Öz
Sentromer hedefli biyoteknoloji uygulamaları ve bitki sentromerlerinin kromozom mühendisliği deneyleri hassas sentetik bitki ıslahı ve ürünlerin genetik olarak iyileştirilmesinde yaygın olarak kullanılmaktadır. Bu bağlamda sentromer, kromozom yapısından genetik mühendisliği ve biyoteknoloji uygulamalarına geçişte en önemli kromozomal bölgedir. Sentromer pozisyonu ve karyotip formülü varyasyonları Aloe vera'da yaygındır ve bu çalışmada sentromerik pozisyona dayalı simetri/asimetri indeksi (S/AI) ile karyotip asimetrisinin hesaplanmasına odaklanılmıştır. Farklı S/AI değerleri 2,43 ile 2,71 arasında hesaplanmıştır. S/AI değerlerindeki farklılıklara rağmen Aloe vera'nın yalnızca simetrik ve simetrik arasında olan bir karyotip tipi vardı. Sonuç olarak, Aloe vera'da gözlenen karyolojik varyasyonların diğer Aloe türlerinde de görülebilmesi mümkündür. Sonuçlar Aloe cinsinin sito- ve biyoteknolojik verileri hakkında önemli katkılar sağlayacaktır.
Anahtar Kelimeler
Kaynakça
- Chaudhari, A. K., Chaudhary, B. R. (2012). Meiotic chromosome behaviour and karyomorphology of Aloe vera (L.) Burm. f. Chromosome Botany, 7, 23-29.
- Choi, S., Chung, M. H. (2003). A review on the relationship between Aloe vera components and their biologic effects. Seminars in Integrative Medicine, 1(1), 53-62.
- Das, A., Mukherjee, P., Ghorai, A., Jha, T. B. (2010). Comparative karyomorphological analyses of in vitro and in vivo grown plants of Aloe vera L. BURM. f. The Nucleus, 53, 89-94.
- Devi, S., Gupta, S., Sharma, N. (2019). Chromosome morphology and behaviour in Aloe vera L. plants growing at jammu, j&k state, India. Journal of Plant Development Sciences, 11(7), 403-407.
- Eroğlu, H. E. (2015). Which chromosomes are subtelocentric or acrocentric? A new karyotype symmetry/asymmetry index. Caryologia, 68(3), 239-245.
- Gunjan, K., Roy, B. K. (2010). Karyotype studies in dominant species of Aloe from eastern India. Caryologia, 63(1), 41-49.
- Haque, S. M., Ghosh, B. (2013). High frequency microcloning of Aloe vera and their true-to-type conformity by molecular cytogenetic assessment of two years old field growing regenerated plants. Botanical Studies, 54(1), 46 . Jahan, B., Vahidy, A. A., Saeed, R., Mirhabar, A. A. (2014). Karyological studies in ten different populations of desert lily Aloe vera from Pakistan. Pakistan Journal of Botany, 46(5), 1731-1734.
- Nejatzadeh-Barandozi, F., Akbari, L. (2013). Karyotypic variation of the Aloe vera L. and Aloe littoralis Baker in Iran. Iranian Journal of Biotechnology, 11(4), 233-237.
- Radha, M. H., Laxmipriya, N. P. (2014). Evaluation of biological properties and clinical effectiveness of Aloe vera: A systematic review. Journal of Traditional and Complementary Medicine, 5(1), 21-26.
- Sánchez, M., González-Burgos, E., Iglesias, I., Gómez-Serranillos, M. P. (2020). Pharmacological update properties of Aloe vera and its major active constituents. Molecules, 25(6), 1324.
- Taheri, J. B., Azimi, S., Rafieian, N., Zanjani, H. A. (2011). Herbs in dentistry. International Dental Journal, 61(6), 287-296.
- Yongzhong, W., Fan, P., Shuyu, S. (1998). Karyotype analysis of Aloe vera L. var. chinensis (Haw) Berger. Journal of Biology, 15(5), 42-43.
- Zhou, J., Liu, Y., Guo, X., Birchler, J. A., Han, F., Su, H. (2022). Centromeres: From chromosome biology to biotechnology applications and synthetic genomes in plants. Plant Biotechnology Journal, 20(11), 2051-2063.
Öz
The centromere-targeted biotechnology applications and the chromosome engineering experiments of plant centromeres are widely used in precision synthetic plant breeding and genetically improving crops. In this respect, the centromere is the most important chromosomal region in the transition from chromosome structure to genetic engineering and biotechnology applications. The variations of centromere position and karyotype formula are common in Aloe vera and in the present study, it is focused on the karyotype asymmetry calculation by the symmetry/asymmetry index (S/AI) based on the centromeric position. The different S/AI values were calculated from 2.43 to 2.71. Despite the differences in S/AI values, Aloe vera had only one karyotype types, which was between symmetric and symmetric. As a result, it is possible that the karyological variations observed in Aloe vera can also be seen in other Aloe species. The results will provide the important contributions on the cyto- and biotechnological data of the genus Aloe.
Anahtar Kelimeler
Kaynakça
- Chaudhari, A. K., Chaudhary, B. R. (2012). Meiotic chromosome behaviour and karyomorphology of Aloe vera (L.) Burm. f. Chromosome Botany, 7, 23-29.
- Choi, S., Chung, M. H. (2003). A review on the relationship between Aloe vera components and their biologic effects. Seminars in Integrative Medicine, 1(1), 53-62.
- Das, A., Mukherjee, P., Ghorai, A., Jha, T. B. (2010). Comparative karyomorphological analyses of in vitro and in vivo grown plants of Aloe vera L. BURM. f. The Nucleus, 53, 89-94.
- Devi, S., Gupta, S., Sharma, N. (2019). Chromosome morphology and behaviour in Aloe vera L. plants growing at jammu, j&k state, India. Journal of Plant Development Sciences, 11(7), 403-407.
- Eroğlu, H. E. (2015). Which chromosomes are subtelocentric or acrocentric? A new karyotype symmetry/asymmetry index. Caryologia, 68(3), 239-245.
- Gunjan, K., Roy, B. K. (2010). Karyotype studies in dominant species of Aloe from eastern India. Caryologia, 63(1), 41-49.
- Haque, S. M., Ghosh, B. (2013). High frequency microcloning of Aloe vera and their true-to-type conformity by molecular cytogenetic assessment of two years old field growing regenerated plants. Botanical Studies, 54(1), 46 . Jahan, B., Vahidy, A. A., Saeed, R., Mirhabar, A. A. (2014). Karyological studies in ten different populations of desert lily Aloe vera from Pakistan. Pakistan Journal of Botany, 46(5), 1731-1734.
- Nejatzadeh-Barandozi, F., Akbari, L. (2013). Karyotypic variation of the Aloe vera L. and Aloe littoralis Baker in Iran. Iranian Journal of Biotechnology, 11(4), 233-237.
- Radha, M. H., Laxmipriya, N. P. (2014). Evaluation of biological properties and clinical effectiveness of Aloe vera: A systematic review. Journal of Traditional and Complementary Medicine, 5(1), 21-26.
- Sánchez, M., González-Burgos, E., Iglesias, I., Gómez-Serranillos, M. P. (2020). Pharmacological update properties of Aloe vera and its major active constituents. Molecules, 25(6), 1324.
- Taheri, J. B., Azimi, S., Rafieian, N., Zanjani, H. A. (2011). Herbs in dentistry. International Dental Journal, 61(6), 287-296.
- Yongzhong, W., Fan, P., Shuyu, S. (1998). Karyotype analysis of Aloe vera L. var. chinensis (Haw) Berger. Journal of Biology, 15(5), 42-43.
- Zhou, J., Liu, Y., Guo, X., Birchler, J. A., Han, F., Su, H. (2022). Centromeres: From chromosome biology to biotechnology applications and synthetic genomes in plants. Plant Biotechnology Journal, 20(11), 2051-2063.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Bitki Biyoteknolojisi |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Yayımlanma Tarihi | 25 Nisan 2025 |
| Gönderilme Tarihi | 6 Şubat 2025 |
| Kabul Tarihi | 24 Şubat 2025 |
| Yayımlandığı Sayı | Yıl 2025 Cilt: 1 Sayı: 1 |
