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Sensitive Analysis of Epilepsy Drug, Phenobarbital, Based on Column Type Solid Phase Extraction and HPLC-DAD System

Yıl 2024, Cilt: 45 Sayı: 4, 763 - 768, 30.12.2024

Halil İbrahim Ulusoy , Seren Kilit , Ümmügülsüm Polat , Esra Durgun , Aslıhan Gürbüzer , Elif Tümay Özer , Bilgen Osman

https://doi.org/10.17776/csj.1572205

Öz

An analytical approach has been developed for the sensitive determination of Phenobarbital, which is used as an antiepileptic drug molecule. The analysis of this active molecule, which has a very limited study even for its direct determination in the literature review, is mostly done with highly complex device systems such as LC-MS. In analysis with conventional HPLC systems, the limit of detection cannot be in most cases lower than 1 µg mL-1 With this study, a separation and preconcentration method based on solid phase extraction (SPE) was developed for trace phenobarbital molecules, so that even very low concentrations could be monitored. In the proposed method, the target molecules were enriched with column type SPE method, and then their analysis were carried out by with the HPLC-DAD system. As an SPE sorbent, a polymeric material, poly(ethylene glycol dimethacrylate-N-methacryloyl-L-tryptophan methyl ester) [poly(EGDMA-MATrp)], was used in extraction experiments. Experimental variables such as pH of medium, type and amount of desorption solvent, electrolyte effect have been studied and optimized step by step. The linear working range under the optimized conditions were determined in the range of 10.00-400.00 ng mL-1 with the limit of detection as 3.57 ng mL-1. Quantitative results were obtained in recovery experiments with the help of model solutions including phenobarbital molecule

Anahtar Kelimeler

Phenobarbital Solid phase extraction HPLC Analytical method development

Kaynakça

  • [1] Vermeij T. A. C., Edelbroek P. M., Robust isocratic high performance liquid chromatographic method for simultaneous determination of seven antiepileptic drugs including lamotrigine, oxcarbazepine and zonisamide in serum after solid-phase extraction, Journal of Chromatography B., 857(1) (2007) 40-46.
  • [2] Velayati S., Saadati F., Shayani-Jam H., Shekari A., Valipour R., Yaftian M. R., Fabrication and evaluation of a molecularly imprinted polymer electrochemical nanosensor for the sensitive monitoring of phenobarbital in biological samples, Microchemical Journal, 174 (2022) 107063.
  • [3] Mobed A., Shirafkan M., Charsouei S., Sadeghzadeh J., Ahmadalipour A., Biosensors technology for anti-epileptic drugs, Clinica Chimica Acta, 533 (2022) 175-182.
  • [4] Lyseng-Williamson K. A., Levetiracetam: a review of its use in epilepsy, Drugs, 71 (2011) 489-514.
  • [5] A Spectrophotometric Method for the Determination of Nitrite and Nitrate, Eurasian J. Anal. Chem., (2017).
  • [6] Jacob S., Nair A. B., An updated overview on therapeutic drug monitoring of recent antiepileptic drugs, Drugs in R&D, 16 (2016) 303-316.
  • [7] Ventura S., Rodrigues M., Pousinho S., Falcão A., Alves G., Determination of lamotrigine in human plasma and saliva using microextraction by packed sorbent and high performance liquid chromatography–diode array detection: An innovative bioanalytical tool for therapeutic drug monitoring, Microchemical Journal, 130 (2017) 221-228.
  • [8] D’Urso A., Locatelli M., Tartaglia A., Molteni L., D’Ovidio C., Savini F., de Grazia U., Therapeutic drug monitoring of antiseizure medications using volumetric absorptive microsampling: where are we?, Pharmaceuticals, 14(7) (2021) 627.
  • [9] Sultan M., Simultaneous HPLC determination and validation of amphetamine, methamphetamine, caffeine, paracetamol and theophylline in illicit seized tablets, Int. J. Pharm. Pharm. Sci., 6(4) (2014) 294-298.
  • [10] Pena A., Pelantova N., Lino C. M., Silveira, M. I. N., & Solich, P., Validation of an analytical methodology for determination of oxytetracycline and tetracycline residues in honey by HPLC with fluorescence detection, Journal of Agricultural and Food Chemistry, 53(10) (2005) 3784-3788.
  • [11] Ulusoy H. İ., Yiğit İ. N., Polat Ü., Durgun E., Gürbüzer A., Ulusoy S., Simultaneously HPLC Analysis of B1, B9 and B12 Vitamins at Trace Levels via Cloud Point Extraction, Cumhuriyet Science Journal, 44(4) (2023) 716-722.
  • [12] Ulusoy H. I., Polat U., Ulusoy S., Use of newly synthetized magnetic Fe 3 O 4 nanoparticles modified with hexadecyl trimethyl ammonium bromide for the sensitive analysis of antidepressant drugs, duloxetine and vilazodone in wastewater and urine samples, RSC Advances, 13(29) (2023) 20125-20134.
  • [13] Ulusoy S., Erdoğan S., Karaslan M. G., Ateş B., Ulusoy H. İ., Erdemoğlu S., Optimization Of Extraction Parameters For Folic Acid And Antioxidant Compounds From An Edible Plant (Polygonum Cognatum Meissn) Using Pressurized Liquid Extraction (PLE) System, Cumhuriyet Science Journal, 39(4) (2018) 1069-1080.
  • [14] Karaca E., Ulusoy S., Morgül Ü., Ulusoy H. İ., Development of analytical method for sensitive determination of streptozotocin based on solid phase extraction, Cumhuriyet Science Journal, 41(4) (2020) 826-831.
  • [15] Demir Ö., Ulusoy H. İ., Özer E. T., Osman, B., Development of a new solid phase extraction method for sensitive determination of some carbamate pesticides in water using poly (EGDMA-MATrp) microbeads, Microchemical Journal, 158 (2020) 105317.
  • [16] Osman B., Özer E. T., Kara A., Yeşilova E., Beşirli N., Properties of magnetic microbeads in removing bisphenol-A from aqueous phase, Journal of Porous Materials, 22 (2015) 37-46.
  • [17] Methaneethorn J., Leelakanok N., Pharmacokinetic variability of phenobarbital: a systematic review of population pharmacokinetic analysis, European Journal of Clinical Pharmacology, 77 (2021)291-309.

Yıl 2024, Cilt: 45 Sayı: 4, 763 - 768, 30.12.2024

Halil İbrahim Ulusoy , Seren Kilit , Ümmügülsüm Polat , Esra Durgun , Aslıhan Gürbüzer , Elif Tümay Özer , Bilgen Osman

https://doi.org/10.17776/csj.1572205

Öz

Kaynakça

  • [1] Vermeij T. A. C., Edelbroek P. M., Robust isocratic high performance liquid chromatographic method for simultaneous determination of seven antiepileptic drugs including lamotrigine, oxcarbazepine and zonisamide in serum after solid-phase extraction, Journal of Chromatography B., 857(1) (2007) 40-46.
  • [2] Velayati S., Saadati F., Shayani-Jam H., Shekari A., Valipour R., Yaftian M. R., Fabrication and evaluation of a molecularly imprinted polymer electrochemical nanosensor for the sensitive monitoring of phenobarbital in biological samples, Microchemical Journal, 174 (2022) 107063.
  • [3] Mobed A., Shirafkan M., Charsouei S., Sadeghzadeh J., Ahmadalipour A., Biosensors technology for anti-epileptic drugs, Clinica Chimica Acta, 533 (2022) 175-182.
  • [4] Lyseng-Williamson K. A., Levetiracetam: a review of its use in epilepsy, Drugs, 71 (2011) 489-514.
  • [5] A Spectrophotometric Method for the Determination of Nitrite and Nitrate, Eurasian J. Anal. Chem., (2017).
  • [6] Jacob S., Nair A. B., An updated overview on therapeutic drug monitoring of recent antiepileptic drugs, Drugs in R&D, 16 (2016) 303-316.
  • [7] Ventura S., Rodrigues M., Pousinho S., Falcão A., Alves G., Determination of lamotrigine in human plasma and saliva using microextraction by packed sorbent and high performance liquid chromatography–diode array detection: An innovative bioanalytical tool for therapeutic drug monitoring, Microchemical Journal, 130 (2017) 221-228.
  • [8] D’Urso A., Locatelli M., Tartaglia A., Molteni L., D’Ovidio C., Savini F., de Grazia U., Therapeutic drug monitoring of antiseizure medications using volumetric absorptive microsampling: where are we?, Pharmaceuticals, 14(7) (2021) 627.
  • [9] Sultan M., Simultaneous HPLC determination and validation of amphetamine, methamphetamine, caffeine, paracetamol and theophylline in illicit seized tablets, Int. J. Pharm. Pharm. Sci., 6(4) (2014) 294-298.
  • [10] Pena A., Pelantova N., Lino C. M., Silveira, M. I. N., & Solich, P., Validation of an analytical methodology for determination of oxytetracycline and tetracycline residues in honey by HPLC with fluorescence detection, Journal of Agricultural and Food Chemistry, 53(10) (2005) 3784-3788.
  • [11] Ulusoy H. İ., Yiğit İ. N., Polat Ü., Durgun E., Gürbüzer A., Ulusoy S., Simultaneously HPLC Analysis of B1, B9 and B12 Vitamins at Trace Levels via Cloud Point Extraction, Cumhuriyet Science Journal, 44(4) (2023) 716-722.
  • [12] Ulusoy H. I., Polat U., Ulusoy S., Use of newly synthetized magnetic Fe 3 O 4 nanoparticles modified with hexadecyl trimethyl ammonium bromide for the sensitive analysis of antidepressant drugs, duloxetine and vilazodone in wastewater and urine samples, RSC Advances, 13(29) (2023) 20125-20134.
  • [13] Ulusoy S., Erdoğan S., Karaslan M. G., Ateş B., Ulusoy H. İ., Erdemoğlu S., Optimization Of Extraction Parameters For Folic Acid And Antioxidant Compounds From An Edible Plant (Polygonum Cognatum Meissn) Using Pressurized Liquid Extraction (PLE) System, Cumhuriyet Science Journal, 39(4) (2018) 1069-1080.
  • [14] Karaca E., Ulusoy S., Morgül Ü., Ulusoy H. İ., Development of analytical method for sensitive determination of streptozotocin based on solid phase extraction, Cumhuriyet Science Journal, 41(4) (2020) 826-831.
  • [15] Demir Ö., Ulusoy H. İ., Özer E. T., Osman, B., Development of a new solid phase extraction method for sensitive determination of some carbamate pesticides in water using poly (EGDMA-MATrp) microbeads, Microchemical Journal, 158 (2020) 105317.
  • [16] Osman B., Özer E. T., Kara A., Yeşilova E., Beşirli N., Properties of magnetic microbeads in removing bisphenol-A from aqueous phase, Journal of Porous Materials, 22 (2015) 37-46.
  • [17] Methaneethorn J., Leelakanok N., Pharmacokinetic variability of phenobarbital: a systematic review of population pharmacokinetic analysis, European Journal of Clinical Pharmacology, 77 (2021)291-309.
Toplam 17 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Eczacılıkta Analitik Kimya, Separasyon Bilimi
Bölüm Natural Sciences
Yazarlar

Halil İbrahim Ulusoy 0000-0002-8742-5145

Seren Kilit 0009-0003-4453-4215

Ümmügülsüm Polat 0000-0001-8393-2396

Esra Durgun 0009-0009-2613-4019

Aslıhan Gürbüzer 0000-0002-6753-2808

Elif Tümay Özer 0000-0002-5225-0146

Bilgen Osman 0000-0001-8406-149X

Yayımlanma Tarihi 30 Aralık 2024
Gönderilme Tarihi 23 Ekim 2024
Kabul Tarihi 19 Aralık 2024
Yayımlandığı Sayı Yıl 2024 Cilt: 45 Sayı: 4

Kaynak Göster

APA Ulusoy, H. İ., Kilit, S., Polat, Ü., Durgun, E., vd. (2024). Sensitive Analysis of Epilepsy Drug, Phenobarbital, Based on Column Type Solid Phase Extraction and HPLC-DAD System. Cumhuriyet Science Journal, 45(4), 763-768. https://doi.org/10.17776/csj.1572205
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