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COTA PESTALOZZAE BOISS TOPRAK ÜSTÜ KISIMLARININ FENOLİK İÇERİĞİ VE ANTİBAKTERİYEL AKTİVİTESİ

Yıl 2025, Cilt: 49 Sayı: 2, 2 - 2

Özge Yılmaz , Sunasibel Rızvanoğlu , Özlem Bahadır Acıkara

https://doi.org/10.33483/jfpau.1572365

Öz

Amaç: Cota cinsi, Anthemis cinsinin alt türleri arasında sınıflandırılırken günümüzde ayrı bir cins olarak kabul edilmektedir. Türkiye'de de endemik türleri bulunan Cota cinsine ait türler literatürde diğer Asteracea bitkilerine kıyasla daha az çalışılmıştır. Bu çalışmada, Türkiye'de endemik bir tür olan C. pestalozzae Boiis. türünün fenolik içeriği ve antibakteriyel aktivitesinin araştırılması amaçlanmıştır.
Gereç ve Yöntem: C. pestalozzae Boiss. türünün toprak üstü kısmından hazırlanan metanollü ekstrenin fenolik içeriği Yüksek Performanslı Sıvı Kromatografisi (HPLC) analizi ile kalitatif ve kantitatif olarak analiz edilmiştir. Bu çalışmada, tespit edilen bileşiklerin her biri için tespit limiti ve tayin limiti hesaplanmıştır. Ayrıca ekstrenin antibakteriyel aktivitesi bazı Gram (+) ve (-) bakteri türlerine karşı araştırılmıştır.
Sonuç ve Tartışma: Yapılan çalışma, C. pestalozzae Boiss. bitkisinin toprak üstü kısmının metanollü ekstresinde klorojenik asit, 4,5-O-dikafeoil kinik asit, 3,5-O-dikafeoil kinik asit, rutin, hiperozit ve izokersetin kalitatif ve kantitatif olarak tespit edilmiştir. C. pestalozzae toprak üstü kısmı ekstresi sadece Staphylococcus aureus ATCC 43300'e (MRSA) karşı 10000 µg/ml MIC değeri ile antibakteriyel aktivite sergilemiştir. Çalışma sonuçları C. pestalozzae'nin fenolik bileşikler içerdiğini ve Gram (+) bakterilere karşı etkili olduğu göstermiştir.

Anahtar Kelimeler

Antibakteriyel aktivite Asteraceae Cota fenolik asitler flavonoitler HPLC

Kaynakça

  • 1. Presti, R.M.L., Oppolzer, S., Oberprieler, C. (2010). A molecular phylogeny and a revised classification of the Mediterranean genus Anthemis sl (Compositae, Anthemideae) based on three molecular markers and micromorphological characters. Taxon, 59(5), 1441-1456. [CrossRef]
  • 2. Özbek, M.U., Özbek, F., Vural, M. (2018). Achene morphology of the genus Cota J. Gay (Asteraceae) from Turkey and its taxonomic significance. Turkish Journal of Botany, 42(2), 208-223. [CrossRef]
  • 3. Koyuncu, O., Erkara, Í.P., Yaylaci, Ö.K., Ocak, A., Osoydan, K., Özgisi, K. (2013). Biology of rediscovered rare endemic Cota fulvida (Grierson) Houlb. in Eskişehir-Turkey. Bangladesh Journal of Botany, 42(1), 91-98. [CrossRef]
  • 4. Özek, G., Özbek, M.U., Arslan, M. (2018). Lipid and essential oil constituents of Cota hamzaoglui (Asteraceae). Journal of the Turkish Chemical Society Section A: Chemistry, 5(3), 1361-1370. [CrossRef]
  • 5. Papaioannou, P., Lazari, D., Karioti, A., Souleles, C., Heilmann, J., Hadjipavlou-Litina, D., Skalts, H. (2007). Phenolic compounds with antioxidant activity from Anthemis tinctoria L.(Asteraceae). Zeitschrift für Naturforschung C, 62(5-6), 326-330. [CrossRef]
  • 6. Shamloo, S., Jafari Marandi, S., Tajadod, G., Majd, A., Rahimi, R. (2022). Cytotoxic effect of hydroalcoholic extract of Cota tinctoria (L.) J. Gay on AGS and Hep-G2 cancer cell lines. Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas, 21(1), 108-122. [CrossRef]
  • 7. Özek, G., Özbek, M., Yur, S., Göger, F., Arslan, M., Özek, T. (2019). Assessment of endemic Cota fulvida (Asteraceae) for phytochemical composition and inhibitory activities against oxidation, alpha-amylase, lipoxygenase, xanthine oxidase and tyrosinase enzymes. Records of Natural Products, 13(4), 333-345. [CrossRef]
  • 8. Karadeniz, A., Çinbilgel, İ., Gün, S.Ş., Çetin, A. (2015). Antioxidant activity of some Turkish medicinal plants. Natural Product Research, 29(24), 2308-2312. [CrossRef]
  • 9. Göger, G., Allak, M., Şen, A., Göger, F., Tekin, M., Özek, G. (2021). Assessment of Cota altissima (L.) J. Gay for phytochemical composition and antioxidant, anti-inflammatory, antidiabetic and antimicrobial activities. Zeitschrift für Naturforschung C, 76(7-8), 317-327. [CrossRef]
  • 10. Erik, İ., Bozdal, G., Şener, S.Ö., Korkmaz, B., Karaoğlu, Ş. A., Terzioğlu, S., Yayli, N. (2022). Antimicrobial and lipase inhibition of essential oil and solvent extracts of Cota tinctoria var. tinctoria and characterization of the essential oil. Turkish Journal of Chemistry, 46(4), 1234-1244. [CrossRef]
  • 11. Bahadori, M.B., Zengin, G., Eskandani, M., Zali, A., Sadoughi, M.M., Ayatollahi, S.A. (2021). Determination of phenolics composition, antioxidant activity, and therapeutic potential of Golden marguerite (Cota tinctoria). Journal of Food Measurement and Characterization, 15, 3314-3322. [CrossRef]
  • 12. Talhouk, R.S., Nasr, B., Fares, M.B., Ajeeb, B., Nahhas, R., Al Aaraj, L., Talhouk, S.N., Ghaddar, T.H., Saliba, N.A. (2015). Anti-inflammatory and cytostatic activities of a parthenolide‐like sesquiterpene lactone from Cota palaestina subsp. syriaca. Evidence‐Based Complementary and Alternative Medicine, 2015(1), 474597. [CrossRef]
  • 13. Clinical and Laboratory Standards Institute (CLSI) Web site (2024). M07-A8 methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; Approved Standard, Nineteenth informational supplement, USA. Retrieved date January 2009, from https://clsi.org/. Accessed date: 03.04.2024.

PHENOLIC CONTENT AND ANTIBACTERIAL ACTIVITY OF COTA PESTALOZZAE BOISS. AERIAL PARTS

Yıl 2025, Cilt: 49 Sayı: 2, 2 - 2

Özge Yılmaz , Sunasibel Rızvanoğlu , Özlem Bahadır Acıkara

https://doi.org/10.33483/jfpau.1572365

Öz

Objective: The genus Cota was once considered a subgenus of Anthemis but is now recognized as an independent genus. The species belonging to Cota, including several endemic to Turkey, have been less extensively studied in the literature than other plants in the Asteraceae family. The current study aimed to examine the phenolic content and antibacterial activity of C. pestalozzae Boiss., an endemic species from Turkey.
Material and Method: The phenolic content of the methanol extract prepared from the aerial parts of C. pestalozzae Boiss. was analysed qualitatively and quantitatively using high-performance liquid chromatography (HPLC) analysis. This study calculated the limit of detection (LOD) and quantification (LOQ) for each detected compound. The antibacterial activity of the extract was also investigated against some Gram (+) and (-) bacteria strains.
Result and Discussion: In the current study, chlorogenic acid, 4,5-O-dicaffeoyl quinic acid, 3,5-O-dicaffeoyl quinic acid, rutin, hyperoside and isoquercetin were qualitatively and quantitatively detected in the methanolic extract of the aerial parts of C. pestalozzae Boiss. Extract of the aerial parts exhibited antibacterial activity only against Staphylococcus aureus ATCC 43300 (MRSA) with a MIC value of 10000 µg/ml. The research indicates that C. pestalozzae contains phenolic compounds and is effective against Gram (+) bacteria.

Anahtar Kelimeler

Antibacterial activity Asteraceae Cota flavonoids HPLC phenolic acids

Kaynakça

  • 1. Presti, R.M.L., Oppolzer, S., Oberprieler, C. (2010). A molecular phylogeny and a revised classification of the Mediterranean genus Anthemis sl (Compositae, Anthemideae) based on three molecular markers and micromorphological characters. Taxon, 59(5), 1441-1456. [CrossRef]
  • 2. Özbek, M.U., Özbek, F., Vural, M. (2018). Achene morphology of the genus Cota J. Gay (Asteraceae) from Turkey and its taxonomic significance. Turkish Journal of Botany, 42(2), 208-223. [CrossRef]
  • 3. Koyuncu, O., Erkara, Í.P., Yaylaci, Ö.K., Ocak, A., Osoydan, K., Özgisi, K. (2013). Biology of rediscovered rare endemic Cota fulvida (Grierson) Houlb. in Eskişehir-Turkey. Bangladesh Journal of Botany, 42(1), 91-98. [CrossRef]
  • 4. Özek, G., Özbek, M.U., Arslan, M. (2018). Lipid and essential oil constituents of Cota hamzaoglui (Asteraceae). Journal of the Turkish Chemical Society Section A: Chemistry, 5(3), 1361-1370. [CrossRef]
  • 5. Papaioannou, P., Lazari, D., Karioti, A., Souleles, C., Heilmann, J., Hadjipavlou-Litina, D., Skalts, H. (2007). Phenolic compounds with antioxidant activity from Anthemis tinctoria L.(Asteraceae). Zeitschrift für Naturforschung C, 62(5-6), 326-330. [CrossRef]
  • 6. Shamloo, S., Jafari Marandi, S., Tajadod, G., Majd, A., Rahimi, R. (2022). Cytotoxic effect of hydroalcoholic extract of Cota tinctoria (L.) J. Gay on AGS and Hep-G2 cancer cell lines. Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas, 21(1), 108-122. [CrossRef]
  • 7. Özek, G., Özbek, M., Yur, S., Göger, F., Arslan, M., Özek, T. (2019). Assessment of endemic Cota fulvida (Asteraceae) for phytochemical composition and inhibitory activities against oxidation, alpha-amylase, lipoxygenase, xanthine oxidase and tyrosinase enzymes. Records of Natural Products, 13(4), 333-345. [CrossRef]
  • 8. Karadeniz, A., Çinbilgel, İ., Gün, S.Ş., Çetin, A. (2015). Antioxidant activity of some Turkish medicinal plants. Natural Product Research, 29(24), 2308-2312. [CrossRef]
  • 9. Göger, G., Allak, M., Şen, A., Göger, F., Tekin, M., Özek, G. (2021). Assessment of Cota altissima (L.) J. Gay for phytochemical composition and antioxidant, anti-inflammatory, antidiabetic and antimicrobial activities. Zeitschrift für Naturforschung C, 76(7-8), 317-327. [CrossRef]
  • 10. Erik, İ., Bozdal, G., Şener, S.Ö., Korkmaz, B., Karaoğlu, Ş. A., Terzioğlu, S., Yayli, N. (2022). Antimicrobial and lipase inhibition of essential oil and solvent extracts of Cota tinctoria var. tinctoria and characterization of the essential oil. Turkish Journal of Chemistry, 46(4), 1234-1244. [CrossRef]
  • 11. Bahadori, M.B., Zengin, G., Eskandani, M., Zali, A., Sadoughi, M.M., Ayatollahi, S.A. (2021). Determination of phenolics composition, antioxidant activity, and therapeutic potential of Golden marguerite (Cota tinctoria). Journal of Food Measurement and Characterization, 15, 3314-3322. [CrossRef]
  • 12. Talhouk, R.S., Nasr, B., Fares, M.B., Ajeeb, B., Nahhas, R., Al Aaraj, L., Talhouk, S.N., Ghaddar, T.H., Saliba, N.A. (2015). Anti-inflammatory and cytostatic activities of a parthenolide‐like sesquiterpene lactone from Cota palaestina subsp. syriaca. Evidence‐Based Complementary and Alternative Medicine, 2015(1), 474597. [CrossRef]
  • 13. Clinical and Laboratory Standards Institute (CLSI) Web site (2024). M07-A8 methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; Approved Standard, Nineteenth informational supplement, USA. Retrieved date January 2009, from https://clsi.org/. Accessed date: 03.04.2024.
Toplam 13 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Farmakognozi
Bölüm Araştırma Makalesi
Yazarlar

Özge Yılmaz 0000-0003-1120-2006

Sunasibel Rızvanoğlu 0000-0003-4244-0920

Özlem Bahadır Acıkara 0000-0003-0809-784X

Erken Görünüm Tarihi 3 Mayıs 2025
Yayımlanma Tarihi
Gönderilme Tarihi 23 Ekim 2024
Kabul Tarihi 9 Aralık 2024
Yayımlandığı Sayı Yıl 2025 Cilt: 49 Sayı: 2

Kaynak Göster

APA Yılmaz, Ö., Rızvanoğlu, S., & Bahadır Acıkara, Ö. (2025). PHENOLIC CONTENT AND ANTIBACTERIAL ACTIVITY OF COTA PESTALOZZAE BOISS. AERIAL PARTS. Journal of Faculty of Pharmacy of Ankara University, 49(2), 2-2. https://doi.org/10.33483/jfpau.1572365
AMA Yılmaz Ö, Rızvanoğlu S, Bahadır Acıkara Ö. PHENOLIC CONTENT AND ANTIBACTERIAL ACTIVITY OF COTA PESTALOZZAE BOISS. AERIAL PARTS. Ankara Ecz. Fak. Derg. Mayıs 2025;49(2):2-2. doi:10.33483/jfpau.1572365
Chicago Yılmaz, Özge, Sunasibel Rızvanoğlu, ve Özlem Bahadır Acıkara. “PHENOLIC CONTENT AND ANTIBACTERIAL ACTIVITY OF COTA PESTALOZZAE BOISS. AERIAL PARTS”. Journal of Faculty of Pharmacy of Ankara University 49, sy. 2 (Mayıs 2025): 2-2. https://doi.org/10.33483/jfpau.1572365.
EndNote Yılmaz Ö, Rızvanoğlu S, Bahadır Acıkara Ö (01 Mayıs 2025) PHENOLIC CONTENT AND ANTIBACTERIAL ACTIVITY OF COTA PESTALOZZAE BOISS. AERIAL PARTS. Journal of Faculty of Pharmacy of Ankara University 49 2 2–2.
IEEE Ö. Yılmaz, S. Rızvanoğlu, ve Ö. Bahadır Acıkara, “PHENOLIC CONTENT AND ANTIBACTERIAL ACTIVITY OF COTA PESTALOZZAE BOISS. AERIAL PARTS”, Ankara Ecz. Fak. Derg., c. 49, sy. 2, ss. 2–2, 2025, doi: 10.33483/jfpau.1572365.
ISNAD Yılmaz, Özge vd. “PHENOLIC CONTENT AND ANTIBACTERIAL ACTIVITY OF COTA PESTALOZZAE BOISS. AERIAL PARTS”. Journal of Faculty of Pharmacy of Ankara University 49/2 (Mayıs 2025), 2-2. https://doi.org/10.33483/jfpau.1572365.
JAMA Yılmaz Ö, Rızvanoğlu S, Bahadır Acıkara Ö. PHENOLIC CONTENT AND ANTIBACTERIAL ACTIVITY OF COTA PESTALOZZAE BOISS. AERIAL PARTS. Ankara Ecz. Fak. Derg. 2025;49:2–2.
MLA Yılmaz, Özge vd. “PHENOLIC CONTENT AND ANTIBACTERIAL ACTIVITY OF COTA PESTALOZZAE BOISS. AERIAL PARTS”. Journal of Faculty of Pharmacy of Ankara University, c. 49, sy. 2, 2025, ss. 2-2, doi:10.33483/jfpau.1572365.
Vancouver Yılmaz Ö, Rızvanoğlu S, Bahadır Acıkara Ö. PHENOLIC CONTENT AND ANTIBACTERIAL ACTIVITY OF COTA PESTALOZZAE BOISS. AERIAL PARTS. Ankara Ecz. Fak. Derg. 2025;49(2):2-.
 Kapak Resmi İndir

Kapsam ve Amaç

Ankara Üniversitesi Eczacılık Fakültesi Dergisi, açık erişim, hakemli bir dergi olup Türkçe veya İngilizce olarak farmasötik bilimler alanındaki önemli gelişmeleri içeren orijinal araştırmalar, derlemeler ve kısa bildiriler için uluslararası bir yayım ortamıdır. Bilimsel toplantılarda sunulan bildiriler supleman özel sayısı olarak dergide yayımlanabilir. Ayrıca, tüm farmasötik alandaki gelecek ve önceki ulusal ve uluslararası bilimsel toplantılar ile sosyal aktiviteleri içerir.