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Birecik (Türkiye)’te yaşayan Kelaynak popülasyonunun genetik yapısının mtDNA COI dizileri kullanılarak belirlenmesi

Yıl 2023, Cilt: 16 Sayı: 1, 31 - 37, 15.04.2023

Arif Parmaksız , Akin Yığın , Faruk Bozkaya

https://doi.org/10.46309/biodicon.2022.1201564

Öz

İnsan nüfusunun hızla artması şehirleşmenin genişlemesine ve gıdaya olan ihtiyacın gün geçtikçe artmasına neden olmaktadır. Bu ihtiyaçlar birçok tarımsal faaliyetin oluşmasına yol açtığı için doğal ve yerli türlerin habitatlarını değiştirmektedir. Bu habitat değişimi özellikle hassas türlerin beslenme ve üreme alanlarının azalmasına neden olduğu için bu türlere ait popülasyonların azalmasına hatta yok olmasına neden olabilmektedir. Son yıllarda Birecik ilçesinde yaşayan ve nesli tehlike altında olan Kelaynak (Geronticus eremita) kuş türü hassas türlerden biri olup, koruma altına alınmıştır. Bu çalışmanın amacı, Birecik’te yaşayan ve koruma altına alınan bireylerin oluşturduğu popülasyona ait genetik yapının mtDNA COI bölgesine dayalı genetik analizinin yapılmasıdır. Bu bireylere ait tüylerden DNA izolasyonu yapılmış ve elde edilen total DNA’lar kullanılarak hedef mtDNA COI gen bölgesi çoğaltılmıştır. Daha sonra dizi analizleri yapılarak genetik yapı belirlenmiş ve gen bankasındaki sekanslar ile karşılaştırılarak analizler yapılmıştır. Çalışılan tüm bireylerin mtDNA COI gen bölgesine ait herhangi bir varyasyon tespit edilmemiştir. Gen bankasındaki dizilerle karşılaştırma yapılmış, filogenetik ağaç çizilmiş ve sonuçlar karşılaştırılarak haplotipler değerlendirilmiştir.

Anahtar Kelimeler

kelaynak genetik varyasyon Geronticus eremita Haplotip mtDNA COI

Kaynakça

  • [1] Silvestro, D., Goria, S., Sterner, T., & Antonelli, A. (2022). Improving biodiversity protection through artificial intelligence. Nature sustainability, 5(5), 415-424.
  • [2] BirdLife International (2018). State of the world’s birds: taking the pulse of the planet, Cambridge, UK: BirdLife International.
  • [3] Rosenberg, K. V., Dokter, A. M., Blancher, P. J., …& Marra, P. P. (2019). Decline of the North American avifauna. Science, 366(6461): 120–124.
  • [4] Yilmaz, B., Demircioglu, I., & Korkmaz, D. (2021). Macroanatomic, light and scanning electron microscopic structure of the pecten oculi in northern bald ibis (Geronticus eremita). Anatomia, Histologia, Embryologia, 2021;50: 73-378.
  • [5] Böhm, C., Bowden, C. G., Seddon, P. J., Hatipoğlu, T., Oubrou, W., El Bekkay, M., ... & Unsöld, M. (2021). The northern bald ibis Geronticus eremita: history, current status and future perspectives. Oryx, 55(6), 934-946.
  • [6] Kılıç, A., &Uysal, E. (2021). Incubation Sharıng Of Northern Bald Ibıs Partners.Middle East Journal of Science, 7(2),167-181.
  • [7] Bowden, C. G., Aghnaj, A., Smith, K. W., & Ribi, M. (2003). The status and recent breeding performance of the critically endangered Northern Bald Ibis Geronticus eremita population on the Atlantic coast of Morocco. Ibis, 145(3), 419-431.
  • [8] Bowden, C. G., Smith, K. W., El Bekkay, M., Oubrou, W., Aghnaj, A., & Jimenez-Armesto, M. (2008). Contribution of research to conservation action for the Northern Bald Ibis Geronticus eremita in Morocco. Bird Conservation International, 18(S1), 74-S90.
  • [9] Kılıç, A., & Uysal, E. (2015). Kelaynak Kuşlarının (Geronticus eremita) Türkiye’deki üreme başarısı – 2013. Dicle Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 4(2), 87-94.
  • [10] Böhm, C., & Pegoraro, K. (2011). Der Waldrapp. Die Neue Brehmbücherei 659. Westarp, Hohenwarsleben.
  • [11] Bowden, C.G.R. (2015). International Single Species Action Plan for the Conservation of the Northern Bald Ibis (Geronticus eremita). AEWA Technical Series, No. 55. Bonn.
  • [12] Tel, O. Y., Bozkaya, F., & Keskin, O. (2013). Salmonella, Campylobacter, and Chlamydophila in bald ibis (Geronticus eremita) feces in Turkey. Journal of Zoo and Wildlife Medicine, 44(1), 21-26.
  • [13] Parmaksız, A. (2020). Population Genetic Diversity of Yellow Barbell (Carasobarbus luteus) from Kueik, Euphrates and Tigris Rivers Based on Mitochondrial DNA D-loop Sequences. Turkish Journal of Fisheries and Aquatic Sciences, 20(1), 79-86.
  • [14] Frankham, R., Ballou, J.D., & Briscoe, D.A. (2010). Introduction to conservation genetics. 2nd ed. New York, Cambridge University Press.
  • [15] Reed, D.H., Frankham, R. (2003). Correlation between fitness and genetic diversity. Conservation Biology, 17(1), 230–7.
  • [16] Vandewoestijne, S., Schtickzelle, N., & Baguette, M. (2008). Positive correlation between genetic diversity and fitness in a large, well-connected metapopulation. BMC Biology. 6, 46.
  • [17] Manel, S., Guerin, P. E., Mouillot, D., Blanchet, S., Velez, L., Albouy, C., & Pellissier, L. (2020). Global determinants of freshwater and marine fish genetic diversity. Nature communications. 11 (1): 1–9.
  • [18] Frankham, R., Briscoe, D.A., & Ballou, J. D. (2002). Introduction to Conservation Genetics. Cambridge University Press.
  • [19] Spielman, D., Brook, B.W., & Frankham, R. (2004). Most species are not driven to extinction before genetic factors impact them. Proceedings of the National Academy of Sciences, 101, 15261e15264.
  • [20] Ryman, N. (1991). Conservation genetics considerations in fishery management. Journal of Fish Biology, 39, 211-224.
  • [21] Ward, R.D. (2000). Genetics in fisheries management. Hydrobiologia, 420, 191-201.
  • [22] Liu, G., & Zhou, L. (2016). Population genetic structure and molecular diversity of the red swamp crayfish in China based on mtDNA COI gene sequences. Mitochondrial DNA Part A, 28(6), 860-866.
  • [23] Xu, Z.H., Chen, J.L., Cheng, D.F., Liu, Y., & Eric, F. (2011). Genetic variation among the geographic population of the Grain Aphid, Sitobion avenae (Hemiptera: Aphididae) in China inferred from mitochondrial COI gene sequence. Agricultural Sciences in China, 10(7). 1041-1048.
  • [24] Folmer, O., Black, M., Hoeh, W., Lutz, R., & Vrijenhoek, R. (1994). DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3: 294–299.
  • [25] Pegoraro, K., Föger, M., & Parson, W. (2001). First evidence of mtDNA sequence differences between Northern Bald Ibises (Geronticus eremita) of Moroccan and Turkish origin. Journal für Ornithologie, 142(4), 425-428.
  • [26] Pegoraro, K., Föger, M., & Parson, W. (2001). Mitochondrial DNA sequence evidence for close relationship of Bald Ibis, Geronticus calvus, and Waldrapp Ibis, G. eremita. Ostrich-Journal of African Ornithology, 72(3-4), 215-216.
  • [27] Boev, Z., Gradev, G., Klisurova, H., Klisurov, I., & Petrov, R. (2020). First record of the northern bald ibis Geronticus eremita (Linnaeus, 1758) in Bulgaria. Historia Naturalis Bulgarica, 41(3), 23-26.
  • [28] Demir, A. (2021). The impacts of climate change on genetic diversity. Biyolojik Çeşitlilik ve Koruma, 14(3), 511-518.

Determination of genetic structure of Bald ibis population living in Birecik (Turkey) using mtDNA COI sequences

Yıl 2023, Cilt: 16 Sayı: 1, 31 - 37, 15.04.2023

Arif Parmaksız , Akin Yığın , Faruk Bozkaya

https://doi.org/10.46309/biodicon.2022.1201564

Öz

The rapid increase in the human population causes the expansion of urbanization and the need for food to increase day by day. As these needs lead to the formation of many agricultural activities, they change the habitats of natural and native species. Since this habitat change causes a decrease in the feeding and breeding areas of especially sensitive species, it may lead to the decrease or even extinction of the populations of these species. The endangered bald ibis (Geronticus eremita), bird species living in Birecik district, is one of the sensitive species and has been taken under protection in recent years. The endangered bald ibis (Geronticus eremita) bird species living in Birecik district in recent years is one of the sensitive species and has been taken under protection. The aim of this study is to perform genetic analysis of the genetic structure of the protected individuals, which live in Birecik, based on the mtDNA COI region. DNA was isolated from the feathers of these individuals, and the target mtDNA COI gene region was amplified by using the total DNAs obtained. Then, the genetic structure was determined by sequence analysis, and the analyzes were made by comparing them with the sequences in the gene bank. No variation was detected in the mtDNA COI gene region of all studied individuals. Comparisons were made with the sequences in the gene bank, a phylogenetic tree was drawn, and haplotypes were evaluated by comparing the results

Anahtar Kelimeler

Bald ibis, genetic variation haplotype Geronticus eremita mtDNA COI

Kaynakça

  • [1] Silvestro, D., Goria, S., Sterner, T., & Antonelli, A. (2022). Improving biodiversity protection through artificial intelligence. Nature sustainability, 5(5), 415-424.
  • [2] BirdLife International (2018). State of the world’s birds: taking the pulse of the planet, Cambridge, UK: BirdLife International.
  • [3] Rosenberg, K. V., Dokter, A. M., Blancher, P. J., …& Marra, P. P. (2019). Decline of the North American avifauna. Science, 366(6461): 120–124.
  • [4] Yilmaz, B., Demircioglu, I., & Korkmaz, D. (2021). Macroanatomic, light and scanning electron microscopic structure of the pecten oculi in northern bald ibis (Geronticus eremita). Anatomia, Histologia, Embryologia, 2021;50: 73-378.
  • [5] Böhm, C., Bowden, C. G., Seddon, P. J., Hatipoğlu, T., Oubrou, W., El Bekkay, M., ... & Unsöld, M. (2021). The northern bald ibis Geronticus eremita: history, current status and future perspectives. Oryx, 55(6), 934-946.
  • [6] Kılıç, A., &Uysal, E. (2021). Incubation Sharıng Of Northern Bald Ibıs Partners.Middle East Journal of Science, 7(2),167-181.
  • [7] Bowden, C. G., Aghnaj, A., Smith, K. W., & Ribi, M. (2003). The status and recent breeding performance of the critically endangered Northern Bald Ibis Geronticus eremita population on the Atlantic coast of Morocco. Ibis, 145(3), 419-431.
  • [8] Bowden, C. G., Smith, K. W., El Bekkay, M., Oubrou, W., Aghnaj, A., & Jimenez-Armesto, M. (2008). Contribution of research to conservation action for the Northern Bald Ibis Geronticus eremita in Morocco. Bird Conservation International, 18(S1), 74-S90.
  • [9] Kılıç, A., & Uysal, E. (2015). Kelaynak Kuşlarının (Geronticus eremita) Türkiye’deki üreme başarısı – 2013. Dicle Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 4(2), 87-94.
  • [10] Böhm, C., & Pegoraro, K. (2011). Der Waldrapp. Die Neue Brehmbücherei 659. Westarp, Hohenwarsleben.
  • [11] Bowden, C.G.R. (2015). International Single Species Action Plan for the Conservation of the Northern Bald Ibis (Geronticus eremita). AEWA Technical Series, No. 55. Bonn.
  • [12] Tel, O. Y., Bozkaya, F., & Keskin, O. (2013). Salmonella, Campylobacter, and Chlamydophila in bald ibis (Geronticus eremita) feces in Turkey. Journal of Zoo and Wildlife Medicine, 44(1), 21-26.
  • [13] Parmaksız, A. (2020). Population Genetic Diversity of Yellow Barbell (Carasobarbus luteus) from Kueik, Euphrates and Tigris Rivers Based on Mitochondrial DNA D-loop Sequences. Turkish Journal of Fisheries and Aquatic Sciences, 20(1), 79-86.
  • [14] Frankham, R., Ballou, J.D., & Briscoe, D.A. (2010). Introduction to conservation genetics. 2nd ed. New York, Cambridge University Press.
  • [15] Reed, D.H., Frankham, R. (2003). Correlation between fitness and genetic diversity. Conservation Biology, 17(1), 230–7.
  • [16] Vandewoestijne, S., Schtickzelle, N., & Baguette, M. (2008). Positive correlation between genetic diversity and fitness in a large, well-connected metapopulation. BMC Biology. 6, 46.
  • [17] Manel, S., Guerin, P. E., Mouillot, D., Blanchet, S., Velez, L., Albouy, C., & Pellissier, L. (2020). Global determinants of freshwater and marine fish genetic diversity. Nature communications. 11 (1): 1–9.
  • [18] Frankham, R., Briscoe, D.A., & Ballou, J. D. (2002). Introduction to Conservation Genetics. Cambridge University Press.
  • [19] Spielman, D., Brook, B.W., & Frankham, R. (2004). Most species are not driven to extinction before genetic factors impact them. Proceedings of the National Academy of Sciences, 101, 15261e15264.
  • [20] Ryman, N. (1991). Conservation genetics considerations in fishery management. Journal of Fish Biology, 39, 211-224.
  • [21] Ward, R.D. (2000). Genetics in fisheries management. Hydrobiologia, 420, 191-201.
  • [22] Liu, G., & Zhou, L. (2016). Population genetic structure and molecular diversity of the red swamp crayfish in China based on mtDNA COI gene sequences. Mitochondrial DNA Part A, 28(6), 860-866.
  • [23] Xu, Z.H., Chen, J.L., Cheng, D.F., Liu, Y., & Eric, F. (2011). Genetic variation among the geographic population of the Grain Aphid, Sitobion avenae (Hemiptera: Aphididae) in China inferred from mitochondrial COI gene sequence. Agricultural Sciences in China, 10(7). 1041-1048.
  • [24] Folmer, O., Black, M., Hoeh, W., Lutz, R., & Vrijenhoek, R. (1994). DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3: 294–299.
  • [25] Pegoraro, K., Föger, M., & Parson, W. (2001). First evidence of mtDNA sequence differences between Northern Bald Ibises (Geronticus eremita) of Moroccan and Turkish origin. Journal für Ornithologie, 142(4), 425-428.
  • [26] Pegoraro, K., Föger, M., & Parson, W. (2001). Mitochondrial DNA sequence evidence for close relationship of Bald Ibis, Geronticus calvus, and Waldrapp Ibis, G. eremita. Ostrich-Journal of African Ornithology, 72(3-4), 215-216.
  • [27] Boev, Z., Gradev, G., Klisurova, H., Klisurov, I., & Petrov, R. (2020). First record of the northern bald ibis Geronticus eremita (Linnaeus, 1758) in Bulgaria. Historia Naturalis Bulgarica, 41(3), 23-26.
  • [28] Demir, A. (2021). The impacts of climate change on genetic diversity. Biyolojik Çeşitlilik ve Koruma, 14(3), 511-518.
Toplam 28 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Koruma ve Biyolojik Çeşitlilik
Bölüm Araştırma Makaleleri
Yazarlar

Arif Parmaksız 0000-0003-0321-8198

Akin Yığın 0000-0001-9758-1697

Faruk Bozkaya 0000-0001-6423-8067

Erken Görünüm Tarihi 14 Nisan 2023
Yayımlanma Tarihi 15 Nisan 2023
Gönderilme Tarihi 9 Kasım 2022
Kabul Tarihi 15 Aralık 2022
Yayımlandığı Sayı Yıl 2023 Cilt: 16 Sayı: 1

Kaynak Göster

APA Parmaksız, A., Yığın, A., & Bozkaya, F. (2023). Birecik (Türkiye)’te yaşayan Kelaynak popülasyonunun genetik yapısının mtDNA COI dizileri kullanılarak belirlenmesi. Biological Diversity and Conservation, 16(1), 31-37. https://doi.org/10.46309/biodicon.2022.1201564
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❖ Biological Diversity and Conservation
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❖ Publication Start Date 2008
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