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Assessment of Antibacterial and Antifungal Activities of Ethanolic Flower Extracts from Rosa damascena against Pathogenic Micro-Organisms

Yıl 2025, Cilt: 8 Sayı: 3, 304 - 322, 15.05.2025

Parwiz Niazi , Abdul Bari Hejran , Obaidullah Alimyar

https://doi.org/10.47115/bsagriculture.1583192

Öz

This study investigates the antimicrobial potential of Rosa damascena flower extract, with a focus on its antibacterial and antifungal properties. The primary objective was to assess the inhibitory activity of the ethanolic extract against a spectrum of bacterial and fungal pathogens. Using the agar disc diffusion method, the extract was evaluated at a concentration of 100 µg/ml against two Gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus), one Gram-negative bacterium (Escherichia coli), and four fungal strains (Aspergillus fumigatus AF293, Aspergillus niger ATCC 16404, Candida albicans SC5314, and Monascus purpureus ATCC 1008). The zones of inhibition produced by the extract were compared to those of standard antibiotics: ciprofloxacin for antibacterial activity and fluconazole for antifungal activity. The findings revealed significant antibacterial effects, particularly against Gram-positive bacteria, with clear zones of inhibition, suggesting that Rosa damascena harbors a diverse array of bioactive secondary metabolites, the extract demonstrated notable antifungal activity, with inhibition observed across several fungal strains. These results underscore the extract's promising antimicrobial potential, highlighting its efficacy as a source of bioactive compounds, the study suggests that Rosa damascena could serve as a valuable resource for the development of novel antimicrobial agents, particularly in light of increasing resistance to conventional antibiotics. Further investigation into the specific mechanisms of action, toxicity, and the isolation of active compounds is warranted to advance its potential as a therapeutic agent in the pharmaceutical industry. The synergistic effects with other natural compounds could enhance its therapeutic efficacy and expand its potential applications in clinical settings.

Anahtar Kelimeler

Rosa damascene Ethanolic extract Antimicrobial activity Minimum inhibitory concentration

Kaynakça

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  • Abdel-Nasser A, Badr AN, Fathy HM, Ghareeb MA, Barakat OS, Hathout AS. 2024. Antifungal, antiaflatoxigenic, and cytotoxic properties of bioactive secondary metabolites derived from Bacillus species. Sci Rep, 14(1): 16590. https://doi.org/10.1038/s41598-024-21204-1
  • Acharya T, Hare J. 2022. Sabouraud agar and other fungal growth media. In: Laboratory protocols in fungal biology: Current methods in fungal biology. Cham: Springer Int Publ, London, UK, pp: 69-86. https://doi.org/10.1007/978-3-030-83793-5_6
  • Akram M, Riaz M, Munir N, Akhter N, Zafar S, Jabeen F. 2020. Chemical constituents, experimental and clinical pharmacology of Rosa damascena: A literature review. J Pharm Pharmacol, 72(2): 161-174. https://doi.org/10.1111/jphp.13144
  • Amirullah NA, Abidin NZ, Abdullah N, Manickam S. 2021. Application of ultrasound towards improving the composition of phenolic compounds and enhancing in vitro bioactivities of Pleurotus pulmonarius (Fr.) Quél extracts. Biocatal Agric Biotechnol, 31: 101881. https://doi.org/10.1016/j.bcab.2021.101881
  • Andrews JM. 2001. Determination of minimum inhibitory concentrations. J Antimicrob Chemother, 48(suppl_1): 5-16. https://doi.org/10.1093/jac/48.suppl_1.5
  • Anokwuru CP, Anyasor GN, Ajibaye O, Fakoya O, Okebugwu P. 2011. Effect of extraction solvents on phenolic, flavonoid, and antioxidant activities of three Nigerian medicinal plants. Nat Sci, 9(7): 53-61. https://doi.org/10.7537/marsnsj090711.09
  • Antoniadou M, Rozos G, Vaou N, Zaralis K, Ersanli C, Alexopoulos A. 2024. Comprehensive bio-screening of phytochemistry and biological capacity of Origanum vulgare and Salvia triloba extracts against oral cariogenic and food-origin pathogenic bacteria. Biomolecules, 14(6): 619. https://doi.org/10.3390/biom14060619
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Assessment of Antibacterial and Antifungal Activities of Ethanolic Flower Extracts from Rosa damascena against Pathogenic Micro-Organisms

Yıl 2025, Cilt: 8 Sayı: 3, 304 - 322, 15.05.2025

Parwiz Niazi , Abdul Bari Hejran , Obaidullah Alimyar

https://doi.org/10.47115/bsagriculture.1583192

Öz

This study investigates the antimicrobial potential of Rosa damascena flower extract, with a focus on its antibacterial and antifungal properties. The primary objective was to assess the inhibitory activity of the ethanolic extract against a spectrum of bacterial and fungal pathogens. Using the agar disc diffusion method, the extract was evaluated at a concentration of 100 µg/ml against two Gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus), one Gram-negative bacterium (Escherichia coli), and four fungal strains (Aspergillus fumigatus AF293, Aspergillus niger ATCC 16404, Candida albicans SC5314, and Monascus purpureus ATCC 1008). The zones of inhibition produced by the extract were compared to those of standard antibiotics: ciprofloxacin for antibacterial activity and fluconazole for antifungal activity. The findings revealed significant antibacterial effects, particularly against Gram-positive bacteria, with clear zones of inhibition, suggesting that Rosa damascena harbors a diverse array of bioactive secondary metabolites, the extract demonstrated notable antifungal activity, with inhibition observed across several fungal strains. These results underscore the extract's promising antimicrobial potential, highlighting its efficacy as a source of bioactive compounds, the study suggests that Rosa damascena could serve as a valuable resource for the development of novel antimicrobial agents, particularly in light of increasing resistance to conventional antibiotics. Further investigation into the specific mechanisms of action, toxicity, and the isolation of active compounds is warranted to advance its potential as a therapeutic agent in the pharmaceutical industry. The synergistic effects with other natural compounds could enhance its therapeutic efficacy and expand its potential applications in clinical settings.

Anahtar Kelimeler

Rosa damascene Ethanolic extract Antimicrobial activity Minimum inhibitory concentration

Kaynakça

  • Abdel-Malek AR, Moustafa AY, Salem SH. 2024. Antimicrobial and cytotoxic activities of flavonoid and phenolics extracted from Sepia pharaonis ink (Mollusca: Cephalopoda). BMC Biotechnol, 24(1): 54. https://doi.org/10.1186/s12896-024-02005-0
  • Abdel-Nasser A, Badr AN, Fathy HM, Ghareeb MA, Barakat OS, Hathout AS. 2024. Antifungal, antiaflatoxigenic, and cytotoxic properties of bioactive secondary metabolites derived from Bacillus species. Sci Rep, 14(1): 16590. https://doi.org/10.1038/s41598-024-21204-1
  • Acharya T, Hare J. 2022. Sabouraud agar and other fungal growth media. In: Laboratory protocols in fungal biology: Current methods in fungal biology. Cham: Springer Int Publ, London, UK, pp: 69-86. https://doi.org/10.1007/978-3-030-83793-5_6
  • Akram M, Riaz M, Munir N, Akhter N, Zafar S, Jabeen F. 2020. Chemical constituents, experimental and clinical pharmacology of Rosa damascena: A literature review. J Pharm Pharmacol, 72(2): 161-174. https://doi.org/10.1111/jphp.13144
  • Amirullah NA, Abidin NZ, Abdullah N, Manickam S. 2021. Application of ultrasound towards improving the composition of phenolic compounds and enhancing in vitro bioactivities of Pleurotus pulmonarius (Fr.) Quél extracts. Biocatal Agric Biotechnol, 31: 101881. https://doi.org/10.1016/j.bcab.2021.101881
  • Andrews JM. 2001. Determination of minimum inhibitory concentrations. J Antimicrob Chemother, 48(suppl_1): 5-16. https://doi.org/10.1093/jac/48.suppl_1.5
  • Anokwuru CP, Anyasor GN, Ajibaye O, Fakoya O, Okebugwu P. 2011. Effect of extraction solvents on phenolic, flavonoid, and antioxidant activities of three Nigerian medicinal plants. Nat Sci, 9(7): 53-61. https://doi.org/10.7537/marsnsj090711.09
  • Antoniadou M, Rozos G, Vaou N, Zaralis K, Ersanli C, Alexopoulos A. 2024. Comprehensive bio-screening of phytochemistry and biological capacity of Origanum vulgare and Salvia triloba extracts against oral cariogenic and food-origin pathogenic bacteria. Biomolecules, 14(6): 619. https://doi.org/10.3390/biom14060619
  • Azizi A, Mahboob M, Monib AW, Hassand MH, Sediqi S, Niazi P. 2023. The role of plants in human health. Br J Biol Stud, 3(1): 8-12. https://doi.org/10.1234/bjbs.2023.0301
  • Bari LR, Nazari R, Amiri S. 2024. Rosa. In Edible flowers. Academic Press, Cambridge, USA, pp: 231-247.
  • Bhadange YA, Carpenter J, Saharan VK. 2024. A comprehensive review on advanced extraction techniques for retrieving bioactive components from natural sources. ACS Omega, 9(29): 31274-31297. https://doi.org/10.1021/acsomega.4c01639
  • Bi S, Srivastava R, Ahmad T. 2024. The potential antifungal activity of the developed palladium nanoparticles. Biom Pharm J, 17(4).
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Toplam 79 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Gıda Mühendisliği
Bölüm Research Articles
Yazarlar

Parwiz Niazi 0009-0006-9628-8337

Abdul Bari Hejran 0009-0000-0443-0305

Obaidullah Alimyar 0009-0009-3253-2371

Yayımlanma Tarihi 15 Mayıs 2025
Gönderilme Tarihi 12 Kasım 2024
Kabul Tarihi 15 Ocak 2025
Yayımlandığı Sayı Yıl 2025 Cilt: 8 Sayı: 3

Kaynak Göster

APA Niazi, P., Hejran, A. B., & Alimyar, O. (2025). Assessment of Antibacterial and Antifungal Activities of Ethanolic Flower Extracts from Rosa damascena against Pathogenic Micro-Organisms. Black Sea Journal of Agriculture, 8(3), 304-322. https://doi.org/10.47115/bsagriculture.1583192
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