Öz
This study investigated plant growth-promoting characteristics of 389 candidate bacterial isolates collected during surveys conducted in 2017 by using nitrogen fixation, phosphorus reduction, and antagonistic activities tests. Six candidate bacterial isolates were identified as a result of the tests. The identification of these species was carried out using the MALDI-TOF MS technique, which identified species as Enterobacter cloacae, E. aerogenes, Bacillus cereus, Microbacterium testaceum, Pseudomonas putida, and Acinetobacter calcoaceticus. Pot and field trials were conducted using different combinations of these isolates. All isolates were used in the pot experiment, whereas isolates with the highest antagonistic activity and their combinations were used in the field trials. The pot trials were conducted using a randomized block design with five replications, while the field trials were conducted using a randomized complete block design with ten replications. The effects of these isolates on the yield of healthy pepper plants were investigated. Data relating to plant height, root length, stem diameter, number of fruits, plant fresh and dry weight, and root fresh and dry weight were collected. Results of the pot experiment revealed that combinations of B. cereus + P. putida and P. putida + A. calcoaceticus resulted in 15% higher yield compared to the control. Similarly, field trials indicated that P. putida improved the values of investigated traits by 12.3-37.2%. Pseudomonas putida is considered beneficial for future research based on the results of this study.
Etik Beyan
There is no need to obtain permission from the ethics committee for this study.
Destekleyen Kurum
This study was supported by Tokat Gaziosmanpaşa University Scientific Research Project Unit (Project No: 2016/77).
Kaynakça
- Belgüzar, S., Ciner, I., Eroglu, Z., & Yanar, Y., (2021). Effects Of Rhızobacterıa On Tomato Bacterıal Cancer And Wılt Dısease. Feb Fresenius Environmental Bulletin, 1075.
- Bigatton, E. D., Ayoub, I., Palmero, F., Castillejo, M. Á., Vázquez, C., Lucini, E. I., & Haro, R. J. (2024). Plant-growth promoting rhizobacteria on peanuts: Effects on yield determination, growth rates, and radiation use efficiency in field trials in Argentina. European Journal of Agronomy, 154, 127113.
- Felipe V., Bianco .MI., Terrestre M., Mielnichuk N., Romero A.M., & Yaryura P.M., (2021). Biocontrol of tomato bacterial spot by novel Bacillus and Pseudomonas strains, , Eur J Plant Pathol 160, 935–948, https://doi.org/10.1007/s10658-021-02297-6
- Fira D., Dimkić I., Berić T., Lozo J., & Stanković S., (2018). Biological control of plant pathogens by Bacillus species, Journal of Biotechnology 285, 44–55
- Hardoim, P. R., van Overbeek, L. S. & Elsas, J. D. (2008) Properties of bacterial endophytes and their proposed role in plant growth. Trends Microbiol. 16, 463–471
- Kang, S. H., Soo Choh., Cheong H., Mınryu C., Kım J. F. & Park S.H. (2007), Two bacterial entophytes eliciting both plant growth promotion and plant defense on pepper (Capsicum annuum L.), J. Microbiol. Biotechnol., 17(1), 96–103
- Kang, S.M., Khan, A.L., Humayun, M., Shinwari, Z. K., Kim, Y.H., Joo G.J., & Lee, I.J. (2012). Acinetobacter calcoaceticus ameliorated plant growth and influenced gibberellins and functional biochemicals. Pak. J. Bot., 44(1), 365-372
- Kayaaslan, Z. (2021). Tokat ili biber üretim alanlarında bakteriyel leke hastalığı etmeni (Xanthomonas euvesicatoria)’nin tanılanması, epidemiyolojisi ve biyolojik mücadelesi. Doktora Tezi, Tokat Gaziosmanpaşa Üniversitesi, Lisansüstü Eğitim Enstitüsü, Tokat, Türkiye.
- Khodabin, M., Soudaei Mashaei, S., Barzegar, R., & Ghasemi Ghahsare, M. (2023). Effect of plant growth-promoting rhizobacteria inoculation on soil characteristics, nutrients uptake and growth of bell pepper (Capsicum annum L.). Iranian Journal of Soil and Water Research, 54(1), 1-13.
- Kloepper, J. W., R. M. Zablotowicz, E. M. Tipping, & R. Lifshitz. (1991). Plant growth promoting mediated by bacterial rhizosphere colonizers. The Rhizosphere and Plant Growth, D. L. Keister and P. B. Cregan eds. Kluwer Academic Publishers, Netherlands. 315-326
- Mirik M, Aysan Y. & Cinar O. (2008). Biological control of bacterial spot disease of pepper with Bacillus strains. Turk J Agri For 32, 381–390
- Santoyo, G., Orozco-Mosqueda, M. D., & Govindappa, M. (2012). Mechanisms of biocontrol and plant growth-promoting activity in soil bacterial species of Bacillus and Pseudomonas: a review.Biocontrol Sci. Technol. 22, 855–872. https://doi.org/10.1016/j.micres.2008.08.007
- Shrestha A, Kim BS, & Parka DH, (2014). Biological control of bacterial spot disease and plant growth-promoting effects of lactic acid bacteria on pepper. Biocontrol Sci Tech 24(7):763–779. https://doi.org/10.1080/09583157.2014.894495
- Siddiqui, Z.A. (2005). PGPR: prospective biocontrol agents of plant pathogens. In: Siddiqui ZA (eds) PGPR: biocontrol and biofertilization, Springer, Dordrecht https://doi.org/10.1007/1-4020-4152-7_4
Öz
Bu çalışmada, 2017 yılında yapılan sürveyler sonucunda toplanan 389 aday bakteri izolatının bitki büyümesini teşvik edici özellikleri araştırılmıştır. Elde edilen izolatların bitki gelişimini teşvik etme özelliklerini belirlemek amacıyla azotu bağlama, fosforu indirgeme ve antagonistik etki testleri yapılmıştır. Testler sonucunda altı adet aday bakteri izolatı belirlenmiştir. Bu türler MALDİ-TOF MS tekniği ile Enterobacter cloacae, Enterobacter aerogenes, Bacillus cereus, Microbacterium testaceum, Pseudomonas putida ve Acinetobacter calcoaceticus bakterileri olduğu tanılanmıştır. Saksı ve tarla denemeleri, bu izolatların farklı kombinasyonları kullanılarak yürütülmüştür. Saksı denemesinde tüm izolatlar kullanılırken, tarla denemesinde en yüksek antagonistik etkiye sahip izolatlar ve bunların kombinasyonları kullanılmıştır. Saksı denemeleri tesadüf parselleri deneme desenine göre 5 tekerrür olarak yürütülmüştür. Tarla denemeleri ise tesadüf blokları deneme desenine göre 10 tekerrürlü yürütülmüştür. Bu izolatların sağlıklı biber bitkisinin verim değerlerine etkileri araştırılmıştır. Saksı çalışması ve devamında yürütülen tarla çalışmasında bitki boyu, kök uzunluğu, gövde çapı, meyve sayısı, bitki yaş ağırlığı, bitki kuru ağırlığı, kök yaş ağırlığı ve kök kuru ağırlığı gibi parametreler ölçülmüştür. Sonuç olarak sera denemelerinde B. cereus + P. putida ile P. putida + A. calcoaceticus bakterilerin ikili kombinasyon uygulamalarında kontrole göre %15 daha fazla verim elde edilmiştir. Tarla uygulamalarında ise P. putida uygulaması değerlendirmeye alınan bitki parametrelerinde %12,3-37,2 arasında değişen oranlarda artışı sağlamıştır. Çalışma sonuçlarına göre özellikle Pseudomonas putida ile ileriye dönük çalışmaların yapılmasının yararlı olacağı düşünülmektedir.
Kaynakça
- Belgüzar, S., Ciner, I., Eroglu, Z., & Yanar, Y., (2021). Effects Of Rhızobacterıa On Tomato Bacterıal Cancer And Wılt Dısease. Feb Fresenius Environmental Bulletin, 1075.
- Bigatton, E. D., Ayoub, I., Palmero, F., Castillejo, M. Á., Vázquez, C., Lucini, E. I., & Haro, R. J. (2024). Plant-growth promoting rhizobacteria on peanuts: Effects on yield determination, growth rates, and radiation use efficiency in field trials in Argentina. European Journal of Agronomy, 154, 127113.
- Felipe V., Bianco .MI., Terrestre M., Mielnichuk N., Romero A.M., & Yaryura P.M., (2021). Biocontrol of tomato bacterial spot by novel Bacillus and Pseudomonas strains, , Eur J Plant Pathol 160, 935–948, https://doi.org/10.1007/s10658-021-02297-6
- Fira D., Dimkić I., Berić T., Lozo J., & Stanković S., (2018). Biological control of plant pathogens by Bacillus species, Journal of Biotechnology 285, 44–55
- Hardoim, P. R., van Overbeek, L. S. & Elsas, J. D. (2008) Properties of bacterial endophytes and their proposed role in plant growth. Trends Microbiol. 16, 463–471
- Kang, S. H., Soo Choh., Cheong H., Mınryu C., Kım J. F. & Park S.H. (2007), Two bacterial entophytes eliciting both plant growth promotion and plant defense on pepper (Capsicum annuum L.), J. Microbiol. Biotechnol., 17(1), 96–103
- Kang, S.M., Khan, A.L., Humayun, M., Shinwari, Z. K., Kim, Y.H., Joo G.J., & Lee, I.J. (2012). Acinetobacter calcoaceticus ameliorated plant growth and influenced gibberellins and functional biochemicals. Pak. J. Bot., 44(1), 365-372
- Kayaaslan, Z. (2021). Tokat ili biber üretim alanlarında bakteriyel leke hastalığı etmeni (Xanthomonas euvesicatoria)’nin tanılanması, epidemiyolojisi ve biyolojik mücadelesi. Doktora Tezi, Tokat Gaziosmanpaşa Üniversitesi, Lisansüstü Eğitim Enstitüsü, Tokat, Türkiye.
- Khodabin, M., Soudaei Mashaei, S., Barzegar, R., & Ghasemi Ghahsare, M. (2023). Effect of plant growth-promoting rhizobacteria inoculation on soil characteristics, nutrients uptake and growth of bell pepper (Capsicum annum L.). Iranian Journal of Soil and Water Research, 54(1), 1-13.
- Kloepper, J. W., R. M. Zablotowicz, E. M. Tipping, & R. Lifshitz. (1991). Plant growth promoting mediated by bacterial rhizosphere colonizers. The Rhizosphere and Plant Growth, D. L. Keister and P. B. Cregan eds. Kluwer Academic Publishers, Netherlands. 315-326
- Mirik M, Aysan Y. & Cinar O. (2008). Biological control of bacterial spot disease of pepper with Bacillus strains. Turk J Agri For 32, 381–390
- Santoyo, G., Orozco-Mosqueda, M. D., & Govindappa, M. (2012). Mechanisms of biocontrol and plant growth-promoting activity in soil bacterial species of Bacillus and Pseudomonas: a review.Biocontrol Sci. Technol. 22, 855–872. https://doi.org/10.1016/j.micres.2008.08.007
- Shrestha A, Kim BS, & Parka DH, (2014). Biological control of bacterial spot disease and plant growth-promoting effects of lactic acid bacteria on pepper. Biocontrol Sci Tech 24(7):763–779. https://doi.org/10.1080/09583157.2014.894495
- Siddiqui, Z.A. (2005). PGPR: prospective biocontrol agents of plant pathogens. In: Siddiqui ZA (eds) PGPR: biocontrol and biofertilization, Springer, Dordrecht https://doi.org/10.1007/1-4020-4152-7_4
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Fitopatoloji |
| Bölüm | Araştırma Makaleleri |
| Yazarlar | |
| Yayımlanma Tarihi | 30 Nisan 2025 |
| Gönderilme Tarihi | 21 Kasım 2024 |
| Kabul Tarihi | 25 Şubat 2025 |
| Yayımlandığı Sayı | Yıl 2025 Cilt: 42 Sayı: 1 |
