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Yıl 2025, Cilt: 29 Sayı: 4, 1627 - 1635, 05.07.2025

Pervin Rayaman , Yusuf Talha Atalay

https://doi.org/10.12991/jrespharm.1680880

Öz

Proje Numarası

2209-A University Students Research Projects Support Program: 1919B012318721

Kaynakça

  • [1] FAO/WHO (2001). Health and Nutritional Properties of Probiotics in Food Including Powder Milk with Live Lactic Acid Bacteria—Report of a Joint FAO/WHO Expert Consultation on Evaluation of Health and Nutritional Properties of Probiotics in Food Including Power Milk with Liver Lactic Acid Bacteria. https://www.iqb.es/digestivo/pdfs/probioticos.pdf (accessed on 22 December 2024).
  • [2] Hill C, Guarner F, Reid G, Gibson GR, Merenstein DJ, Pot B, Morelli L, Canani RB, Flint HJ, Salminen S, Calder PC, Sanders ME. Expert consensus document. The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nat Rev Gastroenterol Hepatol. 2014;11(8):506-514. https://doi.org/10.1038/nrgastro.2014.66
  • [3] Dogan M. Élie Metchnikoff (1845- 1916) Pioneer of Probiotic Foods. 2024;2024.
  • [4] Altuntaş Y, Batman A Microbiota and metabolic syndrome. Turk Kardiyol Dern Ars. 2017;45(3):286-296. https://doi.org/10.5543/tkda.2016.72461.
  • [5] Asghari Ozma M, Mahmoodzadeh Hosseini H, Ataee MH, Mirhosseini SA. Evaluating the antibacterial, antibiofilm, and anti-toxigenic effects of postbiotics from lactic acid bacteria on Clostridium difficile. Iran J Microbiol. 2024;16(4):497-508. https://doi.org/10.18502/ijm.v16i4.16309
  • [6] Rahman MN, Barua N, Tin MCF, Dharmaratne P, Wong SH, Ip M. The use of probiotics and prebiotics in decolonizing pathogenic bacteria from the gut; a systematic review and meta-analysis of clinical outcomes. Gut Microbes. 2024;16(1):2356279. https://doi.org/10.1080/19490976.2024.2356279
  • [7] Lungaro L, Malfa P, Manza F, Costanzini A, Valentini G, Squarzanti DF, Viciani E, Velichevskaya A, Castagnetti A, Barbalinardo M, Gentili D, Cariani A, Ghisellini S, Caputo F, De Giorgio R, Caio G. Clinical Efficacy of Probiotics for Allergic Rhinitis: Results of an Exploratory Randomized Controlled Trial. Nutrients. 2024; 16(23):4173. https://doi.org/10.3390/nu16234173
  • [8] Zhu F, Yin S, Wang Y, Zhong Y, Ji Q, Wu J. Effects of Probiotics on Neurodegenerative Disease-Related Symptoms and Systemic Inflammation: A Systematic Review. Int J Gen Med. 2024;17:5941-5958. https://doi.org/10.2147/IJGM.S499406
  • [9] Anumudu CK, Miri T, Onyeaka H. Multifunctional Applications of Lactic Acid Bacteria: Enhancing Safety, Quality, and Nutritional Value in Foods and Fermented Beverages. Foods. 2024; 13(23):3714. https://doi.org/10.3390/foods13233714
  • [10] Linares DM, Gómez C, Renes E, Fresno JM, Tornadijo ME, Ross RP, Stanton C. Lactic Acid Bacteria and Bifidobacteria with Potential to Design Natural Biofunctional Health-Promoting Dairy Foods. Front Microbiol. 2017;8:846. https://doi.org/10.3389/fmicb.2017.00846
  • [11] Bi Y, Liang L, Qiao K, Luo J, Liu X, Sun B, Zhang Y. A comprehensive review of plant-derived salt substitutes: Classification, mechanism, and application. Food Res Int. 2024;194:114880. https://doi.org/10.1016/j.foodres.2024.114880
  • [12] World Health Organization WHO (2023). Salt reduction. https://www.who.int/zh/news/item/09-03-2023-massive-efforts-needed-to-reduce-salt-intake-and-protectlives (Accessed on 23 April 2024).
  • [13] Wang X, Lang F, Liu D. High-Salt Diet and Intestinal Microbiota: Influence on Cardiovascular Disease and Inflammatory Bowel Disease. Biology. 2024; 13(9):674. https://doi.org/10.3390/biology13090674
  • [14] Marklund M, Trieu K, Aminde LN, Cobiac L, Coyle DH, Huang L, Neal B, Veerman L, Wu JHY. Estimated health effect, cost, and cost-effectiveness of mandating sodium benchmarks in Australia's packaged foods: a modelling study. Lancet Public Health. 2024;9(11):e861-e870. https://doi.org/10.1016/S2468-2667(24)00219-6
  • [15] Tetik N, Rayaman P, Rayaman E, Adaleti R. Antimicrobial effect of probiotic microorganisms on clinical and standard Staphylococcus aureus isolates. J Res Pharm. 2023; 27(6): 2374-2388. http://dx.doi.org/10.29228/jrp.525
  • [16] Nath S, Sikidar J, Roy M, Deb B. In vitro screening of probiotic properties of Lactobacillus plantarum isolated from fermented milk product. Food Quality and Safety. 2020;4(4):213-223. https://doi.org/10.1093/fqsafe/fyaa026.
  • [17] Mulaw G, Sisay Tessema T, Muleta D, Tesfaye A. In Vitro Evaluation of Probiotic Properties of Lactic Acid Bacteria Isolated from Some Traditionally Fermented Ethiopian Food Products [published correction appears in Int J Microbiol. 2020 Jul 24;2020:6401356. doi: 10.1155/2020/6401356.]. Int J Microbiol. 2019;2019:7179514. https://doi.org/10.1155/2019/7179514
  • [18] Rocha-Ramírez LM, Hernández-Chiñas U, Moreno-Guerrero SS, Ramírez-Pacheco A, Eslava CA. Probiotic Properties and Immunomodulatory Activity of Lactobacillus Strains Isolated from Dairy Products. Microorganisms. 2021;9(4):825. https://doi.org/10.3390/microorganisms9040825
  • [19] El-Sayed AIM, El-Borai AM, Akl SH, El-Aassar SA, Abdel-Latif MS. Identification of Lactobacillus strains from human mother milk and cottage cheese revealed potential probiotic properties with enzymatic activity. Sci Rep. 2022;12(1):22522. https://doi.org/10.1038/s41598-022-27003-2
  • [20] Halder D, Mandal M, Chatterjee SS, Pal NK, Mandal S. Indigenous Probiotic Lactobacillus Isolates Presenting Antibiotic like Activity against Human Pathogenic Bacteria. Biomedicines. 2017;5(2):31. https://doi.org/10.3390/biomedicines5020031
  • [21] Betancur C, Martínez Y, Tellez-Isaias G, Avellaneda MC, Velázquez-Martí B. In Vitro Characterization of Indigenous Probiotic Strains Isolated from Colombian Creole Pigs. Animals (Basel). 2020;10(7):1204. https://doi.org/10.3390/ani10071204
  • [22] Luo X, Li M, Zhang H, Yan D, Ji S, Wu R, Chen Y. Gene expression of Lactobacillus plantarum FS5-5 in response to salt stress. Ann Microbiol. 2016;66(3):1181–1188. https://doi:10.1007/s13213-016-1199-1
  • [23] Tunalı Y. Farmasötik mikrobiyoloji uygulamaları. 2nd ed. Istanbul, Turkey: Star Ajans Ltd. Şti; 2014.
  • [24] Harrigan WF, McCance ME. Laboratory methods in Mikrobiology. New York: Academik Press; 1966, p: 8-65.
  • [25] Jorgensen JH, Pfaller MA, Carroll KC, Funke G, eds. Manual of Clinical Microbiology. 11th ed. American Society for Microbiology; 2016. Chapter 3.17.1. https://doi.org/10.1128/9781555818814.ch3.17.1.
  • [26] Facklam RR, Sahm DS, Teixeira LM. Standard laboratory methods for identifying and growing enterococci. In: Murray PR, Baron EJ, Jorgensen JH, Landry ML, Pfaller MA, eds. Manual of Clinical Microbiology. 8th ed. Washington, DC: ASM Press; 2002:2142-2152.
  • [27] Champagne CP, Raymond Y, Tompkins TA. The determination of viable counts in probiotic cultures microencapsulated by spray-coating. Food Microbiol. 2010;27(8):1104-1111. https://doi.org/10.1016/j.fm.2010.07.017
  • [28] Champagne CP, Ross RP, Saarela M, Hansen KF, Charalampopoulos D. Recommendations for the viability assessment of probiotics as concentrated cultures and in food matrices. Int J Food Microbiol. 2011;149(3):185-193. https://doi.org/10.1016/j.ijfoodmicro.2011.07.005
  • [29] Council of Europe. 2019. “Microbiological examination of non-sterile products, microbial enumeration tests”. European Pharmacopoeia. Council of Europe. Strasbourg Cedex, France. https://ehpm.org/wp-content/uploads/2022/04/QG22_2-6-12_Microbiological_examination_of_non-sterile_products-microbial_enumeration_tests_ep10-2.pdf (Accessed on 22 December 2024).
  • [30] Smith and Hussey (2005). Gram Stain Protocols. https://asm.org/getattachment/5c95a063-326b-4b2f-98ce-001de9a5ece3/gram-stain-protocol-2886.pdf (Accessed on 7 April 2025).

Tolerance of Probiotic Microorganisms to Sodium Chloride

Yıl 2025, Cilt: 29 Sayı: 4, 1627 - 1635, 05.07.2025

Pervin Rayaman , Yusuf Talha Atalay

https://doi.org/10.12991/jrespharm.1680880

Öz

Since the increasing consumption of packaged and ready-to-eat foods leads to excessive sodium intake, this study aims to investigate the effects of various sodium concentrations on probiotic microorganisms, which are key members of the human microbiota. A total of 5 probiotic microorganisms (3 different Lactobacillus plantarum (L. plantarum) strains, Lactobacillus kefiri (L. kefiri), Lactobacillus acidophilus (L. acidophilus)) were used in the present study. In order to find out the impact of varying concentrations of sodium chloride (1%, 2%, 6%, and 10%) on probiotic microorganisms viability, the formed colonies on de Man Ragosa Sharp (MRS) agar were assessed by plate count. Bacterial growth curves were prepared according to the visible colonies on the plates. It was found that, Lactobacillus acidophilus ATCC 4356 demonstrated significantly higher tolerance to sodium, particularly at the concentrations of 6% and 10%. In contrast, all of the L. plantarum strains exhibited limited tolerance at higher concentrations. The survival rate of L. acidophilus and L. kefiri at high concentrations of sodium for extend time is promising in order to develop new probiotic preparations in the pharmaceutical industry.

Anahtar Kelimeler

Probiotics Sodium chloride Lactobacillus Packaged products

Destekleyen Kurum

TUBITAK

Proje Numarası

2209-A University Students Research Projects Support Program: 1919B012318721

Teşekkür

The authors appreciated to Asst. Prof. Dr. Erkan Rayaman for his contribution in this research.

Kaynakça

  • [1] FAO/WHO (2001). Health and Nutritional Properties of Probiotics in Food Including Powder Milk with Live Lactic Acid Bacteria—Report of a Joint FAO/WHO Expert Consultation on Evaluation of Health and Nutritional Properties of Probiotics in Food Including Power Milk with Liver Lactic Acid Bacteria. https://www.iqb.es/digestivo/pdfs/probioticos.pdf (accessed on 22 December 2024).
  • [2] Hill C, Guarner F, Reid G, Gibson GR, Merenstein DJ, Pot B, Morelli L, Canani RB, Flint HJ, Salminen S, Calder PC, Sanders ME. Expert consensus document. The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nat Rev Gastroenterol Hepatol. 2014;11(8):506-514. https://doi.org/10.1038/nrgastro.2014.66
  • [3] Dogan M. Élie Metchnikoff (1845- 1916) Pioneer of Probiotic Foods. 2024;2024.
  • [4] Altuntaş Y, Batman A Microbiota and metabolic syndrome. Turk Kardiyol Dern Ars. 2017;45(3):286-296. https://doi.org/10.5543/tkda.2016.72461.
  • [5] Asghari Ozma M, Mahmoodzadeh Hosseini H, Ataee MH, Mirhosseini SA. Evaluating the antibacterial, antibiofilm, and anti-toxigenic effects of postbiotics from lactic acid bacteria on Clostridium difficile. Iran J Microbiol. 2024;16(4):497-508. https://doi.org/10.18502/ijm.v16i4.16309
  • [6] Rahman MN, Barua N, Tin MCF, Dharmaratne P, Wong SH, Ip M. The use of probiotics and prebiotics in decolonizing pathogenic bacteria from the gut; a systematic review and meta-analysis of clinical outcomes. Gut Microbes. 2024;16(1):2356279. https://doi.org/10.1080/19490976.2024.2356279
  • [7] Lungaro L, Malfa P, Manza F, Costanzini A, Valentini G, Squarzanti DF, Viciani E, Velichevskaya A, Castagnetti A, Barbalinardo M, Gentili D, Cariani A, Ghisellini S, Caputo F, De Giorgio R, Caio G. Clinical Efficacy of Probiotics for Allergic Rhinitis: Results of an Exploratory Randomized Controlled Trial. Nutrients. 2024; 16(23):4173. https://doi.org/10.3390/nu16234173
  • [8] Zhu F, Yin S, Wang Y, Zhong Y, Ji Q, Wu J. Effects of Probiotics on Neurodegenerative Disease-Related Symptoms and Systemic Inflammation: A Systematic Review. Int J Gen Med. 2024;17:5941-5958. https://doi.org/10.2147/IJGM.S499406
  • [9] Anumudu CK, Miri T, Onyeaka H. Multifunctional Applications of Lactic Acid Bacteria: Enhancing Safety, Quality, and Nutritional Value in Foods and Fermented Beverages. Foods. 2024; 13(23):3714. https://doi.org/10.3390/foods13233714
  • [10] Linares DM, Gómez C, Renes E, Fresno JM, Tornadijo ME, Ross RP, Stanton C. Lactic Acid Bacteria and Bifidobacteria with Potential to Design Natural Biofunctional Health-Promoting Dairy Foods. Front Microbiol. 2017;8:846. https://doi.org/10.3389/fmicb.2017.00846
  • [11] Bi Y, Liang L, Qiao K, Luo J, Liu X, Sun B, Zhang Y. A comprehensive review of plant-derived salt substitutes: Classification, mechanism, and application. Food Res Int. 2024;194:114880. https://doi.org/10.1016/j.foodres.2024.114880
  • [12] World Health Organization WHO (2023). Salt reduction. https://www.who.int/zh/news/item/09-03-2023-massive-efforts-needed-to-reduce-salt-intake-and-protectlives (Accessed on 23 April 2024).
  • [13] Wang X, Lang F, Liu D. High-Salt Diet and Intestinal Microbiota: Influence on Cardiovascular Disease and Inflammatory Bowel Disease. Biology. 2024; 13(9):674. https://doi.org/10.3390/biology13090674
  • [14] Marklund M, Trieu K, Aminde LN, Cobiac L, Coyle DH, Huang L, Neal B, Veerman L, Wu JHY. Estimated health effect, cost, and cost-effectiveness of mandating sodium benchmarks in Australia's packaged foods: a modelling study. Lancet Public Health. 2024;9(11):e861-e870. https://doi.org/10.1016/S2468-2667(24)00219-6
  • [15] Tetik N, Rayaman P, Rayaman E, Adaleti R. Antimicrobial effect of probiotic microorganisms on clinical and standard Staphylococcus aureus isolates. J Res Pharm. 2023; 27(6): 2374-2388. http://dx.doi.org/10.29228/jrp.525
  • [16] Nath S, Sikidar J, Roy M, Deb B. In vitro screening of probiotic properties of Lactobacillus plantarum isolated from fermented milk product. Food Quality and Safety. 2020;4(4):213-223. https://doi.org/10.1093/fqsafe/fyaa026.
  • [17] Mulaw G, Sisay Tessema T, Muleta D, Tesfaye A. In Vitro Evaluation of Probiotic Properties of Lactic Acid Bacteria Isolated from Some Traditionally Fermented Ethiopian Food Products [published correction appears in Int J Microbiol. 2020 Jul 24;2020:6401356. doi: 10.1155/2020/6401356.]. Int J Microbiol. 2019;2019:7179514. https://doi.org/10.1155/2019/7179514
  • [18] Rocha-Ramírez LM, Hernández-Chiñas U, Moreno-Guerrero SS, Ramírez-Pacheco A, Eslava CA. Probiotic Properties and Immunomodulatory Activity of Lactobacillus Strains Isolated from Dairy Products. Microorganisms. 2021;9(4):825. https://doi.org/10.3390/microorganisms9040825
  • [19] El-Sayed AIM, El-Borai AM, Akl SH, El-Aassar SA, Abdel-Latif MS. Identification of Lactobacillus strains from human mother milk and cottage cheese revealed potential probiotic properties with enzymatic activity. Sci Rep. 2022;12(1):22522. https://doi.org/10.1038/s41598-022-27003-2
  • [20] Halder D, Mandal M, Chatterjee SS, Pal NK, Mandal S. Indigenous Probiotic Lactobacillus Isolates Presenting Antibiotic like Activity against Human Pathogenic Bacteria. Biomedicines. 2017;5(2):31. https://doi.org/10.3390/biomedicines5020031
  • [21] Betancur C, Martínez Y, Tellez-Isaias G, Avellaneda MC, Velázquez-Martí B. In Vitro Characterization of Indigenous Probiotic Strains Isolated from Colombian Creole Pigs. Animals (Basel). 2020;10(7):1204. https://doi.org/10.3390/ani10071204
  • [22] Luo X, Li M, Zhang H, Yan D, Ji S, Wu R, Chen Y. Gene expression of Lactobacillus plantarum FS5-5 in response to salt stress. Ann Microbiol. 2016;66(3):1181–1188. https://doi:10.1007/s13213-016-1199-1
  • [23] Tunalı Y. Farmasötik mikrobiyoloji uygulamaları. 2nd ed. Istanbul, Turkey: Star Ajans Ltd. Şti; 2014.
  • [24] Harrigan WF, McCance ME. Laboratory methods in Mikrobiology. New York: Academik Press; 1966, p: 8-65.
  • [25] Jorgensen JH, Pfaller MA, Carroll KC, Funke G, eds. Manual of Clinical Microbiology. 11th ed. American Society for Microbiology; 2016. Chapter 3.17.1. https://doi.org/10.1128/9781555818814.ch3.17.1.
  • [26] Facklam RR, Sahm DS, Teixeira LM. Standard laboratory methods for identifying and growing enterococci. In: Murray PR, Baron EJ, Jorgensen JH, Landry ML, Pfaller MA, eds. Manual of Clinical Microbiology. 8th ed. Washington, DC: ASM Press; 2002:2142-2152.
  • [27] Champagne CP, Raymond Y, Tompkins TA. The determination of viable counts in probiotic cultures microencapsulated by spray-coating. Food Microbiol. 2010;27(8):1104-1111. https://doi.org/10.1016/j.fm.2010.07.017
  • [28] Champagne CP, Ross RP, Saarela M, Hansen KF, Charalampopoulos D. Recommendations for the viability assessment of probiotics as concentrated cultures and in food matrices. Int J Food Microbiol. 2011;149(3):185-193. https://doi.org/10.1016/j.ijfoodmicro.2011.07.005
  • [29] Council of Europe. 2019. “Microbiological examination of non-sterile products, microbial enumeration tests”. European Pharmacopoeia. Council of Europe. Strasbourg Cedex, France. https://ehpm.org/wp-content/uploads/2022/04/QG22_2-6-12_Microbiological_examination_of_non-sterile_products-microbial_enumeration_tests_ep10-2.pdf (Accessed on 22 December 2024).
  • [30] Smith and Hussey (2005). Gram Stain Protocols. https://asm.org/getattachment/5c95a063-326b-4b2f-98ce-001de9a5ece3/gram-stain-protocol-2886.pdf (Accessed on 7 April 2025).
Toplam 30 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Farmasotik Mikrobiyoloji
Bölüm Articles
Yazarlar

Pervin Rayaman

Yusuf Talha Atalay 0009-0008-6646-6838

Proje Numarası 2209-A University Students Research Projects Support Program: 1919B012318721
Yayımlanma Tarihi 5 Temmuz 2025
Gönderilme Tarihi 21 Nisan 2025
Kabul Tarihi 16 Mayıs 2025
Yayımlandığı Sayı Yıl 2025 Cilt: 29 Sayı: 4

Kaynak Göster

APA Rayaman, P., & Atalay, Y. T. (2025). Tolerance of Probiotic Microorganisms to Sodium Chloride. Journal of Research in Pharmacy, 29(4), 1627-1635. https://doi.org/10.12991/jrespharm.1680880
AMA Rayaman P, Atalay YT. Tolerance of Probiotic Microorganisms to Sodium Chloride. J. Res. Pharm. Temmuz 2025;29(4):1627-1635. doi:10.12991/jrespharm.1680880
Chicago Rayaman, Pervin, ve Yusuf Talha Atalay. “Tolerance of Probiotic Microorganisms to Sodium Chloride”. Journal of Research in Pharmacy 29, sy. 4 (Temmuz 2025): 1627-35. https://doi.org/10.12991/jrespharm.1680880.
EndNote Rayaman P, Atalay YT (01 Temmuz 2025) Tolerance of Probiotic Microorganisms to Sodium Chloride. Journal of Research in Pharmacy 29 4 1627–1635.
IEEE P. Rayaman ve Y. T. Atalay, “Tolerance of Probiotic Microorganisms to Sodium Chloride”, J. Res. Pharm., c. 29, sy. 4, ss. 1627–1635, 2025, doi: 10.12991/jrespharm.1680880.
ISNAD Rayaman, Pervin - Atalay, Yusuf Talha. “Tolerance of Probiotic Microorganisms to Sodium Chloride”. Journal of Research in Pharmacy 29/4 (Temmuz 2025), 1627-1635. https://doi.org/10.12991/jrespharm.1680880.
JAMA Rayaman P, Atalay YT. Tolerance of Probiotic Microorganisms to Sodium Chloride. J. Res. Pharm. 2025;29:1627–1635.
MLA Rayaman, Pervin ve Yusuf Talha Atalay. “Tolerance of Probiotic Microorganisms to Sodium Chloride”. Journal of Research in Pharmacy, c. 29, sy. 4, 2025, ss. 1627-35, doi:10.12991/jrespharm.1680880.
Vancouver Rayaman P, Atalay YT. Tolerance of Probiotic Microorganisms to Sodium Chloride. J. Res. Pharm. 2025;29(4):1627-35.