Araştırma Makalesi
Efficacy of a modified albendazole and ivermectin formulation on treatment and the seasonal dynamics of sheep helminthosis
Öz
The aim of this study was to develop a complex antiparasitic compound containing albendazole (ABZ) and ivermectin (IVM) based on solid dispersion to increase the bioavailability of the active substances, to evaluate the efficacy of the formulation, and to monitor the effect of its use on the seasonal dynamics of sheep helminthosis for one year. To obtain the complex, a mechanochemical technology with joint grinding of ABZ, IVM, and polymer (polyvinylpyrrolidone, PVP) was used. The formulation was then used to study its efficacy in sheep helminthosis using coprological methods. Additionally, the sheep were treated both in spring and autumn to study the seasonal dynamics of infection with nematodes, cestodes, and trematodes. The suspension, administered at a rate of 0.2 mg IVM and 3 mg ABZ/kg body weight, showed 100% efficacy against the entire complex of helminths in both spring and autumn treatments. Furthermore, sheep admitted to winter stall maintenance presented fairly low rates of extensiveness (10.0–13.7%) and intensity (9.5–14.7 EPG) of infection with all types of helminths. The present study showed that administrations of the newly formulated anthelminthic (CAPS-AI) twice in the year, i.e., in the spring (before going out to pasture) and in the fall (before placing them in stalls), ensured a relatively low level of sheep helminthoses, making it possible to prevent the clinical manifestation of helminthosis and subsequently economic losses.
Anahtar Kelimeler
Anthelminthic Mechanochemical modification Novel formulation Parasiticidal activity Solid dispersion
Kaynakça
- Arkhipov IA, Varlamova AI, Radionov AV (2022): Evaluation of the efficacy of benzimidazole anthelmintics against different stages of gastrointestinal nematodes of young cattle. Rus J Parasitol, 16, 335-340.
- Arkhipov IA, Sadov KM, Limova YV, et al (2017): The efficacy of the supramolecular complexes of niclosamide obtained by mechanochemical technology and targeted delivery against cestode infection of animals. Vet Parasitol, 246, 25-29.
- Ashrafzadeh-Shiraz M, Tavassoli M, Dalir-Naghadeh B, et al (2024): Impaired efficacy of fenbendazole and ivermectin against intestinal nematodes in adult horses in Iran. Res Vet Sci, 166, 105078.
- Baihaqi ZA, Widiyono I, Nurcahyo W (2019): Prevalence of gastrointestinal worms in Wonosobo and thin-tailed sheep on the slope of Mount Sumbing, Central Java, Indonesia. Vet World, 12, 1866-1871.
- Campbell WC, Benz GW (1984): Ivermectin: a review of efficacy and safety. J Vet Pharmacol Therapy, 7, 1-16.
- Campbell WC (2009): Benzimidazoles: Veterinary uses. Parasitol Today, 6, 130-133.
- Efremova EA, Marchenko VA (2014): Features of the structure of the helminth complex and the dynamics of infection of sheep in the Altai Republic. Siberian Bull Agric Sci, 6, 82-88.
- Efremova EA, Marchenko VA (2017): Helminths of the suborder Strongylata of sheep and features of their territorial distribution in the Altai Republic. Vet Doctor, 4, 53-59.
- Engasheva ES, Moskalev VG, Muromtsev AB (2019): Efficiency of the drug Monizen forte against helminthosis and arachnoenthomosis of sheep. 205-209. In: Theory and Practice of Combating Parasitic Diseases, Vol. 20, Moscow.
- Greer W, Wyk JA, Hamie JC, et al (2020): Refugia-based strategies for parasite control in livestock. Vet Clin N Am Food Anim Pract, 36, 31-43.
- Idris A, Moors E, Sohnrey B, et al (2012): Gastrointestinal nematode infections in German sheep. Parasitol Res, 110, 1453-1459.
- Khalikov SS, Lokshin BV, Ilyin MM, et al (2019): Methods for obtaining solid dispersions of drugs and their properties. Rus Chem Bull, 68, 1924-1932.
- Khalikov SS, Lokshin BV, Ilyin MM (2020): Solid dispersions of medamine and albendazole with water-soluble polymers: preparation and properties. Pharm Chem J, 54, 816–821.
- Khalikov SS, Khakina EA, Khalikov MS, et al (2023): Solid dispersions of fenbendazole with polymers and succinic acid obtained via methods of mechanochemistry: their chemical stability and anthelmintic efficiency. Powders, 2, 727–736.
- Kipp K, Cummings DB, Goehl D, et al (2023): Evaluation of a refugia-based strategy for gastrointestinal nematodes on weight gain and fecal egg counts in naturally infected stocker calves administered combination anthelmintics. Vet Parasitol, 319, 109955.
- Kotelnikov GA (1984): Helminthological studies of animals and the environment. Kolos, Moscow.
- Kurinov DA (2021): Therapeutic efficiency of alben for intestinal strongylatosis of red deer of the Central Altai. 158-161. In: Actual Problems of Agriculture in Mountain Areas. Gorno-Altai State University.
- Marchenko VA, Efremova EA, Vasilyeva EA (2008): Structure of helminthocenosis of cattle in the Altai Mountains. Rus J Parasitol, 3, 18-23.
- Marchenko VA, Vasilenko YA (2015): The structure of the helminth complex of sheep in the Altai Mountains and the effectiveness of a dry concentrate of an antiparasitic suspension for helminthiasis of sheep. Rus J Parasitol, 9, 7-14.
- Marchenko VA, Vasilenko YA, Efremova EA (2016): The influence of environmental factors on the infection of sheep with intestinal helminths in the Altai Mountains. Bull Novosibirsk State Agrarian Univ, 3, 129-137.
- Marchenko VA, Vasilenko YA, Efremova EA (2017): Efficiency of complex parasiticidal agents in sheep farming in the Altai Mountains. Bull Altai State Agrarian Univ, 10, 105-113.
- Marchenko VA, Efremova EA (2019): Epizootic situation regarding helminthosis of farm animals in the Altai Republic. 341-346. In: Theory and Practice of Combating Parasitic Diseases Vol. 20, Moscow.
- Marchenko VA, Khalikov SS, Efremova EA, et al (2019): Efficiency of solid dispersions of ivermectin and albendazole against intestinal helminthiasis of sheep in the Altai Republic. Bull Novosibirsk State Agrarian Univ, 3, 82-90.
- Marchenko VA, Khalikov SS, Vasilenko YA, et al (2020): Innovative anthelmintic based on mechanochemical technology and their efficacy against parasitic infection of sheeps. J Adv Vet Animal Res, 7, 718-725.
- Marchenko VA, Khalikov SS, Efremova EA, et al (2021): Efficacy of novel formulations of ivermectin and albendazole in parasitic infections of sheep in the Altai Mountains of Russia. Iran J Parasitol, 16, 198-208.
- Marchenko VA, Khalikov SS, Biryukov IV, et al (2023): Synthesis and clinical examination of novel formulations of ivermectin, albendazole and niclosamide for the treatment of equine gastrointestinal helminthoses. Iran J Parasitol, 18, 66-75.
- Mphahlele M, Tsotetsi-Khambule AM, Moerane R, et al (2021): Anthelmintic resistance and prevalence of gastrointestinal nematodes infecting sheep in Limpopo Province, South Africa, Vet World, 14, 302-313.
- Musaev MB, Zashchepkina VV, Khalikov SS (2020): Antiparasitic complex of ivermectin for the treatment of herd horses with nematodes of the digestive tract. Rus J Parasitol, 14, 114-119.
- Qamar W, Alkheraije KA (2023): Anthelmintic resistance in Haemonchus contortus of sheep and goats from Asia–a review of in vitro and in vivo studies. Pak Vet J, 43, 376-387.
- Singh E, Kaur P, Singla LD, et al (2017): Prevalence of gastrointestinal parasitism in small ruminants in western zone of Punjab, India. Vet World, 10, 61-66.
- Takagi T, Ramachandran Ch, Bermejo M, et al (2006): A provisional biopharmaceutical classification of the top 200 oral drug products in the United States, Great Britain, Spain, and Japan. Mol Pharm, 3, 631-646.
- Tsepilova II, Shemyakova SA, Nikolaeva EA (2022): Helminth fauna of sheep in the valley of the Teberda River. 484-489. In: Theory and Practice of Combating Parasitic Diseases Vol. 23, Moscow
- Vemula VR, Lagishetty V, Lingala S (2010): Solubility enhancement techniques. Int J Pharm Sci Rev Res, 5, 41-51.
- Win SY, Win M, Thwin EP, et al (2020): Occurrence of gastrointestinal parasites in small ruminants in the central part of Myanmar. J Parasitol Res, 25, 8826327.
Yıl 2025,
Accepted Papers, 1 - 11
Öz
Kaynakça
- Arkhipov IA, Varlamova AI, Radionov AV (2022): Evaluation of the efficacy of benzimidazole anthelmintics against different stages of gastrointestinal nematodes of young cattle. Rus J Parasitol, 16, 335-340.
- Arkhipov IA, Sadov KM, Limova YV, et al (2017): The efficacy of the supramolecular complexes of niclosamide obtained by mechanochemical technology and targeted delivery against cestode infection of animals. Vet Parasitol, 246, 25-29.
- Ashrafzadeh-Shiraz M, Tavassoli M, Dalir-Naghadeh B, et al (2024): Impaired efficacy of fenbendazole and ivermectin against intestinal nematodes in adult horses in Iran. Res Vet Sci, 166, 105078.
- Baihaqi ZA, Widiyono I, Nurcahyo W (2019): Prevalence of gastrointestinal worms in Wonosobo and thin-tailed sheep on the slope of Mount Sumbing, Central Java, Indonesia. Vet World, 12, 1866-1871.
- Campbell WC, Benz GW (1984): Ivermectin: a review of efficacy and safety. J Vet Pharmacol Therapy, 7, 1-16.
- Campbell WC (2009): Benzimidazoles: Veterinary uses. Parasitol Today, 6, 130-133.
- Efremova EA, Marchenko VA (2014): Features of the structure of the helminth complex and the dynamics of infection of sheep in the Altai Republic. Siberian Bull Agric Sci, 6, 82-88.
- Efremova EA, Marchenko VA (2017): Helminths of the suborder Strongylata of sheep and features of their territorial distribution in the Altai Republic. Vet Doctor, 4, 53-59.
- Engasheva ES, Moskalev VG, Muromtsev AB (2019): Efficiency of the drug Monizen forte against helminthosis and arachnoenthomosis of sheep. 205-209. In: Theory and Practice of Combating Parasitic Diseases, Vol. 20, Moscow.
- Greer W, Wyk JA, Hamie JC, et al (2020): Refugia-based strategies for parasite control in livestock. Vet Clin N Am Food Anim Pract, 36, 31-43.
- Idris A, Moors E, Sohnrey B, et al (2012): Gastrointestinal nematode infections in German sheep. Parasitol Res, 110, 1453-1459.
- Khalikov SS, Lokshin BV, Ilyin MM, et al (2019): Methods for obtaining solid dispersions of drugs and their properties. Rus Chem Bull, 68, 1924-1932.
- Khalikov SS, Lokshin BV, Ilyin MM (2020): Solid dispersions of medamine and albendazole with water-soluble polymers: preparation and properties. Pharm Chem J, 54, 816–821.
- Khalikov SS, Khakina EA, Khalikov MS, et al (2023): Solid dispersions of fenbendazole with polymers and succinic acid obtained via methods of mechanochemistry: their chemical stability and anthelmintic efficiency. Powders, 2, 727–736.
- Kipp K, Cummings DB, Goehl D, et al (2023): Evaluation of a refugia-based strategy for gastrointestinal nematodes on weight gain and fecal egg counts in naturally infected stocker calves administered combination anthelmintics. Vet Parasitol, 319, 109955.
- Kotelnikov GA (1984): Helminthological studies of animals and the environment. Kolos, Moscow.
- Kurinov DA (2021): Therapeutic efficiency of alben for intestinal strongylatosis of red deer of the Central Altai. 158-161. In: Actual Problems of Agriculture in Mountain Areas. Gorno-Altai State University.
- Marchenko VA, Efremova EA, Vasilyeva EA (2008): Structure of helminthocenosis of cattle in the Altai Mountains. Rus J Parasitol, 3, 18-23.
- Marchenko VA, Vasilenko YA (2015): The structure of the helminth complex of sheep in the Altai Mountains and the effectiveness of a dry concentrate of an antiparasitic suspension for helminthiasis of sheep. Rus J Parasitol, 9, 7-14.
- Marchenko VA, Vasilenko YA, Efremova EA (2016): The influence of environmental factors on the infection of sheep with intestinal helminths in the Altai Mountains. Bull Novosibirsk State Agrarian Univ, 3, 129-137.
- Marchenko VA, Vasilenko YA, Efremova EA (2017): Efficiency of complex parasiticidal agents in sheep farming in the Altai Mountains. Bull Altai State Agrarian Univ, 10, 105-113.
- Marchenko VA, Efremova EA (2019): Epizootic situation regarding helminthosis of farm animals in the Altai Republic. 341-346. In: Theory and Practice of Combating Parasitic Diseases Vol. 20, Moscow.
- Marchenko VA, Khalikov SS, Efremova EA, et al (2019): Efficiency of solid dispersions of ivermectin and albendazole against intestinal helminthiasis of sheep in the Altai Republic. Bull Novosibirsk State Agrarian Univ, 3, 82-90.
- Marchenko VA, Khalikov SS, Vasilenko YA, et al (2020): Innovative anthelmintic based on mechanochemical technology and their efficacy against parasitic infection of sheeps. J Adv Vet Animal Res, 7, 718-725.
- Marchenko VA, Khalikov SS, Efremova EA, et al (2021): Efficacy of novel formulations of ivermectin and albendazole in parasitic infections of sheep in the Altai Mountains of Russia. Iran J Parasitol, 16, 198-208.
- Marchenko VA, Khalikov SS, Biryukov IV, et al (2023): Synthesis and clinical examination of novel formulations of ivermectin, albendazole and niclosamide for the treatment of equine gastrointestinal helminthoses. Iran J Parasitol, 18, 66-75.
- Mphahlele M, Tsotetsi-Khambule AM, Moerane R, et al (2021): Anthelmintic resistance and prevalence of gastrointestinal nematodes infecting sheep in Limpopo Province, South Africa, Vet World, 14, 302-313.
- Musaev MB, Zashchepkina VV, Khalikov SS (2020): Antiparasitic complex of ivermectin for the treatment of herd horses with nematodes of the digestive tract. Rus J Parasitol, 14, 114-119.
- Qamar W, Alkheraije KA (2023): Anthelmintic resistance in Haemonchus contortus of sheep and goats from Asia–a review of in vitro and in vivo studies. Pak Vet J, 43, 376-387.
- Singh E, Kaur P, Singla LD, et al (2017): Prevalence of gastrointestinal parasitism in small ruminants in western zone of Punjab, India. Vet World, 10, 61-66.
- Takagi T, Ramachandran Ch, Bermejo M, et al (2006): A provisional biopharmaceutical classification of the top 200 oral drug products in the United States, Great Britain, Spain, and Japan. Mol Pharm, 3, 631-646.
- Tsepilova II, Shemyakova SA, Nikolaeva EA (2022): Helminth fauna of sheep in the valley of the Teberda River. 484-489. In: Theory and Practice of Combating Parasitic Diseases Vol. 23, Moscow
- Vemula VR, Lagishetty V, Lingala S (2010): Solubility enhancement techniques. Int J Pharm Sci Rev Res, 5, 41-51.
- Win SY, Win M, Thwin EP, et al (2020): Occurrence of gastrointestinal parasites in small ruminants in the central part of Myanmar. J Parasitol Res, 25, 8826327.
Toplam 34 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Veteriner Hekimlik |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Erken Görünüm Tarihi | 14 Temmuz 2025 |
| Yayımlanma Tarihi | |
| Gönderilme Tarihi | 2 Eylül 2024 |
| Kabul Tarihi | 13 Haziran 2025 |
| Yayımlandığı Sayı | Yıl 2025 Accepted Papers |