Kısa Bildiri
Effect of Dose-Dependent Application of Fungicides Propineb and Mancozeb on H2O2, Lipid Peroxidation, and Photosynthetic Pigment in Tomato Leaves
Öz
Objective: The study investigated the growth, photosynthetic pigment, hydrogen peroxide (H2O2), and malondialdehyde (MDA) contents of tomato leaves under different concentrations of two modern fungicides, mancozeb and propineb.
Materials and Methods: Tomato plants were cultivated for 45 days and irrigated with ¼ Hoagland solution. Three different concentrations of propineb and mancozeb; half of the recommended dose (1.5 g/L and 1 g/L), recommended dose (3 g/L and 2 g/L), and two times higher (6 g/L and 4 g/L) sprayed on tomato leaves, respectively. After the fungicide treatment, tomato leaves were harvested at 1, 3, and 7 days after the treatment (DAT).
Results: The highest doses of propineb and mancozeb inhibited shoot growth compared with the control at 7 DAT. The chlorophyll a, b and carotenoid contents were significantly reduced with all mancozeb and propineb treatment doses at 3 and 7 DAT. The phytotoxic effects of fungicides were determined by H2O2 and MDA content 1, 3, and 7 days after treatment in leaves. The foliar application of propineb and mancozeb altered the production of H2O2 and MDA, depending on the time and concentration. The analysis of the data revealed that the application of propineb and mancozeb at higher concentrations significantly increased H2O2 and MDAlevels, which caused toxicity in tomato leaves.
Conclusion: The findings revealed that higher doses of mancozeb and propineb fungicides exert phytotoxic effects by inhibiting growth and photosynthetic pigment production and increasing oxidative stress in tomato leaves.
Anahtar Kelimeler
Fungicides Tomato Cholorophyll Oxidative stress Malondialdehyde
Kaynakça
- Dias, MC Phytotoxicity: An overview of the physiological re-sponses of plants exposed to fungicides J Bot 2012;135479 doi org/10 1155/2012/135479 google scholar
- Gikas GD, Parlakidis P, Mavropoulos T, Vryzas Z Partic-ularities of fungicides and factors affecting their fate and removal efficacy: A Review Sustainability 2022;14:4056 doi org/10 3390/su14074056 google scholar
- FAO . Pesticides use and trade, 1990-2021. FAOSTAT Analytical Briefs Series No. 70. Rome, 2023: doi.org/10.4060/cc6958en google scholar
- Özercan B, Taşcı R. Investigation of pesticide use in Türkiye in terms of provinces, regions and pesticide groups. Ziraat Mühendisliği. 2022;375:75-88. google scholar
- Yücel S, Can C, Yurtmen M, Cetinkaya-Yildiz R, Aysan Y. Tomato pathology in Turkey. Eur J Plant Sci Biotechnol. 2008;2(1):38-47. google scholar
- Engindeniz S, Öztürk Coşar G. An economic comparison of pesti-cide applications for processing and table tomatoes: A case study for Turkey. J Plant Prot Res. 2013;53(3):230-237. google scholar
- Thind TS, Hollomon DW. Thiocarbamate fungicides: Reliable tools in resistance management and future outlook. Pest Manag Sci. 2018;74:1547-1551. google scholar
- Dewez D, Geoffroy L, Vernet G, Popovic R. Determination of photosynthetic and enzymatic biomarkers sensitivity used to eval-uate toxic effects of copper and fludioxonil in alga Scenedesmus obliquus. Aquat Toxicol. 2005;74:150-159. google scholar
- Petit AN, Fontaine F, Clement C, Vaillant-Gaveau N. Photosyn-thesis limitations of grapevine after treatment with the fungicide fludioxonil. J Agric Food Chem. 2008;13(15):6761-6767. google scholar
- Xia XJ, Huang YY, Wang L, et al. Pesticides-induced depression photosynthesis was alleviated by 24-epibrassinolide pretreatment in Cucumis sativus. Pestic Biochem and Physiol. 2006;86:42-48. google scholar
- Marques LN, Balardin RS, Stefanello MT, et al. Physiolog-ical, biochemical, and nutritional parameters of wheat ex-posed to fungicide and foliar fertilizer. Semin-Ciencias Agrar. 2016;37(3):1243-1254. google scholar
- Dias MC, Figueiredo P, Duarte IF, Gil AM, Santos C. Different responses of young and expanded lettuce leaves to fungicide Man-cozeb: Chlorophyll fluorescence, lipid peroxidation, pigments and proline content. Photosynthetica. 2014;52:148-151. google scholar
- Singh G, Sahota HK. Impact of benzimidazole and dithiocarba-mate fungicides on the photosynthetic machinery, sugar content and various antioxidative enzymes in chickpea. Plant Physiol Biochem. 2018;132:166-173. google scholar
- Gill SS, Tuteja N. Reactive oxygen species and antioxidant ma-chinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem. 2010;48(12):909-930. google scholar
- Liu R, Li J, Zhang L, Feng T, Zhang Z, Zhang B. Fungicide difenoconazole induced biochemical and developmental toxic-ity in wheat (Triticum aestivum L.). Plants. 2021;10(11):2304. doi.org/10.3390/plants10112304. google scholar
- Shahid M, Ahmed B, Zaidi A, Khan M S. Toxicity of fungicides to Pisum sativum: A study of oxidative damage, growth suppres-sion, cellular death and morpho-anatomical changes. RSC Adv. 2018;8:38483-38498. google scholar
- Lichtenthaler HK, Buschmann C. Chlorophylls and carotenoids: Measurement and characterization by UV-VIS spectroscopy. Curr Protoc Chem Biol. 2001; 1:F4.3.1-F4.3.8. google scholar
- Velikova V, Yordanov I, Edreva A. Oxidative stress and some antioxidant systems in acid rain-treated bean plants: Protective role of exogenous polyamines. Plant Sci. 2000;151:59-66. google scholar
- Jiang M, Zhang J. Effect of abscisic acid on active oxygen species, antioxidative defense system and oxidative damage in leaves of maize seedlings. Plant Cell Physiol. 2001;42:1265-1273. google scholar
- Parween T, Jan S, Mahmooduzzafar S, Fatma T, Siddiqui Z H. Selective effect of pesticides on plant—A Review. Crit Rev Food Sci Nutr. 2016;56(1):160-179. google scholar
- Yüzbaşıoğlu E, Dalyan E. Salicylic acid alleviates thiram toxicity by modulating antioxidant enzyme capacity and pesticide detoxif-cation systems in the tomato (Solanum lycopersicum Mill.). Plant Physiol Biochem. 2019;135:322-330. google scholar
- Pereira SI, Figueiredo PI, Barros AS, et al. Changes in the metabolome of lettuce leaves due to exposure to mancozeb pesti-cide. Food Chem. 2014;1(154):291-298. google scholar
- Shakir SK, Kanwal M, Murad W, et al. Effect of some commonly used pesticides on seed germination, biomass production and photosynthetic pigments in tomato (Lycopersicon esculentum). Ecotoxicol. 2016;25:329-341. google scholar
- Akter S, Khan MS, Smith EN, Flashman E. Measuring ROS and redox markers in plant cells. RSC Chem Biol. 2021;2:1384-1401. google scholar
- Sherin G, Aswathi KPR, Puthur JT. Photosynthetic functions in plants subjected to stresses are positively influenced by priming. Plant Stress. 2022;4:100079. doi 10.1016/j.stress.2022.100079. google scholar
- Kılıç S, Duran RE, Coskun Y. Morphological and physiological responses of maize (Zea mays L.) seeds grown under increasing concentrations of chlorantraniliprole insecticide. Pol J Environ Stud. 2015;24(3):1069-1075. google scholar
Yıl 2024,
Cilt: 83 Sayı: 2, 254 - 260, 19.12.2024
Öz
Kaynakça
- Dias, MC Phytotoxicity: An overview of the physiological re-sponses of plants exposed to fungicides J Bot 2012;135479 doi org/10 1155/2012/135479 google scholar
- Gikas GD, Parlakidis P, Mavropoulos T, Vryzas Z Partic-ularities of fungicides and factors affecting their fate and removal efficacy: A Review Sustainability 2022;14:4056 doi org/10 3390/su14074056 google scholar
- FAO . Pesticides use and trade, 1990-2021. FAOSTAT Analytical Briefs Series No. 70. Rome, 2023: doi.org/10.4060/cc6958en google scholar
- Özercan B, Taşcı R. Investigation of pesticide use in Türkiye in terms of provinces, regions and pesticide groups. Ziraat Mühendisliği. 2022;375:75-88. google scholar
- Yücel S, Can C, Yurtmen M, Cetinkaya-Yildiz R, Aysan Y. Tomato pathology in Turkey. Eur J Plant Sci Biotechnol. 2008;2(1):38-47. google scholar
- Engindeniz S, Öztürk Coşar G. An economic comparison of pesti-cide applications for processing and table tomatoes: A case study for Turkey. J Plant Prot Res. 2013;53(3):230-237. google scholar
- Thind TS, Hollomon DW. Thiocarbamate fungicides: Reliable tools in resistance management and future outlook. Pest Manag Sci. 2018;74:1547-1551. google scholar
- Dewez D, Geoffroy L, Vernet G, Popovic R. Determination of photosynthetic and enzymatic biomarkers sensitivity used to eval-uate toxic effects of copper and fludioxonil in alga Scenedesmus obliquus. Aquat Toxicol. 2005;74:150-159. google scholar
- Petit AN, Fontaine F, Clement C, Vaillant-Gaveau N. Photosyn-thesis limitations of grapevine after treatment with the fungicide fludioxonil. J Agric Food Chem. 2008;13(15):6761-6767. google scholar
- Xia XJ, Huang YY, Wang L, et al. Pesticides-induced depression photosynthesis was alleviated by 24-epibrassinolide pretreatment in Cucumis sativus. Pestic Biochem and Physiol. 2006;86:42-48. google scholar
- Marques LN, Balardin RS, Stefanello MT, et al. Physiolog-ical, biochemical, and nutritional parameters of wheat ex-posed to fungicide and foliar fertilizer. Semin-Ciencias Agrar. 2016;37(3):1243-1254. google scholar
- Dias MC, Figueiredo P, Duarte IF, Gil AM, Santos C. Different responses of young and expanded lettuce leaves to fungicide Man-cozeb: Chlorophyll fluorescence, lipid peroxidation, pigments and proline content. Photosynthetica. 2014;52:148-151. google scholar
- Singh G, Sahota HK. Impact of benzimidazole and dithiocarba-mate fungicides on the photosynthetic machinery, sugar content and various antioxidative enzymes in chickpea. Plant Physiol Biochem. 2018;132:166-173. google scholar
- Gill SS, Tuteja N. Reactive oxygen species and antioxidant ma-chinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem. 2010;48(12):909-930. google scholar
- Liu R, Li J, Zhang L, Feng T, Zhang Z, Zhang B. Fungicide difenoconazole induced biochemical and developmental toxic-ity in wheat (Triticum aestivum L.). Plants. 2021;10(11):2304. doi.org/10.3390/plants10112304. google scholar
- Shahid M, Ahmed B, Zaidi A, Khan M S. Toxicity of fungicides to Pisum sativum: A study of oxidative damage, growth suppres-sion, cellular death and morpho-anatomical changes. RSC Adv. 2018;8:38483-38498. google scholar
- Lichtenthaler HK, Buschmann C. Chlorophylls and carotenoids: Measurement and characterization by UV-VIS spectroscopy. Curr Protoc Chem Biol. 2001; 1:F4.3.1-F4.3.8. google scholar
- Velikova V, Yordanov I, Edreva A. Oxidative stress and some antioxidant systems in acid rain-treated bean plants: Protective role of exogenous polyamines. Plant Sci. 2000;151:59-66. google scholar
- Jiang M, Zhang J. Effect of abscisic acid on active oxygen species, antioxidative defense system and oxidative damage in leaves of maize seedlings. Plant Cell Physiol. 2001;42:1265-1273. google scholar
- Parween T, Jan S, Mahmooduzzafar S, Fatma T, Siddiqui Z H. Selective effect of pesticides on plant—A Review. Crit Rev Food Sci Nutr. 2016;56(1):160-179. google scholar
- Yüzbaşıoğlu E, Dalyan E. Salicylic acid alleviates thiram toxicity by modulating antioxidant enzyme capacity and pesticide detoxif-cation systems in the tomato (Solanum lycopersicum Mill.). Plant Physiol Biochem. 2019;135:322-330. google scholar
- Pereira SI, Figueiredo PI, Barros AS, et al. Changes in the metabolome of lettuce leaves due to exposure to mancozeb pesti-cide. Food Chem. 2014;1(154):291-298. google scholar
- Shakir SK, Kanwal M, Murad W, et al. Effect of some commonly used pesticides on seed germination, biomass production and photosynthetic pigments in tomato (Lycopersicon esculentum). Ecotoxicol. 2016;25:329-341. google scholar
- Akter S, Khan MS, Smith EN, Flashman E. Measuring ROS and redox markers in plant cells. RSC Chem Biol. 2021;2:1384-1401. google scholar
- Sherin G, Aswathi KPR, Puthur JT. Photosynthetic functions in plants subjected to stresses are positively influenced by priming. Plant Stress. 2022;4:100079. doi 10.1016/j.stress.2022.100079. google scholar
- Kılıç S, Duran RE, Coskun Y. Morphological and physiological responses of maize (Zea mays L.) seeds grown under increasing concentrations of chlorantraniliprole insecticide. Pol J Environ Stud. 2015;24(3):1069-1075. google scholar
Toplam 26 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Bitki Fizyolojisi |
| Bölüm | Kısa Bildiri |
| Yazarlar | |
| Yayımlanma Tarihi | 19 Aralık 2024 |
| Gönderilme Tarihi | 26 Eylül 2024 |
| Kabul Tarihi | 13 Kasım 2024 |
| Yayımlandığı Sayı | Yıl 2024 Cilt: 83 Sayı: 2 |
