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İnsan Lösemi Monosit Hücrelerinin Forbol-12-Miristat-13-Asetat ile Makrofaj Benzeri Hücrelere Farklılaşması

Yıl 2019, Cilt: 72 Sayı: 1, 34 - 38, 13.06.2019

Derya Biriken

Öz

Proje Numarası

-

Kaynakça

  • 1. Janeway CA, Travers P, Walport M, et al. Immunobiology, The Immune System In Health and Disease. 5th Edition, Elsevier Science Ltd/Gardland; 2001.
  • 2. Gordon S. Mononuclear phagocytes in immune defence. In: Immunology, Seventh Edition, Ed.: Male D, Brostoff J, Roth DB. Roitt, USA: Mosby Elsevier 2006;181-202.
  • 3. Smythies LE, Sellers M, Clements RH, et al. Human intestinal macrophages display profound inflammatory anergy despite avid phagocytic and bacteriocidal activity. J Clin Invest. 2005;115:66-75.
  • 4. Delves PJ, Martin SJ, Burton DR, et al. Innate immunity, In: Roitt’s Essential Immunology. 11th ed. Blackwell Publishing, USA 2006;1-20.
  • 5. Si-Tahar M, Touqui L, Chignard M. Innate immunity and inflammation- -two facets of the same anti-infectious reaction. Clin Exp Immunol. 2009;156:194-198.
  • 6. Tsuchiya S, Yamabe M, Yamagochi Y, et al. Establishment and characterization of a human acute monocytic leukemia cell line (THP-1). Int J Cancer. 1980;26:171-176.
  • 7. Chanput W, Mes JJ, Wichers HJ. THP-1 cell line: an in vitro cell model for immune modulation approach. Int Immunopharmacol. 2014;23:37-45.
  • 8. Qin Z. The use of THP-1 cells as a model for mimicking the function and regulation of monocytes and macrophages in the vasculature. Atherosclerosis. 2012;221:2-11.
  • 9. Solberg R, Smith R, Almlof M, et al. Legumain expression, activity and secretion are increased during monocyte-to-macrophage differentiation and inhibited by atorvastatin. Biol Chem 2015;396: 71-80.
  • 10. Auwerx J. The human leukemia cell line, THP-1: a multifacetted model for the study of monocyte-macrophage differentiation. Experientia. 1991;47:22-31.
  • 11. Tominaga T, Suzuki M, Saeki H, et al. Establishment of an activated macrophage cell line, A-THP-1, and its properties. Tohoku J Exp Med. 1998;186:99-119.
  • 12. Schwende H, Fitzke E, Ambs P, et al. Differences in the state of differentiation of THP-1 cells induced by phorbol ester and 1,25-dihydroxyvitamin D3. J Leukoc Biol. 1996;59:555-561.
  • 13. Bodet C, Chandad F, Greiner D. Modulation of cytokine production by Porphyromonas gingivalis in a macrophage and epithelial cell co-culture model. Microbes Infect. 2005;7:448-456.
  • 14. Shiratori H, Feinweber C, Luckhardt S, et al. THP-1 and human peripheral blood mononuclear cell-derived macrophages differ in their capacity to polarize in vitro. Mol Immunol. 2017;88:58-68.
  • 15. Kohro T, Tanaka T, Murakami T, et al. A comparison of differences in the gene expression profiles of phorbol 12-myristate 13-acetate differentiated THP- 1 cells and human monocyte-derived macrophage. J Atheroscler Thromb. 2004;11:88-97.
  • 16. Zhao W, Sun Z, Wang, S, et al. Wnt1 Participates in Inflammation Induced by Lipopolysaccharide Through Upregulating Scavenger Receptor A and NFkB. Inflammation. 2015;38:1700-1706.
  • 17. Park EK, Jung HS, Yang HI, et al. Optimized THP-1 differentiation is required for the detection of responses to weak stimuli. Inflamm Res. 2007;56:45-50.
  • 18. Freshney RI. Culture of Animal Cells: A Manual of Basic Technique. 3rd edition; Wiley-Liss, New York, 2000.
  • 19. Hjort MR, Brenyo AJ, Finkelstein JN, et al. Alveolar epithelial cellmacrophage interactions affect oxygen-stimulated interleukin-8 release. Inflammation. 2003;27:137-145.
  • 20. Lund ME, To J, O’Brien BA, et al. The choice of phorbol 12-myristate 13-acetate differentiation protocol influences the response of THP- 1 macrophages to a pro-inflammatory stimulus. J Immunol Methods. 2016;430:64-70.
  • 21. Aldo PB, Craveiro V, Guller S, et al. Effect of culture conditions on the phenotype of THP-1 monocyte cell line. Am J Reprod Immunol. 2013;70:80-86.
  • 22. Salin DB, Albayrak N, Yıldız S, et al. [Investigation of bactericidal effect and nitric oxide responses of Caco-2 epithelial cells and THP-1 macrophage cells against Streptococcus pyogenes and Escherichia coli]. Mikrobiyol Bul. 2009;43:373-381.
  • 23. Biriken D, Yazıhan N, Yılmaz Ş. [Investigation of cytokine and midkine responses of human THP-1 leukemia cells induced by phorbol-12-Myristate- 13-Acetate (PMA) at different concentrations and times]. Mikrobiyol Bul. 2018;52:147-155.

Differentiation of Human Leukemia Monocyte Cells to Macrophage-Like Cells with Phorbol- 12-Myristate-13-Acetate

Yıl 2019, Cilt: 72 Sayı: 1, 34 - 38, 13.06.2019

Derya Biriken

Öz

Objectives: In this study, it was aimed to evaluate appropriate model of application time and dosage of Phorbol-12-Myristate-13-Acetate (PMA) to get activated macrophages from monocytic cell line THP-1 cells. The differentiation of THP-1 cells into macrophages when stimulated with different dosage and application time of phorbol myristic acid (PMA) was shown morphologically and the effects on cell viability were evaluated.

Materials and Methods: THP-1 cells were produced as suspension as 2x105 cells/mL in 6-well plates. Afterwards, THP-1 monocyte cells were treated with PMA of 10, 20, 40, 60 ng/mL concentrations and incubated for 24, 48 and 72 hours. Differentiation of suspended THP-1 cells into adherent macrophage-like cells was confirmed by tissue-culture microscope by observing cell adherence to cell culture flasks and phenotypic changes. Cell viability was determined by trypan blue staining.

Results and Conclusion: The best results for phenotypic changes and cell viability for THP-1 cells were obtained with 20 ng/mL PMA at 48h incubation time and the cell viability was detected as 92.2%. The administration of 40 ng/mL PMA resulted in a significant decrease in cell count compared to 10 and 20 ng/ml PMA administration at the 48h, but no difference was observed between groups after 72h application. These findings show that the differentiation of THP-1 monocytes into macrophages needs to be optimized in order to reflect the real physiologic conditions of macrophage models used in in vitro studies. These findings suggest that THP-1 cells are well differentiated by 20 ng/mL PMA at 48h incubation.

Anahtar Kelimeler

Monocyte Macrophage Differentiation Phorbol-12-Myristate-13-Acetate

Etik Beyan

Hücre kültürü kullanıldığından etik kurul onayı alınmamıştır.

Destekleyen Kurum

-

Proje Numarası

-

Teşekkür

-

Kaynakça

  • 1. Janeway CA, Travers P, Walport M, et al. Immunobiology, The Immune System In Health and Disease. 5th Edition, Elsevier Science Ltd/Gardland; 2001.
  • 2. Gordon S. Mononuclear phagocytes in immune defence. In: Immunology, Seventh Edition, Ed.: Male D, Brostoff J, Roth DB. Roitt, USA: Mosby Elsevier 2006;181-202.
  • 3. Smythies LE, Sellers M, Clements RH, et al. Human intestinal macrophages display profound inflammatory anergy despite avid phagocytic and bacteriocidal activity. J Clin Invest. 2005;115:66-75.
  • 4. Delves PJ, Martin SJ, Burton DR, et al. Innate immunity, In: Roitt’s Essential Immunology. 11th ed. Blackwell Publishing, USA 2006;1-20.
  • 5. Si-Tahar M, Touqui L, Chignard M. Innate immunity and inflammation- -two facets of the same anti-infectious reaction. Clin Exp Immunol. 2009;156:194-198.
  • 6. Tsuchiya S, Yamabe M, Yamagochi Y, et al. Establishment and characterization of a human acute monocytic leukemia cell line (THP-1). Int J Cancer. 1980;26:171-176.
  • 7. Chanput W, Mes JJ, Wichers HJ. THP-1 cell line: an in vitro cell model for immune modulation approach. Int Immunopharmacol. 2014;23:37-45.
  • 8. Qin Z. The use of THP-1 cells as a model for mimicking the function and regulation of monocytes and macrophages in the vasculature. Atherosclerosis. 2012;221:2-11.
  • 9. Solberg R, Smith R, Almlof M, et al. Legumain expression, activity and secretion are increased during monocyte-to-macrophage differentiation and inhibited by atorvastatin. Biol Chem 2015;396: 71-80.
  • 10. Auwerx J. The human leukemia cell line, THP-1: a multifacetted model for the study of monocyte-macrophage differentiation. Experientia. 1991;47:22-31.
  • 11. Tominaga T, Suzuki M, Saeki H, et al. Establishment of an activated macrophage cell line, A-THP-1, and its properties. Tohoku J Exp Med. 1998;186:99-119.
  • 12. Schwende H, Fitzke E, Ambs P, et al. Differences in the state of differentiation of THP-1 cells induced by phorbol ester and 1,25-dihydroxyvitamin D3. J Leukoc Biol. 1996;59:555-561.
  • 13. Bodet C, Chandad F, Greiner D. Modulation of cytokine production by Porphyromonas gingivalis in a macrophage and epithelial cell co-culture model. Microbes Infect. 2005;7:448-456.
  • 14. Shiratori H, Feinweber C, Luckhardt S, et al. THP-1 and human peripheral blood mononuclear cell-derived macrophages differ in their capacity to polarize in vitro. Mol Immunol. 2017;88:58-68.
  • 15. Kohro T, Tanaka T, Murakami T, et al. A comparison of differences in the gene expression profiles of phorbol 12-myristate 13-acetate differentiated THP- 1 cells and human monocyte-derived macrophage. J Atheroscler Thromb. 2004;11:88-97.
  • 16. Zhao W, Sun Z, Wang, S, et al. Wnt1 Participates in Inflammation Induced by Lipopolysaccharide Through Upregulating Scavenger Receptor A and NFkB. Inflammation. 2015;38:1700-1706.
  • 17. Park EK, Jung HS, Yang HI, et al. Optimized THP-1 differentiation is required for the detection of responses to weak stimuli. Inflamm Res. 2007;56:45-50.
  • 18. Freshney RI. Culture of Animal Cells: A Manual of Basic Technique. 3rd edition; Wiley-Liss, New York, 2000.
  • 19. Hjort MR, Brenyo AJ, Finkelstein JN, et al. Alveolar epithelial cellmacrophage interactions affect oxygen-stimulated interleukin-8 release. Inflammation. 2003;27:137-145.
  • 20. Lund ME, To J, O’Brien BA, et al. The choice of phorbol 12-myristate 13-acetate differentiation protocol influences the response of THP- 1 macrophages to a pro-inflammatory stimulus. J Immunol Methods. 2016;430:64-70.
  • 21. Aldo PB, Craveiro V, Guller S, et al. Effect of culture conditions on the phenotype of THP-1 monocyte cell line. Am J Reprod Immunol. 2013;70:80-86.
  • 22. Salin DB, Albayrak N, Yıldız S, et al. [Investigation of bactericidal effect and nitric oxide responses of Caco-2 epithelial cells and THP-1 macrophage cells against Streptococcus pyogenes and Escherichia coli]. Mikrobiyol Bul. 2009;43:373-381.
  • 23. Biriken D, Yazıhan N, Yılmaz Ş. [Investigation of cytokine and midkine responses of human THP-1 leukemia cells induced by phorbol-12-Myristate- 13-Acetate (PMA) at different concentrations and times]. Mikrobiyol Bul. 2018;52:147-155.
Toplam 23 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Klinik Mikrobiyoloji
Bölüm Makaleler
Yazarlar

Derya Biriken 0000-0002-6254-4578

Proje Numarası -
Yayımlanma Tarihi 13 Haziran 2019
Yayımlandığı Sayı Yıl 2019 Cilt: 72 Sayı: 1

Kaynak Göster

APA Biriken, D. (2019). Differentiation of Human Leukemia Monocyte Cells to Macrophage-Like Cells with Phorbol- 12-Myristate-13-Acetate. Ankara Üniversitesi Tıp Fakültesi Mecmuası, 72(1), 34-38. https://doi.org/10.4274/atfm.galenos.2019.02886
AMA Biriken D. Differentiation of Human Leukemia Monocyte Cells to Macrophage-Like Cells with Phorbol- 12-Myristate-13-Acetate. Ankara Üniversitesi Tıp Fakültesi Mecmuası. Haziran 2019;72(1):34-38. doi:10.4274/atfm.galenos.2019.02886
Chicago Biriken, Derya. “Differentiation of Human Leukemia Monocyte Cells to Macrophage-Like Cells With Phorbol- 12-Myristate-13-Acetate”. Ankara Üniversitesi Tıp Fakültesi Mecmuası 72, sy. 1 (Haziran 2019): 34-38. https://doi.org/10.4274/atfm.galenos.2019.02886.
EndNote Biriken D (01 Haziran 2019) Differentiation of Human Leukemia Monocyte Cells to Macrophage-Like Cells with Phorbol- 12-Myristate-13-Acetate. Ankara Üniversitesi Tıp Fakültesi Mecmuası 72 1 34–38.
IEEE D. Biriken, “Differentiation of Human Leukemia Monocyte Cells to Macrophage-Like Cells with Phorbol- 12-Myristate-13-Acetate”, Ankara Üniversitesi Tıp Fakültesi Mecmuası, c. 72, sy. 1, ss. 34–38, 2019, doi: 10.4274/atfm.galenos.2019.02886.
ISNAD Biriken, Derya. “Differentiation of Human Leukemia Monocyte Cells to Macrophage-Like Cells With Phorbol- 12-Myristate-13-Acetate”. Ankara Üniversitesi Tıp Fakültesi Mecmuası 72/1 (Haziran 2019), 34-38. https://doi.org/10.4274/atfm.galenos.2019.02886.
JAMA Biriken D. Differentiation of Human Leukemia Monocyte Cells to Macrophage-Like Cells with Phorbol- 12-Myristate-13-Acetate. Ankara Üniversitesi Tıp Fakültesi Mecmuası. 2019;72:34–38.
MLA Biriken, Derya. “Differentiation of Human Leukemia Monocyte Cells to Macrophage-Like Cells With Phorbol- 12-Myristate-13-Acetate”. Ankara Üniversitesi Tıp Fakültesi Mecmuası, c. 72, sy. 1, 2019, ss. 34-38, doi:10.4274/atfm.galenos.2019.02886.
Vancouver Biriken D. Differentiation of Human Leukemia Monocyte Cells to Macrophage-Like Cells with Phorbol- 12-Myristate-13-Acetate. Ankara Üniversitesi Tıp Fakültesi Mecmuası. 2019;72(1):34-8.
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