-karnitin, Koenzim Q10 ve Beta Karoten diyet takviyelerinin antioksidan ve antiradikal aktivitelerinin değerlendirilmesi
Öz
Bu çalışmada, L-karnitin, Koenzim Q10 (Co-Q10) ve β-karoten diyet takviyelerinin antioksidan aktivitelerini ölçmek için 2,2-difenil-l-pikrilhidrazil (DPPH) deneyi, süperoksit anyon (O·̄2) radikali ve hidrojen peroksit (H2O2) temizleme aktivitesi gibi yaygın testler kullanılmıştır. Bu testler ile antiradikal ve antioksidan kapasiteleri karşılaştırılarak değerlendirilmiştir. En yüksek serbest radikal temizleme aktivitesi β-karoten de (H2O2 %73; O·̄2 %69) gözlenirken, en düşük aktivite L-karnitin (H2O2 %59; O·̄2 %67) kaydedildi. Bu diyet takviyelerinin radikalleri temizleme aktivitesi birbirinden oldukça farklı olduğu kaydedilmiştir (P < 0.05). Bu diyet takviyelerinin antiradikal aktiviteleri kararlı DPPH radikalini temizleme yeteneği, kolorimetrik olarak ölçüldü. β-karotenin Co-Q10, L-karnitin ve metanol çözeltilerinin etkili konsantrasyonları (EC50) değerleri 30 dk sonra sırasıyla 10.060, 28,990 ve 146,682 μg/mL olarak ölçülmüştür. Bu sonuçlar, her üç diyet takviyesinin askorbik asit ile kıyaslandığıında güçlü antioksidan ve antiradikal aktivitelerini göstermektedir. Sadece, L-karnitinin antiradikal aktivitesinin diğerlerinde göre daha düşük olduğu söylenebilir, bu durumun ise kinetik davaranışa bağlı olabileceği akla getirmiştir.
Anahtar Kelimeler
Kaynakça
- Valko, M., Izakovic, M., Mazur, M., Rhodes, C. J., & Telser, J. (2004). Role of oxygen radicals in DNA damage and cancer incidence. Molecular and cellular biochemistry, 266, 37-56.
- Hanukoglu, I., Rapoport, R., Weiner, L., & Sklan, D. (1993). Electron leakage from the mitochondrial NADPH-adrenodoxin reductase-adrenodoxin-P450scc (cholesterol side chain cleavage) system. Archives of biochemistry and biophysics, 305(2), 489-498.
- Salvador, A., Sousa, J., & Pinto, R. E. (2001). Hydroperoxyl, superoxide and pH gradients in the mitochondrial matrix: a theoretical assessment. Free Radical Biology and Medicine, 31(10), 1208-1215.
- Benzi, G. I. A. N., Pastoris, O., Marzatico, F., Villa, R. F., Dagani, F., & Curti, D. (1992). The mitochondrial electron transfer alteration as a factor involved in the brain aging. Neurobiology of Aging, 13(3), 361-368.
- Brookes, P. S., Levonen, A. L., Shiva, S., Sarti, P., & Darley-Usmar, V. M. (2002). Mitochondria: regulators of signal transduction by reactive oxygen and nitrogen species. Free Radical Biology and Medicine, 33(6), 755-764
- de Grey, A. D. (2002). HO [sub 2]•: The Forgotten Radical. DNA & Cell Biology, 21(4).
- Valko, M., Rhodes, C. J. B., Moncol, J., Izakovic, M. M., & Mazur, M. (2006). Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chemico-biological interactions, 160(1), 1-40. https://doi.org/10.1016/j.cbi.2005.12.009
- Blois, M. S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181(4617), 1199-1200.
- Ernster, L., & Dallner, G. (1995). Biochemical, physiological and medical aspects of ubiquinone function. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1271(1), 195-204.
- Bhagavan, H. N., & Chopra, R. K. (2006). Coenzyme Q10: absorption, tissue uptake, metabolism and pharmacokinetics. Free radical research, 40(5), 445-453. https://doi.org/10.1080/10715760600617843
Assessing of antiradical and antioxidant activities of -carnitine, L-carnitine, Coenzyme Q10 and Beta Carotene dietary supplements
Öz
In this study, common tests such as 2,2-diphenyl-l-picrylhydrazyl (DPPH) assay, hydrogen peroxide (H2O2) and superoxide anion (O·̄2 ) radical scavenging activity were exerted to measure the antioxidant activities of L-carnitine, Coenzyme Q10 (Co-Q10) and β-carotene dietary supplements. Antiradical and antioxidant capacities were evaluated comparatively with these tests. The highest free radical scavenging activity was observed in β-carotene (H2O2 73%; O·̄2 69%), while the lowest activity was recorded in L-carnitine (H2O2 59%; O·̄2 67%). The radical scavenging activity of these dietary supplements was discovered to be significantly different from each other (P < 0,05). The antiradical activities of these dietary supplements were measured colorimetrically based on their DPPH radical scavenging ability. The effective concentrations (EC50) values of β-carotene, Co-Q10, L-carnitine and methanol solutions were measured as 10.060, 28.990 and 146.682 μg/mL after 30 min, respectively. These results demonstrate the potent antioxidant and antiradical activities of all three dietary supplements compared to ascorbic acid. It can only be said that the antiradical activity of L-carnitine is lower than the others, suggesting that this may be due to kinetic behavior.
Anahtar Kelimeler
Kaynakça
- Valko, M., Izakovic, M., Mazur, M., Rhodes, C. J., & Telser, J. (2004). Role of oxygen radicals in DNA damage and cancer incidence. Molecular and cellular biochemistry, 266, 37-56.
- Hanukoglu, I., Rapoport, R., Weiner, L., & Sklan, D. (1993). Electron leakage from the mitochondrial NADPH-adrenodoxin reductase-adrenodoxin-P450scc (cholesterol side chain cleavage) system. Archives of biochemistry and biophysics, 305(2), 489-498.
- Salvador, A., Sousa, J., & Pinto, R. E. (2001). Hydroperoxyl, superoxide and pH gradients in the mitochondrial matrix: a theoretical assessment. Free Radical Biology and Medicine, 31(10), 1208-1215.
- Benzi, G. I. A. N., Pastoris, O., Marzatico, F., Villa, R. F., Dagani, F., & Curti, D. (1992). The mitochondrial electron transfer alteration as a factor involved in the brain aging. Neurobiology of Aging, 13(3), 361-368.
- Brookes, P. S., Levonen, A. L., Shiva, S., Sarti, P., & Darley-Usmar, V. M. (2002). Mitochondria: regulators of signal transduction by reactive oxygen and nitrogen species. Free Radical Biology and Medicine, 33(6), 755-764
- de Grey, A. D. (2002). HO [sub 2]•: The Forgotten Radical. DNA & Cell Biology, 21(4).
- Valko, M., Rhodes, C. J. B., Moncol, J., Izakovic, M. M., & Mazur, M. (2006). Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chemico-biological interactions, 160(1), 1-40. https://doi.org/10.1016/j.cbi.2005.12.009
- Blois, M. S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181(4617), 1199-1200.
- Ernster, L., & Dallner, G. (1995). Biochemical, physiological and medical aspects of ubiquinone function. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1271(1), 195-204.
- Bhagavan, H. N., & Chopra, R. K. (2006). Coenzyme Q10: absorption, tissue uptake, metabolism and pharmacokinetics. Free radical research, 40(5), 445-453. https://doi.org/10.1080/10715760600617843
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Biyokimya ve Hücre Biyolojisi (Diğer) |
| Bölüm | Araştırma Makaleleri |
| Yazarlar | |
| Erken Görünüm Tarihi | 15 Nisan 2025 |
| Yayımlanma Tarihi | 15 Nisan 2025 |
| Gönderilme Tarihi | 18 Ekim 2024 |
| Kabul Tarihi | 17 Aralık 2024 |
| Yayımlandığı Sayı | Yıl 2025 Cilt: 8 Sayı: 1 |
