Öz
Blood cultures were obtained from healthy newborns at first week of life, newborns with hyperbilirubinemia before and after the phototherapy for the analysis of cell kinetics with a repeat two days later. Cultures were carried on dark with a final concentration of BrdU 10 mg/ml. Slides were screened for the first, second and the third
mitotic division. Cell Kinetics Index (CKI) was evaluated with the formula of CKI — (M1+2M2+3.M3)/100. Findings were suggested no obvious effect has phototherapy on CKI
Anahtar Kelimeler
Etik Beyan
-
Destekleyen Kurum
-
Proje Numarası
-
Teşekkür
-
Kaynakça
- 1. Das BC Sharma T : The fate of X-ray-induced chromosome aberrations in blood lymphocyte culture, Mutat. Res., 176 : 83-104, 1987.
- 2. Balakrishna Murthy P Abdul Rahiman M Tulpule PG :UV-Induced Chromosome Aberrations, Sister-Chromatid Exchanges and Cell Survival in Cultured Lymphocytes from Malnourished Children. Pediatr. Res., 16 : 663-664, 1982.
- 3. Vogel F Motulsky AG : Human Genetics, Problems and Approaches. Second ed. : Springer-Verlag Berlin Heidelberg New York, pp 330-359, 1982.
- 4. Kilbey BJ Legator M Nichols W Ramel C : Handbook of Mutagenicity Test Procedures : Elseiver Scintific Publishing Company, 1877.
- 5. Tucker JD Christensen ML : Effects of antikoagulants upon sister-chromatid exchanges, cell-cycle kinetics, and mitotic index in human peripheral lymphocytes. Mutat. Res., 180 : 225-228, 1987.
- 6. Mutchinick O Ruz L Gonsebatt ME Mauleon P Lisker R Garcia G : Frequency and Types of Induced Spontaneous Chromosome Aberrations in Relation to Cell Kinetics, Hum. Genet., 59 : 137-140.
- 7. Korenberg IR Freedlender EF : Giemsa tecnique for the detection of SCE. Chromosoma, 48 : 355-360, 1874.
- 8.Demirsoy D Tungbilek E Oran O : Fototerapinin kromozomlar uzerindeki etkisinin in vivo <Sister Chromatid Exchange- teknigi ile araştırılması. Çovuk Saghığı ve Hastalıkları Dergisi, 30 : 17-27, 1987,
- 9.Sideris EG Papageorgiou GC Charalampous SC Vitsa EM : A Spectrum Response Study on Single Strand DNA Breaks Sister Chromatid Exchanges. and Lethality Induced by Phototherapy Lights. Pediatr. Res., 15 : 1019-1023, 1981
- 10.Hatcher NH Risemberg HM Powers MM Hook EB : Sister Chromatid Exchange and Phototherapy. Mutat. Res., 60 : 401, 1970.
Öz
Sağlıklı yenidoğanlardan doğumu izleyen ilk bir hafta içinde ve hiperbilirübinemik yenidoğanlardan fototerapi öncesi, sonrası ve iki gün sonrası alınan periferik kan, 10 mg/ml final konsantrasyonda BrdU içeren vasatlara ekilmiştir. Karanlıkta yürütülen kültür işlemi sonunda hazırlanan preparatlarda birinci, ikinci ve üçüncü mitozlar
sayılmıştır. Hücre kinetik indeksi (CKI) CKI = (M1 +2.M2+3.M3/100 formülü kullamlarak hesaplanmistir, Elde edilen sonuçlar fototerapinin CKI tzerinde ¢ok belirgin bir etkisinin olmadığını düşündürmüştür.
Etik Beyan
-
Destekleyen Kurum
-
Proje Numarası
-
Teşekkür
-
Kaynakça
- 1. Das BC Sharma T : The fate of X-ray-induced chromosome aberrations in blood lymphocyte culture, Mutat. Res., 176 : 83-104, 1987.
- 2. Balakrishna Murthy P Abdul Rahiman M Tulpule PG :UV-Induced Chromosome Aberrations, Sister-Chromatid Exchanges and Cell Survival in Cultured Lymphocytes from Malnourished Children. Pediatr. Res., 16 : 663-664, 1982.
- 3. Vogel F Motulsky AG : Human Genetics, Problems and Approaches. Second ed. : Springer-Verlag Berlin Heidelberg New York, pp 330-359, 1982.
- 4. Kilbey BJ Legator M Nichols W Ramel C : Handbook of Mutagenicity Test Procedures : Elseiver Scintific Publishing Company, 1877.
- 5. Tucker JD Christensen ML : Effects of antikoagulants upon sister-chromatid exchanges, cell-cycle kinetics, and mitotic index in human peripheral lymphocytes. Mutat. Res., 180 : 225-228, 1987.
- 6. Mutchinick O Ruz L Gonsebatt ME Mauleon P Lisker R Garcia G : Frequency and Types of Induced Spontaneous Chromosome Aberrations in Relation to Cell Kinetics, Hum. Genet., 59 : 137-140.
- 7. Korenberg IR Freedlender EF : Giemsa tecnique for the detection of SCE. Chromosoma, 48 : 355-360, 1874.
- 8.Demirsoy D Tungbilek E Oran O : Fototerapinin kromozomlar uzerindeki etkisinin in vivo <Sister Chromatid Exchange- teknigi ile araştırılması. Çovuk Saghığı ve Hastalıkları Dergisi, 30 : 17-27, 1987,
- 9.Sideris EG Papageorgiou GC Charalampous SC Vitsa EM : A Spectrum Response Study on Single Strand DNA Breaks Sister Chromatid Exchanges. and Lethality Induced by Phototherapy Lights. Pediatr. Res., 15 : 1019-1023, 1981
- 10.Hatcher NH Risemberg HM Powers MM Hook EB : Sister Chromatid Exchange and Phototherapy. Mutat. Res., 60 : 401, 1970.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Tıbbi Mikrobiyoloji (Diğer) |
| Bölüm | Makaleler |
| Yazarlar | |
| Proje Numarası | - |
| Yayımlanma Tarihi | 30 Eylül 1990 |
| Yayımlandığı Sayı | Yıl 1990 Cilt: 43 Sayı: 3 |
