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Yıl 2025, Cilt: 29 Sayı: 4, 1367 - 1373, 05.07.2025

John Paul Sese Tosoc Mylene Mondarte Uy Wtpsk Senarath Olga Macas Nuñeza

https://doi.org/10.12991/jrespharm.1734445

Öz

Kaynakça

  • [1] Galvez JZ. The need for national colloquium on medicinal plants research and business opportunities: Proceedings of the seminar on the State of the Art of Medicinal Plant Research and Business Opportunities. Manila, Philippines; 2003.
  • [2] The Tboli Tribe of South Cotabato. Philippines, Mindanao, T’boli woman girls. http://everywheremag.com/articles/1178 (accessed on 15 July 2016).
  • [3] Baay JM, Buhian SD, Diocales AT. BS Thesis. Bioactivity of commonly used medicinal plants by T’bolis of South Cotabato. College of Arts and Sciences, Notre Dame of Dadiangas University, General Santos City, Philippines, 2013.
  • [4] Talavera J, Manalo F, Baybay A, Saludario D, Dizon R, Mauro B, Porquerino A, Novela A, Yakit F, Banares A, Franscisco M, Inocencio R, Rongavilla C, Cruz T. The T’boli: Songs, Stories, and Society. A descriptive study on the T’boli language, lifestyle, marriage, political system, and religion; 2013. https://www.researchgate.net/publication/304347631_The_T'boli_Songs_Stories_and _Society (accessed on 15 July 2024).
  • [5] List of Philippine Medicinal Plants with Chinese Names. http://www.stuartxchange.com/Dadayem.html (accessed on 18 March 2024).
  • [6] Karis PO, Ryding O. Asteraceae Cladistics and Classification, Bremer K. Eds, 559–569, Timber Press, Portland, Oregon, USA 1994.
  • [7] Odhay B, Beekrum S, Akula U, Baijnath H. Preliminary assessment of nutritional value of traditional leafy vegetables in Kwazulu-Natal, South Africa. J Food Compost Anal. 2007; (20): 430-435. https://doi.org/10.1016/j.jfca.2006.04.015
  • [8] Pozharitskaya ON, Shikov AN, Makarova MN, Kosman VM, Faustova NM, Tesakova SV, Makarov VG, Galambosi B. Anti-inflammatory activity of a HPLC-fingerprinted aqueous infusion of aerial part of Bidens tripartita L. Phytomedicine. 2010;17(6):463-468. https://doi.org/10.1016/j.phymed.2009.08.001.
  • [9] Vineela CH, Elizabeth KM. Antimicrobial activity of marine algae of Visakhapatnam City, Andhra Pradesh. Asian J Microbiol Biotechnol Environ Sci. 2005; 7: 209-212.
  • [10] Ekpo MA, Etim PC. Antimicrobial activity of ethanolic and aqueous extracts of Sida acuta on microorganisms from skin infections. J Med Plant Res. 2009; 3(9): 621-624.
  • [11] Tosoc JPS, Frediles VCP, Canda C, Demayo CD. Antiangiogenic, antitoxic and antioxidant properties of methanolic extracts of Caladium bicolor (Aiton) Venten. Hum Vet Med. 2016; 8(1): 10-16.
  • [12] Sofowa A. Medicinal Plants and Traditional Medicine in Africa. 1st ed., New York: John Wiley and Sons Ltd. Hoboken, NJ, USA 1982, pp. 168-171.
  • [13] Arora DS, Kaur J. Antimicrobial activity of spices. Int J Antimicrob Agents. 1999; 12(3): 257-262. https://doi.org/10.1016/S0924-8579(99)00074-6
  • [14] Ahmedulla M, Nayar MP. Red data book of Indian plants: Calcutta: Botanical Survey of India, New Delhi, India 1999.
  • [15] Rios J, Recio M. Medicinal plants, and antimicrobial activity. J Ethnopharmacol. 2005; 100: 80-84. https://doi.org/10.1016/j.jep.2005.04.025
  • [16] Nweze EL, Okafor JI, Njokn O. Antimicrobial activities of methanolic extracts of Trema guinensis (Schumm and Thorn) and Morinda Lucida Benth used in Nigeria. Biology Res. 2004; 2: 39-46. https://doi.org/10.4314/br.v2i1.28540
  • [17] Ashis G. Herbal folk remedies of Bankura and Medinipur districts, West Bengal. Indian Journal of Traditional Knowledge, 2003; 2: 393-396.
  • [18] Adegbite AE, Sanyaolu EB. Cytotoxicity testing of aqueous extracts of bitter leaf (Vernonia amugdalina Del.) using the Allium cepa chromosome aberration assay. Sci Res Essay. 2009; 4(11): 1311-1314. https://doi.org/10.4314/ahs.v17i1.19
  • [19] Dahlberg A, Trygger S. Indigenous medicine and primary health care: The importance of lay knowledge and use of medicinal plants in rural South Africa. Hum Ecol. 2009; 37(1): 79-94. https://dx.doi.org/10.1007/s10745-009-9217-6
  • [20] Uprety Y, Asselin H, Boon E, Yadav S, Shrestha K. Indigenous use and bio-efficacy of medicinal plants in the Rasuwa District, Central Nepal. J Ethnobiol Ethnomed. 2010; 6:3. https://doi.org/10.1186/1746-4269-6-3
  • [21] Romeiras M, Duarte MC, Indjai B, Catarino L. Medicinal plants used to treat neurological disorders in West Africa: A case study with Guinea-Bissau Flora. Am J Plant Sci. 2012; 3(7): 1028-1036. https://doi.org/10.4236/ajps.2012.327122
  • [22] Hossan S, Hanif A, Agarwala B, Sarwar S, Karim M. Traditional use of medicinal plants in Bangladesh to treat urinary tract infections and sexually transmitted diseases. Ethnobot Res App. 2010; 8: 61-74.
  • [23] Yang HL, Chen SC, Chang NW, Chang JM, Lee ML, Tsai PC, Fu HH, Kao WW, Chiang HC, Wang HH, Hseu YC. Protection from oxidative damage using Bidens pilosa extracts in normal human erythrocytes. Food Chem Toxicol. 2006;44(9):1513-1521. https://doi.org/10.1016/j.fct.2006.04.006
  • [24] Devi KP, Suganthy N, Kesika P, Pandian SK. Bioprotective properties of seaweeds: In vitro evaluation of antioxidant activity and antimicrobial activity against food-borne bacteria in relation to polyphenolic content. BMC Complement Altern Med. 2008; 8: 38. https://doi.org/10.1186/1472-6882-8-38
  • [25] Djacbou DS, Anatole PC, Cabral BP, Veronique PB. Comparison of in vitro antioxidant properties of extracts from three plants used for medical purpose in Cameroon: Acalypha racemosa, Garcinia lucida and Hymenocardia lyra. Asian Pac J Trop Biomed. 2014; 4: S625–S632. https://doi.org/10.12980/APJTB.4.201414B168
  • [26] Murillo-Alvarez JI, Encarnacion DR, Franzblau SG. Antimicrobial and cytotoxic activity of some medicinal plants from Baja California Sur (Mexico). Pharm Biol. 2001; 39: 6, 445-449. https://doi.org/10.1076/phbi.39.6.445.5877
  • [27] Deng SX, West BJ, Jensen CJ. A quantitative comparison of phytochemical components in global noni fruits and their commercial products. Food Chem. 2010; 122(1): 267-270. https://doi.org/10.1016/j.foodchem.2010.01.031.
  • [28] Ramakrishna A, Ravishankar GA. Influence of abiotic stress signals on secondary metabolites in plants. Plant Signal Behav. 2011; 6(11): 1720-1731. https://doi.org/10.4161/psb.6.11.17613
  • [29] Pavarini DP, Pavarini SP, Niehues M, Lopes NP. Exogenous influences on plant secondary metabolite levels. Anim Feed Sci Technol. 2012; 176(1–4): 5-16. https://doi.org/10.1016/j.anifeedsci.2012.07.002
  • [30] Chang JS, Chiang LC, Chen CC, Liu LT, Wang KC, Lin CC. Antileukemic activity of Bidens pilosa L. var. minor (Blume) Sherff and Houttuynia cordata Thunb. Am J Chin Med. 2001; 29: 303–312. https://doi.org/10.1142/S0192415X01000320
  • [31] Wu LW, Chiang YM, Chuang HC, Wang SY, Yang GW, Chen YH, Lai LY, Shyur LF. Polyacetylenes function as antiangiogenic agents. Pharm Res. 2004; 21: 2112–2119. https://doi.org/10.1023/b:pham.0000048204.08865.41
  • [32] Aguinaldo AM, Espeso EI, Guevara BQ, Nonato MG. Phytochemistry. In: Guevara, B.Q. (Ed.), A Guidebook to Plant Screening: Phytochemical and Biological. University of Santo Tomas, Manila, Philippines 2005.
  • [33] De Padua LS, Lugod GC, Pancho JV. Handbook of Philippine Medicinal Plants. University of the Philippines, Los Baños 1997.
  • [34] Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem. 1999; 269(2): 337–341. https://doi.org/10.1006/abio.1999.4019
  • [35] Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966; 45(4): 493-496. https://doi.org/10.1093/ajcp/45.4_ts.493
  • [36] Bonev B, Hooper J, Parisot J. Principles of assessing bacterial susceptibility to antibiotics using the agar diffusion method. J Antimicrob Chemother. 2008; 61(6): 1295- 301. https://doi.org/10.1093/jac/dkn090.
  • [37] Meyer BN, Ferrigini NR, Putnam JE, Jacobson LB, Nichols DE, McLaughlin JL. Brine Shrimp: A convenient general bioassay for active plant constituents. Planta Med. 1982; 45: 31-34. https://doi.org/10.1055/s-2007-971236
  • [38] Silva LAB, Azevedo LU, Consolaro A, Barnett F, Xu Y, Battaglino RA, Cañadas PS, de Oliveira KMH, Silva RAB. Novel endodontic sealers induce cell cytotoxicity and apoptosis in a dose-dependent behavior and favorable response in mice subcutaneous tissue. Clin Oral Invest. 2017; 21(9): 2851-2861. https://doi.org/ 10.1007/s00784-017-2087-1
  • [39] Mahmoodinia Maymand M, Soleimanpour-lichaei HR, Ardeshirylajimi A, Soleimani M, Enderami SE, Nojehdehi S, Behjati F, Kabir Salmani M. Improvement of hepatogenic differentiation of iPS cells on an aligned polyethersulfone compared to random nanofibers. Artif Cells Nanomed Biotechnol. 2018; 46(4): 853-860. https://doi.org/10.1080/21691401.2017.1345929

Phytochemical screening, antioxidant, antimicrobial, and cytotoxicity evaluation of the leaf extracts of Bidens pilosa by the T'boli tribe in South Cotabato, Philippines

Yıl 2025, Cilt: 29 Sayı: 4, 1367 - 1373, 05.07.2025

John Paul Sese Tosoc Mylene Mondarte Uy Wtpsk Senarath Olga Macas Nuñeza

https://doi.org/10.12991/jrespharm.1734445

Öz

This study determined the bioactive compounds and evaluated the antioxidant, antimicrobial, and cytotoxicity activities of Bidens pilosa aqueous and ethanolic extracts against bacterial and fungal pathogens and various human cancer cell lines. The B. pilosa leaves were extracted using ethanol and water as solvents. The aqueous (BA) and ethanolic (BE) extracts of B. pilosa were subjected to qualitative phytochemical screening. The phosphomolybdate method was used to determine the total antioxidant activity of the extracts. The antimicrobial activity of the extracts against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, and Candida tropicalis was determined using the Kirby-Bauer method. Lastly, the brine shrimp lethality test (BSLT) and 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay in the human breast, colon, and liver cancer cell lines were used to evaluate the cytotoxicity activities of the more potent extract. Steroids, flavonoids, tannins, saponins, alkaloids, and cyanogenic glycosides were present in B. pilosa leaf extracts. BE showed higher antioxidant and antimicrobial activities than BA. However, BSLT results showed BA was more toxic than BE based on their lethal concentration 50 (LC50) values after 24- hour exposure. MTT assay also showed that BA was more potent against the colon cancer (HCT116) cell line, followed by breast (MCF-7) and liver (Hep G2) cancer cell lines. The B. pilosa extracts possess potent antioxidant, antimicrobial, and anticancer activities. These biological activities are due to the presence of various natural products present in its leaves. Further investigations are needed to understand the pharmacological properties of B. pilosa fully.

Anahtar Kelimeler

Free radicals HCT 116 human cancer MTT assay traditional medicine

Kaynakça

  • [1] Galvez JZ. The need for national colloquium on medicinal plants research and business opportunities: Proceedings of the seminar on the State of the Art of Medicinal Plant Research and Business Opportunities. Manila, Philippines; 2003.
  • [2] The Tboli Tribe of South Cotabato. Philippines, Mindanao, T’boli woman girls. http://everywheremag.com/articles/1178 (accessed on 15 July 2016).
  • [3] Baay JM, Buhian SD, Diocales AT. BS Thesis. Bioactivity of commonly used medicinal plants by T’bolis of South Cotabato. College of Arts and Sciences, Notre Dame of Dadiangas University, General Santos City, Philippines, 2013.
  • [4] Talavera J, Manalo F, Baybay A, Saludario D, Dizon R, Mauro B, Porquerino A, Novela A, Yakit F, Banares A, Franscisco M, Inocencio R, Rongavilla C, Cruz T. The T’boli: Songs, Stories, and Society. A descriptive study on the T’boli language, lifestyle, marriage, political system, and religion; 2013. https://www.researchgate.net/publication/304347631_The_T'boli_Songs_Stories_and _Society (accessed on 15 July 2024).
  • [5] List of Philippine Medicinal Plants with Chinese Names. http://www.stuartxchange.com/Dadayem.html (accessed on 18 March 2024).
  • [6] Karis PO, Ryding O. Asteraceae Cladistics and Classification, Bremer K. Eds, 559–569, Timber Press, Portland, Oregon, USA 1994.
  • [7] Odhay B, Beekrum S, Akula U, Baijnath H. Preliminary assessment of nutritional value of traditional leafy vegetables in Kwazulu-Natal, South Africa. J Food Compost Anal. 2007; (20): 430-435. https://doi.org/10.1016/j.jfca.2006.04.015
  • [8] Pozharitskaya ON, Shikov AN, Makarova MN, Kosman VM, Faustova NM, Tesakova SV, Makarov VG, Galambosi B. Anti-inflammatory activity of a HPLC-fingerprinted aqueous infusion of aerial part of Bidens tripartita L. Phytomedicine. 2010;17(6):463-468. https://doi.org/10.1016/j.phymed.2009.08.001.
  • [9] Vineela CH, Elizabeth KM. Antimicrobial activity of marine algae of Visakhapatnam City, Andhra Pradesh. Asian J Microbiol Biotechnol Environ Sci. 2005; 7: 209-212.
  • [10] Ekpo MA, Etim PC. Antimicrobial activity of ethanolic and aqueous extracts of Sida acuta on microorganisms from skin infections. J Med Plant Res. 2009; 3(9): 621-624.
  • [11] Tosoc JPS, Frediles VCP, Canda C, Demayo CD. Antiangiogenic, antitoxic and antioxidant properties of methanolic extracts of Caladium bicolor (Aiton) Venten. Hum Vet Med. 2016; 8(1): 10-16.
  • [12] Sofowa A. Medicinal Plants and Traditional Medicine in Africa. 1st ed., New York: John Wiley and Sons Ltd. Hoboken, NJ, USA 1982, pp. 168-171.
  • [13] Arora DS, Kaur J. Antimicrobial activity of spices. Int J Antimicrob Agents. 1999; 12(3): 257-262. https://doi.org/10.1016/S0924-8579(99)00074-6
  • [14] Ahmedulla M, Nayar MP. Red data book of Indian plants: Calcutta: Botanical Survey of India, New Delhi, India 1999.
  • [15] Rios J, Recio M. Medicinal plants, and antimicrobial activity. J Ethnopharmacol. 2005; 100: 80-84. https://doi.org/10.1016/j.jep.2005.04.025
  • [16] Nweze EL, Okafor JI, Njokn O. Antimicrobial activities of methanolic extracts of Trema guinensis (Schumm and Thorn) and Morinda Lucida Benth used in Nigeria. Biology Res. 2004; 2: 39-46. https://doi.org/10.4314/br.v2i1.28540
  • [17] Ashis G. Herbal folk remedies of Bankura and Medinipur districts, West Bengal. Indian Journal of Traditional Knowledge, 2003; 2: 393-396.
  • [18] Adegbite AE, Sanyaolu EB. Cytotoxicity testing of aqueous extracts of bitter leaf (Vernonia amugdalina Del.) using the Allium cepa chromosome aberration assay. Sci Res Essay. 2009; 4(11): 1311-1314. https://doi.org/10.4314/ahs.v17i1.19
  • [19] Dahlberg A, Trygger S. Indigenous medicine and primary health care: The importance of lay knowledge and use of medicinal plants in rural South Africa. Hum Ecol. 2009; 37(1): 79-94. https://dx.doi.org/10.1007/s10745-009-9217-6
  • [20] Uprety Y, Asselin H, Boon E, Yadav S, Shrestha K. Indigenous use and bio-efficacy of medicinal plants in the Rasuwa District, Central Nepal. J Ethnobiol Ethnomed. 2010; 6:3. https://doi.org/10.1186/1746-4269-6-3
  • [21] Romeiras M, Duarte MC, Indjai B, Catarino L. Medicinal plants used to treat neurological disorders in West Africa: A case study with Guinea-Bissau Flora. Am J Plant Sci. 2012; 3(7): 1028-1036. https://doi.org/10.4236/ajps.2012.327122
  • [22] Hossan S, Hanif A, Agarwala B, Sarwar S, Karim M. Traditional use of medicinal plants in Bangladesh to treat urinary tract infections and sexually transmitted diseases. Ethnobot Res App. 2010; 8: 61-74.
  • [23] Yang HL, Chen SC, Chang NW, Chang JM, Lee ML, Tsai PC, Fu HH, Kao WW, Chiang HC, Wang HH, Hseu YC. Protection from oxidative damage using Bidens pilosa extracts in normal human erythrocytes. Food Chem Toxicol. 2006;44(9):1513-1521. https://doi.org/10.1016/j.fct.2006.04.006
  • [24] Devi KP, Suganthy N, Kesika P, Pandian SK. Bioprotective properties of seaweeds: In vitro evaluation of antioxidant activity and antimicrobial activity against food-borne bacteria in relation to polyphenolic content. BMC Complement Altern Med. 2008; 8: 38. https://doi.org/10.1186/1472-6882-8-38
  • [25] Djacbou DS, Anatole PC, Cabral BP, Veronique PB. Comparison of in vitro antioxidant properties of extracts from three plants used for medical purpose in Cameroon: Acalypha racemosa, Garcinia lucida and Hymenocardia lyra. Asian Pac J Trop Biomed. 2014; 4: S625–S632. https://doi.org/10.12980/APJTB.4.201414B168
  • [26] Murillo-Alvarez JI, Encarnacion DR, Franzblau SG. Antimicrobial and cytotoxic activity of some medicinal plants from Baja California Sur (Mexico). Pharm Biol. 2001; 39: 6, 445-449. https://doi.org/10.1076/phbi.39.6.445.5877
  • [27] Deng SX, West BJ, Jensen CJ. A quantitative comparison of phytochemical components in global noni fruits and their commercial products. Food Chem. 2010; 122(1): 267-270. https://doi.org/10.1016/j.foodchem.2010.01.031.
  • [28] Ramakrishna A, Ravishankar GA. Influence of abiotic stress signals on secondary metabolites in plants. Plant Signal Behav. 2011; 6(11): 1720-1731. https://doi.org/10.4161/psb.6.11.17613
  • [29] Pavarini DP, Pavarini SP, Niehues M, Lopes NP. Exogenous influences on plant secondary metabolite levels. Anim Feed Sci Technol. 2012; 176(1–4): 5-16. https://doi.org/10.1016/j.anifeedsci.2012.07.002
  • [30] Chang JS, Chiang LC, Chen CC, Liu LT, Wang KC, Lin CC. Antileukemic activity of Bidens pilosa L. var. minor (Blume) Sherff and Houttuynia cordata Thunb. Am J Chin Med. 2001; 29: 303–312. https://doi.org/10.1142/S0192415X01000320
  • [31] Wu LW, Chiang YM, Chuang HC, Wang SY, Yang GW, Chen YH, Lai LY, Shyur LF. Polyacetylenes function as antiangiogenic agents. Pharm Res. 2004; 21: 2112–2119. https://doi.org/10.1023/b:pham.0000048204.08865.41
  • [32] Aguinaldo AM, Espeso EI, Guevara BQ, Nonato MG. Phytochemistry. In: Guevara, B.Q. (Ed.), A Guidebook to Plant Screening: Phytochemical and Biological. University of Santo Tomas, Manila, Philippines 2005.
  • [33] De Padua LS, Lugod GC, Pancho JV. Handbook of Philippine Medicinal Plants. University of the Philippines, Los Baños 1997.
  • [34] Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem. 1999; 269(2): 337–341. https://doi.org/10.1006/abio.1999.4019
  • [35] Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966; 45(4): 493-496. https://doi.org/10.1093/ajcp/45.4_ts.493
  • [36] Bonev B, Hooper J, Parisot J. Principles of assessing bacterial susceptibility to antibiotics using the agar diffusion method. J Antimicrob Chemother. 2008; 61(6): 1295- 301. https://doi.org/10.1093/jac/dkn090.
  • [37] Meyer BN, Ferrigini NR, Putnam JE, Jacobson LB, Nichols DE, McLaughlin JL. Brine Shrimp: A convenient general bioassay for active plant constituents. Planta Med. 1982; 45: 31-34. https://doi.org/10.1055/s-2007-971236
  • [38] Silva LAB, Azevedo LU, Consolaro A, Barnett F, Xu Y, Battaglino RA, Cañadas PS, de Oliveira KMH, Silva RAB. Novel endodontic sealers induce cell cytotoxicity and apoptosis in a dose-dependent behavior and favorable response in mice subcutaneous tissue. Clin Oral Invest. 2017; 21(9): 2851-2861. https://doi.org/ 10.1007/s00784-017-2087-1
  • [39] Mahmoodinia Maymand M, Soleimanpour-lichaei HR, Ardeshirylajimi A, Soleimani M, Enderami SE, Nojehdehi S, Behjati F, Kabir Salmani M. Improvement of hepatogenic differentiation of iPS cells on an aligned polyethersulfone compared to random nanofibers. Artif Cells Nanomed Biotechnol. 2018; 46(4): 853-860. https://doi.org/10.1080/21691401.2017.1345929
Toplam 39 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Eczacılık ve İlaç Bilimleri (Diğer)
Bölüm Articles
Yazarlar

John Paul Sese Tosoc

Mylene Mondarte Uy

Wtpsk Senarath

Olga Macas Nuñeza

Yayımlanma Tarihi 5 Temmuz 2025
Gönderilme Tarihi 31 Mart 2024
Kabul Tarihi 4 Temmuz 2024
Yayımlandığı Sayı Yıl 2025 Cilt: 29 Sayı: 4

Kaynak Göster

APA Tosoc, J. P. S., Uy, M. M., Senarath, W., Nuñeza, O. M. (2025). Phytochemical screening, antioxidant, antimicrobial, and cytotoxicity evaluation of the leaf extracts of Bidens pilosa by the T’boli tribe in South Cotabato, Philippines. Journal of Research in Pharmacy, 29(4), 1367-1373. https://doi.org/10.12991/jrespharm.1734445
AMA Tosoc JPS, Uy MM, Senarath W, Nuñeza OM. Phytochemical screening, antioxidant, antimicrobial, and cytotoxicity evaluation of the leaf extracts of Bidens pilosa by the T’boli tribe in South Cotabato, Philippines. J. Res. Pharm. Temmuz 2025;29(4):1367-1373. doi:10.12991/jrespharm.1734445
Chicago Tosoc, John Paul Sese, Mylene Mondarte Uy, Wtpsk Senarath, ve Olga Macas Nuñeza. “Phytochemical Screening, Antioxidant, Antimicrobial, and Cytotoxicity Evaluation of the Leaf Extracts of Bidens Pilosa by the T’boli Tribe in South Cotabato, Philippines”. Journal of Research in Pharmacy 29, sy. 4 (Temmuz 2025): 1367-73. https://doi.org/10.12991/jrespharm.1734445.
EndNote Tosoc JPS, Uy MM, Senarath W, Nuñeza OM (01 Temmuz 2025) Phytochemical screening, antioxidant, antimicrobial, and cytotoxicity evaluation of the leaf extracts of Bidens pilosa by the T’boli tribe in South Cotabato, Philippines. Journal of Research in Pharmacy 29 4 1367–1373.
IEEE J. P. S. Tosoc, M. M. Uy, W. Senarath, ve O. M. Nuñeza, “Phytochemical screening, antioxidant, antimicrobial, and cytotoxicity evaluation of the leaf extracts of Bidens pilosa by the T’boli tribe in South Cotabato, Philippines”, J. Res. Pharm., c. 29, sy. 4, ss. 1367–1373, 2025, doi: 10.12991/jrespharm.1734445.
ISNAD Tosoc, John Paul Sese vd. “Phytochemical Screening, Antioxidant, Antimicrobial, and Cytotoxicity Evaluation of the Leaf Extracts of Bidens Pilosa by the T’boli Tribe in South Cotabato, Philippines”. Journal of Research in Pharmacy 29/4 (Temmuz 2025), 1367-1373. https://doi.org/10.12991/jrespharm.1734445.
JAMA Tosoc JPS, Uy MM, Senarath W, Nuñeza OM. Phytochemical screening, antioxidant, antimicrobial, and cytotoxicity evaluation of the leaf extracts of Bidens pilosa by the T’boli tribe in South Cotabato, Philippines. J. Res. Pharm. 2025;29:1367–1373.
MLA Tosoc, John Paul Sese vd. “Phytochemical Screening, Antioxidant, Antimicrobial, and Cytotoxicity Evaluation of the Leaf Extracts of Bidens Pilosa by the T’boli Tribe in South Cotabato, Philippines”. Journal of Research in Pharmacy, c. 29, sy. 4, 2025, ss. 1367-73, doi:10.12991/jrespharm.1734445.
Vancouver Tosoc JPS, Uy MM, Senarath W, Nuñeza OM. Phytochemical screening, antioxidant, antimicrobial, and cytotoxicity evaluation of the leaf extracts of Bidens pilosa by the T’boli tribe in South Cotabato, Philippines. J. Res. Pharm. 2025;29(4):1367-73.