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Yıl 2025, Cilt: 17 Sayı: 1, 1245 - 1251, 27.02.2025

Dilek Ulusoy Karatopuk

https://doi.org/10.37212/jcnos.1630887

Öz

Proje Numarası

-

Kaynakça

  • Abdallah KS, Hunt S, Abdullah SA, Mol BWJ, Youssef MA. (2020). How and Why to Define Unexplained Infertility? Semin Reprod Med. 38(1): 55–60. https://doi.org/10.1055/s-0040-1718709.
  • Afrough M, Nikbakht R, Hashemitabar M, Ghalambaz E, Amirzadeh S, Zardkaf A, Adham S, Mehdipour M,
  • Dorfeshan P. (2024). Association of Follicular Fluid Antioxidants Activity with Aging and In Vitro Fertilization Outcome: A Cross-Sectional Study. Int J Fertil. 18(2): 115–122. https://doi.org/10.22074/ijfs.2023.555601.1317.
  • Agarwal A, Gupta S, Sharma RK. (2005). Role of oxidative stress in female reproduction. Reprod Biol Endocrinol. 3: 28. https://doi.org/10.1186/1477-7827-3-28.
  • Alasmari WA, Edris F, Albar Z, Eskandar, MA, Sultan C, Alboush A, Alasmari A. (2018). Comparable Reproductive Outcomes of ICSI for Couples with Unexplained Infertility and Couples with Male Factor. Infertility. Middle East Fertil. Soc. J. https://doi.org/10.1016/j.mefs.2018.05.010
  • Bahadori MH. Sharami SH, Fakor F, Milani F, Pourmarzi D, Dalil-Heirati SF (2017). Level of Vitamin E in Follicular Fluid and Serum and Oocyte Morphology and Embryo Quality in Patients Undergoing IVF Treatment. J Family Reprod Health. 11(2): 74–81.
  • Bhardwaj JK, Panchal H, Saraf P. (2021). Ameliorating Effects of Natural Antioxidant Compounds on Female Infertility: a Review. Reprod Sci. 28(5): 1227–1256. https://doi.org/10.1007/s43032-020-00312-5.
  • Browne RW, Bloom MS, Shelly WB, Ocque AJ, Huddleston, HG, Fujimoto, VY. (2009). Follicular fluid high density lipoprotein-associated micronutrient levels are associated with embryo fragmentation during IVF. J Assist Reprod Genet. 26(11-12): 557–560. https://doi.org/10.1007/s10815-009-9367-x.
  • Petean CC, Ferriani RA, dos Reis RM, de Moura MD, Jordão AAJr, Navarro PA. (2008). Lipid peroxidation and vitamin E in serum and follicular fluid of infertile women with peritoneal endometriosis submitted to controlled ovarian hyperstimulation: a pilot study. Fertil Steril. 90(6): 2080–2085. https://doi.org/10.1016/j.fertnstert.2007.10.072.
  • Choi YS, Cho S, Seo SK, Park JH, Kim SH, Lee BS. (2015). Alteration in the intrafollicular thiol-redox system in infertile women with endometriosis. Reproduction. 149(2): 155–162. https://doi.org/10.1530/REP-14-0438.
  • Desai ID. (1984). Vitamin E analysis methods for animal tissues. Methods Enzymol. 105:138-147.
  • Fonseca BM, Cruz R, Pinto B, Costa L, Felgueira E, Oliveira, P, Casal S, Rebelo, I. (2023). Retinoic acid (all-trans) presents antioxidant properties within human ovary and reduces progesterone production by human granulosa cells. Syst Biol Reprod Med. 69(2): 129–141. https://doi.org/10.1080/19396368.2022.2120439.
  • Gode F, Akarsu S, Gunnur DZ, Tamer B, Isik AZ. (2019). Effect Follicular Fluid Vitamin A, E, D and B6 on Embryo Morphokinetics and Pregnancy Rates in Patients Receiving Assisted Reproduction. Gynecol Obstet Reprod Med. 25(2): 89-95. https://doi.org/10.21613/gorm.2018.860.
  • Jamro EL, Bloom MS, Browne RW, Kim K, Greenwood EA, Fujimoto VY. (2019). Preconception serum lipids and lipophilic micronutrient levels are associated with live birth rates after IVF. Reprod Biomed Online. 39(4): 665–673. https://doi.org/10.1016/j.rbmo.2019.06.004.
  • Jeremic A, Vasiljevic M, Mikovic Z, Bukumiric Z, Simic, P, Stanisavljevic T, Simic T, Djukic T. (2025). Oxidative Homeostasis in Follicular Fluid and Embryo Quality-A Pilot Study. Int J Mol Sci. 26(1): 388. https://doi.org/10.3390/ijms26010388.
  • Lawrence RA, Burk RF. (1976). Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Commun. 71: 952-958. https://doi.org/10.1016/0006-291x(76)90747-6.
  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. (1951). Protein measurement with the Folin- Phenol reagent. J Biol Chem. 193: 265-275.
  • Machtinger R, Racowsky C. (2013). Morphological systems of human embryo assessment and clinical evidence. Reprod Biomed Online. 26(3): 210–221. https://doi.org/10.1016/j.rbmo.2012.10.021.
  • Mehendale SS, Kilari BAS, Deshmukh CS, Dhorepatil, BS, Nimbargi VN, Joshi SR. (2009). Oxidative stress-mediated essential polyunsaturated fatty acid alterations in female infertility. Hum Fertil. 12(1): 28–33. https://doi.org/10.1080/14647270802298280.
  • Melo AS, Kliemchen, J, Junior AA, Ferriani RA, Navarro PA. (2016). Oxidative stress and polycystic ovary syndrome: evaluation during ovarian stimulation for ICSI. Reproduction. https://doi.org/10.1530/REP-16-0084.
  • Nishihara T, Matsumoto K, Hosoi Y, Morimoto Y. (2018). Evaluation of antioxidant status and oxidative stress markers in follicular fluid for human in vitro fertilization outcome. Reprod Med Biol. 17(4): 481–486. https://doi.org/10.1002/rmb2.12229.
  • Paszkowski T, Traub AI, Robinson SY, McMaster D. (1995). Selenium dependent glutathione peroxidase activity in human follicular fluid. Clin Chim Acta. 236(2): 173–180. https://doi.org/10.1016/0009-8981(95)98130-9.
  • Pekel A, Gönenç A, Turhan NÖ, Kafalı H. (2015). Changes of sFas and sFasL, oxidative stress markers in serum and follicular fluid of patients undergoing IVF. J Assist Reprod Genet. 32(2): 233–241. https://doi.org/10.1007/s10815-014-0396-8.
  • Placer ZA, Cushman L, Johnson BC. (1966). Estimation of products of lipid peroxidation (malonyl dialdehyde) in biological fluids. Anal Biochem. 16: 359-364. https://doi.org/10.1016/0003-2697(66)90167-9.
  • Prieto L, Quesada JF, Cambero O, Pacheco A, Pellicer A, Codoceo R, Garcia VJA. (2012). Analysis of follicular fluid and serum markers of oxidative stress in women with infertility related to endometriosis. Fertil Steril. 98(1): 126–130. https://doi.org/10.1016/j.fertnstert.2012.03.052.
  • Schweigert FJ, Steinhagen B, Raila, J, Siemann, A, Peet D, Buscher U. (2003). Concentrations of carotenoids, retinol and alpha-tocopherol in plasma and follicular fluid of women undergoing IVF. Hum Reprod. 18(6): 1259–1264. https://doi.org/10.1093/humrep/deg249.
  • Skowrońska P, Kunicki M, Pastuszek E, Konieczna L, Bączek T, Łukaszuk K. (2020). Follicular fat-soluble vitamins as markers of oocyte competency. Syst Biol Reprod Med. 66(2): 112–121. https://doi.org/10.1080/19396368.2020.1718244.
  • Suzuki J, Katoh N. (1990). A simple and cheap method for measuring vitamin A in cattle using only a spectrophotometer. Jpn J Vet Sci. 52: 1282-1284.
  • Şentürk R, Tola EN, Bozkurt M, Doğuç DK. (2022). The role of oxidant status on the etiopathogenesis of unexplained infertility and intracytoplasmic sperm injection - embryo transfer success: a case-control study. J Obstet Gynaecol. 42(5): 1312–1318. https://doi.org/10.1080/01443615.2021.1960294.
  • Tiboni GM, Bucciarelli T, Giampietro F, Sulpizio M, Di IC. (2004). Influence of cigarette smoking on vitamin E, vitamin A, beta-carotene and lycopene concentrations in human pre-ovulatory follicular fluid. Int J Immunopathol Pharmacol. 17(3): 389–393. https://doi.org/10.1177/039463200401700319.
  • Tural R, Karakaya C, Erdem M, Aykol Z, Karabacak RO, Kavutçu M. (2021). Investigation of oxidative stress status in cumulus cells in patıents with in vitro fertilization. Turk J Med Sci. 51(4): 1969–1975. https://doi.org/10.3906/sag-2104-188.
  • Vašková J, Klepcová Z, Špaková I, Urdzík P, Štofilová J, Bertková I, Kľoc M, Rabajdová M. (2023). The Importance of Natural Antioxidants in Female Reproduction. Antioxidants (Basel). 12(4):907. https://doi.org/10.3390/antiox12040907.
  • Zal F, Ahmadi P, Davari, M, Khademi F, Jahromi MA, Anvar Z, Jahromi BN. (2020). Glutathione-dependent enzymes in the follicular fluid of the first-retrieved oocyte and their impact on oocyte and embryos in polycystic ovary syndrome: A cross-sectional study. Int J Reprod Biomed. 18(6): 415–424. https://doi.org/10.18502/ijrm.v13i6.7283.

The oocyte quality and follicular fluid lipid peroxidation and antioxidant levels in the patients undergoing in vitro fertilization treatment

Yıl 2025, Cilt: 17 Sayı: 1, 1245 - 1251, 27.02.2025

Dilek Ulusoy Karatopuk

https://doi.org/10.37212/jcnos.1630887

Öz

This study aimed to compare demographic, embryological, and biochemical parameters between male factor infertility and unexplained infertility groups undergoing IVF treatment. Demographic parameters such as age and BMI, along with embryological factors including the number of oocytes retrieved, MII oocytes, fertilization rate, and Grade II embryo quality, were evaluated. Biochemical markers, including MDA, GSH, GSH-Px, vitamin A, vitamin E, and ß-carotene, were also analyzed. No significant differences were observed between the two groups regarding demographic or embryological outcomes (p > 0.05), although Grade I embryo quality showed a significant difference (p = 0.047), underscoring the importance of embryo quality in IVF success. The clinical pregnancy and live birth rates were higher in the control group, but the differences were not statistically significant.

Further biochemical analysis revealed significantly higher MDA levels in the patient group, indicating increased oxidative stress (p < 0.001). Conversely, antioxidant levels, including GSH, GSH-Px, vitamin A, and vitamin E, were found to be lower in the patient group, suggesting a weakened defense against oxidative stress. These results highlight the critical role of oxidative stress and antioxidant status in IVF outcomes. Specifically, increased MDA levels and decreased antioxidant activity were linked to lower oocyte and embryo quality. Despite these findings, no significant differences were noted in pregnancy, clinical pregnancy, and birth rates between the groups.

In conclusion, this study emphasizes the importance of managing oxidative stress through antioxidant levels to potentially improve IVF success rates. Future research should further explore the biochemical parameters influencing IVF outcomes to refine treatment strategies for different infertility causes.

Anahtar Kelimeler

oocyte quality embryo quality lipid peroxidation vitamin E follicular fluid in vitro fertilization

Etik Beyan

The local human ethics committee of Suleyman Demirel University approved the project.

Destekleyen Kurum

-

Proje Numarası

-

Teşekkür

-

Kaynakça

  • Abdallah KS, Hunt S, Abdullah SA, Mol BWJ, Youssef MA. (2020). How and Why to Define Unexplained Infertility? Semin Reprod Med. 38(1): 55–60. https://doi.org/10.1055/s-0040-1718709.
  • Afrough M, Nikbakht R, Hashemitabar M, Ghalambaz E, Amirzadeh S, Zardkaf A, Adham S, Mehdipour M,
  • Dorfeshan P. (2024). Association of Follicular Fluid Antioxidants Activity with Aging and In Vitro Fertilization Outcome: A Cross-Sectional Study. Int J Fertil. 18(2): 115–122. https://doi.org/10.22074/ijfs.2023.555601.1317.
  • Agarwal A, Gupta S, Sharma RK. (2005). Role of oxidative stress in female reproduction. Reprod Biol Endocrinol. 3: 28. https://doi.org/10.1186/1477-7827-3-28.
  • Alasmari WA, Edris F, Albar Z, Eskandar, MA, Sultan C, Alboush A, Alasmari A. (2018). Comparable Reproductive Outcomes of ICSI for Couples with Unexplained Infertility and Couples with Male Factor. Infertility. Middle East Fertil. Soc. J. https://doi.org/10.1016/j.mefs.2018.05.010
  • Bahadori MH. Sharami SH, Fakor F, Milani F, Pourmarzi D, Dalil-Heirati SF (2017). Level of Vitamin E in Follicular Fluid and Serum and Oocyte Morphology and Embryo Quality in Patients Undergoing IVF Treatment. J Family Reprod Health. 11(2): 74–81.
  • Bhardwaj JK, Panchal H, Saraf P. (2021). Ameliorating Effects of Natural Antioxidant Compounds on Female Infertility: a Review. Reprod Sci. 28(5): 1227–1256. https://doi.org/10.1007/s43032-020-00312-5.
  • Browne RW, Bloom MS, Shelly WB, Ocque AJ, Huddleston, HG, Fujimoto, VY. (2009). Follicular fluid high density lipoprotein-associated micronutrient levels are associated with embryo fragmentation during IVF. J Assist Reprod Genet. 26(11-12): 557–560. https://doi.org/10.1007/s10815-009-9367-x.
  • Petean CC, Ferriani RA, dos Reis RM, de Moura MD, Jordão AAJr, Navarro PA. (2008). Lipid peroxidation and vitamin E in serum and follicular fluid of infertile women with peritoneal endometriosis submitted to controlled ovarian hyperstimulation: a pilot study. Fertil Steril. 90(6): 2080–2085. https://doi.org/10.1016/j.fertnstert.2007.10.072.
  • Choi YS, Cho S, Seo SK, Park JH, Kim SH, Lee BS. (2015). Alteration in the intrafollicular thiol-redox system in infertile women with endometriosis. Reproduction. 149(2): 155–162. https://doi.org/10.1530/REP-14-0438.
  • Desai ID. (1984). Vitamin E analysis methods for animal tissues. Methods Enzymol. 105:138-147.
  • Fonseca BM, Cruz R, Pinto B, Costa L, Felgueira E, Oliveira, P, Casal S, Rebelo, I. (2023). Retinoic acid (all-trans) presents antioxidant properties within human ovary and reduces progesterone production by human granulosa cells. Syst Biol Reprod Med. 69(2): 129–141. https://doi.org/10.1080/19396368.2022.2120439.
  • Gode F, Akarsu S, Gunnur DZ, Tamer B, Isik AZ. (2019). Effect Follicular Fluid Vitamin A, E, D and B6 on Embryo Morphokinetics and Pregnancy Rates in Patients Receiving Assisted Reproduction. Gynecol Obstet Reprod Med. 25(2): 89-95. https://doi.org/10.21613/gorm.2018.860.
  • Jamro EL, Bloom MS, Browne RW, Kim K, Greenwood EA, Fujimoto VY. (2019). Preconception serum lipids and lipophilic micronutrient levels are associated with live birth rates after IVF. Reprod Biomed Online. 39(4): 665–673. https://doi.org/10.1016/j.rbmo.2019.06.004.
  • Jeremic A, Vasiljevic M, Mikovic Z, Bukumiric Z, Simic, P, Stanisavljevic T, Simic T, Djukic T. (2025). Oxidative Homeostasis in Follicular Fluid and Embryo Quality-A Pilot Study. Int J Mol Sci. 26(1): 388. https://doi.org/10.3390/ijms26010388.
  • Lawrence RA, Burk RF. (1976). Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Commun. 71: 952-958. https://doi.org/10.1016/0006-291x(76)90747-6.
  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. (1951). Protein measurement with the Folin- Phenol reagent. J Biol Chem. 193: 265-275.
  • Machtinger R, Racowsky C. (2013). Morphological systems of human embryo assessment and clinical evidence. Reprod Biomed Online. 26(3): 210–221. https://doi.org/10.1016/j.rbmo.2012.10.021.
  • Mehendale SS, Kilari BAS, Deshmukh CS, Dhorepatil, BS, Nimbargi VN, Joshi SR. (2009). Oxidative stress-mediated essential polyunsaturated fatty acid alterations in female infertility. Hum Fertil. 12(1): 28–33. https://doi.org/10.1080/14647270802298280.
  • Melo AS, Kliemchen, J, Junior AA, Ferriani RA, Navarro PA. (2016). Oxidative stress and polycystic ovary syndrome: evaluation during ovarian stimulation for ICSI. Reproduction. https://doi.org/10.1530/REP-16-0084.
  • Nishihara T, Matsumoto K, Hosoi Y, Morimoto Y. (2018). Evaluation of antioxidant status and oxidative stress markers in follicular fluid for human in vitro fertilization outcome. Reprod Med Biol. 17(4): 481–486. https://doi.org/10.1002/rmb2.12229.
  • Paszkowski T, Traub AI, Robinson SY, McMaster D. (1995). Selenium dependent glutathione peroxidase activity in human follicular fluid. Clin Chim Acta. 236(2): 173–180. https://doi.org/10.1016/0009-8981(95)98130-9.
  • Pekel A, Gönenç A, Turhan NÖ, Kafalı H. (2015). Changes of sFas and sFasL, oxidative stress markers in serum and follicular fluid of patients undergoing IVF. J Assist Reprod Genet. 32(2): 233–241. https://doi.org/10.1007/s10815-014-0396-8.
  • Placer ZA, Cushman L, Johnson BC. (1966). Estimation of products of lipid peroxidation (malonyl dialdehyde) in biological fluids. Anal Biochem. 16: 359-364. https://doi.org/10.1016/0003-2697(66)90167-9.
  • Prieto L, Quesada JF, Cambero O, Pacheco A, Pellicer A, Codoceo R, Garcia VJA. (2012). Analysis of follicular fluid and serum markers of oxidative stress in women with infertility related to endometriosis. Fertil Steril. 98(1): 126–130. https://doi.org/10.1016/j.fertnstert.2012.03.052.
  • Schweigert FJ, Steinhagen B, Raila, J, Siemann, A, Peet D, Buscher U. (2003). Concentrations of carotenoids, retinol and alpha-tocopherol in plasma and follicular fluid of women undergoing IVF. Hum Reprod. 18(6): 1259–1264. https://doi.org/10.1093/humrep/deg249.
  • Skowrońska P, Kunicki M, Pastuszek E, Konieczna L, Bączek T, Łukaszuk K. (2020). Follicular fat-soluble vitamins as markers of oocyte competency. Syst Biol Reprod Med. 66(2): 112–121. https://doi.org/10.1080/19396368.2020.1718244.
  • Suzuki J, Katoh N. (1990). A simple and cheap method for measuring vitamin A in cattle using only a spectrophotometer. Jpn J Vet Sci. 52: 1282-1284.
  • Şentürk R, Tola EN, Bozkurt M, Doğuç DK. (2022). The role of oxidant status on the etiopathogenesis of unexplained infertility and intracytoplasmic sperm injection - embryo transfer success: a case-control study. J Obstet Gynaecol. 42(5): 1312–1318. https://doi.org/10.1080/01443615.2021.1960294.
  • Tiboni GM, Bucciarelli T, Giampietro F, Sulpizio M, Di IC. (2004). Influence of cigarette smoking on vitamin E, vitamin A, beta-carotene and lycopene concentrations in human pre-ovulatory follicular fluid. Int J Immunopathol Pharmacol. 17(3): 389–393. https://doi.org/10.1177/039463200401700319.
  • Tural R, Karakaya C, Erdem M, Aykol Z, Karabacak RO, Kavutçu M. (2021). Investigation of oxidative stress status in cumulus cells in patıents with in vitro fertilization. Turk J Med Sci. 51(4): 1969–1975. https://doi.org/10.3906/sag-2104-188.
  • Vašková J, Klepcová Z, Špaková I, Urdzík P, Štofilová J, Bertková I, Kľoc M, Rabajdová M. (2023). The Importance of Natural Antioxidants in Female Reproduction. Antioxidants (Basel). 12(4):907. https://doi.org/10.3390/antiox12040907.
  • Zal F, Ahmadi P, Davari, M, Khademi F, Jahromi MA, Anvar Z, Jahromi BN. (2020). Glutathione-dependent enzymes in the follicular fluid of the first-retrieved oocyte and their impact on oocyte and embryos in polycystic ovary syndrome: A cross-sectional study. Int J Reprod Biomed. 18(6): 415–424. https://doi.org/10.18502/ijrm.v13i6.7283.
Toplam 33 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Biyokimya ve Hücre Biyolojisi (Diğer)
Bölüm Original Articles
Yazarlar

Dilek Ulusoy Karatopuk 0000-0002-9984-294X

Proje Numarası -
Yayımlanma Tarihi 27 Şubat 2025
Gönderilme Tarihi 31 Ocak 2025
Kabul Tarihi 25 Şubat 2025
Yayımlandığı Sayı Yıl 2025 Cilt: 17 Sayı: 1

Kaynak Göster

APA Ulusoy Karatopuk, D. (2025). The oocyte quality and follicular fluid lipid peroxidation and antioxidant levels in the patients undergoing in vitro fertilization treatment. Journal of Cellular Neuroscience and Oxidative Stress, 17(1), 1245-1251. https://doi.org/10.37212/jcnos.1630887
AMA Ulusoy Karatopuk D. The oocyte quality and follicular fluid lipid peroxidation and antioxidant levels in the patients undergoing in vitro fertilization treatment. J Cell Neurosci Oxid Stress. Şubat 2025;17(1):1245-1251. doi:10.37212/jcnos.1630887
Chicago Ulusoy Karatopuk, Dilek. “The Oocyte Quality and Follicular Fluid Lipid Peroxidation and Antioxidant Levels in the Patients Undergoing in Vitro Fertilization Treatment”. Journal of Cellular Neuroscience and Oxidative Stress 17, sy. 1 (Şubat 2025): 1245-51. https://doi.org/10.37212/jcnos.1630887.
EndNote Ulusoy Karatopuk D (01 Şubat 2025) The oocyte quality and follicular fluid lipid peroxidation and antioxidant levels in the patients undergoing in vitro fertilization treatment. Journal of Cellular Neuroscience and Oxidative Stress 17 1 1245–1251.
IEEE D. Ulusoy Karatopuk, “The oocyte quality and follicular fluid lipid peroxidation and antioxidant levels in the patients undergoing in vitro fertilization treatment”, J Cell Neurosci Oxid Stress, c. 17, sy. 1, ss. 1245–1251, 2025, doi: 10.37212/jcnos.1630887.
ISNAD Ulusoy Karatopuk, Dilek. “The Oocyte Quality and Follicular Fluid Lipid Peroxidation and Antioxidant Levels in the Patients Undergoing in Vitro Fertilization Treatment”. Journal of Cellular Neuroscience and Oxidative Stress 17/1 (Şubat 2025), 1245-1251. https://doi.org/10.37212/jcnos.1630887.
JAMA Ulusoy Karatopuk D. The oocyte quality and follicular fluid lipid peroxidation and antioxidant levels in the patients undergoing in vitro fertilization treatment. J Cell Neurosci Oxid Stress. 2025;17:1245–1251.
MLA Ulusoy Karatopuk, Dilek. “The Oocyte Quality and Follicular Fluid Lipid Peroxidation and Antioxidant Levels in the Patients Undergoing in Vitro Fertilization Treatment”. Journal of Cellular Neuroscience and Oxidative Stress, c. 17, sy. 1, 2025, ss. 1245-51, doi:10.37212/jcnos.1630887.
Vancouver Ulusoy Karatopuk D. The oocyte quality and follicular fluid lipid peroxidation and antioxidant levels in the patients undergoing in vitro fertilization treatment. J Cell Neurosci Oxid Stress. 2025;17(1):1245-51.
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