Öz
Compared with conventional light microscopy (LLM), confocal mic- roscopy (CM) is characterized in part by increased light sensitivity and higher spatial resolution particulariy in using the laser beam as a light source, These characteristics permit the detection and diserimination of minor structures in cells and tissues in three dimensions with a more specific, sensitive and accurate way. One of the most important feature of this microscope is to avoid the effects of out-of-focus
images in the final image by means of object plane sectioning and pinhole diaphragm. Potential clinical applications related to these characteristics of confocal microscopy are discussed and concluded that its olinical impact is obvious.
Anahtar Kelimeler
Conventional light microscope confocal microscope cell and tissue
Kaynakça
- 1. Baak JPA ve ark : Potential cliniçal uses of laser scan microscopy. Appl Optics 28 : 3414, 1987.
- 2. Bultmann B ve ark ; Lateral diffussion of ehemotactic peptide receptors within the cytoplasmic membrane of human PMNs demonstrated by Laser Scan MicToscopy. Adv Biosci 68 : 47, 1987,
- 3. Cogswell CI Sheppard CIR ; Imaging using confocal brightfisld tecnigues. JInst Phys Conf Ser No : 98, 633, 1989,
- 4.Dixon AJ : Principles and applications of confocal fluorescence miCTosCOpy. InstPhys Conf Ser No : 88, 643, 1989.
- 5.Elder HY, Goodhew PJ : Three dimensional microscopy and confocal microscopy. m EMAG-MICRO 89., 1989, Eastern Press, sayfa : 609-642.
- 6.Epstein JI Berry 5J Egeleston JG : Nuclear roundness İactor : A predictor of progression in untreated stage A2 prostate cancer, Cancer 54 : 1666, 1984.
- 7.Jorgens R Godecke U ; The second generation L5M more than merely cosmetic correction. Mikro express : 3İ, 1989.
- 8.Koch GLE, Macer DRJ Smith MJ : Visualisation of the intact endoplasmic reticulum by immunoflluorescence with antibodies to the major ER giycoprotein, endoplasmin. J Cell Sci 87 : 585, 1987.
- 9.Monro S Pelham HRB:A C-terminal signal, prevents secretion of luminal ER protsins, Cell 48 : 899, 1987. Niçoud M-R ve ark : 3-D Imaging of celiş and Tissues Usin Confoca ILaser 10.Scanning Microscopy and Digital Processing. Fur J Cell Biol 47 : 234, 1988.
- 11.Pawley JB : The micro world has three dimensions and sometimes four, Inst phys Conf Ser No : 88, 609, 1989.
- 12.Sassen RCI Baak JPA : Morphometry in the differential diagnosis of granulosa-cell tumors of the ovary. Anal Guant Cytol Histol 8 : 245, 1986.
- 13.Shotton DM : The current renajssance in ight microscopy. L Dynamic studies of living cellis by video enhanced contrast microscopy, Proc Royal Microscopical Soc 22 : 37, 1987.
- 14.Shotton D : The current Renaissance in Light Microscopy. LI. Blur-Free Optical Sectioning of Biological Specimens by Confocal Scanning Fluorescence Microscopy. Proc Royal Microscopical Soç 25 : 289, 1988,
- 15.Dixon AJ : Principles and applications of confocal fluorescence miCTosCOpy. Inst Phys Conf Ser No : 88, 643, 1989.
- 16.Van der Linden HC Baak JPA Lindeman J Hermans J Meijer CILM : Morphometry and breast cancer 11. Characterisation ol breast cancer cells with high malignant potential in patients with spread to Iymph nodes : Preliminary Results. J Clin Pathol 39 ; 603, 1986. i
- 17.White JG Amos WB Fordham M : An evaluation of confocal versus conventional imaging of biological structures by fluorescence light microscopy. J Cell Biol 105 : 41, 1987.
Öz
Konvansiyonel ışık mikroskobu ile kıyaslandığında konfokal mikroskobu, özellikle ışık kaynağı olarak laserin kullanıldığı durumlarda, ışık duyarlığında ve uzaysal düzlemdeki çözüm gücündeki artış ile karakterizedir. Bu özellikler, hücre ve dokulardaki ince yapıyı daha spe- sifik, hassas ve kesin şekliyle üç boyutlu olarak ortaya çıkarma ve degerlendirme olanağı tanır, Bu mikroskobun en önemli özelliklerinden birisi de fokus dışı görüntülerin, düzlem kesit alma özelliği ve iğne deliği diyafram yardımıyla final görüntüyü etkilemesini önlemesidir, Bu özellikleri ile bu mikroskobun klinik alanlardaki kullanım potansiyeli tartışılmış ve etkisinin son derece yararlı olduğu sonucuna varılmıştır.
Anahtar Kelimeler
Konvansiyonel ışık mikroskobu konfokal mikroskobu hücre ve dokul
Kaynakça
- 1. Baak JPA ve ark : Potential cliniçal uses of laser scan microscopy. Appl Optics 28 : 3414, 1987.
- 2. Bultmann B ve ark ; Lateral diffussion of ehemotactic peptide receptors within the cytoplasmic membrane of human PMNs demonstrated by Laser Scan MicToscopy. Adv Biosci 68 : 47, 1987,
- 3. Cogswell CI Sheppard CIR ; Imaging using confocal brightfisld tecnigues. JInst Phys Conf Ser No : 98, 633, 1989,
- 4.Dixon AJ : Principles and applications of confocal fluorescence miCTosCOpy. InstPhys Conf Ser No : 88, 643, 1989.
- 5.Elder HY, Goodhew PJ : Three dimensional microscopy and confocal microscopy. m EMAG-MICRO 89., 1989, Eastern Press, sayfa : 609-642.
- 6.Epstein JI Berry 5J Egeleston JG : Nuclear roundness İactor : A predictor of progression in untreated stage A2 prostate cancer, Cancer 54 : 1666, 1984.
- 7.Jorgens R Godecke U ; The second generation L5M more than merely cosmetic correction. Mikro express : 3İ, 1989.
- 8.Koch GLE, Macer DRJ Smith MJ : Visualisation of the intact endoplasmic reticulum by immunoflluorescence with antibodies to the major ER giycoprotein, endoplasmin. J Cell Sci 87 : 585, 1987.
- 9.Monro S Pelham HRB:A C-terminal signal, prevents secretion of luminal ER protsins, Cell 48 : 899, 1987. Niçoud M-R ve ark : 3-D Imaging of celiş and Tissues Usin Confoca ILaser 10.Scanning Microscopy and Digital Processing. Fur J Cell Biol 47 : 234, 1988.
- 11.Pawley JB : The micro world has three dimensions and sometimes four, Inst phys Conf Ser No : 88, 609, 1989.
- 12.Sassen RCI Baak JPA : Morphometry in the differential diagnosis of granulosa-cell tumors of the ovary. Anal Guant Cytol Histol 8 : 245, 1986.
- 13.Shotton DM : The current renajssance in ight microscopy. L Dynamic studies of living cellis by video enhanced contrast microscopy, Proc Royal Microscopical Soc 22 : 37, 1987.
- 14.Shotton D : The current Renaissance in Light Microscopy. LI. Blur-Free Optical Sectioning of Biological Specimens by Confocal Scanning Fluorescence Microscopy. Proc Royal Microscopical Soç 25 : 289, 1988,
- 15.Dixon AJ : Principles and applications of confocal fluorescence miCTosCOpy. Inst Phys Conf Ser No : 88, 643, 1989.
- 16.Van der Linden HC Baak JPA Lindeman J Hermans J Meijer CILM : Morphometry and breast cancer 11. Characterisation ol breast cancer cells with high malignant potential in patients with spread to Iymph nodes : Preliminary Results. J Clin Pathol 39 ; 603, 1986. i
- 17.White JG Amos WB Fordham M : An evaluation of confocal versus conventional imaging of biological structures by fluorescence light microscopy. J Cell Biol 105 : 41, 1987.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Histoloji ve Embriyoloji |
| Bölüm | Makaleler |
| Yazarlar | |
| Yayımlanma Tarihi | 30 Eylül 1990 |
| Yayımlandığı Sayı | Yıl 1990 Cilt: 43 Sayı: 3 |
