Effect of Different Zinc Concentrations on Total Protein, Hydrogen Peroxide Content and Peroxidase Activity in Pepper (Capsicum annuum l.) Seedlings
Öz
In this study, the effect on differrent concentrations of zinc (ZnCl2) on peroxidase activity, total protein and hydrogen peroxide content in leaf of pepper (Capsicum annuum L.) seedlings were researched. 6-7 leafed seedlings were exposed to 1, 10 and 50 µM ZnCl2 for six days at 48 h intervals. The results of the present study showed that the physiological status of pepper was affected by Zn exposure. The highest total protein amount was detected in 50 µM ZnCl2 application, on the two day after the application in the leaves of KM-hot pepper (P<0,05). The lowest total protein content was detected in 50 µM ZnCl2 application, on the sixth day after the application (P<0,05). When compared to control, the maximum increase of peroxidase activity was determined in 10 ve 50 µM ZnCl2 applications, on the sixth day following the application (P<0,05). The changes of hydrogen peroxide amount in high zinc concentrations was observed (P<0,05). The highest hydrogen peroxide content was detected in 50 µM ZnCl2 application, on the sixth day after the application. In this study, it was observed that treatment of pepper with zinc led to an increased level of peroxidase in the leaf tissues. The activation of peroxidase could be probably associated with enhanced H2O2 production. The increased peroxidase activity could further stimulate the repair processes (lignification i.e) in heavy metal-stressed. This suggests that the plants had started adaptive processes to survive adverse conditions, and plants.
Anahtar Kelimeler
Kaynakça
- Bakardjieva N, Christov K (1996) Effect of calcium and zinc ions on the sensitivity of peroxidase from mosses (Mnium sp.) and ferns (Polydium vulgare) to hight temperature. Can J Bot 74: 1665-1670.
- Bekiaroglu P, Karataglis S (2002) The effect of lead and zinc on Mentha spicata. J Agron Crop Sci 188: 201-205.
- Bobak M (1985) Ultrastructure changes of the nucleus and its components in meristematic root cells of the horse-bean after zinc in toxication. Physiol Plants 15: 31-36.
- Bolwell GP, Bindschedler LV, Blee KA, Butt, VS, Davies, DR, Gardner SL, Gerrish C, Minibayeva F (2002) The apoplastic oxidative burst in response to biotic stress in plants: a tree component system. J Exp Bot 53: 1367– 1376.
- Bradford MM (1976) A rapid and sensitive for the quantitation of microgram quan- titites of protein utilizing the principle of protein–dye binding. Anal Biochem 72: 248–254.
- Burzynski M, Klobus G (2004) Changes of photosyntheticparameters in cucumber leaves under Cu, Cd and Pb stress.Photosynth 42(4): 505-510.
- Delledone M, Murgia MI, Ederle D, Sbicego PF, Biondian A, Polveraria A, Lamb C (2002) Reactive oxygen intermediates modulates nitric oxide signalling in the hypersensitive disease-resistance response. Plant Physiol Biochem 40: 605-610.
- Doganlar ZB, Cakmak S, Yanik T (2012) Metal Uptake and Physiological Changes in Lemna gibba Exposed to Manganese and Nickel. I J Biol 4(3): 148-157.
- Eraslan F, İnal A, Savaştürk O, Güneş A (2007) Changes in antioxidative system and membrane damage of lettuce in response to salinity and boron toxicity. Sci Hortic 114 (1): 5-10.
- Ermiş İ (2002) Bazı arpa çeşitlerinin çimlenme yüzdesi ve antioksidant enzim düzeylerine bor stresinin etkisi. Yüksek lisans tezi, Ege üniversitesi. Fen bilimleri enstitüsü, İzmir.
- Harinasuf P, Poonsopa D, Roengmogkol K, Charoensataporn R (2003) Salinity effects on antioxidant enzymes in mulberry cultivar. Sci Asia 29: 109-113.
- He CY, Hsiang T, Wolyn DJ (2002) Induction of systemic disease resistance and pathogen defence responses in Asparagus officinalis inoculated with nonpathogenic strains of Fusarium oxysporum. Plant Pathol 51: 225- 230.
- Kerby K, Somerville SC (1992) Purification of an infection-related, extracellular peroxidase from barley. Plant Physiol 100: 397-402.
- Khurana N, Chatterjee C (2001). Influence of variable zinc on yield, oil content, and physiology of sunflower. Commun Soil Sci Plant Anal 32: 3023-3030.
- Kırbağ F, Munzuroğlu O (2006) Toxic effects of cadmium (Cd++) on metabolism of sunflower (Helianthus annuus L.) seedlings. Acta Agric Scand, Section B - Soil and Plant Sci 56(3): 224-229.
- Kim KY, Kwon SY, Lee HS, Hur Y, Bang CW, Choi KS, Kwak SS (2000) Differantial expression of four sweet potato peroxidase genes in response to abscisic acid and ethaphon. Phytochem54: 19-22.
- Kurkela S, Franck M, Heino P, Long V, Palva ET (1988) Cold induced gene expression in Arabidopsis thaliana L. Plant Cell Rep. 7: 498– 598.
- Lin CC, Kao CH (2001) Cell wall peroxidase activity, hydrogen peroxide level and NaCl- inhibited root growth of rice seedlings. Plant and Soil 230: 135-143.
- Manivasagaperumal R, Balamurugan S, Thiyagarajan G, Sekar J (2011) Effect of Zinc on Germination, Seedling Growth and Biochemical Content of Cluster Bean (Cyamopsis tetragonoloba (L.) Taub). Curr Bot 2: 11-15.
- Mohammi M, Kazemi H (2002) Changes in peroxidase and polyphenol activity in susceptible and resistant wheat heads inoculated with Fusarium graminearum and – induced resistance. Plant Sci. 162: 491–498.
- Nepovim A, Podlipna R, Soudek P, Schröder P, Vanek T (2004) and nitroaromatic compounds on horseradish glutathione S-transferase and peroxidase. Chemosphere 57 (8): 1007-15.
- Patykowski J, Urbanek H (2003) Activity of enzymes related to H2O2 generation and metabolism in leaf apoplastic fraction of tomato leaves infected with Botrtis cinerea. J. Phytopatol. 151:153-161.
- Sinha S, Saxena R (2006) Effect of iron on lipid peroxidation,and enzymatic antioxidants and bacoside-A content in medicinal plant Bacopa monnieri L. Chemosphere, 62 , 1340–1350.Torres, M.A., 2010. ROS in biotic interactions. Physiol. Plant. 138: 414–429. and non
- Velikova V, Yordanov I, Edreva A (2000) Oxidative stres and some antioxidant systems in acid rain-treated bean plants. Protective role of exogenous polyamines. Plant Sci 151: 59-66.
- Zheng HZ, Cui CL, Zhang YT, Wang D, Jing Y, Kim YK (2005) Active changes of lignification- related enzymes in pepper response to Glomus intraradices and/ or Phytophthora capsici. J Zhejaiang Univ Sci 6(8):778-786.
Biber (Capsicum annuum L.) Fidelerinde Farklı Çinko Konsantrasyonlarının Total Protein, Hidrojen Peroksit İçeriği ve Peroksidaz Aktivitesi Üzerine Etkisi
Öz
Bu çalışmada biber (Capsicum annuum L.) fidelerinin yapraklarında peroksidaz aktivitesi, total protein ve hidrojen peroksit içeriğine çinko (ZnCl2)’un farklı konsantrasyonlarının etkisi araştırılmıştır. 6-7 yapraklı fideler 48 saat aralıkla altı gün süresince 1, 10 ve 50 µM ZnCl2 uygulamalarına maruz bırakılmıştır. Bu çalışmanın sonuçları biberin bazı fizyolojik olaylarının çinkodan etkilendiğini göstermiştir. Uygulamanın 2. gününde Km-Acı biber yapraklarında en yüksek total protein miktarı 50 µM ZnCl2 uygulamasında belirlenmiştir (P<0,05). Uygulamanın 6. gününde Km-Acı biber yapraklarında en düşük total protein miktarı 50 µM ZnCl2 uygulamasında belirlenmiştir (P<0,05). Uygulamanın 6. günde, kontrol grubu ile karşılaştırıldığında peroksidaz aktivitesindeki en fazla artış 10 ve 50 µM ZnCl2 uygulamalarında belirlenmiştir (P<0,05). Yüksek çinko konsantrasyonlarında hidrojen peroksit miktarında değişimler gözlenmiştir. En yüksek hidrojen peroksit içeriği uygulamanın 6. gününde 50 µM ZnCl2 uygulamasında belirlenmiştir. Bu çalışmada bibere uygulanan çinkonun, yaprak dokularında peroksidaz düzeyinde bir artışa yol açtığı gözlenmiştir. Peroksidazın aktivasyonu artan H2O2 üretimi ile bağlantılı olabilir. Artan peroksidaz aktivitesi ağır metal stresi altında onarım yollarını (lignifikasyon gibi) uyarabilir. Bu da, bitkilerin olumsuz koşullarda hayatta kalmak için adaptif yolları harekete geçirdiğini göstermektedir.
Anahtar Kelimeler
Kaynakça
- Bakardjieva N, Christov K (1996) Effect of calcium and zinc ions on the sensitivity of peroxidase from mosses (Mnium sp.) and ferns (Polydium vulgare) to hight temperature. Can J Bot 74: 1665-1670.
- Bekiaroglu P, Karataglis S (2002) The effect of lead and zinc on Mentha spicata. J Agron Crop Sci 188: 201-205.
- Bobak M (1985) Ultrastructure changes of the nucleus and its components in meristematic root cells of the horse-bean after zinc in toxication. Physiol Plants 15: 31-36.
- Bolwell GP, Bindschedler LV, Blee KA, Butt, VS, Davies, DR, Gardner SL, Gerrish C, Minibayeva F (2002) The apoplastic oxidative burst in response to biotic stress in plants: a tree component system. J Exp Bot 53: 1367– 1376.
- Bradford MM (1976) A rapid and sensitive for the quantitation of microgram quan- titites of protein utilizing the principle of protein–dye binding. Anal Biochem 72: 248–254.
- Burzynski M, Klobus G (2004) Changes of photosyntheticparameters in cucumber leaves under Cu, Cd and Pb stress.Photosynth 42(4): 505-510.
- Delledone M, Murgia MI, Ederle D, Sbicego PF, Biondian A, Polveraria A, Lamb C (2002) Reactive oxygen intermediates modulates nitric oxide signalling in the hypersensitive disease-resistance response. Plant Physiol Biochem 40: 605-610.
- Doganlar ZB, Cakmak S, Yanik T (2012) Metal Uptake and Physiological Changes in Lemna gibba Exposed to Manganese and Nickel. I J Biol 4(3): 148-157.
- Eraslan F, İnal A, Savaştürk O, Güneş A (2007) Changes in antioxidative system and membrane damage of lettuce in response to salinity and boron toxicity. Sci Hortic 114 (1): 5-10.
- Ermiş İ (2002) Bazı arpa çeşitlerinin çimlenme yüzdesi ve antioksidant enzim düzeylerine bor stresinin etkisi. Yüksek lisans tezi, Ege üniversitesi. Fen bilimleri enstitüsü, İzmir.
- Harinasuf P, Poonsopa D, Roengmogkol K, Charoensataporn R (2003) Salinity effects on antioxidant enzymes in mulberry cultivar. Sci Asia 29: 109-113.
- He CY, Hsiang T, Wolyn DJ (2002) Induction of systemic disease resistance and pathogen defence responses in Asparagus officinalis inoculated with nonpathogenic strains of Fusarium oxysporum. Plant Pathol 51: 225- 230.
- Kerby K, Somerville SC (1992) Purification of an infection-related, extracellular peroxidase from barley. Plant Physiol 100: 397-402.
- Khurana N, Chatterjee C (2001). Influence of variable zinc on yield, oil content, and physiology of sunflower. Commun Soil Sci Plant Anal 32: 3023-3030.
- Kırbağ F, Munzuroğlu O (2006) Toxic effects of cadmium (Cd++) on metabolism of sunflower (Helianthus annuus L.) seedlings. Acta Agric Scand, Section B - Soil and Plant Sci 56(3): 224-229.
- Kim KY, Kwon SY, Lee HS, Hur Y, Bang CW, Choi KS, Kwak SS (2000) Differantial expression of four sweet potato peroxidase genes in response to abscisic acid and ethaphon. Phytochem54: 19-22.
- Kurkela S, Franck M, Heino P, Long V, Palva ET (1988) Cold induced gene expression in Arabidopsis thaliana L. Plant Cell Rep. 7: 498– 598.
- Lin CC, Kao CH (2001) Cell wall peroxidase activity, hydrogen peroxide level and NaCl- inhibited root growth of rice seedlings. Plant and Soil 230: 135-143.
- Manivasagaperumal R, Balamurugan S, Thiyagarajan G, Sekar J (2011) Effect of Zinc on Germination, Seedling Growth and Biochemical Content of Cluster Bean (Cyamopsis tetragonoloba (L.) Taub). Curr Bot 2: 11-15.
- Mohammi M, Kazemi H (2002) Changes in peroxidase and polyphenol activity in susceptible and resistant wheat heads inoculated with Fusarium graminearum and – induced resistance. Plant Sci. 162: 491–498.
- Nepovim A, Podlipna R, Soudek P, Schröder P, Vanek T (2004) and nitroaromatic compounds on horseradish glutathione S-transferase and peroxidase. Chemosphere 57 (8): 1007-15.
- Patykowski J, Urbanek H (2003) Activity of enzymes related to H2O2 generation and metabolism in leaf apoplastic fraction of tomato leaves infected with Botrtis cinerea. J. Phytopatol. 151:153-161.
- Sinha S, Saxena R (2006) Effect of iron on lipid peroxidation,and enzymatic antioxidants and bacoside-A content in medicinal plant Bacopa monnieri L. Chemosphere, 62 , 1340–1350.Torres, M.A., 2010. ROS in biotic interactions. Physiol. Plant. 138: 414–429. and non
- Velikova V, Yordanov I, Edreva A (2000) Oxidative stres and some antioxidant systems in acid rain-treated bean plants. Protective role of exogenous polyamines. Plant Sci 151: 59-66.
- Zheng HZ, Cui CL, Zhang YT, Wang D, Jing Y, Kim YK (2005) Active changes of lignification- related enzymes in pepper response to Glomus intraradices and/ or Phytophthora capsici. J Zhejaiang Univ Sci 6(8):778-786.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Mimarlık |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Yayımlanma Tarihi | 9 Nisan 2012 |
| Yayımlandığı Sayı | Yıl 2012 Cilt: 13 Sayı: 2 |
Kaynak Göster
Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi Creative Commons Alıntı 4.0 Uluslararası Lisansı ile lisanslanmıştır.