Araştırma Makalesi
BibTex RIS Kaynak Göster

Yıl 2025, Cilt: 29 Sayı: 2, 507 - 515, 08.04.2025

Ayşe Erdoğan

https://doi.org/10.12991/jrespharm.1660997

Öz

Kaynakça

  • [1] Greenlee RT, Murray T, Bolden S, Wingpo CA. Cancer statistics. Cancer J Clin. 2000; 50: 7-33. https://doi.org/10.3322/canjclin.50.1.7
  • [2] Roberts AB, Wakefield LM. The two faces of transforming growth factor beta in carcinogenesis. Proc Natl Acad Sci USA. 2003; 100: 8621-8623. https://doi.org/10.1073/pnas.1633291100
  • [3] Parkin DM, Bray F, Ferlay J, Pisani P. Global cancer statistics. CA Cancer J Clin. 2005; 55: 74-108. https://doi.org/10.3322/canjclin.55.2.74
  • [4] Jemal A, Siegel R, Ward E, Hao Y, Xu J, Murray T, Thun MJ. Cancer statistics. CA Cancer J Clin. 2008; 58: 71-96. https://doi.org/10.3322/CA.2007.0010
  • [5] Yurdakul AS, Çalışır HC, Demirağ F, Taci N, Öğretensoy M. The distribution of histological types of lung cancer (Analysis of 2216 cases). Toraks Dergisi. 2002; 3: 59-65.
  • [6] Bunn, PA Jr, Franklin W. Epidermal growth factor receptor expression, signal pathway, and inhibitors in non-small cell lung cancer. Semin Oncol. 2002; 29: 38-44. https://doi.org/10.1053/sonc.2002.35646
  • [7] Travis WD. Pathology of lung cancer. Clin Chest Med. 2011; 32: 669-692. https://doi.org/10.1016/j.ccm.2011.08.005
  • [8] Aygencel ŞG, Oğuzülgen İK, Öztürk C. Predictive and prognostic factors in long term survival of small cell lung cancer. Toraks Dergisi. 2005; 6: 81-84.
  • [9] Yang P, Allen MS, Aubry MC, Wampfler JA, Marks RS, Edell ES, Thibodeau S, Adjei AA, Jett J, Deschamps C. Clinical features of 5,628 primary lung cancer patients: experience at Mayo Clinic from 1997 to 2003. Chest. 2005; 128: 452-462. https://doi.org/10.1378/chest.128.1.452
  • [10] Jeremic B, Milicic B, Dagovic A, Aleksandrovic J, Nikolic N. Pretreatment clinical prognostic factors in patients with stage IV non- small cell lung cancer (NSCLC) treated with chemotherapy. J Cancer Res Clin Oncol. 2003; 129: 114-122. https://doi.org/10.1007/s00432-002-0408-4
  • [11] Vallieres E. Role of adjuvant systemic therapy for stage I NSCLC. Thorac Surg Clin. 2007; 17: 279-285. https://doi.org/10.1016/j.thorsurg.2007.03.001
  • [12] Assaf MH, Ali AA, Makboul MA, Beck JP, Anton R. Preliminary study of phenolic glycerosides from Origanum majorana; quantative estimation of arbutin; cytotoxic activity of hydroquinone. Planta Med. 1987; 53: 343-345. https://doi.org/10.1055/s-2006-962734
  • [13] Kelly WJ. Herbal medicine handbook. Lippincott Williams Wilkins A Wolters, Kluwer Co, 2004, pp. 289-290.
  • [14] Bagamboula CF, Uyttendale M, Debevere J. Antimicrobial effect of spicesd and herbs on Shigella sonnei and Shigella flexneri. J Food Prot. 2003; 66: 668-673. https://doi.org/10.4315/0362-028X-66.4.668
  • [15] Velluti A, Sanchis V, Romas AJ, Egido J, Marin S. Inhibitory effect of cinnamon, clove, lemongrass, oregano and palmarose essential oil on growth and fumonisin B1 production by Fusarium proliferatum in maize grain. Int J Food Microbiol. 2003; 89: 145-154. https://doi.org/10.1016/S0168-1605(03)00116-8
  • [16] Nostro A, Blanco AR, Cannatelli MA, Enea V, Flamini G, Morelli I, Sudano RA, Aloazo V. Susceptibility of methicillin resistant Staphylococci to oregano essential oil, carvacrol and thymol. FEMS Microbl Lett. 2004; 230: 191-195. https://doi.org/10.1016/S0378-1097(03)00890-5
  • [17] Van Den Broucke CO, Lemli JA. Antispasmodic activity of Origanum compactum. Planta Med. 1980; 38: 317-331. https://doi.org/10.1055/s-2008-1074884
  • [18] Chung YK, Heo HJ, Kim EK, Huh TL, Lim Y, Kim SK, Shin DH. Inhibitory effect of ursolic acid purified from Origanum majorana L. on the acetylcholinesterase. Mol Cells. 2001; 11: 137-143. https://doi.org/10.1016/S1016-8478(23)17016-6
  • [19] Heo HJ, Cho HY, Hong B, Kim HK, Heo TR, Kim EK, Kim SK, Kim CJ, Shin DH. Ursolic acid of Origanum majorana L. reduces Aâ-induced oxidative injury. Mol Cells. 2002; 13: 5-11. https://doi.org/10.1016/S1016-8478(23)14997-1
  • [20] Lin LT, Liu LT, Chiang LC, Lin CC. In vitro antihepatoma activity of fifteen natural medicines from Canada. Phytother Res. 2002; 16: 440-444. https://doi.org/10.1002/ptr.937
  • [21] Campanella L, Bonanni A, Favero G, Tamassetti M. Determination of antioxidant properties of aromatic herbs, olives and fresh fruit using an enzymatic sensor. Anal Bioanal Chem. 2003; 375: 1011-1016. https://doi.org/10.1007/s00216-003-1825-1
  • [22] Zeytinoglu H, Incesu Z, Baser KHC. Inhibition of DNA synthesis by carvacrol in mouse myoblast cells bearing a human NRAS oncogene. Phytomedicine. 2003; 10: 292-299. https://doi.org/10.1078/094471103322004785
  • [23] Dorman Damien H J, Bachmayer O, Kosar M, Hiltunen R. Antioxidant properties of aqueous extracts from selected Lamiaceae species grown in Turkey. J Agric Food Chem. 2004; 52: 762-770. https://doi.org/10.1021/jf034908v
  • [24] Rostami S. Yaygın veya Endemik Olan Bazı Bitki Türlerinin Antiproliferatif ve Antiinflamatuvar Etkilerinin Araştırılması. Yüksek Lisans Tezi, Ankara: Gazi Üniversitesi Fen Bilimleri Enstitüsü, 2013.
  • [25] Desai AG, Ghulam NQ, Ramesh KG, Mahmoud ET, Jaswant S, Ajit KS, Yashbir S B, Subhash CT, Hari KB. Medicinal plants and cancer chemoprevention. Curr Drug Metab. 2008; 9: 581-591. https://doi.org/10.2174/138920008785821657
  • [26] Ramalingam M, Kim SJ. Insulin on hydrogen peroxide induced oxidative stress involves ROS/Ca2+ and Akt/Bcl-2 signaling pathways. Free Radic Res. 2014; 48: 347-356. https://doi.org/10.3109/10715762.2013.869588
  • [27] Benzi G, Ceci A. Herbal medicines in European regulation. Pharmacol Res. 1997; 35: 355-362. https://doi.org/10.1006/phrs.1997.0132
  • [28] Casio MS, Burotti S, Mannino S, Benedetti S. Use of an electrochemical method of the evaluate the antioxidant activity of herb extracts from the Labiatae family. Food Chem. 2006; 97: 725-731. https://doi.org/10.1016/j.foodchem.2005.05.043
  • [29] Ulubelen A, Öksüz S, Kolak U, Tan N, Johansson CB, Çelik C, Kohlbau HJ, Voelter W. Diterpenoids from roots of Salvia bracteata. Phytochem. 1999; 52: 1455-1459. https://doi.org/10.1016/S0031-9422(99)00453-7
  • [30] Topçu G, Kolak U, Balcı K, Ulubelen A. Structure elucidation of a new rearranged abietane diterpene from a biologically active plant, Salvia eriophora. Nat Prod Comm. 2007; 2: 981-986. https://doi.org/10.1177/1934578X07002010
  • [31] Başer KHC, Kırımer N. Essential oils of Lamiacea plants of Turkey. Acta Hortic. 2006; 723: 163-171. https://doi.org/10.17660/ActaHortic.2006.723.18
  • [32] Gökhan A. Evaluation of cytotoxic, membrane damaging and apoptotic effects of Origanum majorana essential oil on lung cancer and epidermoid carcinoma cells. Cyprus J Med Sci. 2022; 7: 201-206. https://doi.org/10.4274/cjms.2021.2021-142
  • [33] Abdel-Massih RM, Fares R, Bazzi S, El-Chami N, Baydoun E. The apoptotic and anti-proliferative activity of Origanum majorana extracts on human leukemic cell line. Leuk Res. 2010; 34: 1052-1056. https://doi.org/10.1016/j.leukres.2009.09.018
  • [34] Hitoshi T, Yuka K, Takashi G, Kazuo K, Masato N. DPPH radical-scavenging effect of several phenylpropanoid compounds and their glycoside derivatives. Yakugaku Zasshi. 2005; 125: 371–375. https://doi.org/10.1248/yakushi.125.371
  • [35] Hussain AI, Anwar F, Rasheed S, Nigam PS, Janneh O, Sarker SD. Composition, antioxidant and chemotherapeutic properties of the essential oils from two Origanum species growing in Pakistan. Rev Bras Farmacogn. 2011; 21: 943-952. https://doi.org/10.1590/S0102-695X2011005000165
  • [36] Ibrahim EH, Alshahrani MY, Ghramh HA, Alothaid H, Kilany M, Morsy K, El-kott AF, Taha R, El-Mekkawy HI, El-Shaboury GA, El-Mansi AA, Mohammed MEA, Sayed MA, Yahia IS. Origanum majorana harvested from Al-Soda, Saudi Arabia promotes mitotic arrest and apoptosis in colon cancer cells. J King Saud Univ Sci. 2022; 34: 101878. https://doi.org/10.1016/j.jksus.2022.101878
  • [37] Khaleghi S, Khayatzadeh J, Neamati A. Biosynthesis of zinc oxide nanoparticles using Origanum majorana L. leaf extract, its antioxidant and cytotoxic activities. Mater Technol. 2022; 37: 2522-2531. https://doi.org/10.1080/10667857.2022.2044218
  • [38] Al Tamimi NF. Anti-colon cancer activity of Origanum, Thesis, p. 212, United Arab Emirates University, Al Ain, UAE, 2015.
  • [39] Al Dhaheri Y, Eid HA, Arafat K, Iratni R. Origanum majorana extract induces apoptosis and suppresses migration and invasion of MDA-MB-231 Human breast cancer cell line through inactivation of the NFkB pathway. Eur J Cancer. 2012; 48: S233-S234. https://doi.org/10.1016/S0959-8049(12)71589-7
  • [40] Al Dhaheri Y, Eid A, Abuqamar S, Attoub S, Khasawneh M, Aiche G, Hisaindee S, Iratni R. Mitotic arrest and apoptosis in breast cancer cells induced by Origanum majorana extract: Up regulation of TNF-α and down regulation of survivin and mutant p53. PLoS One. 2013; 8: e56649. https://doi.org/10.1371/journal.pone.0056649
  • [41] Aseyd Nezhad S, Es‐haghi A, Tabrizi MH. Green synthesis of cerium oxide nanoparticle using Origanum majorana L. leaf extract, its characterization and biological activities. Appl Organomet Chem. 2020; 34: e5314. https://doi.org/10.1002/aoc.5314
  • [42] Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods. 1983; 65: 55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  • [43] Flohe L, Gunzler WA. Glutathione peroxidase. Methods Enzymol. 1984; 105: 115-121. https://doi.org/10.1016/s0076-6879(84)05015-1
  • [44] Bradford MM. A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem. 1976; 72: 248-254. https://doi.org/10.1016/0003-2697(76)90527-3
  • [45] Kirkman TW. Statistics to Use [Online]. 1996. Retrieved August 17, 2008, from http://www.physics.csbsju.edu/stats/1996
  • [46] Cuendet M, Hostettmann K, Potterat O. Iridoid glucosides with free radical scavenging properties from Fagraea blumei. Helv Chim Acta. 1997; 80: 1144-1152. https://doi.org/10.1002/hlca.19970800411
  • [47] Burits M, Bucar F. Antioxidant activity of Nigella sativa essential oil. Phytother Res. 2000; 14: 323-328. https://doi.org/10.1002/1099-1573(200008)14:5<323::AID-PTR621>3.0.CO;2-Q

Comparison of the cytotoxic effects of different Origanum majorana extracts on lung cancer cells

Yıl 2025, Cilt: 29 Sayı: 2, 507 - 515, 08.04.2025

Ayşe Erdoğan

https://doi.org/10.12991/jrespharm.1660997

Öz

The determination that compounds used for chemotherapeutic purposes in cancer treatment cause cytotoxic effects on healthy cells as well as target cells has led researchers to work on reducing treatment-related toxicity, and accordingly, research on the effectiveness of herbal resources in cancer treatment has gained importance. In our study, ethanol, methanol and chloroform extracts were obtained from the aboveground parts of Origanum majorana L. (O. majorana) and their cytotoxic effects on A-549 cells (non-small cell lung cancer (NSCLC)) were investigated using MTT test. Additionally, the free radical scavenging activities of extracts were demonstrated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) test. It has been shown that cytotoxic effects of extracts increased depending on concentrations on A-549 cells. It was determined that methanol, ethanol and chloroform extracts of O. majorana caused an increase in the activity of lactate dehydrogenase (LDH), and apoptotic enzyme, caspase-3 activity, compared to the control. It has been demonstrated that methanol, ethanol, and chloroform extracts of O. majorana had membrane damaging and apoptotic effects on A-549.

Anahtar Kelimeler

Origanum majorana apoptosis antioxidant DPPH

Kaynakça

  • [1] Greenlee RT, Murray T, Bolden S, Wingpo CA. Cancer statistics. Cancer J Clin. 2000; 50: 7-33. https://doi.org/10.3322/canjclin.50.1.7
  • [2] Roberts AB, Wakefield LM. The two faces of transforming growth factor beta in carcinogenesis. Proc Natl Acad Sci USA. 2003; 100: 8621-8623. https://doi.org/10.1073/pnas.1633291100
  • [3] Parkin DM, Bray F, Ferlay J, Pisani P. Global cancer statistics. CA Cancer J Clin. 2005; 55: 74-108. https://doi.org/10.3322/canjclin.55.2.74
  • [4] Jemal A, Siegel R, Ward E, Hao Y, Xu J, Murray T, Thun MJ. Cancer statistics. CA Cancer J Clin. 2008; 58: 71-96. https://doi.org/10.3322/CA.2007.0010
  • [5] Yurdakul AS, Çalışır HC, Demirağ F, Taci N, Öğretensoy M. The distribution of histological types of lung cancer (Analysis of 2216 cases). Toraks Dergisi. 2002; 3: 59-65.
  • [6] Bunn, PA Jr, Franklin W. Epidermal growth factor receptor expression, signal pathway, and inhibitors in non-small cell lung cancer. Semin Oncol. 2002; 29: 38-44. https://doi.org/10.1053/sonc.2002.35646
  • [7] Travis WD. Pathology of lung cancer. Clin Chest Med. 2011; 32: 669-692. https://doi.org/10.1016/j.ccm.2011.08.005
  • [8] Aygencel ŞG, Oğuzülgen İK, Öztürk C. Predictive and prognostic factors in long term survival of small cell lung cancer. Toraks Dergisi. 2005; 6: 81-84.
  • [9] Yang P, Allen MS, Aubry MC, Wampfler JA, Marks RS, Edell ES, Thibodeau S, Adjei AA, Jett J, Deschamps C. Clinical features of 5,628 primary lung cancer patients: experience at Mayo Clinic from 1997 to 2003. Chest. 2005; 128: 452-462. https://doi.org/10.1378/chest.128.1.452
  • [10] Jeremic B, Milicic B, Dagovic A, Aleksandrovic J, Nikolic N. Pretreatment clinical prognostic factors in patients with stage IV non- small cell lung cancer (NSCLC) treated with chemotherapy. J Cancer Res Clin Oncol. 2003; 129: 114-122. https://doi.org/10.1007/s00432-002-0408-4
  • [11] Vallieres E. Role of adjuvant systemic therapy for stage I NSCLC. Thorac Surg Clin. 2007; 17: 279-285. https://doi.org/10.1016/j.thorsurg.2007.03.001
  • [12] Assaf MH, Ali AA, Makboul MA, Beck JP, Anton R. Preliminary study of phenolic glycerosides from Origanum majorana; quantative estimation of arbutin; cytotoxic activity of hydroquinone. Planta Med. 1987; 53: 343-345. https://doi.org/10.1055/s-2006-962734
  • [13] Kelly WJ. Herbal medicine handbook. Lippincott Williams Wilkins A Wolters, Kluwer Co, 2004, pp. 289-290.
  • [14] Bagamboula CF, Uyttendale M, Debevere J. Antimicrobial effect of spicesd and herbs on Shigella sonnei and Shigella flexneri. J Food Prot. 2003; 66: 668-673. https://doi.org/10.4315/0362-028X-66.4.668
  • [15] Velluti A, Sanchis V, Romas AJ, Egido J, Marin S. Inhibitory effect of cinnamon, clove, lemongrass, oregano and palmarose essential oil on growth and fumonisin B1 production by Fusarium proliferatum in maize grain. Int J Food Microbiol. 2003; 89: 145-154. https://doi.org/10.1016/S0168-1605(03)00116-8
  • [16] Nostro A, Blanco AR, Cannatelli MA, Enea V, Flamini G, Morelli I, Sudano RA, Aloazo V. Susceptibility of methicillin resistant Staphylococci to oregano essential oil, carvacrol and thymol. FEMS Microbl Lett. 2004; 230: 191-195. https://doi.org/10.1016/S0378-1097(03)00890-5
  • [17] Van Den Broucke CO, Lemli JA. Antispasmodic activity of Origanum compactum. Planta Med. 1980; 38: 317-331. https://doi.org/10.1055/s-2008-1074884
  • [18] Chung YK, Heo HJ, Kim EK, Huh TL, Lim Y, Kim SK, Shin DH. Inhibitory effect of ursolic acid purified from Origanum majorana L. on the acetylcholinesterase. Mol Cells. 2001; 11: 137-143. https://doi.org/10.1016/S1016-8478(23)17016-6
  • [19] Heo HJ, Cho HY, Hong B, Kim HK, Heo TR, Kim EK, Kim SK, Kim CJ, Shin DH. Ursolic acid of Origanum majorana L. reduces Aâ-induced oxidative injury. Mol Cells. 2002; 13: 5-11. https://doi.org/10.1016/S1016-8478(23)14997-1
  • [20] Lin LT, Liu LT, Chiang LC, Lin CC. In vitro antihepatoma activity of fifteen natural medicines from Canada. Phytother Res. 2002; 16: 440-444. https://doi.org/10.1002/ptr.937
  • [21] Campanella L, Bonanni A, Favero G, Tamassetti M. Determination of antioxidant properties of aromatic herbs, olives and fresh fruit using an enzymatic sensor. Anal Bioanal Chem. 2003; 375: 1011-1016. https://doi.org/10.1007/s00216-003-1825-1
  • [22] Zeytinoglu H, Incesu Z, Baser KHC. Inhibition of DNA synthesis by carvacrol in mouse myoblast cells bearing a human NRAS oncogene. Phytomedicine. 2003; 10: 292-299. https://doi.org/10.1078/094471103322004785
  • [23] Dorman Damien H J, Bachmayer O, Kosar M, Hiltunen R. Antioxidant properties of aqueous extracts from selected Lamiaceae species grown in Turkey. J Agric Food Chem. 2004; 52: 762-770. https://doi.org/10.1021/jf034908v
  • [24] Rostami S. Yaygın veya Endemik Olan Bazı Bitki Türlerinin Antiproliferatif ve Antiinflamatuvar Etkilerinin Araştırılması. Yüksek Lisans Tezi, Ankara: Gazi Üniversitesi Fen Bilimleri Enstitüsü, 2013.
  • [25] Desai AG, Ghulam NQ, Ramesh KG, Mahmoud ET, Jaswant S, Ajit KS, Yashbir S B, Subhash CT, Hari KB. Medicinal plants and cancer chemoprevention. Curr Drug Metab. 2008; 9: 581-591. https://doi.org/10.2174/138920008785821657
  • [26] Ramalingam M, Kim SJ. Insulin on hydrogen peroxide induced oxidative stress involves ROS/Ca2+ and Akt/Bcl-2 signaling pathways. Free Radic Res. 2014; 48: 347-356. https://doi.org/10.3109/10715762.2013.869588
  • [27] Benzi G, Ceci A. Herbal medicines in European regulation. Pharmacol Res. 1997; 35: 355-362. https://doi.org/10.1006/phrs.1997.0132
  • [28] Casio MS, Burotti S, Mannino S, Benedetti S. Use of an electrochemical method of the evaluate the antioxidant activity of herb extracts from the Labiatae family. Food Chem. 2006; 97: 725-731. https://doi.org/10.1016/j.foodchem.2005.05.043
  • [29] Ulubelen A, Öksüz S, Kolak U, Tan N, Johansson CB, Çelik C, Kohlbau HJ, Voelter W. Diterpenoids from roots of Salvia bracteata. Phytochem. 1999; 52: 1455-1459. https://doi.org/10.1016/S0031-9422(99)00453-7
  • [30] Topçu G, Kolak U, Balcı K, Ulubelen A. Structure elucidation of a new rearranged abietane diterpene from a biologically active plant, Salvia eriophora. Nat Prod Comm. 2007; 2: 981-986. https://doi.org/10.1177/1934578X07002010
  • [31] Başer KHC, Kırımer N. Essential oils of Lamiacea plants of Turkey. Acta Hortic. 2006; 723: 163-171. https://doi.org/10.17660/ActaHortic.2006.723.18
  • [32] Gökhan A. Evaluation of cytotoxic, membrane damaging and apoptotic effects of Origanum majorana essential oil on lung cancer and epidermoid carcinoma cells. Cyprus J Med Sci. 2022; 7: 201-206. https://doi.org/10.4274/cjms.2021.2021-142
  • [33] Abdel-Massih RM, Fares R, Bazzi S, El-Chami N, Baydoun E. The apoptotic and anti-proliferative activity of Origanum majorana extracts on human leukemic cell line. Leuk Res. 2010; 34: 1052-1056. https://doi.org/10.1016/j.leukres.2009.09.018
  • [34] Hitoshi T, Yuka K, Takashi G, Kazuo K, Masato N. DPPH radical-scavenging effect of several phenylpropanoid compounds and their glycoside derivatives. Yakugaku Zasshi. 2005; 125: 371–375. https://doi.org/10.1248/yakushi.125.371
  • [35] Hussain AI, Anwar F, Rasheed S, Nigam PS, Janneh O, Sarker SD. Composition, antioxidant and chemotherapeutic properties of the essential oils from two Origanum species growing in Pakistan. Rev Bras Farmacogn. 2011; 21: 943-952. https://doi.org/10.1590/S0102-695X2011005000165
  • [36] Ibrahim EH, Alshahrani MY, Ghramh HA, Alothaid H, Kilany M, Morsy K, El-kott AF, Taha R, El-Mekkawy HI, El-Shaboury GA, El-Mansi AA, Mohammed MEA, Sayed MA, Yahia IS. Origanum majorana harvested from Al-Soda, Saudi Arabia promotes mitotic arrest and apoptosis in colon cancer cells. J King Saud Univ Sci. 2022; 34: 101878. https://doi.org/10.1016/j.jksus.2022.101878
  • [37] Khaleghi S, Khayatzadeh J, Neamati A. Biosynthesis of zinc oxide nanoparticles using Origanum majorana L. leaf extract, its antioxidant and cytotoxic activities. Mater Technol. 2022; 37: 2522-2531. https://doi.org/10.1080/10667857.2022.2044218
  • [38] Al Tamimi NF. Anti-colon cancer activity of Origanum, Thesis, p. 212, United Arab Emirates University, Al Ain, UAE, 2015.
  • [39] Al Dhaheri Y, Eid HA, Arafat K, Iratni R. Origanum majorana extract induces apoptosis and suppresses migration and invasion of MDA-MB-231 Human breast cancer cell line through inactivation of the NFkB pathway. Eur J Cancer. 2012; 48: S233-S234. https://doi.org/10.1016/S0959-8049(12)71589-7
  • [40] Al Dhaheri Y, Eid A, Abuqamar S, Attoub S, Khasawneh M, Aiche G, Hisaindee S, Iratni R. Mitotic arrest and apoptosis in breast cancer cells induced by Origanum majorana extract: Up regulation of TNF-α and down regulation of survivin and mutant p53. PLoS One. 2013; 8: e56649. https://doi.org/10.1371/journal.pone.0056649
  • [41] Aseyd Nezhad S, Es‐haghi A, Tabrizi MH. Green synthesis of cerium oxide nanoparticle using Origanum majorana L. leaf extract, its characterization and biological activities. Appl Organomet Chem. 2020; 34: e5314. https://doi.org/10.1002/aoc.5314
  • [42] Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods. 1983; 65: 55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  • [43] Flohe L, Gunzler WA. Glutathione peroxidase. Methods Enzymol. 1984; 105: 115-121. https://doi.org/10.1016/s0076-6879(84)05015-1
  • [44] Bradford MM. A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem. 1976; 72: 248-254. https://doi.org/10.1016/0003-2697(76)90527-3
  • [45] Kirkman TW. Statistics to Use [Online]. 1996. Retrieved August 17, 2008, from http://www.physics.csbsju.edu/stats/1996
  • [46] Cuendet M, Hostettmann K, Potterat O. Iridoid glucosides with free radical scavenging properties from Fagraea blumei. Helv Chim Acta. 1997; 80: 1144-1152. https://doi.org/10.1002/hlca.19970800411
  • [47] Burits M, Bucar F. Antioxidant activity of Nigella sativa essential oil. Phytother Res. 2000; 14: 323-328. https://doi.org/10.1002/1099-1573(200008)14:5<323::AID-PTR621>3.0.CO;2-Q
Toplam 47 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Eczacılık ve İlaç Bilimleri (Diğer)
Bölüm Articles
Yazarlar

Ayşe Erdoğan

Yayımlanma Tarihi 8 Nisan 2025
Gönderilme Tarihi 27 Şubat 2024
Kabul Tarihi 22 Mayıs 2024
Yayımlandığı Sayı Yıl 2025 Cilt: 29 Sayı: 2

Kaynak Göster

APA Erdoğan, A. (2025). Comparison of the cytotoxic effects of different Origanum majorana extracts on lung cancer cells. Journal of Research in Pharmacy, 29(2), 507-515. https://doi.org/10.12991/jrespharm.1660997
AMA Erdoğan A. Comparison of the cytotoxic effects of different Origanum majorana extracts on lung cancer cells. J. Res. Pharm. Nisan 2025;29(2):507-515. doi:10.12991/jrespharm.1660997
Chicago Erdoğan, Ayşe. “Comparison of the Cytotoxic Effects of Different Origanum Majorana Extracts on Lung Cancer Cells”. Journal of Research in Pharmacy 29, sy. 2 (Nisan 2025): 507-15. https://doi.org/10.12991/jrespharm.1660997.
EndNote Erdoğan A (01 Nisan 2025) Comparison of the cytotoxic effects of different Origanum majorana extracts on lung cancer cells. Journal of Research in Pharmacy 29 2 507–515.
IEEE A. Erdoğan, “Comparison of the cytotoxic effects of different Origanum majorana extracts on lung cancer cells”, J. Res. Pharm., c. 29, sy. 2, ss. 507–515, 2025, doi: 10.12991/jrespharm.1660997.
ISNAD Erdoğan, Ayşe. “Comparison of the Cytotoxic Effects of Different Origanum Majorana Extracts on Lung Cancer Cells”. Journal of Research in Pharmacy 29/2 (Nisan 2025), 507-515. https://doi.org/10.12991/jrespharm.1660997.
JAMA Erdoğan A. Comparison of the cytotoxic effects of different Origanum majorana extracts on lung cancer cells. J. Res. Pharm. 2025;29:507–515.
MLA Erdoğan, Ayşe. “Comparison of the Cytotoxic Effects of Different Origanum Majorana Extracts on Lung Cancer Cells”. Journal of Research in Pharmacy, c. 29, sy. 2, 2025, ss. 507-15, doi:10.12991/jrespharm.1660997.
Vancouver Erdoğan A. Comparison of the cytotoxic effects of different Origanum majorana extracts on lung cancer cells. J. Res. Pharm. 2025;29(2):507-15.