Öz
Morus Nigra’nın Fare Nöroblastoma Hücre Kültür Dizinindeki Nöroprotektif Etkinliğinin Araştırılması
D. Gülce TATLI1, Kâmil VURAL1Yeşim Turhan ÖZCAN2, Ertan DARIVERENLİ1
1 Manisa Celal Bayar Üniversitesi Farmakoloji /Türkiye
2 İzmir Şehir Hastanesi Çocuk ve Ergen Psikiyatri /Türkiye
E-mail: D. Gülce TATLI: dgulceuyar@hotmail.com
Kâmil VURAL: kfsvural@yahoo.com
Yeşim Turhan ÖZCAN: yesimturhanozcan@gmail.com
Ertan Darıverenli: ertan6876@gmail.com
*Sorumlu Yazar / Corresponding Author: D. Gülce TATLI
Gönderim Tarihi / Received:
Kabul Tarihi / Accepted:
DOI: 10.34087/cbusbed.
Öz
Giriş ve Amaç: Morus Nigra (Moraceae), insanlar tarafından sıkça tüketilen bir meyvedir. Aynı zamanda geleneksel tıpta çeşitli hastalıkların tedavisinde antialerjik, antienflamatuvar, antibakteriyel ve antikanser özellikleri olduğu için kullanıla gelmektedir. Morus Nigra ektresinin hücre canlılığı üzerine etkileri olduğu bazı kaynaklarda ise bu etkisinin olmadığı belirtilmiştir. Bu nedenle Morus Nigra ektresinin Nb2a hücre hattında nörotoksisite, apoptoz ve hücre canlılığı üzerindeki etkilerini incelemek amacıyla yapılmıştır.
Gereç ve Yöntemler: Morus Nigra’nın metanol ekstratları, farklı konsantrasyonlarda (1, 3, 10, 30, 100, 300 ve 1000 μg/ml) Nb2a hücre hattına uygulanmıştır. Nörotoksisite tarama testi (NTT), annexin V yöntemiyle apoptoz tespiti ve hücre canlılık/proliferasyon testleri kullanılarak hücre üzerindeki etkiler değerlendirilmiştir.
Bulgular: Morus Nigra'nın metanol ekstratlarının hücre canlılığı ve apoptozis üzerinde belirgin bir etki göstermediğini, ancak nörit uzaması üzerine 100 ve 300 µg/ml konsantrasyonlarda ılımlı nöroprotektif etki gösterdiği ortaya koymuştur.
Sonuç: Bu bulgular, Morus Nigra'nın antikanser özellikleri olduğu düşünülmemekle birlikte içerdiği fenolik bileşiklerin antioksidan etkileri nedeniyle nöronal sistemdeki aksonları koruyabileceği gösterilmiştir.
Anahtar kelimeler: Morus Nigra, Hücre kültürü, Nb2a, apopitozis, NTT
Abstract
Aim; Morus Nigra (Moraceae) is a fruit frequently consumed by humans. It is also used in traditional medicine to treat various diseases because it has antiallergic, anti-inflammatory, antibacterial and anticancer properties. It has been stated that Morus Nigra extract has effects on cell viability and some sources do not have this effect. Therefore, this study was carried out to investigate the effects of Morus Nigra extract on neurotoxicity, apoptosis and cell viability in Nb2a cell line
Method: Methanol extracts of Morus Nigra were applied to Nb2a cell line at different concentrations (1, 3, 10, 30, 100, 300 and 1000 μg/ml). The effects on the cells were evaluated using neurotoxicity screening test (NTT), apoptosis detection by annexin V method and cell viability/proliferation tests.
Results: It was found that methanol extracts of Morus Nigra had no significant effect on cell viability and apoptosis, but showed moderate neuroprotective effect on neurite elongation at concentrations of 100 and 300 µg/ml.
Conclusion: These findings indicate that although Morus Nigra is not thought to have anticancer properties, it has been shown that the phenolic compounds it contains may protect axons in the neuronal system due to their antioxidant effects.
Keywords: Morus Nigra, Cell culture, Nb2a, apoptosis, NTT
Anahtar Kelimeler
Etik Beyan
Manisa Celal Bayar Üniversitesi Bilimsel Etik komisyonu 15.03.2023 taihinde 20.478.486 1751 sayılı karırı ile Etik kurul onayı Onayı verilmiştir
Kaynakça
- 1. Gundogdu M, Muradoglu F, Sensoy RIG et al Determination of fruit chemical properties of Morus nigra L., Morus alba L. and Morus rubra L. by HPLC. Sci Hortic 132:37–41. doi:10.1016/j.scienta.2011.09.035
- 2. Ozgen M., Serce S., Kaya C. Phytochemical and antioxidant properties of anthocyanin-rich Morus nigra and Morus rubra fruits. Scientia Horticulturae. 2009;119(3):275–279. doi: 10.1016/j.scienta.2008.08.007.
- 3. Souza G. R., Oliveira-Junior R. G., Diniz T. C., et al. Assessment of the antibacterial, cytotoxic and antioxidant activities of Morus nigra L. (Moraceae) Brazilian Journal of Biology. 2018;78(2):248–254. doi: 10.1590/1519-6984.05316.
- 4. Padilha M. M., Vilela F. C., Rocha C. Q., et al. Antiinflammatory properties of Morus nigra leaves. Phytotherapy Research. 2010; 24(10):1496–1500. doi: 10.1002/ptr.3134.]
- 5. Chen H., Pu J., Liu D., et al. Anti-inflammatory and antinociceptive properties of flavonoids from the fruits of black mulberry (Morus nigra L.) PLoS One. 2016;11(4): p. e0153080. doi: 10.1371/journal.pone.0153080.
- 6. Van Hung P. Phenolic Compounds of Cereals and Their Antioxidant Capacity. Critical Reviews in Food Science and Nutrition, 2016 56 (1): 25-35.
- 7. Cheatham, C.L.; Canipe, L.G., 3rd; Millsap, G.; Stegall, J.M.; Chai, S.C.; Sheppard, K.W.; Lila, M.A. Six-month intervention with wild blueberries improved speed of processing in mild cognitive decline: A double-blind, placebo-controlled, randomized clinical trial. Nutr. Neurosci. 2022, 1–15
- 8. Cheatham, C.L.; Nieman, D.C.; Neilson, A.P.; Lila, M.A. Enhancing the Cognitive Effects of Flavonoids with Physical Activity: Is There a Case for the Gut Microbiome? Front. Neurosci. 2022, 16, 833202.
- 9. Lau, F.C.; Shukitt-Hale, B.; Joseph, J.A. Nutritional intervention in brain aging: Reducing the effects of inflammation and oxidative stress. Subcell. Biochem. 2007, 42, 299–318.
- 10. Albarracin, S.L.; Stab, B.; Casas, Z.; Sutachan, J.J.; Samudio, I.; Gonzalez, J.; Gonzalo, L.; Capani, F.; Morales, L.; Barreto, G.E. Effects of natural antioxidants in neurodegenerative disease. Nutr. Neurosci. 2012, 15, 1–9.]
- 11. Akter, R.; Rahman, H.; Behl, T.; Chowdhury, M.A.R.; Manirujjaman, M.; Bulbul, I.J.; Elshenaw, S.E.; Tit, D.M.; Bungau, S. Prospective Role of Polyphenolic Compounds in the Treatment of Neurodegenerative Diseases. CNS Neurol. Disord. Drug Targets 2021, 20, 430–450.
- 12. Mattioli R, Francioso A, Mosca L, Silva P. Anthocyanins: A Comprehensive Review of Their Chemical Properties and Health Effects on Cardiovascular and Neurodegenerative Diseases. Molecules. 2020 Aug 21;25(17):3809. doi: 10.3390/molecules25173809.
- 13. Shahneh F.Z., Baradaran B., Majidi J., Babaloo Z. Echinophora platyloba DC (Apiaceae) crude extract induces apoptosis in human prostate adenocarcinoma cells (PC-3) Biomed. J. 2014; 37:298–304. doi: 10.4103/2319-4170.125653
- 14. Vural K., Seyrek O. The Neuroprotective Effect of Pioglitazone on NB2a Mouse Neuroblastoma Cell Culture. Kafkas Univ Vet Fak Derg, 25 (1): 1-8, 2019. DOI: 10.9775/kvfd.2018.19940
- 15. Tatlı G., Vural K., Darıverenli E. Nöropeptid Y'nin fare nöroblastoma (NB2A) hücre kültür dizinindeki nöroprotektif etkinliğinin araştırılması 2023 Tıpta Uzmanlık Tezi
- 16. Khalid N., Fawad S.A., Ahmed I. Antimicrobial activity, phytochemical profile and trace minerals of black mulberry (Morus nigra L.) fresh juice. Pak. J. Bot. 2011; 43:91–96
- 17. Ramos S Cancer chemoprevention and chemotherapy. Dietary polyphenols and signalling pathways. Mol. Nutr. Food, 2008 Res.52: 507-526.
- 18. Huang WU, Cai ZY and Zhang J Natural phenolic compounds from medicinal herbs and dietary plants: potential use for cancer prevention. Nutr. Cancer , 2010 62 (1): 1- 20.
- 19. Borbalan AMA, Zorro L, Gullen DA and Barroso CG Study of the polyphenol content of red and white grape varieties by liquid chromatographymass spectrometry and its retalionship to antioxidant power. J. Chrom. A, 2003 1012: 31-38
- 20. Çakıroğlu, E.; Uysal, T.; Çalıbaşı Koçal, G.; Aygenli, F.; Baran, G.; Baskın, Y. The role of Morus nigra extract and its active compounds as drug candidate on human colorectal adenocarcinoma cell line HT-29. Int. J. Clin. Oncol. Cancer Res. 2017, 2, 10–14
- 21. Qadir, M.I.; Ali, M.; Ibrahim, Z. Anticancer activity of Morus nigra leaves extract. Bangladesh J. Pharmacol. 2014, 9, 496–497
- 22. Turan, I.; Demir, S.; Kilinc, K.; Burnaz, N.A.; Yaman, S.O.; Akbulult, K.; Mentese, A.; Aliyazicioglu, Y.; Deger, O. Antiproliferative and apoptotic effect of Morus nigra extract on human prostate cancer cells. Saudi Pharm. J. 2017, 25, 241–248
- 23. Dalkilic, S., Dalkilic, L.K., İnci, S., Korkmaz, İ., Kirbag, S. . Investigation of cytotoxic effect of black mulberry (Morus nigra L.) fruit, Indian Journal of Traditional Knowledge, 2021,20, 54-58.
- 24. Dalmagro AP, Camargo A, Severo Rodrigues AL, Zeni ALB. Involvement of PI3K/Akt/GSK-3β signaling pathway in the antidepressant-like and neuroprotective effects of Morus nigra and its major phenolic, syringic acid. Chem Biol Interact. 2019 Dec 1; 314:108843. doi: 10.1016/j.cbi.2019.108843.
- 25. Souza GR, Oliveira-Junior RG, Diniz TC, Branco A, Lima-Saraiva SRG, Guimarães AL, Oliveira AP, Pacheco AGM, Silva MG, Moraes-Filho MO, Costa MP, Pessoa CÓ, Almeida JRGS. Assessment of the antibacterial, cytotoxic and antioxidant activities of Morus nigra L. (Moraceae). Braz J Biol. 2018 May;78(2):248-254. doi: 10.1590/1519-6984.05316.
Öz
Abstract
Aim; Morus Nigra (Moraceae) is a fruit frequently consumed by humans. It is also used in traditional medicine to treat various diseases because it has antiallergic, anti-inflammatory, antibacterial and anticancer properties. It has been stated that Morus Nigra extract has effects on cell viability and some sources do not have this effect. Therefore, this study was carried out to investigate the effects of Morus Nigra extract on neurotoxicity, apoptosis and cell viability in Nb2a cell line
Method: Methanol extracts of Morus Nigra were applied to Nb2a cell line at different concentrations (1, 3, 10, 30, 100, 300 and 1000 μg/ml). The effects on the cells were evaluated using neurotoxicity screening test (NTT), apoptosis detection by annexin V method and cell viability/proliferation tests.
Results: It was found that methanol extracts of Morus Nigra had no significant effect on cell viability and apoptosis, but showed moderate neuroprotective effect on neurite elongation at concentrations of 100 and 300 µg/ml.
Conclusion: These findings indicate that although Morus Nigra is not thought to have anticancer properties, it has been shown that the phenolic compounds it contains may protect axons in the neuronal system due to their antioxidant effects.
Keywords: Morus Nigra, Cell culture, Nb2a, apoptosis, NTT
Anahtar Kelimeler
Kaynakça
- 1. Gundogdu M, Muradoglu F, Sensoy RIG et al Determination of fruit chemical properties of Morus nigra L., Morus alba L. and Morus rubra L. by HPLC. Sci Hortic 132:37–41. doi:10.1016/j.scienta.2011.09.035
- 2. Ozgen M., Serce S., Kaya C. Phytochemical and antioxidant properties of anthocyanin-rich Morus nigra and Morus rubra fruits. Scientia Horticulturae. 2009;119(3):275–279. doi: 10.1016/j.scienta.2008.08.007.
- 3. Souza G. R., Oliveira-Junior R. G., Diniz T. C., et al. Assessment of the antibacterial, cytotoxic and antioxidant activities of Morus nigra L. (Moraceae) Brazilian Journal of Biology. 2018;78(2):248–254. doi: 10.1590/1519-6984.05316.
- 4. Padilha M. M., Vilela F. C., Rocha C. Q., et al. Antiinflammatory properties of Morus nigra leaves. Phytotherapy Research. 2010; 24(10):1496–1500. doi: 10.1002/ptr.3134.]
- 5. Chen H., Pu J., Liu D., et al. Anti-inflammatory and antinociceptive properties of flavonoids from the fruits of black mulberry (Morus nigra L.) PLoS One. 2016;11(4): p. e0153080. doi: 10.1371/journal.pone.0153080.
- 6. Van Hung P. Phenolic Compounds of Cereals and Their Antioxidant Capacity. Critical Reviews in Food Science and Nutrition, 2016 56 (1): 25-35.
- 7. Cheatham, C.L.; Canipe, L.G., 3rd; Millsap, G.; Stegall, J.M.; Chai, S.C.; Sheppard, K.W.; Lila, M.A. Six-month intervention with wild blueberries improved speed of processing in mild cognitive decline: A double-blind, placebo-controlled, randomized clinical trial. Nutr. Neurosci. 2022, 1–15
- 8. Cheatham, C.L.; Nieman, D.C.; Neilson, A.P.; Lila, M.A. Enhancing the Cognitive Effects of Flavonoids with Physical Activity: Is There a Case for the Gut Microbiome? Front. Neurosci. 2022, 16, 833202.
- 9. Lau, F.C.; Shukitt-Hale, B.; Joseph, J.A. Nutritional intervention in brain aging: Reducing the effects of inflammation and oxidative stress. Subcell. Biochem. 2007, 42, 299–318.
- 10. Albarracin, S.L.; Stab, B.; Casas, Z.; Sutachan, J.J.; Samudio, I.; Gonzalez, J.; Gonzalo, L.; Capani, F.; Morales, L.; Barreto, G.E. Effects of natural antioxidants in neurodegenerative disease. Nutr. Neurosci. 2012, 15, 1–9.]
- 11. Akter, R.; Rahman, H.; Behl, T.; Chowdhury, M.A.R.; Manirujjaman, M.; Bulbul, I.J.; Elshenaw, S.E.; Tit, D.M.; Bungau, S. Prospective Role of Polyphenolic Compounds in the Treatment of Neurodegenerative Diseases. CNS Neurol. Disord. Drug Targets 2021, 20, 430–450.
- 12. Mattioli R, Francioso A, Mosca L, Silva P. Anthocyanins: A Comprehensive Review of Their Chemical Properties and Health Effects on Cardiovascular and Neurodegenerative Diseases. Molecules. 2020 Aug 21;25(17):3809. doi: 10.3390/molecules25173809.
- 13. Shahneh F.Z., Baradaran B., Majidi J., Babaloo Z. Echinophora platyloba DC (Apiaceae) crude extract induces apoptosis in human prostate adenocarcinoma cells (PC-3) Biomed. J. 2014; 37:298–304. doi: 10.4103/2319-4170.125653
- 14. Vural K., Seyrek O. The Neuroprotective Effect of Pioglitazone on NB2a Mouse Neuroblastoma Cell Culture. Kafkas Univ Vet Fak Derg, 25 (1): 1-8, 2019. DOI: 10.9775/kvfd.2018.19940
- 15. Tatlı G., Vural K., Darıverenli E. Nöropeptid Y'nin fare nöroblastoma (NB2A) hücre kültür dizinindeki nöroprotektif etkinliğinin araştırılması 2023 Tıpta Uzmanlık Tezi
- 16. Khalid N., Fawad S.A., Ahmed I. Antimicrobial activity, phytochemical profile and trace minerals of black mulberry (Morus nigra L.) fresh juice. Pak. J. Bot. 2011; 43:91–96
- 17. Ramos S Cancer chemoprevention and chemotherapy. Dietary polyphenols and signalling pathways. Mol. Nutr. Food, 2008 Res.52: 507-526.
- 18. Huang WU, Cai ZY and Zhang J Natural phenolic compounds from medicinal herbs and dietary plants: potential use for cancer prevention. Nutr. Cancer , 2010 62 (1): 1- 20.
- 19. Borbalan AMA, Zorro L, Gullen DA and Barroso CG Study of the polyphenol content of red and white grape varieties by liquid chromatographymass spectrometry and its retalionship to antioxidant power. J. Chrom. A, 2003 1012: 31-38
- 20. Çakıroğlu, E.; Uysal, T.; Çalıbaşı Koçal, G.; Aygenli, F.; Baran, G.; Baskın, Y. The role of Morus nigra extract and its active compounds as drug candidate on human colorectal adenocarcinoma cell line HT-29. Int. J. Clin. Oncol. Cancer Res. 2017, 2, 10–14
- 21. Qadir, M.I.; Ali, M.; Ibrahim, Z. Anticancer activity of Morus nigra leaves extract. Bangladesh J. Pharmacol. 2014, 9, 496–497
- 22. Turan, I.; Demir, S.; Kilinc, K.; Burnaz, N.A.; Yaman, S.O.; Akbulult, K.; Mentese, A.; Aliyazicioglu, Y.; Deger, O. Antiproliferative and apoptotic effect of Morus nigra extract on human prostate cancer cells. Saudi Pharm. J. 2017, 25, 241–248
- 23. Dalkilic, S., Dalkilic, L.K., İnci, S., Korkmaz, İ., Kirbag, S. . Investigation of cytotoxic effect of black mulberry (Morus nigra L.) fruit, Indian Journal of Traditional Knowledge, 2021,20, 54-58.
- 24. Dalmagro AP, Camargo A, Severo Rodrigues AL, Zeni ALB. Involvement of PI3K/Akt/GSK-3β signaling pathway in the antidepressant-like and neuroprotective effects of Morus nigra and its major phenolic, syringic acid. Chem Biol Interact. 2019 Dec 1; 314:108843. doi: 10.1016/j.cbi.2019.108843.
- 25. Souza GR, Oliveira-Junior RG, Diniz TC, Branco A, Lima-Saraiva SRG, Guimarães AL, Oliveira AP, Pacheco AGM, Silva MG, Moraes-Filho MO, Costa MP, Pessoa CÓ, Almeida JRGS. Assessment of the antibacterial, cytotoxic and antioxidant activities of Morus nigra L. (Moraceae). Braz J Biol. 2018 May;78(2):248-254. doi: 10.1590/1519-6984.05316.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Sinirbilim (Diğer) |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Yayımlanma Tarihi | 27 Haziran 2025 |
| Gönderilme Tarihi | 31 Ocak 2025 |
| Kabul Tarihi | 19 Haziran 2025 |
| Yayımlandığı Sayı | Yıl 2025 Cilt: 12 Sayı: 2 |
