Araştırma Makalesi
Evaluating the Synergistic Effects of Oleuropein and Vitamin C on Head and Neck Cancer Cell Viability and Migration
Öz
Cancer's standard treatment is generally based on cytotoxic drugs, radiotherapy, chemotherapy, and surgery. One treatment type in the study is phytotherapy, which uses plant components to treat diseases or improve health. Oleuropein, obtained from olive leaves, is active in olive leaves and fruit. It affects health, such as skin protection, anti-aging, antiviral, and antioxidants. Recent studies have proven that it positively affects humans, including cancer prevention and antimicrobial properties. The other substance to be combined with oleuropein is vitamin C, which has an essential function in the body, inactivates carcinogenic substances taken into the body, and is a known antioxidant that forms a shield against cancer. We will investigate the therapeutic effects of combining oleuropein and vitamin C on head-neck cancer cells (Fadu). We conducted cell viability and scratch (migration) analyses on Fadu cells treated with oleuropein and/or vitamin C at different doses for 24 hours. The cell viability analyses showed that oleuropein and vitamin C treatments significantly reduced cell viability. At the same time, the combined applications of oleuropein and vitamin C also considerably reduced cell viability. In the cell migration test, we observed that oleuropein or vitamin C alone increased the migration while the combined applications significantly reduced it. The results indicate that applying oleuropein or vitamin C leads to decreased cell viability. However, the combined applications of oleuropein and vitamin C offer an effective treatment option by reducing cell viability and migration.
Anahtar Kelimeler
Kaynakça
- 1. Akao, Y., Fujii, E., Furutani, Y., & Tohyama, M. (2007). Cancer prevention and therapy with oleuropein. Journal of Natural Medicines, 61(1), 30–35.
- 2. Alexander, W., & Vidyasagar, D. (1993). Cellular mechanisms of cancer progression. Cancer Research Journal, 50(12), 1503–1512.
- 3. Cárcamo, J. M., Pedraza, A., Bórquez-Ojeda, O., Zhang, B., Sanchez, R., & Golde, D. W. (2002). Vitamin C is a kinaseinhibitor: dehydroascorbic acid inhibits IκBα kinase β. Molecular Cell Biology, 22(11), 3873–3881. https://doi.org/10.1128/MCB.22.11.3873-3881.2002
- 4. Du, Q., Barhoumi, R., & Milner, J. A. (2010). Oleuropein and cancer: suppression of prostate cancer cell growth by blocking NF-κB activation. Journal of Nutritional Biochemistry, 21(8), 595–601. https://doi.org/10.1016/j.jnutbio.2009.03.011
- 5. Ertan, S. (1967). Kanserin biyolojik mekanizmaları. Türk Onkoloji Dergisi, 5(2), 45-52.
- 6. Forastiere, A. A., Johnson, D. E., Patel, S. G., & Wenig, B. M. (2023). Advances in head and neck cancer treatment. The New England Journal of Medicine, 388(1), 50–65.
- 7. Gikas, E., Triantafyllidis, A., & Tsarbopoulos, A. (2007). Antioxidant properties of oleuropein. Phytomedicine, 14(6), 423–430.
- 8. Han, J., Talorete, T. P. N., Yamada, P., Isoda, H. (2016). Anti-proliferative and anti-invasive effects of oleuropein on breast cancer cells. Journal of Natural Medicines, 70(2), 354–362. https://doi.org/10.1007/s11418-016-0975-0
- 9. İbrahim, M. (2020). Modern cancer diagnostics and therapeutics. Medical Oncology Journal, 40(3), 245–260.
- 10. Impellizzeri, D., Esposito, E., Mazzon, E., Paterniti, I., Di Paola, R., & Cuzzocrea, S. (2011). Oleuropein aglycone protects against ischemic brain injury via autophagy regulation. Frontiers in Bioscience (Elite Edition), 3, 1079–1091. https://doi.org/10.2741/e326
- 11. Jacob, R. A., & Sotoudeh, G. (2002). Vitamin C function and status in chronic disease. *Nutrition in Clinical Care*, 5(2), 66-74.
- 12. Koyun, M. T., Sirin, S., Aslim, B., Taner, G., & Dolanbay, S. N. (2022). Characterization of prodigiosin pigment by Serratia marcescens and the evaluation of its bioactivities. Toxicology in vitro, 82, 105368.
- 13. Lee, C. J., Tsai, C. H., Pan, T. M., & Tsai, T. Y. (2013). Oleuropein inhibits breast cancer cell growth and metastasis via suppression of the epithelial-mesenchymal transition. Nutrition and Cancer, 65(6), 847–856. https://doi.org/10.1080/01635581.2013.805986
- 14. Lee, J. H., Lee, H. J., & Lee, S. H. (2013). Oleuropein inhibits the migration and invasion of human colorectal carcinoma cells. *Journal of Medicinal Food*, 16(11), 1040-1046.
- 15. Li, Y., Li, S., & Li, H. (2012). Oleuropein inhibits proliferation and induces apoptosis in human prostate cancer cells. *International Journal of Oncology*, 41(5), 1675-1682.
- 16. Mandl, J., Szarka, A., & Bánhegyi, G. (2009). Vitamin C: update on physiology and phamacology. British Journal of Pharmacology, 157(7), 1097–1110. https://doi.org/10.1111/j.1476-5381.2009.00282.x
- 17. Morpa, H. (2005). Vitamin C and its role in human health. Nutritional Sciences, 25(4), 100-115.
- 18. Omar, S. H. (2010). Oleuropein in preventing cancer. Cancer Prevention Research, 3(8), 1035-1040.
- 19. Öztürk, E., Çalık, F., & Ulusoy, D. (2022). Investigation of cytotoxic and genotoxic effects of olive leaf extract on colon cancer cells and normal cell lines. *Eurasian Mol Biochem Sci*, 1(2), 25-30.
- 20. Powell, J., Smith, A., Lee, K., Patel, R., Johnson, M., Chen, L., & Davis, P. (2011). Free radicals and cancer therapy. Cancer Treatment Reviews, 37(4), 321–329.
- 21. Preedy, V. R., & Watson, R. R. (2020). The role of antioxidants in disease prevention. Springer Science & Business Media.
- 22. Shen, L., Wang, H., Zhang, X., Li, Y., Chen, J., Liu, Z., & Zhao, Q. (2017). Natural products as potential cancer therapeutics. Oncotarget, 8(12), 20055–20070
- 23. Sözmen, E. Y. (2002). C vitamininin biyokimyasal rolleri. Türk Biyokimya Dergisi, 27(3), 65-72.
- 24. Taşkın, A. (2023). Bortezomib ve C vitamini Kombinasyonunun HL-60 Akut Promyelositik Lösemi Hücrelerindeki Etkisinin Değerlendirilmesi. Harran Üniversitesi Tıp Fakültesi Dergisi, 20(2), 418-424.
- 25. Waridel, P., Smith, J., & Johnson, L. (1997). Biochemical properties of oleuropein. Journal of Biochemistry, 122(6), 987–995.
Yıl 2025,
Cilt: 4 Sayı: 1, 11 - 17, 13.07.2025
Öz
Kaynakça
- 1. Akao, Y., Fujii, E., Furutani, Y., & Tohyama, M. (2007). Cancer prevention and therapy with oleuropein. Journal of Natural Medicines, 61(1), 30–35.
- 2. Alexander, W., & Vidyasagar, D. (1993). Cellular mechanisms of cancer progression. Cancer Research Journal, 50(12), 1503–1512.
- 3. Cárcamo, J. M., Pedraza, A., Bórquez-Ojeda, O., Zhang, B., Sanchez, R., & Golde, D. W. (2002). Vitamin C is a kinaseinhibitor: dehydroascorbic acid inhibits IκBα kinase β. Molecular Cell Biology, 22(11), 3873–3881. https://doi.org/10.1128/MCB.22.11.3873-3881.2002
- 4. Du, Q., Barhoumi, R., & Milner, J. A. (2010). Oleuropein and cancer: suppression of prostate cancer cell growth by blocking NF-κB activation. Journal of Nutritional Biochemistry, 21(8), 595–601. https://doi.org/10.1016/j.jnutbio.2009.03.011
- 5. Ertan, S. (1967). Kanserin biyolojik mekanizmaları. Türk Onkoloji Dergisi, 5(2), 45-52.
- 6. Forastiere, A. A., Johnson, D. E., Patel, S. G., & Wenig, B. M. (2023). Advances in head and neck cancer treatment. The New England Journal of Medicine, 388(1), 50–65.
- 7. Gikas, E., Triantafyllidis, A., & Tsarbopoulos, A. (2007). Antioxidant properties of oleuropein. Phytomedicine, 14(6), 423–430.
- 8. Han, J., Talorete, T. P. N., Yamada, P., Isoda, H. (2016). Anti-proliferative and anti-invasive effects of oleuropein on breast cancer cells. Journal of Natural Medicines, 70(2), 354–362. https://doi.org/10.1007/s11418-016-0975-0
- 9. İbrahim, M. (2020). Modern cancer diagnostics and therapeutics. Medical Oncology Journal, 40(3), 245–260.
- 10. Impellizzeri, D., Esposito, E., Mazzon, E., Paterniti, I., Di Paola, R., & Cuzzocrea, S. (2011). Oleuropein aglycone protects against ischemic brain injury via autophagy regulation. Frontiers in Bioscience (Elite Edition), 3, 1079–1091. https://doi.org/10.2741/e326
- 11. Jacob, R. A., & Sotoudeh, G. (2002). Vitamin C function and status in chronic disease. *Nutrition in Clinical Care*, 5(2), 66-74.
- 12. Koyun, M. T., Sirin, S., Aslim, B., Taner, G., & Dolanbay, S. N. (2022). Characterization of prodigiosin pigment by Serratia marcescens and the evaluation of its bioactivities. Toxicology in vitro, 82, 105368.
- 13. Lee, C. J., Tsai, C. H., Pan, T. M., & Tsai, T. Y. (2013). Oleuropein inhibits breast cancer cell growth and metastasis via suppression of the epithelial-mesenchymal transition. Nutrition and Cancer, 65(6), 847–856. https://doi.org/10.1080/01635581.2013.805986
- 14. Lee, J. H., Lee, H. J., & Lee, S. H. (2013). Oleuropein inhibits the migration and invasion of human colorectal carcinoma cells. *Journal of Medicinal Food*, 16(11), 1040-1046.
- 15. Li, Y., Li, S., & Li, H. (2012). Oleuropein inhibits proliferation and induces apoptosis in human prostate cancer cells. *International Journal of Oncology*, 41(5), 1675-1682.
- 16. Mandl, J., Szarka, A., & Bánhegyi, G. (2009). Vitamin C: update on physiology and phamacology. British Journal of Pharmacology, 157(7), 1097–1110. https://doi.org/10.1111/j.1476-5381.2009.00282.x
- 17. Morpa, H. (2005). Vitamin C and its role in human health. Nutritional Sciences, 25(4), 100-115.
- 18. Omar, S. H. (2010). Oleuropein in preventing cancer. Cancer Prevention Research, 3(8), 1035-1040.
- 19. Öztürk, E., Çalık, F., & Ulusoy, D. (2022). Investigation of cytotoxic and genotoxic effects of olive leaf extract on colon cancer cells and normal cell lines. *Eurasian Mol Biochem Sci*, 1(2), 25-30.
- 20. Powell, J., Smith, A., Lee, K., Patel, R., Johnson, M., Chen, L., & Davis, P. (2011). Free radicals and cancer therapy. Cancer Treatment Reviews, 37(4), 321–329.
- 21. Preedy, V. R., & Watson, R. R. (2020). The role of antioxidants in disease prevention. Springer Science & Business Media.
- 22. Shen, L., Wang, H., Zhang, X., Li, Y., Chen, J., Liu, Z., & Zhao, Q. (2017). Natural products as potential cancer therapeutics. Oncotarget, 8(12), 20055–20070
- 23. Sözmen, E. Y. (2002). C vitamininin biyokimyasal rolleri. Türk Biyokimya Dergisi, 27(3), 65-72.
- 24. Taşkın, A. (2023). Bortezomib ve C vitamini Kombinasyonunun HL-60 Akut Promyelositik Lösemi Hücrelerindeki Etkisinin Değerlendirilmesi. Harran Üniversitesi Tıp Fakültesi Dergisi, 20(2), 418-424.
- 25. Waridel, P., Smith, J., & Johnson, L. (1997). Biochemical properties of oleuropein. Journal of Biochemistry, 122(6), 987–995.
Toplam 25 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Bitki Hücresi ve Moleküler Biyoloji, Biyokimya ve Hücre Biyolojisi (Diğer) |
| Bölüm | Research Article[En] |
| Yazarlar | |
| Erken Görünüm Tarihi | 21 Temmuz 2025 |
| Yayımlanma Tarihi | 13 Temmuz 2025 |
| Gönderilme Tarihi | 27 Mart 2025 |
| Kabul Tarihi | 1 Temmuz 2025 |
| Yayımlandığı Sayı | Yıl 2025 Cilt: 4 Sayı: 1 |
