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In vitro Micropropagation of Summer Snowflake (Leucojum aestivum L.) from Bulb Scale and Immature Embryo Explants

Yıl 2025, Cilt: 34 Sayı: 1, 40 - 45

Cuma Karaoglu Sebahattin Ozcan

https://doi.org/10.38042/biotechstudies.1672715

Öz

Leucojum aestivum is an important medicinal and ornamental plant due to the its alkaloid contents and beautiful flowers. However, this species suffers from low propagation rate under natural conditions and is threatened by excessive collection from nature. In this study for the purpose of rapid in vitro propagation of L. aestivum, two and four bulb scale, and immature embryo explants were cultured on Murashige and Skoog (MS) medium containing different concentrations of 6-benzylaminopurine (BAP) and α-naphthaleneacetic acid (NAA). The concentration of plant growth regulators, their combinations, and explant type had major influence on in vitro bulblet regeneration. The highest mean number of bulblets per explant (6.17) on bulb scales was obtained from MS medium containing 1 mg/l BAP and 1 mg/l NAA after 9 months in culture. Bulblet regeneration was much lower on immature embryos than bulb scales, and the highest number of bulblets per embryo explant (2.27) was achieved on MS medium supplemented with 0.5 mg/l BAP and 4 mg/l NAA after 5 months of culture initiation. In vitro regenerated bulblets were successfully rooted on MS medium with 1 mg/l NAA and finally transferred to potting mixture for acclimatization to external conditions.

Anahtar Kelimeler

Tissue culture Shoot regeneration Bulblet formation Growth regulators Acclimatization

Kaynakça

  • Aasim, M., Khawar, K.M. & Özcan, S. (2008). In Vitro Regeneration of Red Squill Urginea Maritima (L.) Baker. Using Thidiazuron, Biotechnology & Biotechnological Equipment, 22, 925-928, https://doi.org/10.1080/13102818.2008.10817580
  • Abedinimazraeh, M. & Kalatehjari, S. (2021). In vitro regeneration of bulblet using two and four bulb-scales explants of summer snowflake (Leucojum aestivum L.). Ornamental Horticulture, 27, 221-231. https://doi.org/10.1590/2447-536x.v27i2.2226
  • Adkins, S.W., Kunanuvatchaidach, R., Gray, S.J. & Adkins, A.L. (1993). Effect of ethylene and culture environment on rice callus proliferation. Journal of Experimental Botany, 44, 1829-1835. https://doi.org/10.1093/jxb/44.12.1829
  • Barik, D.P., Mohapatra, U., & Chand, P.K. (2005). High frequency in vitro regeneration of Lathyrus sativus L. Biol Plant, 49, 637–639. https://doi.org/10.1007/s10535-005-0066-x
  • Bronsema, F., Van Oostveen, W. & Van Lammeren, A. (1997). Comparative analysis of callus formation and regeneration on cultured immature maize embryos of the inbred lines A188 and A632. Plant Cell, Tissue. and Organ. Culture, 50, 57–65. https://doi.org/10.1023/A:1005802828004
  • Çakmak, D., Karaoğlu, C., Aasim, M., Sancak, C. & Özcan, S. (2016). Advancement in protocol for in vitro seed germination, regeneration, bulblet maturation, and acclimatization of Fritillaria persica. Turkish Journal of Biology, 40, 878-888. https://doi.org/10.3906/biy-1510-18
  • Chatfield S.P., & Raizada, M.N., (2008). Ethylene and shoot regeneration: hookless1 modulates de novo shoot organogenesis in Arabidopsis thaliana. Plant Cell Rep., 27, 655–666. https://doi.org/10.1007/s00299-007-0496-3
  • Darlington, C.D., & Janaki-Ammal, E.K. (1946). Chromosome Atlas of Cultivated Plants. London: Allen and Unwin, Pp. 397.
  • Diop, M.F., Hehn, A., Ptak, A. et al. (2007). Hairy root and tissue cultures of Leucojum aestivum L. relationships to galanthamine content. Phytochem Rev 6, 137–141 (2007). https://doi.org/10.1007/s11101-006-9043-z
  • George, E.F., Hall, M.A. & Klerk, GJ.D. (2008). Plant Growth Regulators III : Gibberellins, Ethylene, Abscisic Acid, their Analogues and Inhibitors; Miscellaneous Compounds.. In: George, E.F., Hall, M.A. & Klerk, GJ.D. (eds) Plant Propagation by Tissue Culture. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5005-3_7
  • Khawar, K.M., Cocu, S., Parmaksiz, I., Sarihan, E.O., & Ozcan, S. (2005). Mass proliferation of Madonna lily (Lilium candidum L) under in vitro conditions. Pak J Bot, 37, 243-248.
  • Kohut, E., Ördögh, M., Jámbor-Benczúr, E., & Máthé, Á. (2007). Results with the establishment of in vitro culture of Leucojum aestivum. International Journal of Horticultural Science, 13, 67-71. https://doi.org/10.31421/IJHS/13/2/723
  • Mill, R.R. (1984). Leucojum L. In: Davis PH (Ed.). Flora of Turkey and the East Aegean Islands, Vol. 8, 364–365, Edinburgh University Press.
  • Mirici, S., Parmaksız, İ., Özcan, S. et al. (2005). Efficient in vitro bulblet regeneration from immature embryos of endangered Sternbergia fischeriana. Plant Cell Tiss Organ Cult, 80, 239–246. https://doi.org/10.1007/s11240-004-3016-y
  • Murashige, T. & Skoog, F. (1962). A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Plant Physiology, 15, 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  • Ochatt, S., Conreux, C., Moussa Mcolo, R. et al. (2018). Phytosulfokine-alpha, an enhancer of in vitro regeneration competence in recalcitrant legumes. Plant Cell Tiss Organ Cult, 135, 189–201. https://doi.org/10.1007/s11240-018-1455-0
  • Ozel, C.A., & Khawar, K.M. (2007). In vitro bulblet regeneration of Ornithogalum oligophyllum E. D. Clarke using twin scale bulb explants. Propagation of ornamental plants, 7, 82,88.
  • Ozel, C.A., Unal, F., & Khawar, K.M. (2007). In vitro axillary bulblet regeneration of Turkish yellow grape hyacinth Muscari macrocarpum Sweet from twin scale explants. Research Journal of Agriculture and Biological Sciences, 3, 924-929.
  • Ptak, A. & Cierniak, O. (2003) Regeneration of summer snowflake (Leucojum aestivum L.) in in vitro cultures. Biotechnologia, 634, 239-245
  • Ptak, A. (2014). Leucojum aestivum L. in vitro bulbs induction and acclimatization. Central European Journal of Biology, 9, 1011-1021. https://doi.org/10.2478/s11535-014-0339-5
  • Ptak, A., El Tahchy, A., Dupire, F., Boisbrun, M., Henry, M., Chapleur, Y., Mos, M., & Laurain-Mattar, D. (2009). LCMS and GCMS for the screening of alkaloids in natural and In vitro extracts of Leucojum aestivum. J Nat Prod. Jan, 72, 142-7. https://doi.org/10.1021/np800585c
  • Ptak, A., Tahchy, A., Skrzypek, E., Wójtowicz, T. & Laurain-Mattar, D. (2013). Influence of auxins on somatic embryogenesis and alkaloid accumulation in Leucojum aestivum callus. Open Life Sciences, 8, 591-599. https://doi.org/10.2478/s11535-013-0160-y
  • Ptak, A., Tahchy, A.E., Wyzgolik, G., Henry, M. & Laurain-Mattar, D. (2010). Effects of ethylene on somatic embryogenesis and galanthamine content in Leucojum aestivum L. cultures. Plant Cell Tiss Organ Cult, 102, 61–67. https://doi.org/10.1007/s11240-010-9706-8
  • Roy, P.K., Lodha, M.L. & Mehta, S.L. (2001). In vitro regeneration from internodal explants and somaclonal variation in chickpea (Cicer arietinum L). J. Plant Biochem. Biotechnol, 10, 107–112. https://doi.org/10.1007/BF03263118
  • Sevimay, C.S., Khawar, K.M., Parmaksiz, I., Cocu, S., Sancak, C., Sarihan, E.O., & Özcan, S. (2005). Prolific in vitro bulblet formation from bulb scales of meadow lily (Lilium candidum L.). Periodicum Biologorum, 107, 107-111.
  • Snedecor, G.W. & Cochran W.A. (1976). Statistical methods Iowa State University Press, Ames. Statistical methods. 7th Edition.
  • Tubives. (2024). Türkiye bitkileri veri servisi anasayfa [Online] http://www.tubives.com. [12.03.2024].
  • Uzun, S., Parmaksiz, I., Uranbey, S., Mirici, S., Sarihan, E. O., Ipek, A., Kaya, M. D., Gürbüz, B., Arslan, N., Sancak, C., Khawar, K.M., & Özcan, S. (2014). In vitro micropropagation from immature embryos of the endemic and endangered Muscari muscarimi Medik. Turkish Journal of Biology, 38, 83-88. https://doi.org/10.3906/biy-1307-52

Yıl 2025, Cilt: 34 Sayı: 1, 40 - 45

Cuma Karaoglu Sebahattin Ozcan

https://doi.org/10.38042/biotechstudies.1672715

Öz

Kaynakça

  • Aasim, M., Khawar, K.M. & Özcan, S. (2008). In Vitro Regeneration of Red Squill Urginea Maritima (L.) Baker. Using Thidiazuron, Biotechnology & Biotechnological Equipment, 22, 925-928, https://doi.org/10.1080/13102818.2008.10817580
  • Abedinimazraeh, M. & Kalatehjari, S. (2021). In vitro regeneration of bulblet using two and four bulb-scales explants of summer snowflake (Leucojum aestivum L.). Ornamental Horticulture, 27, 221-231. https://doi.org/10.1590/2447-536x.v27i2.2226
  • Adkins, S.W., Kunanuvatchaidach, R., Gray, S.J. & Adkins, A.L. (1993). Effect of ethylene and culture environment on rice callus proliferation. Journal of Experimental Botany, 44, 1829-1835. https://doi.org/10.1093/jxb/44.12.1829
  • Barik, D.P., Mohapatra, U., & Chand, P.K. (2005). High frequency in vitro regeneration of Lathyrus sativus L. Biol Plant, 49, 637–639. https://doi.org/10.1007/s10535-005-0066-x
  • Bronsema, F., Van Oostveen, W. & Van Lammeren, A. (1997). Comparative analysis of callus formation and regeneration on cultured immature maize embryos of the inbred lines A188 and A632. Plant Cell, Tissue. and Organ. Culture, 50, 57–65. https://doi.org/10.1023/A:1005802828004
  • Çakmak, D., Karaoğlu, C., Aasim, M., Sancak, C. & Özcan, S. (2016). Advancement in protocol for in vitro seed germination, regeneration, bulblet maturation, and acclimatization of Fritillaria persica. Turkish Journal of Biology, 40, 878-888. https://doi.org/10.3906/biy-1510-18
  • Chatfield S.P., & Raizada, M.N., (2008). Ethylene and shoot regeneration: hookless1 modulates de novo shoot organogenesis in Arabidopsis thaliana. Plant Cell Rep., 27, 655–666. https://doi.org/10.1007/s00299-007-0496-3
  • Darlington, C.D., & Janaki-Ammal, E.K. (1946). Chromosome Atlas of Cultivated Plants. London: Allen and Unwin, Pp. 397.
  • Diop, M.F., Hehn, A., Ptak, A. et al. (2007). Hairy root and tissue cultures of Leucojum aestivum L. relationships to galanthamine content. Phytochem Rev 6, 137–141 (2007). https://doi.org/10.1007/s11101-006-9043-z
  • George, E.F., Hall, M.A. & Klerk, GJ.D. (2008). Plant Growth Regulators III : Gibberellins, Ethylene, Abscisic Acid, their Analogues and Inhibitors; Miscellaneous Compounds.. In: George, E.F., Hall, M.A. & Klerk, GJ.D. (eds) Plant Propagation by Tissue Culture. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5005-3_7
  • Khawar, K.M., Cocu, S., Parmaksiz, I., Sarihan, E.O., & Ozcan, S. (2005). Mass proliferation of Madonna lily (Lilium candidum L) under in vitro conditions. Pak J Bot, 37, 243-248.
  • Kohut, E., Ördögh, M., Jámbor-Benczúr, E., & Máthé, Á. (2007). Results with the establishment of in vitro culture of Leucojum aestivum. International Journal of Horticultural Science, 13, 67-71. https://doi.org/10.31421/IJHS/13/2/723
  • Mill, R.R. (1984). Leucojum L. In: Davis PH (Ed.). Flora of Turkey and the East Aegean Islands, Vol. 8, 364–365, Edinburgh University Press.
  • Mirici, S., Parmaksız, İ., Özcan, S. et al. (2005). Efficient in vitro bulblet regeneration from immature embryos of endangered Sternbergia fischeriana. Plant Cell Tiss Organ Cult, 80, 239–246. https://doi.org/10.1007/s11240-004-3016-y
  • Murashige, T. & Skoog, F. (1962). A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Plant Physiology, 15, 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  • Ochatt, S., Conreux, C., Moussa Mcolo, R. et al. (2018). Phytosulfokine-alpha, an enhancer of in vitro regeneration competence in recalcitrant legumes. Plant Cell Tiss Organ Cult, 135, 189–201. https://doi.org/10.1007/s11240-018-1455-0
  • Ozel, C.A., & Khawar, K.M. (2007). In vitro bulblet regeneration of Ornithogalum oligophyllum E. D. Clarke using twin scale bulb explants. Propagation of ornamental plants, 7, 82,88.
  • Ozel, C.A., Unal, F., & Khawar, K.M. (2007). In vitro axillary bulblet regeneration of Turkish yellow grape hyacinth Muscari macrocarpum Sweet from twin scale explants. Research Journal of Agriculture and Biological Sciences, 3, 924-929.
  • Ptak, A. & Cierniak, O. (2003) Regeneration of summer snowflake (Leucojum aestivum L.) in in vitro cultures. Biotechnologia, 634, 239-245
  • Ptak, A. (2014). Leucojum aestivum L. in vitro bulbs induction and acclimatization. Central European Journal of Biology, 9, 1011-1021. https://doi.org/10.2478/s11535-014-0339-5
  • Ptak, A., El Tahchy, A., Dupire, F., Boisbrun, M., Henry, M., Chapleur, Y., Mos, M., & Laurain-Mattar, D. (2009). LCMS and GCMS for the screening of alkaloids in natural and In vitro extracts of Leucojum aestivum. J Nat Prod. Jan, 72, 142-7. https://doi.org/10.1021/np800585c
  • Ptak, A., Tahchy, A., Skrzypek, E., Wójtowicz, T. & Laurain-Mattar, D. (2013). Influence of auxins on somatic embryogenesis and alkaloid accumulation in Leucojum aestivum callus. Open Life Sciences, 8, 591-599. https://doi.org/10.2478/s11535-013-0160-y
  • Ptak, A., Tahchy, A.E., Wyzgolik, G., Henry, M. & Laurain-Mattar, D. (2010). Effects of ethylene on somatic embryogenesis and galanthamine content in Leucojum aestivum L. cultures. Plant Cell Tiss Organ Cult, 102, 61–67. https://doi.org/10.1007/s11240-010-9706-8
  • Roy, P.K., Lodha, M.L. & Mehta, S.L. (2001). In vitro regeneration from internodal explants and somaclonal variation in chickpea (Cicer arietinum L). J. Plant Biochem. Biotechnol, 10, 107–112. https://doi.org/10.1007/BF03263118
  • Sevimay, C.S., Khawar, K.M., Parmaksiz, I., Cocu, S., Sancak, C., Sarihan, E.O., & Özcan, S. (2005). Prolific in vitro bulblet formation from bulb scales of meadow lily (Lilium candidum L.). Periodicum Biologorum, 107, 107-111.
  • Snedecor, G.W. & Cochran W.A. (1976). Statistical methods Iowa State University Press, Ames. Statistical methods. 7th Edition.
  • Tubives. (2024). Türkiye bitkileri veri servisi anasayfa [Online] http://www.tubives.com. [12.03.2024].
  • Uzun, S., Parmaksiz, I., Uranbey, S., Mirici, S., Sarihan, E. O., Ipek, A., Kaya, M. D., Gürbüz, B., Arslan, N., Sancak, C., Khawar, K.M., & Özcan, S. (2014). In vitro micropropagation from immature embryos of the endemic and endangered Muscari muscarimi Medik. Turkish Journal of Biology, 38, 83-88. https://doi.org/10.3906/biy-1307-52
Toplam 28 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Tarımda Bitki Biyoteknolojisi
Bölüm Research Articles
Yazarlar

Cuma Karaoglu 0000-0001-6504-1696

Sebahattin Ozcan 0000-0001-5127-1482

Erken Görünüm Tarihi 9 Nisan 2025
Yayımlanma Tarihi
Gönderilme Tarihi 15 Mayıs 2024
Kabul Tarihi 8 Nisan 2025
Yayımlandığı Sayı Yıl 2025 Cilt: 34 Sayı: 1

Kaynak Göster

APA Karaoglu, C., & Ozcan, S. (2025). In vitro Micropropagation of Summer Snowflake (Leucojum aestivum L.) from Bulb Scale and Immature Embryo Explants. Biotech Studies, 34(1), 40-45. https://doi.org/10.38042/biotechstudies.1672715


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