Öz
Amaç: Kronik spontan ürtikerli olguların bir kısmında etiyopatogenezden mast hücreleri ve bazofillerin yüzeyindeki yüksek afiniteli IgE reseptörüne
veya IgE’ye karşı oluşan otoantikorlar sorumlu tutulmaktadır. Otoimmünitenin gelişmesine neden olan faktörler bilinmemektedir. Genetik faktörlerin
otoimmünitede rol oynadığı bilinmektedir ve insan lökosit antijeni (HLA) alellerinin birçok otoimmün hastalıkla ilişkili olduğu gösterilmiştir.
Çalışmamızda kronik spontan ürtikerli hastalarda HLA klas II alellerinin sıklığının araştırılması amaçlanmıştır.
Gereç ve Yöntem: Kesitsel tipteki bu çalışmada, 80 kronik spontan ürtikerli olguda ve 100 renal transplant donöründen oluşan kontrol grubunda
HLA klas II alelleri değerlendirildi. Olgu grubu otolog serum deri testi (OSDT) uygulanarak alt gruplara ayrıldı. HLA klas II alelleri, OSDT testi pozitif
ve negatif olan kronik spontan ürtiker olgularında ve kontrol grubunda moleküler analiz yöntemiyle (Olerup SSP DQ-DR Combi tray, Genovision,
Viyana, Avusturya) belirlendi.
Bulgular: OSDT hastaların %57,5’inde pozitif bulundu. Kronik spontan ürtikerli hastalarda HLA DRB1*04 ve HLA DQB1*08 alellerinin sıklığı, kontrol
grubuna göre anlamlı olarak daha yüksek tespit edildi. HLA DRB1*07 ve HLA DQB1*09 alellerinin sıklığı ise anlamlı derecede daha düşüktü (p<0,05).
OSDT pozitif ve negatif olgu grupları ile kontrol grubu karşılaştırıldığında; HLA DRB1*04 ve HLA DQB1*08 alelleri OSDT pozitif hasta grubunda daha
sık bulundu (p<0,05). OSDT negatif hasta grubunda ise HLA DRB1*01 ve HLA DRB1*07 alelleri düşük sıklıkta saptandı (p<0,05).
Sonuç: OSDT pozitif olan olgularda HLA DRB1*04 ve DQB1*08 alellerinin anlamlı derecede daha sık görülmesi ve bu alellerin OSDT negatif olan
olgularda kontrol grubundan anlamlı bir farklılık göstermemesi, hastaların bir kısmında patogenezden otoimmün mekanizmaların sorumlu olduğu
fikrini desteklemektedir
Anahtar Kelimeler
Genetik Yatkınlık HLA Klas II Alelleri Kronik Spontan Ürtiker Otolog Serum Deri Testi Otoimmünite
Etik Beyan
Ethics Committee Approval: This manuscript is derived from the thesis conducted in 2005. For this reason, ethical committee approval is not available Informed Consent: Patients were informed and consent was taken. Peer-review: Externally peer-reviewed.
Destekleyen Kurum
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Proje Numarası
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Teşekkür
-
Kaynakça
- 1. Kozel MM, Sabroe RA. Chronic urticaria: aetiology, management and current and future treatment options. Drugs. 2004;64:2515-2536.
- 2. Zuberbier T, Aberer W, Asero R, et al. The EAACI/GA²LEN/EDF/WAO guideline for the definition, classification, diagnosis and management of urticaria. Allergy. 2018;73:1393-1414.
- 3. Nettis E, Foti C, Ambrifi M, et al. Urticaria: recommendations from the Italian Society of Allergology, Asthma and Clinical Immunology and the Italian Society of Allergological, Occupational and Environmental Dermatology. Clin Mol Allergy. 2020;18:8.
- 4. Kaplan AP, Greaves M. Pathogenesis of chronic urticaria. Clin Exp Allergy. 2009;39:777-787.
- 5. Saini SS, Kaplan AP. Chronic Spontaneous Urticaria: The Devil’s Itch. J Allergy Clin Immunol Pract. 2018;6:1097-1106.
- 6. Grattan CE, Wallington TB, Warin RP, et al. A serological mediator in chronicidiopathic urticaria--a clinical, immunological and histological evaluation. Br J Dermatol. 1986;114:583-590.
- 7. Greaves M. Chronic urticaria. J Allergy Clin Immunol. 2000;105:664-672.
- 8. Greaves MW, Tan KT. Chronic urticaria: recent advances. Clin Rev Allergy Immunol. 2007;33:134-143.
- 9. Simmonds MJ, Gough SC. Genetic insights into disease mechanisms of autoimmunity. Br Med Bull. 2005;71:93-113.
- 10. Sabroe RA, Grattan CE, Francis DM, et al. The autologous serum skin test: a screening test for autoantibodi
- 11. Grattan CE. Autoimmune urticaria. Immunol Allergy Clin North Am. 2004;24:163-181.
- 12. Nettis E, Dambra P, D’Oronzio L, et al. Reactivity to autologous serum skin test and clinical features in chronic idiopathic urticaria. Clin Exp Dermatol. 2002;27:29-31.
- 13. Nettis E, Dambra P, D’Oronzio L, et al. Reactivity to autologous serum skin test and clinical features in chronic idiopathic urticaria. Clin Exp Dermatol. 2002;27:29-31.
- 14. Mete N, Gulbahar O, Aydin A, et al. Low B12 levels in chronic idiopathic urticaria. J Investig Allergol Clin Immunol. 2004;14:292-299.
- 15. Abul KA, Lichtman AH, Pober JS, editors. Self-tolerance and autoimmunity. In: Cellular and Molecular Immunology.Second edition, Philadelphia: WB. Saunders; 1994. s. 388-390.
- 16. van Neste D, Bouillenne C. HLA antigens and urticaria. Arch Dermatol Res. 1978;261:213-215.
- 17. Bozek A, Krajewska J, Filipowska B, et al. HLA status in patients with chronic spontaneous urticaria. Int Arch Allergy Immunol. 2010;153:419-423.
- 18. Aydogan K, Karadogan SK, Akdag I, et al. HLA class I and class II antigens in Turkish patients with chronic ordinary urticaria. Clin Exp Dermatol. 2006;31:424-429.
- 19. Doğan N, Çildağ S, Yenisey Ç, et al. The association between chronic spontaneous urticaria and HLA class I and class II antigen. Turk J Med Sci. 2020;50:1231-1235.
- 20. Coban M, Erdem T, Ozdemir S, et al. HLA class I and class II genotyping in patients with chronic urticaria. Int Arch Allergy Immunol. 2008;147:135- 139.
- 21. O’Donnell BF, O’Neill CM, Francis DM, et al. Human leucocyte antigen class II associations in chronic idiopathic urticaria. Br J Dermatol. 1999;140:853- 858.
- 22. Oztas P, Onder M, Gonen S, et al. Is there any relationship between human leucocyte antigen class II and chronic urticaria? (chronic urticaria and HLA class II). Yonsei Med J. 2004;45:392-395.
- 23. Chen J, Tan Z, Li J, et al. Association of HLA-DRB1, DQB1 alleles with chronic urticaria. J Huazhong Univ Sci Technolog Med Sci. 2005;25:354-356.
- 24. Calamita Z, Pelá AB, Gamberini M, et al. HLA among Brazilian patients with spontaneous chronic urticaria and positive autologous serum skin test. An Bras Dermatol. 2012;87:578-583.
Öz
Objectives: Autoantibodies against high-affinity IgE receptor or IgE on the surface of mast cells and basophils are held responsible for
etiopathogenesis in some cases with chronic spontaneous urticaria. Factors causing the development of autoimmunity are unknown. Genetic factors
are known to play a role in autoimmunity and human leukocyte antigen (HLA) alleles are associated with many autoimmune diseases. Our study
aimed to investigate the frequency of HLA class II alleles in patients with chronic spontaneous urticaria.
Materials and Methods: In this cross-sectional study, HLA class II alleles were evaluated in the 80 cases with chronic spontaneous urticaria and
in the control group consisting of 100 renal transplant donors. The case group was divided into subgroups by applying autologous serum skin
test (ASST). HLA class II alleles were determined in chronic spontaneous urticaria cases with positive and negative ASST and the control group by
molecular analysis method (Olerup SSP DQ-DR Combi tray, Genovision, Vienna, Austria).
Results: ASST was found positive in 57.5% of the patients. The frequency of HLA DRB1*04 and HLA DQB1*08 alleles was found to be significantly
higher in patients with chronic spontaneous urticaria compared to the control group. In contrast, the frequency of HLA DRB1*07 and HLA DQB1*09
alleles was significantly lower (p<0.05). When the ASST positive and negative case groups and the control group were compared, HLA DRB1*04 and
HLA DQB1*08 alleles were higher in the ASST positive patient group (p<0.05). In the ASST negative patient group, HLA DRB1*01 and HLA DRB1*07
alleles were found in low frequency (p<0.05).
Conclusion: The fact that there is a significantly higher frequency of HLA DRB1*04 and DQB1*08 alleles in cases with positive ASST and these alleles
do not differ significantly from the control group in cases with negative ASST support the idea that autoimmune mechanisms are responsible for
pathogenesis in some of the patients.
Anahtar Kelimeler
Genetic Predisposition HLA Class II Alleles Chronic Spontaneous Urticaria Autologous Serum Skin Test Autoimmunity
Proje Numarası
-
Kaynakça
- 1. Kozel MM, Sabroe RA. Chronic urticaria: aetiology, management and current and future treatment options. Drugs. 2004;64:2515-2536.
- 2. Zuberbier T, Aberer W, Asero R, et al. The EAACI/GA²LEN/EDF/WAO guideline for the definition, classification, diagnosis and management of urticaria. Allergy. 2018;73:1393-1414.
- 3. Nettis E, Foti C, Ambrifi M, et al. Urticaria: recommendations from the Italian Society of Allergology, Asthma and Clinical Immunology and the Italian Society of Allergological, Occupational and Environmental Dermatology. Clin Mol Allergy. 2020;18:8.
- 4. Kaplan AP, Greaves M. Pathogenesis of chronic urticaria. Clin Exp Allergy. 2009;39:777-787.
- 5. Saini SS, Kaplan AP. Chronic Spontaneous Urticaria: The Devil’s Itch. J Allergy Clin Immunol Pract. 2018;6:1097-1106.
- 6. Grattan CE, Wallington TB, Warin RP, et al. A serological mediator in chronicidiopathic urticaria--a clinical, immunological and histological evaluation. Br J Dermatol. 1986;114:583-590.
- 7. Greaves M. Chronic urticaria. J Allergy Clin Immunol. 2000;105:664-672.
- 8. Greaves MW, Tan KT. Chronic urticaria: recent advances. Clin Rev Allergy Immunol. 2007;33:134-143.
- 9. Simmonds MJ, Gough SC. Genetic insights into disease mechanisms of autoimmunity. Br Med Bull. 2005;71:93-113.
- 10. Sabroe RA, Grattan CE, Francis DM, et al. The autologous serum skin test: a screening test for autoantibodi
- 11. Grattan CE. Autoimmune urticaria. Immunol Allergy Clin North Am. 2004;24:163-181.
- 12. Nettis E, Dambra P, D’Oronzio L, et al. Reactivity to autologous serum skin test and clinical features in chronic idiopathic urticaria. Clin Exp Dermatol. 2002;27:29-31.
- 13. Nettis E, Dambra P, D’Oronzio L, et al. Reactivity to autologous serum skin test and clinical features in chronic idiopathic urticaria. Clin Exp Dermatol. 2002;27:29-31.
- 14. Mete N, Gulbahar O, Aydin A, et al. Low B12 levels in chronic idiopathic urticaria. J Investig Allergol Clin Immunol. 2004;14:292-299.
- 15. Abul KA, Lichtman AH, Pober JS, editors. Self-tolerance and autoimmunity. In: Cellular and Molecular Immunology.Second edition, Philadelphia: WB. Saunders; 1994. s. 388-390.
- 16. van Neste D, Bouillenne C. HLA antigens and urticaria. Arch Dermatol Res. 1978;261:213-215.
- 17. Bozek A, Krajewska J, Filipowska B, et al. HLA status in patients with chronic spontaneous urticaria. Int Arch Allergy Immunol. 2010;153:419-423.
- 18. Aydogan K, Karadogan SK, Akdag I, et al. HLA class I and class II antigens in Turkish patients with chronic ordinary urticaria. Clin Exp Dermatol. 2006;31:424-429.
- 19. Doğan N, Çildağ S, Yenisey Ç, et al. The association between chronic spontaneous urticaria and HLA class I and class II antigen. Turk J Med Sci. 2020;50:1231-1235.
- 20. Coban M, Erdem T, Ozdemir S, et al. HLA class I and class II genotyping in patients with chronic urticaria. Int Arch Allergy Immunol. 2008;147:135- 139.
- 21. O’Donnell BF, O’Neill CM, Francis DM, et al. Human leucocyte antigen class II associations in chronic idiopathic urticaria. Br J Dermatol. 1999;140:853- 858.
- 22. Oztas P, Onder M, Gonen S, et al. Is there any relationship between human leucocyte antigen class II and chronic urticaria? (chronic urticaria and HLA class II). Yonsei Med J. 2004;45:392-395.
- 23. Chen J, Tan Z, Li J, et al. Association of HLA-DRB1, DQB1 alleles with chronic urticaria. J Huazhong Univ Sci Technolog Med Sci. 2005;25:354-356.
- 24. Calamita Z, Pelá AB, Gamberini M, et al. HLA among Brazilian patients with spontaneous chronic urticaria and positive autologous serum skin test. An Bras Dermatol. 2012;87:578-583.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Dermatoloji |
| Bölüm | Makaleler |
| Yazarlar | |
| Proje Numarası | - |
| Yayımlanma Tarihi | 30 Haziran 2022 |
| Yayımlandığı Sayı | Yıl 2022 Cilt: 75 Sayı: 1 |
