme düzenleyicilerini içeren Murashige ve Skoog (MS) besiyerinde kültüre alınan yaprak eksplantlarında 5.67 adet ± 2,43 ve 4.00 ± 1,86 olarak elde edilmiştir. Elde edilen sürgünler kök gelişimi için 0.5 mg/L NAA içeren MS besin ortamına aktarılmıştır. Mikroçoğaltım işlemleri sonucunda elde edilen bitkiler başarıyla aklimatize edilmiştir.
Anahtar Kelimeler
Bitki doku kültürü mikroçoğaltım oksin sitokinin Oxalis triangularis
Kaynakça
- Abdalla, N., K. Moustafa, M. Elsharkawy, and A. A. Al-Huqail. 2022. An academic and technical overview on plantmicropropagation challenges. Horticulturae 8(8): 677-688.
- Agnihotri, S., V. Rai, M. Tiwari, and D. Singh. 2025. Elicitation of the Himalayan toothache relieving tree (Zanthoxylum armatum DC.): enhancing secondary metabolites in vitro shoot cultures. Plant Cell, Tissue and Organ Culture 9(1): 7-12.
- Athari, M., A. Alizadeh, and S. Rahimi. 2024. Study on physiological and morphological traits of purple shamrock (Oxalis triangularis A. St.-Hil.) as affected by humic acid under salinity stress. Acta agriculturae Slovenica 120(1): 1-11.
- Babaoğlu, M., M. Yorgancılar, and M. A. Akbudak. 2001. Doku Kültürü: Temel laboratuar teknikleri. s. 1-35. In: Babaoğlu, M., Gürel, E., Özcan, S. (Eds.). Bitki biyoteknolojisi. Doku kültürü ve uygulamaları. Cilt I. Selçuk Üniversitesi Yayınları. Konya.
- Chen, S., L. Wang, J. Li, and Y. Zhang. 2024. Establishment of in vitro regeneration system for leaves and petioles of Oxalis triangularis 'Purpurea'. Technology in Horticulture 4(1): 11-19.
- Dupra, K. J. B., M. A. Aceret. 2024. Bioactivities of purple shamrock (Oxalis triangularis) crude extract and evaluation of shamrock topical antibacterial gel. International Journal of Integrated Science and Technology 1(2): 436-465.
- Gürel, A., S. Hayta, and E. Yılmaz. 2013. Bitki hücre, doku ve organ kültürü uygulamaları. Ege Üniversitesi Basımevi.
- Huh, S., J. Kim, S. Lee, and H. Park. 2010. Melanogenesis inhibitory effect of fatty acid alkyl esters isolated from Oxalis triangularis. Biological and Pharmaceutical Bulletin 33(7): 1242-1245.
- Joshi, N., R. Sharma, and A. Singh. 2024. Production of secondary metabolites from medicinal plants through tissue culture. In Secondary Metabolites and Biotherapeutics 7(1): 63-77.
- Kaviani, B. 2015. Some useful information about micropropagation. Journal of Ornamental Plants 5(1): 29-40.
- Klaocheed, S., S. Rittirat, S.Kalawong, and K. Thammasiri. 2024. Maximization of micropropagule production in an attractive ornamental plant, Oxalis triangularis A. St.-Hil. Trends in Sciences 21(8): 7917-7917.
- Kocaçalışkan, İ. 2021. Doku ve Hücre Kültürü Teknikleri (3. Basım). Nobel Akademik Yayıncılık.
- Luo, B., L. Chen, G. Chen, Y. Wang, Q. Xie, X. Chen, and Z. Hu. 2022. Transcription and metabolism pathways of anthocyanins in purple clover (Oxalis triangularis A. St.-Hil.). Metabolites 12(12): 1290-1307.
- Martin, K. 2002. Rapid propagation of Holostemma ada-kodien Schult., a rare medicinal plant, through axillary bud multiplication and indirect organogenesis. Plant Cell Reports, 21, 112-117.
- Murashige, T. 1974. Plant Propagation through Tissue Cultures. Annual Review of Plant Physiology 25: 135-166.
- Pamplona, R. S., C. D. Dela Cruz, and M. A. Tayangona. 2023. Selected Laboratory Equipment and Kitchen Utensils as an Alternative Sterilization Method for Sterilizing Plant Tissue Culture Media. bioRxiv 10(1): 1-22.
- Pan, H., L. Zhang, Y. Li, and Z. Wang. 2024. Establishment of callus induction and plantlet regeneration systems of Peucedanum praeruptorum Dunn based on the tissue culture method. Plant Methods 20(1): 174-190.
- Pasternak, T. P., and D. Steinmacher. 2024. Plant growth regulation in cell and tissue culture in vitro. Plants 13(2): 327-341.
- Pazmiño-Durán, E. A., P. Loyola, and F. Díaz. 2001. Anthocyanins from Oxalis triangularis as potential food colorants. Food Chemistry 75(2): 211-216.
- Prasuna, S., and B. Srinivas. 2015. In vitro regeneration studies of Oxalis corniculata from nodal explants. Int J Recent Sci Res 6: 6216-6220.
- Preil, W. 2003. Micropropagation of ornamental plants. In: Plant Tissue Culture: 100 Years Since Gottlieb Haberlandt pp. 115-133. Springer Vienna, Vienna.
- Ren, J., Q. Zhu, Y. Meng, F. Tang, Y. Xuan, D. Shi, S. Fu. 2017. Study on the Phenotypic Mutation Generated from 60Coγ-ray Irradiated Oxalias triangularis Purpurea Regeneration System. Agricultural Science & Technology 18(5): 756-762.
- Rittirat, S., S. Klaocheed, and K. Thammasiri. 2021. Large scale in vitro micropropagation of an ornamental plant, Oxalis triangularis A. St.-Hil, for commercial application. Acta Hortic. 1339. 245-256.
- Rojas, C., Martinez, J., and Perez, L. 2023. Occurrence of leaf rust disease in Oxalis triangularis caused by Puccinia oxalidis in Valdivia, Chile. Plant Disease 107(7): 2225-2235.
- Senyange, B., J. Kim, and S. Lee. 2025. Tissue engineering in the agri-food industry: current status, socio-economic overview and regulatory compliance. Current Opinion in Biotechnology 91(3): 103228-103239.
- Sharma, S., N. R. Bhuyan, and J. P. Mohanty. 2024. A pharmacognostic, phytochemical, and antioxidant potential of Oxalis triangularis. Journal of Applied Pharmaceutical Research 12(5): 133-145.
- Taha, R. M. T., H. El-Sayed, and M. Farag. 2013. Synthetic seeds production and regeneration of Oxalis triangularis for mass propagation and conservation. International Journal of Environmental Science and Development 4(5): 461-464.
- Taha, R., N. A. Hasbullah, and A. Awal. 2009. In vitro flowering of selected ornamental plants. In II International Conference on Landscape and Urban Horticulture 881(20): 141-146.
- Tarigan, L. 2025. Sterilization of Plant Tissue Culture Equipment. Journal of Innovation and Scientific Collaboration 1(1): 25-30.
- Teng, W. L., and Y. W. Ngai. 1999. Regeneration of Oxalis triangularis ssp. triangularis from suspension cells cultured in three different systems (solid, liquid-flask and bioreactor cultures). Plant Cell Reports 18(1): 701-706.
- Xiao, Y., W. Tuo, X. Wang, B. Feng, X. Xu, S. Ahmad, Y. Zhao, and S. Wu. 2023. Establishment of a Rapid and effective Agrobacterium-mediated genetic Transformation System of Oxalis triangularis ‘Purpurea’. Plants 12(24): 4130.
- Zhou, Y., L. Chen, J. Wang, and M. Zhang. 2023. High efficiency regeneration system from Blueberry Leaves and stems. Life 13(1): 242.
Öz
An in vitro propagation protocol of Oxalis triangularis A.st.-Hil, a valuable medicinal and ornamental plant, has been developed. In experimental studies, α-Naphthalene acetic acid (NAA) (0-0.5-1- 2 mg/L) and 6-Benzylaminopurine (BAP) (0.5-1 mg/L) plant growth regulators were applied together or alone to O. triangularis leaf and petiole explants. The best shoot formation per explant and the best leaf formation were obtained as 5.67 ± 2.43 and 4.00 ± 1.86 from leaf explants cultured in Murashige and Skoog (MS) medium containing 2 mg/L NAA and 0.5 mg/L BAP plant growth regulators, respectively. The shoots obtained were transferred to MS medium containing 0.5 mg/L NAA for root development. The plants obtained as a result of micropropagation processes were successfully acclimatized.
Anahtar Kelimeler
Plant tissue culture micropropagation auxin cytokinin Oxalis triangularis
Kaynakça
- Abdalla, N., K. Moustafa, M. Elsharkawy, and A. A. Al-Huqail. 2022. An academic and technical overview on plantmicropropagation challenges. Horticulturae 8(8): 677-688.
- Agnihotri, S., V. Rai, M. Tiwari, and D. Singh. 2025. Elicitation of the Himalayan toothache relieving tree (Zanthoxylum armatum DC.): enhancing secondary metabolites in vitro shoot cultures. Plant Cell, Tissue and Organ Culture 9(1): 7-12.
- Athari, M., A. Alizadeh, and S. Rahimi. 2024. Study on physiological and morphological traits of purple shamrock (Oxalis triangularis A. St.-Hil.) as affected by humic acid under salinity stress. Acta agriculturae Slovenica 120(1): 1-11.
- Babaoğlu, M., M. Yorgancılar, and M. A. Akbudak. 2001. Doku Kültürü: Temel laboratuar teknikleri. s. 1-35. In: Babaoğlu, M., Gürel, E., Özcan, S. (Eds.). Bitki biyoteknolojisi. Doku kültürü ve uygulamaları. Cilt I. Selçuk Üniversitesi Yayınları. Konya.
- Chen, S., L. Wang, J. Li, and Y. Zhang. 2024. Establishment of in vitro regeneration system for leaves and petioles of Oxalis triangularis 'Purpurea'. Technology in Horticulture 4(1): 11-19.
- Dupra, K. J. B., M. A. Aceret. 2024. Bioactivities of purple shamrock (Oxalis triangularis) crude extract and evaluation of shamrock topical antibacterial gel. International Journal of Integrated Science and Technology 1(2): 436-465.
- Gürel, A., S. Hayta, and E. Yılmaz. 2013. Bitki hücre, doku ve organ kültürü uygulamaları. Ege Üniversitesi Basımevi.
- Huh, S., J. Kim, S. Lee, and H. Park. 2010. Melanogenesis inhibitory effect of fatty acid alkyl esters isolated from Oxalis triangularis. Biological and Pharmaceutical Bulletin 33(7): 1242-1245.
- Joshi, N., R. Sharma, and A. Singh. 2024. Production of secondary metabolites from medicinal plants through tissue culture. In Secondary Metabolites and Biotherapeutics 7(1): 63-77.
- Kaviani, B. 2015. Some useful information about micropropagation. Journal of Ornamental Plants 5(1): 29-40.
- Klaocheed, S., S. Rittirat, S.Kalawong, and K. Thammasiri. 2024. Maximization of micropropagule production in an attractive ornamental plant, Oxalis triangularis A. St.-Hil. Trends in Sciences 21(8): 7917-7917.
- Kocaçalışkan, İ. 2021. Doku ve Hücre Kültürü Teknikleri (3. Basım). Nobel Akademik Yayıncılık.
- Luo, B., L. Chen, G. Chen, Y. Wang, Q. Xie, X. Chen, and Z. Hu. 2022. Transcription and metabolism pathways of anthocyanins in purple clover (Oxalis triangularis A. St.-Hil.). Metabolites 12(12): 1290-1307.
- Martin, K. 2002. Rapid propagation of Holostemma ada-kodien Schult., a rare medicinal plant, through axillary bud multiplication and indirect organogenesis. Plant Cell Reports, 21, 112-117.
- Murashige, T. 1974. Plant Propagation through Tissue Cultures. Annual Review of Plant Physiology 25: 135-166.
- Pamplona, R. S., C. D. Dela Cruz, and M. A. Tayangona. 2023. Selected Laboratory Equipment and Kitchen Utensils as an Alternative Sterilization Method for Sterilizing Plant Tissue Culture Media. bioRxiv 10(1): 1-22.
- Pan, H., L. Zhang, Y. Li, and Z. Wang. 2024. Establishment of callus induction and plantlet regeneration systems of Peucedanum praeruptorum Dunn based on the tissue culture method. Plant Methods 20(1): 174-190.
- Pasternak, T. P., and D. Steinmacher. 2024. Plant growth regulation in cell and tissue culture in vitro. Plants 13(2): 327-341.
- Pazmiño-Durán, E. A., P. Loyola, and F. Díaz. 2001. Anthocyanins from Oxalis triangularis as potential food colorants. Food Chemistry 75(2): 211-216.
- Prasuna, S., and B. Srinivas. 2015. In vitro regeneration studies of Oxalis corniculata from nodal explants. Int J Recent Sci Res 6: 6216-6220.
- Preil, W. 2003. Micropropagation of ornamental plants. In: Plant Tissue Culture: 100 Years Since Gottlieb Haberlandt pp. 115-133. Springer Vienna, Vienna.
- Ren, J., Q. Zhu, Y. Meng, F. Tang, Y. Xuan, D. Shi, S. Fu. 2017. Study on the Phenotypic Mutation Generated from 60Coγ-ray Irradiated Oxalias triangularis Purpurea Regeneration System. Agricultural Science & Technology 18(5): 756-762.
- Rittirat, S., S. Klaocheed, and K. Thammasiri. 2021. Large scale in vitro micropropagation of an ornamental plant, Oxalis triangularis A. St.-Hil, for commercial application. Acta Hortic. 1339. 245-256.
- Rojas, C., Martinez, J., and Perez, L. 2023. Occurrence of leaf rust disease in Oxalis triangularis caused by Puccinia oxalidis in Valdivia, Chile. Plant Disease 107(7): 2225-2235.
- Senyange, B., J. Kim, and S. Lee. 2025. Tissue engineering in the agri-food industry: current status, socio-economic overview and regulatory compliance. Current Opinion in Biotechnology 91(3): 103228-103239.
- Sharma, S., N. R. Bhuyan, and J. P. Mohanty. 2024. A pharmacognostic, phytochemical, and antioxidant potential of Oxalis triangularis. Journal of Applied Pharmaceutical Research 12(5): 133-145.
- Taha, R. M. T., H. El-Sayed, and M. Farag. 2013. Synthetic seeds production and regeneration of Oxalis triangularis for mass propagation and conservation. International Journal of Environmental Science and Development 4(5): 461-464.
- Taha, R., N. A. Hasbullah, and A. Awal. 2009. In vitro flowering of selected ornamental plants. In II International Conference on Landscape and Urban Horticulture 881(20): 141-146.
- Tarigan, L. 2025. Sterilization of Plant Tissue Culture Equipment. Journal of Innovation and Scientific Collaboration 1(1): 25-30.
- Teng, W. L., and Y. W. Ngai. 1999. Regeneration of Oxalis triangularis ssp. triangularis from suspension cells cultured in three different systems (solid, liquid-flask and bioreactor cultures). Plant Cell Reports 18(1): 701-706.
- Xiao, Y., W. Tuo, X. Wang, B. Feng, X. Xu, S. Ahmad, Y. Zhao, and S. Wu. 2023. Establishment of a Rapid and effective Agrobacterium-mediated genetic Transformation System of Oxalis triangularis ‘Purpurea’. Plants 12(24): 4130.
- Zhou, Y., L. Chen, J. Wang, and M. Zhang. 2023. High efficiency regeneration system from Blueberry Leaves and stems. Life 13(1): 242.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Bitki Biyoteknolojisi |
| Bölüm | Makaleler |
| Yazarlar | |
| Yayımlanma Tarihi | 30 Haziran 2025 |
| Gönderilme Tarihi | 3 Ocak 2025 |
| Kabul Tarihi | 19 Haziran 2025 |
| Yayımlandığı Sayı | Yıl 2025 |