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Inactivation of Bacillus subtilis and Candida tropicalis by Wisteria sinensis maceration oil

Yıl 2021, Cilt: 22 Sayı: 1, 97 - 102, 12.05.2021

Elif Eliuz , Yusuf Sıcak

https://doi.org/10.17474/artvinofd.776142

Öz

In this study, the antimicrobial effect of maceration oil obtained from W. sinensis flowers oil soaked in olive oil on B. subtilis and C. tropicalis was investigated. In addition, the efficacy of W. sinensis maceration oil on inactivation of the strains of B. subtilis and C. tropicalis inoculated bulgur (pounded wheat) was investigated using dip incubation method. The components of W. sinensis macerate were analyzed by GC-MS and found the main components as olealdehyde (38.03%), oleic acid (29.13%), 9-octadecenoic acid (15.09%), (Z)-9,17-octadecadienal (7.87%) and palmitic acid (5.97%). Broth Microdilution and Agar Well Diffusion Method for antimicrobial activity of W. sinensis and also Modified TDtest for persistent/tolerant levels of microorganisms were used. Minimum Inhibitory Concentrations (MICs) of W. sinensis were 10.3 mg/mL and 9.6 mg/mL for B. subtilis and C. tropicalis while the inhibition zones were 2.23 mm and 2.07 mm, respectively. In TDTest which was made persistent/tolerant screening of microorganisms in W. sinensis condition, both of microrganisms were persistent sensitive. W. sinensis at 50 µL, 100 µL and 150 µL caused an almost 2-log reduction on the number of B. subtilis and C. tropicalis on bulgur.

Anahtar Kelimeler

W sinensis macerate B. subtilis C. tropicalis Bulgur Antimicrobial

Kaynakça

  • Andjelkovic M, Acun S, Van Hoed V, Verhé R, Van Camp J (2009) Chemical composition of Turkish olive oil - Ayvalik. JAOCS, J Am Oil Chem Soc 86:135-140.
  • Chen Z, He B, Zhou J, He D, Deng J, Zeng R (2016) Chemical compositions and antibacterial activities of essential oils extracted from Alpinia guilinensis against selected foodborne pathogens. Ind Crops Prod 83: 607-613.
  • Cook RTA, Denton JO, Denton G (2015) Pathology of oakwisteria powdery mildew. Fungal Biol 119:657-671.
  • Çetinkaya A (2019) Chemical and microbiological properties of kavut flour produced in some regions of Turkey. Int J Food Nutr Res 2:1-8.
  • Erdogan Eliuz EA (2020) The Antimicrobial Activity of Combinations with Olive oil (Olea europaea L.) of Fatty Acids of Some Medicinal Plants Against Some Pathogens. J Microbiol Biotechnol Food Sci 9: 941-945.
  • Gefen O, Chekol B, Strahilevitz J, Balaban NQ (2017) TDtest:Easy detection of bacterial tolerance and persistence in clinical isolates by a modified disk-diffusion assay. Sci Rep 7: 41284.
  • Hammer KA., Carson CF, Rıleya TV (2012) Effects of Melaleuca alternifolia (tea tree) essential oil and the major monoterpene component terpinen-4-ol on the development of single- and multistep antibiotic resistance and antimicrobial susceptibility. Antimicrob Agents Chemother 56: 909-15.
  • Jiang YF, Chen XL, Lin H, Wang F, Chen F (2011) Floral Scent in Wisteria: Chemical composition, emission pattern, and regulation. J Am Soc Hortic Sci 136:307-314.
  • Kon KV, Ra MK (2012) Plant essential oils and their constituents in coping with multidrug-resistant bacteria. Expert Rev of Anti-infe 10: 775-90
  • Li Y, Deng C, Qiao Y, Zhao X, Zhou Q (2017) Characterization of a new badnavirus from Wisteria sinensis. Arch Virol 162:2125-2129.
  • Logan NA. (2012) Bacillus and relatives in foodborne illness. J Appl Microbiol 113:417-429.
  • Nascimento GGF, Locatelli J, Freitas PC, Silva GL (2000) Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Brazilian J Microbiol 31: 247-256.
  • Preethi R, Devanathan VV, Loganathan M (2010) Antimicrobial and Antioxidant Efficacy of Some Medicinal Plants Against Food Borne Pathogens. Adv Biol Res (Rennes) 4:122-125.
  • Sıcak Y, Erdoğan Eliuz EA (2019) Chemical content and biological activity spectrum of Nigella sativa seed oil. KSU J Agric Nat 22: 928-934.
  • Yabalak E (2018) Radical scavenging activity and chemical composition of methanolic extract from Arum dioscoridis Sm. var. dioscoridis and determination of its mineral and trace elements. J Turkish Chem Soc Sect A Chem 5: 205-218.
  • Yurdakul ÖK, Keyvan E, Kırdar SS. Etsiz Çiğ Köftelerde Patojen Candida spp. Varlığının Tespiti. Etlik Vet Mikrobiyoloji Derg 2017. 28: 46-50.

Willeria sinensis maserasyon yağı ile Bacillus subtilis ve Candida tropicalis inaktivasyonu

Yıl 2021, Cilt: 22 Sayı: 1, 97 - 102, 12.05.2021

Elif Eliuz , Yusuf Sıcak

https://doi.org/10.17474/artvinofd.776142

Öz

Bu çalışmada, zeytinyağına batırılmış W. sinensis çiçeklerinden elde edilen maserasyon yağının B. subtilis ve C. tropicalis üzerindeki antimikrobiyal etkisi araştırıldı. Ek olarak, W. sinensis maserasyon yağının, bulgura (dövülmüş buğday) aşılanmış B. subtilis ve C. tropicalis suşlarının inaktivasyonu üzerindeki etkinliği daldırma inkübasyon metodu kullanılarak araştırıldı. W. sinensis maserat bileşenleri GC-MS ile analiz edildi ve ana bileşenleri olealdehit (%38.03), oleik asit (%29.13), (Z)-9,17-oktadekadienal asit (%15.09), (Z)-9,17-oktadekadienal (%7.87) ve palmitik asit (%5.97) olarak bulundu. W. sinensis'in antimikrobiyal aktivitesi için Sıvı Mikrodilüsyon ve Agar Kuyucuk Difüzyon Yöntemi ve ayrıca kalıcı/toleranslı mikroorganizma seviyeleri için Modifiye TDtest kullanıldı. W sinensis’in Minimum İnhibitör Konsantrasyonları (MİK), B. subtilis ve C. tropicalis için 10.3 mg/mL ve 9.6 mg/mL iken inhibisyon zonları sırasıyla 2.23 mm ve 2.07 mm idi. W. sinensis varlığında mikroorganizmaların kalıcı/toleranslı taranması yapılan TDTest'te her iki mikroorganizmanın da kalıcı duyarlılığı saptandı. W. sinensis’in 50 µL, 100 µL ve 150 µL’si, bulgur üzerindeki B. subtilis ve C. tropicalis sayısında neredeyse 2 log azalmaya neden oldu.

Anahtar Kelimeler

B. subtilis Bulgur W sinensis maseratı C. tropicalis Antimikrobiyal

Kaynakça

  • Andjelkovic M, Acun S, Van Hoed V, Verhé R, Van Camp J (2009) Chemical composition of Turkish olive oil - Ayvalik. JAOCS, J Am Oil Chem Soc 86:135-140.
  • Chen Z, He B, Zhou J, He D, Deng J, Zeng R (2016) Chemical compositions and antibacterial activities of essential oils extracted from Alpinia guilinensis against selected foodborne pathogens. Ind Crops Prod 83: 607-613.
  • Cook RTA, Denton JO, Denton G (2015) Pathology of oakwisteria powdery mildew. Fungal Biol 119:657-671.
  • Çetinkaya A (2019) Chemical and microbiological properties of kavut flour produced in some regions of Turkey. Int J Food Nutr Res 2:1-8.
  • Erdogan Eliuz EA (2020) The Antimicrobial Activity of Combinations with Olive oil (Olea europaea L.) of Fatty Acids of Some Medicinal Plants Against Some Pathogens. J Microbiol Biotechnol Food Sci 9: 941-945.
  • Gefen O, Chekol B, Strahilevitz J, Balaban NQ (2017) TDtest:Easy detection of bacterial tolerance and persistence in clinical isolates by a modified disk-diffusion assay. Sci Rep 7: 41284.
  • Hammer KA., Carson CF, Rıleya TV (2012) Effects of Melaleuca alternifolia (tea tree) essential oil and the major monoterpene component terpinen-4-ol on the development of single- and multistep antibiotic resistance and antimicrobial susceptibility. Antimicrob Agents Chemother 56: 909-15.
  • Jiang YF, Chen XL, Lin H, Wang F, Chen F (2011) Floral Scent in Wisteria: Chemical composition, emission pattern, and regulation. J Am Soc Hortic Sci 136:307-314.
  • Kon KV, Ra MK (2012) Plant essential oils and their constituents in coping with multidrug-resistant bacteria. Expert Rev of Anti-infe 10: 775-90
  • Li Y, Deng C, Qiao Y, Zhao X, Zhou Q (2017) Characterization of a new badnavirus from Wisteria sinensis. Arch Virol 162:2125-2129.
  • Logan NA. (2012) Bacillus and relatives in foodborne illness. J Appl Microbiol 113:417-429.
  • Nascimento GGF, Locatelli J, Freitas PC, Silva GL (2000) Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Brazilian J Microbiol 31: 247-256.
  • Preethi R, Devanathan VV, Loganathan M (2010) Antimicrobial and Antioxidant Efficacy of Some Medicinal Plants Against Food Borne Pathogens. Adv Biol Res (Rennes) 4:122-125.
  • Sıcak Y, Erdoğan Eliuz EA (2019) Chemical content and biological activity spectrum of Nigella sativa seed oil. KSU J Agric Nat 22: 928-934.
  • Yabalak E (2018) Radical scavenging activity and chemical composition of methanolic extract from Arum dioscoridis Sm. var. dioscoridis and determination of its mineral and trace elements. J Turkish Chem Soc Sect A Chem 5: 205-218.
  • Yurdakul ÖK, Keyvan E, Kırdar SS. Etsiz Çiğ Köftelerde Patojen Candida spp. Varlığının Tespiti. Etlik Vet Mikrobiyoloji Derg 2017. 28: 46-50.
Toplam 16 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Yapısal Biyoloji
Bölüm Araştırma Makalesi
Yazarlar

Elif Eliuz 0000-0003-4317-3000

Yusuf Sıcak 0000-0003-2339-5837

Yayımlanma Tarihi 12 Mayıs 2021
Kabul Tarihi 7 Nisan 2021
Yayımlandığı Sayı Yıl 2021 Cilt: 22 Sayı: 1

Kaynak Göster

APA Eliuz, E., & Sıcak, Y. (2021). Inactivation of Bacillus subtilis and Candida tropicalis by Wisteria sinensis maceration oil. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, 22(1), 97-102. https://doi.org/10.17474/artvinofd.776142
AMA Eliuz E, Sıcak Y. Inactivation of Bacillus subtilis and Candida tropicalis by Wisteria sinensis maceration oil. AÇÜOFD. Mayıs 2021;22(1):97-102. doi:10.17474/artvinofd.776142
Chicago Eliuz, Elif, ve Yusuf Sıcak. “Inactivation of Bacillus Subtilis and Candida Tropicalis by Wisteria Sinensis Maceration Oil”. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi 22, sy. 1 (Mayıs 2021): 97-102. https://doi.org/10.17474/artvinofd.776142.
EndNote Eliuz E, Sıcak Y (01 Mayıs 2021) Inactivation of Bacillus subtilis and Candida tropicalis by Wisteria sinensis maceration oil. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi 22 1 97–102.
IEEE E. Eliuz ve Y. Sıcak, “Inactivation of Bacillus subtilis and Candida tropicalis by Wisteria sinensis maceration oil”, AÇÜOFD, c. 22, sy. 1, ss. 97–102, 2021, doi: 10.17474/artvinofd.776142.
ISNAD Eliuz, Elif - Sıcak, Yusuf. “Inactivation of Bacillus Subtilis and Candida Tropicalis by Wisteria Sinensis Maceration Oil”. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi 22/1 (Mayıs 2021), 97-102. https://doi.org/10.17474/artvinofd.776142.
JAMA Eliuz E, Sıcak Y. Inactivation of Bacillus subtilis and Candida tropicalis by Wisteria sinensis maceration oil. AÇÜOFD. 2021;22:97–102.
MLA Eliuz, Elif ve Yusuf Sıcak. “Inactivation of Bacillus Subtilis and Candida Tropicalis by Wisteria Sinensis Maceration Oil”. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, c. 22, sy. 1, 2021, ss. 97-102, doi:10.17474/artvinofd.776142.
Vancouver Eliuz E, Sıcak Y. Inactivation of Bacillus subtilis and Candida tropicalis by Wisteria sinensis maceration oil. AÇÜOFD. 2021;22(1):97-102.
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