Araştırma Makalesi
Ricinus Communis'in İnsan Diş Eti Fibroblast Hücrelerinin Canlılığı Üzerine Etkisi: Bir In Vitro Çalışma
Öz
Amaç: Bu çalışmanın amacı, Ricinus communis ekstresinin insan diş eti fibroblastlarında (iDEF) hücre canlılığı üzerindeki potansiyel etkilerini değerlendirmektir.
Gereç ve Yöntemler: Ricinus communis tohumlarından ultrasonik yöntem ve metanol çözücüsü kullanılarak ekstre elde edilmiştir. Hazırlanan ekstre 5 µg/ml, 10 µg/ml ve 15 µg/ml olacak şekilde üç farklı konsantrasyonlara seyreltildi. iDEF hücrelerine 24 saat süreyle Ricinus communis ekstreleri uygulandı. Canlılık değerlendirmesi için 3-(4,5-dimetiltiyazol-2-il)-2-5-difeniltetrazolyum bromür (MTT) analizi uygulandı. Verilerin istatistiksel analizleri SPSS paket programı kullanılarak değerlendirildi. İstatistiksel anlamlılık p<0.05 olarak kabul edildi.
Bulgular: Ricinus communis’in uygulandığı tüm konsantrasyonlarda kontrol grubuna kıyasla istatistiksel olarak anlamlı fark tespit edilmedi.
Sonuç: Bu bulgular, Ricinus communis ekstresinin iDEF hücrelerinde zararlı etkiler yaratmadığını, ancak hücre canlılığı üzerinde belirgin bir olumlu etki potansiyeline sahip olmadığını göstermektedir. Dolayısıyla, Ricinus communis ekstresinin yara iyileşmesi üzerindeki potansiyel etkilerini daha kapsamlı şekilde değerlendirebilmek için ek araştırmalar gerekmektedir.
Anahtar Kelimeler
Kaynakça
- Reference1. Abbas M, Ali A, Arshad M, Atta A, Mehmood Z, Tahir IM, et al. Mutagenicity, cytotoxic and antioxidant activities of Ricinus communis different parts. Chemistry Central Journal. 2018;12(1):3.
- Reference2. Ahangar P, Mills SJ, Smith LE, Gronthos S, Cowin AJ. Human gingival fibroblast secretome accelerates wound healing through anti-inflammatory and pro-angiogenic mechanisms. NPJ Regen Med. 2020;5(1):24.
- Reference3. AL Khafaji D, AL-Mukhtar E, Ghaleb R. Effect of Ricinus Communis Aqueous Leaf Extract on the Healing of Wound. An in Vitro Study in the Cell Line. Journal of Pharmaceutical Negative Results. 2022;13(4):395-402.
- Reference4. Alavi SH, Rezvani G, Esfahani MN, Nobakht Lahrood F. Periodontal Ligament Fibroblast Cell Viability Following Treatment with Different Concentrations of Green Tea, Aloe Vera and a Mixture of their Extracts. Front Dent. 2022;19:40.
- Reference5. Bartold PM, Walsh LJ, Narayanan AS. Molecular and cell biology of the gingiva. Periodontol 2000. 2000;24:28-55.
- Reference6. Bigi MF, Torkomian VL, de Groote ST, Hebling MJ, Bueno OC, Pagnocca FC, et al. Activity of Ricinus communis (Euphorbiaceae) and ricinine against the leaf-cutting ant Atta sexdens rubropilosa (Hymenoptera: Formicidae) and the symbiotic fungus Leucoagaricus gongylophorus. Pest Manag Sci. 2004;60(9):933-8.
- Reference7. Camargo SE, Camargo CH, Hiller KA, Rode SM, Schweikl H, Schmalz G. Cytotoxicity and genotoxicity of pulp capping materials in two cell lines. Int Endod J. 2009;42(3):227-37.
- Reference8. Clark RAF. Overview and General Considerations of Wound Repair. In: Clark RAF, Henson PM, editors. The Molecular and Cellular Biology of Wound Repair. Boston, MA: Springer US; 1988. p. 3-33.
- Reference9. Dale BA. Periodontal epithelium: a newly recognized role in health and disease. Periodontol 2000. 2002;30:70-8.
- Reference10. de Morais JPP, Pacheco IKC, Filho A, Ferreira DCL, Viana FJC, da Silva Reis F, et al. Polyurethane derived from castor oil monoacylglyceride (Ricinus communis) for bone defects reconstruction: characterization and in vivo testing. J Mater Sci Mater Med. 2021;32(4):39.
- Reference11. DesJardins-Park HE, Foster DS, Longaker MT. Fibroblasts and wound healing: an update. Regen Med. 2018;13(5):491-5.
- Reference12. Elkousy RH, Said ZNA, Abd El-Baseer MA, Abu El Wafa SA. Antiviral Activity of Castor Oil Plant (Ricinus communis) Leaf Extracts. Journal of ethnopharmacology. 2021:113878.
- Reference13. Fiorellini JP, Kim D, Chang Y. Anatomy, Structure, and Function of the Periodontium. In: Newman MG, Takei HH, Klokkevold PR, Carranza FA, editors. Carranza's Clinical Periodontology. 13th ed. Philadelphia: Elsevier; 2019. p. 19-49.
- Reference14. Ghnimi W, Dicko A, Khouja ML, el Ferchichi H. Larvicidal activity, phytochemical composition, and antioxidant properties of different parts of five populations of Ricinus communis L. Industrial Crops and Products. 2014;56:43–51.
- Reference15. Ilavarasan R, Mallika M, Venkataraman S. Anti-inflammatory and free radical scavenging activity of Ricinus communis root extract. J Ethnopharmacol. 2006;103(3):478-80.
- Reference16. Kuete V, Karaosmanoğlu O, Sivas H. Anticancer Activities of African Medicinal Spices and Vegetables. In: Kuete V, editor. Medicinal Spices and Vegetables from Africa: Academic Press; 2017. p. 271-97.
- Reference17. Kumar P, Nagarajan A, Uchil PD. Analysis of Cell Viability by the MTT Assay. Cold Spring Harb Protoc. 2018;2018(6).
- Reference18. Laureano Filho JR, Castelo Branco Bde L, Andrade ES, Barbosa JR. Histological comparison of demineralized bone matrix and the Ricinus communis polymer on bone regeneration. Braz J Otorhinolaryngol. 2007;73(2):186-92.
- Reference19. Leite FRM, Ramalho LTdO. Bone regeneration after demineralized bone matrix and castor oil (Ricinus communis) polyurethane implantation. Journal of Applied Oral Science. 2008;16:122-6.
- Reference20. Lindhe J, Karring T, Araújo M. The Anatomy of Periodontal Tissues. In: Lindhe J, Lang NP, Karring T, editors. Clinical Periodontology and Implant Dentistry. 1. 5th ed. UK: Blackwell; 2008. p. 3-48.
- Reference21. Mohammed NI, Albozachri JMK. Use of Iraqi Castor (Ricinus Communis) Leaf Extract as AntiInflammatory in Treatment of Skin Wounds in Rabbits. Medico-Legal Update. 2020;20:1321-5.
- Reference22. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65(1-2):55-63.
- Reference23. Nabavizadeh M, Abbaszadegan A, Khodabakhsi A, Ahzan S, Mehrabani D. Efficiency of Castor Oil as a Storage Medium for Avulsed Teeth in Maintaining the Viability of Periodontal Ligament Cells. J Dent (Shiraz). 2018;19(1):28-33.
- Reference24. Nanci A, Bosshardt DD. Structures of periodontal tissues in health and disease. Periodontol 2000. 2006;40:11-28.
- Reference25. Naz R, Bano A. Antimicrobial potential of Ricinus communis leaf extracts in different solvents against pathogenic bacterial and fungal strains. Asian Pac J Trop Biomed. 2012;2(12):944-7.
- Reference26. Nemudzivhadi V, Masoko P. In Vitro Assessment of Cytotoxicity, Antioxidant, and Anti-Inflammatory Activities of Ricinus communis (Euphorbiaceae) Leaf Extracts. Evid Based Complement Alternat Med. 2014;2014:625961.
- Reference27. Osorio-Paz I, Valle-Jiménez X, Brunauer R, Alavez S. Vanillic Acid Improves Stress Resistance and Substantially Extends Life Span in Caenorhabditis elegans. J Gerontol A Biol Sci Med Sci. 2023;78(7):1100-7.
- Reference28. Ozen J, Beydemir B, Serdar M, Ural A, Soysal Y. In vitro IL-1β release from gingival fibroblasts in response to pure metals, dental alloys and ceramic. Journal of oral rehabilitation. 2005;32:511-7.
- Reference29. Ribeiro PR, de Castro RD, Fernandez LG. Chemical constituents of the oilseed crop Ricinus communis and their pharmacological activities: A review. Industrial Crops and Products. 2016;91:358-76.
- Reference30. Scarpa A, Guerci A. Various uses of the castor oil plant (Ricinus communis L.). A review. J Ethnopharmacol. 1982;5(2):117-37.
- Reference31. Silva GO, Cavalcanti BN, Oliveira TR, Bin CV, Camargo SE, Camargo CH. Cytotoxicity and genotoxicity of natural resin-based experimental endodontic sealers. Clin Oral Investig. 2016;20(4):815-9.
- Reference32. Singh PP, Ambika, Chauhan SMS. Activity guided isolation of antioxidants from the leaves of Ricinus communis L. Food Chemistry. 2009;114(3):1069-72.
- Reference33. Singh V, Sharma DS, Dhar KL, Kalia AN. Activity guided isolation of anti-inflammatory compound/fraction from root of Ricinus communis Linn. International Journal of PharmTech Research. 2013;5:1142-9.
- Reference34. Sutherland BA, Rahman RM, Appleton I. Mechanisms of action of green tea catechins, with a focus on ischemia-induced neurodegeneration. J Nutr Biochem. 2006;17(5):291-306.
- Reference35. Suurbaar J, Mosobil R, Donkor A-M. Antibacterial and antifungal activities and phytochemical profile of leaf extract from different extractants of Ricinus communis against selected pathogens. BMC Research Notes. 2017;10:660.
- Reference36. Talebi Ardakani MR, Hajizadeh F, Yadegari Z. Comparison of Attachment and Proliferation of Human Gingival Fibroblasts on Different Collagen Membranes. Ann Maxillofac Surg. 2018;8(2):218-23.
- Reference37. Tomokiyo A, Wada N, Maeda H. Periodontal Ligament Stem Cells: Regenerative Potency in Periodontium. Stem Cells Dev. 2019;28(15):974-85.
- Reference38. Verma UP, Gupta A, Yadav RK, Tiwari R, Sharma R, Balapure AK. Cytotoxicity of chlorhexidine and neem extract on cultured human gingival fibroblasts through fluorescence-activated cell sorting analysis : An in-vitro study. Eur J Dent. 2018;12(3):344-9.
- Reference39. Ziaei A, Sahranavard S, Gharagozlou MJ, Faizi M. Preliminary investigation of the effects of topical mixture of Lawsonia inermis L. and Ricinus communis L. leaves extract in treatment of osteoarthritis using MIA model in rats. Daru. 2016;24(1):12.
Yıl 2025,
Cilt: 9 Sayı: 1, 33 - 38, 30.04.2025
Öz
Objective: The aim of this study is to evaluate the potential effects of Ricinus communis extract on cell viability in human gingival fibroblast cells (iDEF).
Materials and Methods: Ricinus communis seeds were used to prepare the extract using ultrasonic extraction and methanol solvent. The prepared extract was diluted to concentrations of 5 µg/ml, 10 µg/ml, and 15 µg/ml. iDEF cells were treated with Ricinus communis extracts for 24 hours. Cell viability was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The data were analyzed statistically using the SPSS software, and statistical significance was set at p<0.05.
Results: No statistically significant differences were observed between the Ricinus communis treated groups and the control group at all concentrations.
Conclusion: These findings suggest that Ricinus communis extract did not have harmful effects on iDEF cells, but did not show a significant positive effect on cell viability. Therefore, further research is required to comprehensively assess the potential effects of Ricinus communis extract on wound healing.
Anahtar Kelimeler
Kaynakça
- Reference1. Abbas M, Ali A, Arshad M, Atta A, Mehmood Z, Tahir IM, et al. Mutagenicity, cytotoxic and antioxidant activities of Ricinus communis different parts. Chemistry Central Journal. 2018;12(1):3.
- Reference2. Ahangar P, Mills SJ, Smith LE, Gronthos S, Cowin AJ. Human gingival fibroblast secretome accelerates wound healing through anti-inflammatory and pro-angiogenic mechanisms. NPJ Regen Med. 2020;5(1):24.
- Reference3. AL Khafaji D, AL-Mukhtar E, Ghaleb R. Effect of Ricinus Communis Aqueous Leaf Extract on the Healing of Wound. An in Vitro Study in the Cell Line. Journal of Pharmaceutical Negative Results. 2022;13(4):395-402.
- Reference4. Alavi SH, Rezvani G, Esfahani MN, Nobakht Lahrood F. Periodontal Ligament Fibroblast Cell Viability Following Treatment with Different Concentrations of Green Tea, Aloe Vera and a Mixture of their Extracts. Front Dent. 2022;19:40.
- Reference5. Bartold PM, Walsh LJ, Narayanan AS. Molecular and cell biology of the gingiva. Periodontol 2000. 2000;24:28-55.
- Reference6. Bigi MF, Torkomian VL, de Groote ST, Hebling MJ, Bueno OC, Pagnocca FC, et al. Activity of Ricinus communis (Euphorbiaceae) and ricinine against the leaf-cutting ant Atta sexdens rubropilosa (Hymenoptera: Formicidae) and the symbiotic fungus Leucoagaricus gongylophorus. Pest Manag Sci. 2004;60(9):933-8.
- Reference7. Camargo SE, Camargo CH, Hiller KA, Rode SM, Schweikl H, Schmalz G. Cytotoxicity and genotoxicity of pulp capping materials in two cell lines. Int Endod J. 2009;42(3):227-37.
- Reference8. Clark RAF. Overview and General Considerations of Wound Repair. In: Clark RAF, Henson PM, editors. The Molecular and Cellular Biology of Wound Repair. Boston, MA: Springer US; 1988. p. 3-33.
- Reference9. Dale BA. Periodontal epithelium: a newly recognized role in health and disease. Periodontol 2000. 2002;30:70-8.
- Reference10. de Morais JPP, Pacheco IKC, Filho A, Ferreira DCL, Viana FJC, da Silva Reis F, et al. Polyurethane derived from castor oil monoacylglyceride (Ricinus communis) for bone defects reconstruction: characterization and in vivo testing. J Mater Sci Mater Med. 2021;32(4):39.
- Reference11. DesJardins-Park HE, Foster DS, Longaker MT. Fibroblasts and wound healing: an update. Regen Med. 2018;13(5):491-5.
- Reference12. Elkousy RH, Said ZNA, Abd El-Baseer MA, Abu El Wafa SA. Antiviral Activity of Castor Oil Plant (Ricinus communis) Leaf Extracts. Journal of ethnopharmacology. 2021:113878.
- Reference13. Fiorellini JP, Kim D, Chang Y. Anatomy, Structure, and Function of the Periodontium. In: Newman MG, Takei HH, Klokkevold PR, Carranza FA, editors. Carranza's Clinical Periodontology. 13th ed. Philadelphia: Elsevier; 2019. p. 19-49.
- Reference14. Ghnimi W, Dicko A, Khouja ML, el Ferchichi H. Larvicidal activity, phytochemical composition, and antioxidant properties of different parts of five populations of Ricinus communis L. Industrial Crops and Products. 2014;56:43–51.
- Reference15. Ilavarasan R, Mallika M, Venkataraman S. Anti-inflammatory and free radical scavenging activity of Ricinus communis root extract. J Ethnopharmacol. 2006;103(3):478-80.
- Reference16. Kuete V, Karaosmanoğlu O, Sivas H. Anticancer Activities of African Medicinal Spices and Vegetables. In: Kuete V, editor. Medicinal Spices and Vegetables from Africa: Academic Press; 2017. p. 271-97.
- Reference17. Kumar P, Nagarajan A, Uchil PD. Analysis of Cell Viability by the MTT Assay. Cold Spring Harb Protoc. 2018;2018(6).
- Reference18. Laureano Filho JR, Castelo Branco Bde L, Andrade ES, Barbosa JR. Histological comparison of demineralized bone matrix and the Ricinus communis polymer on bone regeneration. Braz J Otorhinolaryngol. 2007;73(2):186-92.
- Reference19. Leite FRM, Ramalho LTdO. Bone regeneration after demineralized bone matrix and castor oil (Ricinus communis) polyurethane implantation. Journal of Applied Oral Science. 2008;16:122-6.
- Reference20. Lindhe J, Karring T, Araújo M. The Anatomy of Periodontal Tissues. In: Lindhe J, Lang NP, Karring T, editors. Clinical Periodontology and Implant Dentistry. 1. 5th ed. UK: Blackwell; 2008. p. 3-48.
- Reference21. Mohammed NI, Albozachri JMK. Use of Iraqi Castor (Ricinus Communis) Leaf Extract as AntiInflammatory in Treatment of Skin Wounds in Rabbits. Medico-Legal Update. 2020;20:1321-5.
- Reference22. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65(1-2):55-63.
- Reference23. Nabavizadeh M, Abbaszadegan A, Khodabakhsi A, Ahzan S, Mehrabani D. Efficiency of Castor Oil as a Storage Medium for Avulsed Teeth in Maintaining the Viability of Periodontal Ligament Cells. J Dent (Shiraz). 2018;19(1):28-33.
- Reference24. Nanci A, Bosshardt DD. Structures of periodontal tissues in health and disease. Periodontol 2000. 2006;40:11-28.
- Reference25. Naz R, Bano A. Antimicrobial potential of Ricinus communis leaf extracts in different solvents against pathogenic bacterial and fungal strains. Asian Pac J Trop Biomed. 2012;2(12):944-7.
- Reference26. Nemudzivhadi V, Masoko P. In Vitro Assessment of Cytotoxicity, Antioxidant, and Anti-Inflammatory Activities of Ricinus communis (Euphorbiaceae) Leaf Extracts. Evid Based Complement Alternat Med. 2014;2014:625961.
- Reference27. Osorio-Paz I, Valle-Jiménez X, Brunauer R, Alavez S. Vanillic Acid Improves Stress Resistance and Substantially Extends Life Span in Caenorhabditis elegans. J Gerontol A Biol Sci Med Sci. 2023;78(7):1100-7.
- Reference28. Ozen J, Beydemir B, Serdar M, Ural A, Soysal Y. In vitro IL-1β release from gingival fibroblasts in response to pure metals, dental alloys and ceramic. Journal of oral rehabilitation. 2005;32:511-7.
- Reference29. Ribeiro PR, de Castro RD, Fernandez LG. Chemical constituents of the oilseed crop Ricinus communis and their pharmacological activities: A review. Industrial Crops and Products. 2016;91:358-76.
- Reference30. Scarpa A, Guerci A. Various uses of the castor oil plant (Ricinus communis L.). A review. J Ethnopharmacol. 1982;5(2):117-37.
- Reference31. Silva GO, Cavalcanti BN, Oliveira TR, Bin CV, Camargo SE, Camargo CH. Cytotoxicity and genotoxicity of natural resin-based experimental endodontic sealers. Clin Oral Investig. 2016;20(4):815-9.
- Reference32. Singh PP, Ambika, Chauhan SMS. Activity guided isolation of antioxidants from the leaves of Ricinus communis L. Food Chemistry. 2009;114(3):1069-72.
- Reference33. Singh V, Sharma DS, Dhar KL, Kalia AN. Activity guided isolation of anti-inflammatory compound/fraction from root of Ricinus communis Linn. International Journal of PharmTech Research. 2013;5:1142-9.
- Reference34. Sutherland BA, Rahman RM, Appleton I. Mechanisms of action of green tea catechins, with a focus on ischemia-induced neurodegeneration. J Nutr Biochem. 2006;17(5):291-306.
- Reference35. Suurbaar J, Mosobil R, Donkor A-M. Antibacterial and antifungal activities and phytochemical profile of leaf extract from different extractants of Ricinus communis against selected pathogens. BMC Research Notes. 2017;10:660.
- Reference36. Talebi Ardakani MR, Hajizadeh F, Yadegari Z. Comparison of Attachment and Proliferation of Human Gingival Fibroblasts on Different Collagen Membranes. Ann Maxillofac Surg. 2018;8(2):218-23.
- Reference37. Tomokiyo A, Wada N, Maeda H. Periodontal Ligament Stem Cells: Regenerative Potency in Periodontium. Stem Cells Dev. 2019;28(15):974-85.
- Reference38. Verma UP, Gupta A, Yadav RK, Tiwari R, Sharma R, Balapure AK. Cytotoxicity of chlorhexidine and neem extract on cultured human gingival fibroblasts through fluorescence-activated cell sorting analysis : An in-vitro study. Eur J Dent. 2018;12(3):344-9.
- Reference39. Ziaei A, Sahranavard S, Gharagozlou MJ, Faizi M. Preliminary investigation of the effects of topical mixture of Lawsonia inermis L. and Ricinus communis L. leaves extract in treatment of osteoarthritis using MIA model in rats. Daru. 2016;24(1):12.
Toplam 39 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Periodontoloji |
| Bölüm | Özgün Araştırmalar |
| Yazarlar | |
| Yayımlanma Tarihi | 30 Nisan 2025 |
| Gönderilme Tarihi | 20 Şubat 2025 |
| Kabul Tarihi | 21 Mart 2025 |
| Yayımlandığı Sayı | Yıl 2025 Cilt: 9 Sayı: 1 |
