Comparative study of immunocytological, immunohistochemical and in-situ hybridization methods in small ruminant neonatal mortality

Sevil Atalay Vural; Rıfkı Hazıroğlu; Osman Kutsal; Gözde Yücel Tenekeci; Arda Selin Tunç; Yanad Abou Monsef; Ozan Ahlat; Kürşat Filikci; Özgür Özöner; Oya Burçin Demirtaş

doi:10.33988/auvfd.1526014

Araştırma Makalesi

BibTex

RIS

Kaynak Göster

Comparative study of immunocytological, immunohistochemical and in-situ hybridization methods in small ruminant neonatal mortality

Yıl 2025, Cilt: 72 Sayı: 3, 277 - 286, 01.07.2025

Sevil Atalay Vural , Rıfkı Hazıroğlu , Osman Kutsal , Gözde Yücel Tenekeci , Arda Selin Tunç , Yanad Abou Monsef , Ozan Ahlat , Kürşat Filikci , Özgür Özöner , Oya Burçin Demirtaş

https://doi.org/10.33988/auvfd.1526014

Öz

It was aimed to identify diseases using immunocytochemical, immunohistochemical, and in-situ hybridization methods, to determine sensitivity and specificity among these techniques, and to highlight their advantages and disadvantages for certain respiratory (Mycoplasma pneumonia, Pasteurella spp., Respiratory syncytial virus, Parainfluenza virus 3) and enteric (Coronavirus, Rotavirus, Escherichia coli, Clostridium spp.) agents. The obtained results were compared, and although the immunocytochemical method was found to be the fastest, immunohistochemistry was proved to be the most reliable method. Our other aim was to establish pathological diagnostic panels for neonatal infections. All antibodies tested were found to be positive except for Pasteurella multocida. The immunohistochemical findings of the study indicate that nearly all cases that result in death involved mixed infections.

Anahtar Kelimeler

Immunocytochemistry, Immunohistochemistry, infection, in situ hybridisation, neonatal small ruminant

Etik Beyan

This study does not present any ethical concerns.

Proje Numarası

21A0239003

Kaynakça

1. Baghezza S, Mamache B, Bennoune O, et al (2021): Pathological study and detection of Bovine parainfluenza 3 virus in pneumonic sheep lungs using direct immunofluorescence antibody technique. Comp Clin Path, 30, 301–310.
2. Benavides J, González L, Dagleish M, et al (2015): Diagnostic pathology in microbial diseases of sheep or goats. Vet Microbiol, 181, 15-26.
3. Caswell J L, Williams KJ (2015): Respiratory system. In: Jubb, Kennedy, and Palmer’s pathology of domestic animals, Volume 2, 6th edn., Maxie MG (ed), Elsevier, St. Louis, Missouri. pp 465–591.
4. Chakraborty S, Kumar A, Tiwari R, et al (2014): Advances in diagnosis of respiratory diseases of small ruminants. Vet Med Int, 2014, 508304.
5. Chan S, Filézac de L’Etang A, Rangell L, et al (2018): A method for manual and automated multiplex RNAscope in situ hybridization and immunocytochemistry on cytospin samples. Plos One, 13, e0207619.
6. Çeribaşı AO, Ozkaraca M, Çeribaşı S, et al (2014): Histopathologic, immunoperoxidase and immunofluorescent examinations on natural cattle pneumonia originated from Parainfluenza type 3, Respiratory Syncytial virus, Adenovirus type 3 and Herpesvirus type 1. Rev Med Vet, 165, 201-212.
7. Durham PJ, Stevenson BJ, Farquharson BC (1979): Rotavirus and coronavirus associated diarrhoea in domestic animals. N Z Vet J, 27, 30–32.
8. Heller MC, Chigerwe M (2018): Diagnosis and treatment of infectious enteritis in neonatal and juvenile ruminants. Veterinary Clinics: Food Animal Practice, 34, 101-117.
9. Hindson JC, Winter A (2008): Manual of sheep diseases, 2nd edition. 90-102. John Wiley & Sons.
10. Holmøy IH, Waage S, Granquist EG, et al (2017): Early neonatal lamb mortality: postmortem findings. Animal, 11, 295-305.
11. Jarikre TA, Emikpe BO (2017): First report of immunohistochemical detection of Peste des petit ruminants, parainfluenza 3 and respiratory syncytial viral antigens in lungs of Nigerian goats. J Immunoassay Immunochem, 38, 555–568.
12. Martella V, Decaro N, Buonavoglia C (2015): Enteric viral infections in lambs or kids. Vet Microbiol, 181, 154-160.
13. Munday JS, Bentall H, Aberdein D, et al (2020): Death of a neonatal lamb due to Clostridium perfringens type B in New Zealand. N Z Vet J, 68, 242–246.
14. Ozmen O, Yukari BA, Haligur M, et al (2006): Observations and immunohistochemical detection of Coronavirus, Cryptosporidium parvum and Giardia intestinalis in neonatal diarrhoea in lambs and kids. Schweiz Arch Tierh, 148, 357–364.
15. Ozmen O, Haligur M, Aydogan A, et al (2018): Immunohistochemical detection of viral etiopathogenesis in lambs and goat kids with neonatal diarrhea. Acta Sci Vet, 46, 8.
16. Sheehan M, Cassidy JP, Brady J, et al (2007): An aetiopathological study of chronic bronchopneumonia in lambs in Ireland. Veterinary journal (London, England, 1997), 173, 630–637.
17. Terzi OS, Kara E, Şenel Y, et al (2022): Dynamic thiol-disulphide homeostasis and ischemia modified albumin levels in neonatal calf diarrhea. Ank Univ Vet Fak Derg, 70, 81-86.
18. Uzal FA, Plattner BL, Hostetter JM (2015): Alimentary system. 1–257. In: Maxie MG (Ed), Jubb, Kennedy, and Palmer’s pathology of domestic animals, Volume 2, 6th ed. Elsevier, St. Louis, Missouri.
19. Yener Z, Sağlam YS, Timurkaan N, et al (2005): Immunohistochemical detection of parainfluenza type 3 virus antigens in paraffin sections of pneumonic caprine lungs. J Vet Med A Physiol Pathol Clin Med, 52, 268–271.