Öz
Kaynakça
- Abdullah M. Z. (2008). Image Acquisition Systems. In: Sun, D.W. (Ed). Computer Vision Technology for Food Quality Evaluation 2nd edition, (pp. 3-43). Amsterdam: Academic Press.
- De Magalhaes Filho C. D., Henriquez B., Seah N. E., Evans R. M., & Lapierre L. R. (2018). Visible light reduces C. elegans longevity. Nature Communications 9(1), 927.
- Ghosh D. D., Lee D., Jin X., Horvitz H. R., & Nitabach M. N. (2021). C. elegans discriminates colors to guide foraging. Science, 371 (6533), 1059-1063.
- Johkan M., Shoji K., Goto F., Hahida S., & Yoshihara T. (2012). Effect of green light wavelength and intensity on photomorphogenesis and photosynthesis in Lactuca sativa. Environmental and Experimental Botany, 75, 128-133.
- Lee G.Y., & Lee S.V. (2021). Eyeless Worms Can Run Away from Dangerous Blues. Mol Cells, 44(8), 623-625. Leung M. C. K., Rooney J. P., Ryde I. T., Bernal A. J., & Bess A. S (2013). Effects of early life exposure to ultraviolet C radiation on mitochondrial DNA content, transcription, ATP production, and oxygen consumption in developing Caenorhabditis elegans. BMC Pharmacology and Toxicology, 14 (1), 9.
- Meneely P.M., Dahlberg C.L., & Rose J.K. (2019). Working with worms: Caenorhabditis elegans as a model organism. Current Protocols Essential Laboratory Techniques, 19 (1): e35.
- Ozawa K., Shinkai Y., Kako K., Fukamizu A., & Doi M. (2022). The molecular and neural regulation of ultraviolet light phototaxis and its food-associated learning behavioral plasticity in C. elegans. Neuroscience Letters, 770, 136384.
- Ward A., Liu J., Feng Z., & Xu X.S. (2008). Light-sensitive neurons and channels mediate phototaxis in C. elegans. Nature neuroscience, 11 (8): 916-922.
- Yang J., Nam, H.J., Seo M., Han, S.K., & Choi Y., (2011). OASIS: online application for the survival analysis of lifespan assays performed in aging research. PloS one, 6(8): e23525.
Öz
Caenorhabditis elegans, due to its short life cycle, transparency and known complete genetic map, is a frequently preferred experimental animal in recent years. Standardization of the laboratory condition is very important. Sometimes, these conditions may have unknown or unpredictable effects on the experimental animal we use. Light is a factor that is often overlooked while conditions such as temperature and humidity of the environment are always in the foreground. Our aim was to find the most compatible light color for a caenorhabditis elegans, also searching if what range of colors would have effect on these worms’ life span. Eggs were collected from adult C. elegans for standardization and then waited for each group to hatch under their own light color conditions (dark, day light, red, blue, green). After the C. elegans became adults, they were subjected to the life span analysis under their own light colors. The experiments continued until the last nematode died and the results were reported via statistical methods for survival analyses using an online application. Red and blue light colors have shortened the worms’ life span otherwise there were nematodes that did not show mortality in the first two phases of green light environment. Nematodes were found to be more successful in survival experiments in dark and light environments that were close to their natural habitats; green light showed similar results with day light and darkness. The survival rates of adult nematodes that completed their development under red and blue light was found to be statistically low when compared to other light colors. The color that really surprised us was the green light; the survival rates of nematodes that completed their development under green light were quite successful in the first two series compared to red and blue colors.
Anahtar Kelimeler
Destekleyen Kurum
TÜBİTAK
Kaynakça
- Abdullah M. Z. (2008). Image Acquisition Systems. In: Sun, D.W. (Ed). Computer Vision Technology for Food Quality Evaluation 2nd edition, (pp. 3-43). Amsterdam: Academic Press.
- De Magalhaes Filho C. D., Henriquez B., Seah N. E., Evans R. M., & Lapierre L. R. (2018). Visible light reduces C. elegans longevity. Nature Communications 9(1), 927.
- Ghosh D. D., Lee D., Jin X., Horvitz H. R., & Nitabach M. N. (2021). C. elegans discriminates colors to guide foraging. Science, 371 (6533), 1059-1063.
- Johkan M., Shoji K., Goto F., Hahida S., & Yoshihara T. (2012). Effect of green light wavelength and intensity on photomorphogenesis and photosynthesis in Lactuca sativa. Environmental and Experimental Botany, 75, 128-133.
- Lee G.Y., & Lee S.V. (2021). Eyeless Worms Can Run Away from Dangerous Blues. Mol Cells, 44(8), 623-625. Leung M. C. K., Rooney J. P., Ryde I. T., Bernal A. J., & Bess A. S (2013). Effects of early life exposure to ultraviolet C radiation on mitochondrial DNA content, transcription, ATP production, and oxygen consumption in developing Caenorhabditis elegans. BMC Pharmacology and Toxicology, 14 (1), 9.
- Meneely P.M., Dahlberg C.L., & Rose J.K. (2019). Working with worms: Caenorhabditis elegans as a model organism. Current Protocols Essential Laboratory Techniques, 19 (1): e35.
- Ozawa K., Shinkai Y., Kako K., Fukamizu A., & Doi M. (2022). The molecular and neural regulation of ultraviolet light phototaxis and its food-associated learning behavioral plasticity in C. elegans. Neuroscience Letters, 770, 136384.
- Ward A., Liu J., Feng Z., & Xu X.S. (2008). Light-sensitive neurons and channels mediate phototaxis in C. elegans. Nature neuroscience, 11 (8): 916-922.
- Yang J., Nam, H.J., Seo M., Han, S.K., & Choi Y., (2011). OASIS: online application for the survival analysis of lifespan assays performed in aging research. PloS one, 6(8): e23525.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Veteriner Cerrahi |
| Bölüm | Araştırma Makaleleri |
| Yazarlar | |
| Yayımlanma Tarihi | 30 Nisan 2025 |
| Gönderilme Tarihi | 25 Eylül 2024 |
| Kabul Tarihi | 12 Ocak 2025 |
| Yayımlandığı Sayı | Yıl 2025 Cilt: 9 Sayı: 1 |
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