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Anti-proliferative activity and phytochemical study of the methanolic extract from the pods of Gleditsia caspica Desf.

Yıl 2024, Cilt: 28 Sayı: 1, 304 - 312, 28.06.2025

Samira Mirzaeidarband Sara Samadishams Sanaz Hamedeyazdan Zahra Eskandari Atefeh Ebrahimi Sedigheh Bamdad—moghaddam Jaleh Barar Yadollah Omidi Laleh Khodaie , Hossein Nazemiyeh

Öz

Gleditsia caspica Desf. (Caesalpinaceae) is traditionally used to treat diverse diseases. This research paper aims to investigate anti-proliferative properties and phytochemical compounds of methanolic extract from the pods of G. caspica. MTT test was used to measure in vitro anti-proliferative effect of the soxhlet-extracted methanolic extract of G.caspica pods with the concentration ranging from 25-1000 µg/ml against A549 cell line. The extract was applied to SPE, HPLC preparative system, and spectral analysis (1HNMR, 13CNMR, and UV) for fractionation, purification, and identification of phytoconstituents, respectively. The results indicated that the extract prevented proliferation of A549 cell line dependent on time and concentration. After 48 h and 72 h, there was a significant decrease in cell viability initiated from 100 and 500 (µg/ml) concentrations in comparison to untreated control. Phytochemical assessment of the extract resulted in two alkaloids, locustoside A and saikachinoside A, and four flavonoids quercetin-3,7-diglucoside, luteolin-4',7–diglucoside, quercetin-3-glucoside, and quercetin. This experiment was an activity-guided phytochemical study. In prior studies, quercetin and quercetin-3-β-D-glucoside showed in vitro inhibitory effect on proliferation of A549 cell line. However, the cytotoxic properties of the other identified compounds have not been reported. Cytotoxic properties of G.caspica was previously attributed to saponins, while isolated flavonoids and alkaloids could be responsible for the extract’s cytotoxic effects. This paper first explored cytotoxic activity of methanolic extract from the pods of G.caspica on A549 cell line and identified flavonoids and alkaloids of this plant.

Anahtar Kelimeler

Gleditsia caspica Phytochemistry Pod Human lung cancer A549 cell line Apoptosis

Kaynakça

  • [1] Muzaffarian WA. A dictionary of Iranian plant names: Latin, English, Persian = Farhang-i namha-yi giyahan-i Iran : Latini-Inglisi-Farsi, Tehran, Farhang-e Mo`aser. 1996, pp.216.
  • [2] Zhang JP, Tian XH, Yang YX, Liu QX, Wang Q. Gleditsia species: An ethnomedical, phytochemical and pharmacological review. J Ethnopharmacol. 2016 ;178:155-171. https://doi.org/10.1016/j.jep.2015.11.044 .
  • [3] Mohammed RS, Abou Zeid AH, El Hawary SS, Sleem AA, Ashour WE. Flavonoid constituents, cytotoxic and antioxidant activities of Gleditsia triacanthos L. leaves. Saudi J Biol Sci. 2014; 21(6):547-553. https://doi.org/10.1016/j.sjbs.2014.02.002 .
  • [4] Liu Y, Xu Z, Zhang W, Duan J, Jiang J. Characterization of fractional polysaccharides from Gleditsia sinensis and Gleditsia microphylla gums. Molecules. 2016; 21(12): 1745. https://doi.org/10.3390/molecules21121745 .
  • [5] Liu C, Sun H, Wang WT, Zhang JB, Cheng AW, Guo X. A new triterpenoid saponin from Gleditsia sinensis and its antiproliferative activity. Nat Prod Res. 2015; 30(18): 2065-2070. https://doi.org/10.1080/14786419.2015.1110704 .
  • [6] Zhang H, Zhang Y, Wang Y, Zhan R and Chen Y. A new neolignan from the thorns of Gleditsia japonica var. delavayi. Nat Prod Res. 2019; 33(2): 239-243. https://doi.org/10.1080/14786419.2018.1443101 .
  • [7] Sun M, Li Y, Wang T, Sun Y, Xu X. Isolation, fine structure and morphology studies of galactomannan from endosperm of Gleditsia japonica var. delavayi. Carbohydr Polym. 2018; 184: 127-134. https://doi.org/10.1016/j.carbpol.2017.12.003 .
  • [8] Zhou L, Li D, Wang J, Liu Y and Wu J. Antibacterial phenolic compounds from the spines of Gleditsia sinensis Lam. Nat Prod Res. 2007; 21(4): 283-291. https://doi.org/10.1080/14786410701192637 .
  • [9] Chen J, Li Z, Zheng KY, Guo AJ, Zhu KY. Chemical fingerprinting and quantitative analysis of two common Gleditsia sinensis fruits using HPLC-DAD. Acta Pharm. 2013; 63(4): 505-515. https://doi.org/10.2478/acph-2013-0036 .
  • [10] Harauchi Y, Kajimoto T, Ohta E, Kawachi H, Imamura-Jinda. Prenylated purine alkaloids from seeds of Gleditsia japonica. Phytochemistry. 2017;143: 145-150. https://doi.org/10.1016/j.phytochem.2017.08.006 .
  • [11] Kajimoto T, Aoki N, Ohta E, Ohta S. Locustoside A—A new purine alkaloid glucoside from seeds of Gleditsia japonica. Phytochemistry Lett. 2010; 3(4):198-200. https://doi.org/10.1016/j.phytol.2010.07.006 .
  • [12] Kajimoto T, Aoki N, Ohta E, Kawai Y and Ohta S. Saikachinoside A, a novel 3-prenylated isoguanine glucoside from seeds of Gleditsia japonica. Tetrahedron Lett. 2010;51(16): 2099-2101 . https://doi.org/10.1016/j.tetlet.2010.01.090 .
  • [13] Shaheen U, Ragab EA, Abdalla AN and Bader A. Triterpenoidal saponins from the fruits of Gleditsia caspica with proapoptotic properties. Phytochemistry. 2018; 145:168-178. https://doi.org/10.1016/j.phytochem.2017.11.007 .
  • [14] Melek FR, Aly FA, Kassem IA, Abo-Zeid MA, Farghaly AA. Three further triterpenoid saponins from Gleditsia caspica fruits and protective effect of the total saponin fraction on cyclophosphamide-induced genotoxicity in mice. Z Naturforsch C J Biosci. 2015;70(1-2): 31-37. https://doi.org/10.1515/znc-2014-4132 .
  • [15] Melek FR, Kassem IA, Miyase T and Fayad W. Caspicaosides E-K, triterpenoid saponins and cytotoxic acylated saponins from fruits of Gleditsia caspica Desf. Phytochemistry. 2014; 100:110-119. https://doi.org/10.1016/j.phytochem.2014.01.019 .
  • [16] Yu J, Xian Y, Li G, Wang D, Zhou H. One new flavanocoumarin from the thorns of Gleditsia sinensis. Nat Prod Res. 2017; 31(3): 275-280. https://doi.org/10.1080/14786419.2016.1233406 .

Yıl 2024, Cilt: 28 Sayı: 1, 304 - 312, 28.06.2025

Samira Mirzaeidarband Sara Samadishams Sanaz Hamedeyazdan Zahra Eskandari Atefeh Ebrahimi Sedigheh Bamdad—moghaddam Jaleh Barar Yadollah Omidi Laleh Khodaie , Hossein Nazemiyeh

Öz

Kaynakça

  • [1] Muzaffarian WA. A dictionary of Iranian plant names: Latin, English, Persian = Farhang-i namha-yi giyahan-i Iran : Latini-Inglisi-Farsi, Tehran, Farhang-e Mo`aser. 1996, pp.216.
  • [2] Zhang JP, Tian XH, Yang YX, Liu QX, Wang Q. Gleditsia species: An ethnomedical, phytochemical and pharmacological review. J Ethnopharmacol. 2016 ;178:155-171. https://doi.org/10.1016/j.jep.2015.11.044 .
  • [3] Mohammed RS, Abou Zeid AH, El Hawary SS, Sleem AA, Ashour WE. Flavonoid constituents, cytotoxic and antioxidant activities of Gleditsia triacanthos L. leaves. Saudi J Biol Sci. 2014; 21(6):547-553. https://doi.org/10.1016/j.sjbs.2014.02.002 .
  • [4] Liu Y, Xu Z, Zhang W, Duan J, Jiang J. Characterization of fractional polysaccharides from Gleditsia sinensis and Gleditsia microphylla gums. Molecules. 2016; 21(12): 1745. https://doi.org/10.3390/molecules21121745 .
  • [5] Liu C, Sun H, Wang WT, Zhang JB, Cheng AW, Guo X. A new triterpenoid saponin from Gleditsia sinensis and its antiproliferative activity. Nat Prod Res. 2015; 30(18): 2065-2070. https://doi.org/10.1080/14786419.2015.1110704 .
  • [6] Zhang H, Zhang Y, Wang Y, Zhan R and Chen Y. A new neolignan from the thorns of Gleditsia japonica var. delavayi. Nat Prod Res. 2019; 33(2): 239-243. https://doi.org/10.1080/14786419.2018.1443101 .
  • [7] Sun M, Li Y, Wang T, Sun Y, Xu X. Isolation, fine structure and morphology studies of galactomannan from endosperm of Gleditsia japonica var. delavayi. Carbohydr Polym. 2018; 184: 127-134. https://doi.org/10.1016/j.carbpol.2017.12.003 .
  • [8] Zhou L, Li D, Wang J, Liu Y and Wu J. Antibacterial phenolic compounds from the spines of Gleditsia sinensis Lam. Nat Prod Res. 2007; 21(4): 283-291. https://doi.org/10.1080/14786410701192637 .
  • [9] Chen J, Li Z, Zheng KY, Guo AJ, Zhu KY. Chemical fingerprinting and quantitative analysis of two common Gleditsia sinensis fruits using HPLC-DAD. Acta Pharm. 2013; 63(4): 505-515. https://doi.org/10.2478/acph-2013-0036 .
  • [10] Harauchi Y, Kajimoto T, Ohta E, Kawachi H, Imamura-Jinda. Prenylated purine alkaloids from seeds of Gleditsia japonica. Phytochemistry. 2017;143: 145-150. https://doi.org/10.1016/j.phytochem.2017.08.006 .
  • [11] Kajimoto T, Aoki N, Ohta E, Ohta S. Locustoside A—A new purine alkaloid glucoside from seeds of Gleditsia japonica. Phytochemistry Lett. 2010; 3(4):198-200. https://doi.org/10.1016/j.phytol.2010.07.006 .
  • [12] Kajimoto T, Aoki N, Ohta E, Kawai Y and Ohta S. Saikachinoside A, a novel 3-prenylated isoguanine glucoside from seeds of Gleditsia japonica. Tetrahedron Lett. 2010;51(16): 2099-2101 . https://doi.org/10.1016/j.tetlet.2010.01.090 .
  • [13] Shaheen U, Ragab EA, Abdalla AN and Bader A. Triterpenoidal saponins from the fruits of Gleditsia caspica with proapoptotic properties. Phytochemistry. 2018; 145:168-178. https://doi.org/10.1016/j.phytochem.2017.11.007 .
  • [14] Melek FR, Aly FA, Kassem IA, Abo-Zeid MA, Farghaly AA. Three further triterpenoid saponins from Gleditsia caspica fruits and protective effect of the total saponin fraction on cyclophosphamide-induced genotoxicity in mice. Z Naturforsch C J Biosci. 2015;70(1-2): 31-37. https://doi.org/10.1515/znc-2014-4132 .
  • [15] Melek FR, Kassem IA, Miyase T and Fayad W. Caspicaosides E-K, triterpenoid saponins and cytotoxic acylated saponins from fruits of Gleditsia caspica Desf. Phytochemistry. 2014; 100:110-119. https://doi.org/10.1016/j.phytochem.2014.01.019 .
  • [16] Yu J, Xian Y, Li G, Wang D, Zhou H. One new flavanocoumarin from the thorns of Gleditsia sinensis. Nat Prod Res. 2017; 31(3): 275-280. https://doi.org/10.1080/14786419.2016.1233406 .
Toplam 16 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Eczacılık ve İlaç Bilimleri (Diğer)
Bölüm Articles
Yazarlar

Samira Mirzaeidarband 0009-0007-2572-1962

Sara Samadishams 0009-0009-1713-4009

Sanaz Hamedeyazdan 0000-0002-8950-9879

Zahra Eskandari 0009-0001-8455-2237

Atefeh Ebrahimi 0000-0003-0172-1283

Sedigheh Bamdad—moghaddam 0000-0002-7320-8908

Jaleh Barar 0000-0002-0105-919X

Yadollah Omidi 0000-0003-0067-2475

Laleh Khodaie 0000-0001-7416-0822

Hossein Nazemiyeh 0000-0003-0165-3209

Yayımlanma Tarihi 28 Haziran 2025
Yayımlandığı Sayı Yıl 2024 Cilt: 28 Sayı: 1

Kaynak Göster

APA Mirzaeidarband, S., Samadishams, S., Hamedeyazdan, S., Eskandari, Z., vd. (2025). Anti-proliferative activity and phytochemical study of the methanolic extract from the pods of Gleditsia caspica Desf. Journal of Research in Pharmacy, 28(1), 304-312.
AMA Mirzaeidarband S, Samadishams S, Hamedeyazdan S, Eskandari Z, Ebrahimi A, Bamdad—moghaddam S, Barar J, Omidi Y, Khodaie L, Nazemiyeh H. Anti-proliferative activity and phytochemical study of the methanolic extract from the pods of Gleditsia caspica Desf. J. Res. Pharm. Haziran 2025;28(1):304-312.
Chicago Mirzaeidarband, Samira, Sara Samadishams, Sanaz Hamedeyazdan, Zahra Eskandari, Atefeh Ebrahimi, Sedigheh Bamdad—moghaddam, Jaleh Barar, Yadollah Omidi, Laleh Khodaie, ve Hossein Nazemiyeh. “Anti-Proliferative Activity and Phytochemical Study of the Methanolic Extract from the Pods of Gleditsia Caspica Desf”. Journal of Research in Pharmacy 28, sy. 1 (Haziran 2025): 304-12.
EndNote Mirzaeidarband S, Samadishams S, Hamedeyazdan S, Eskandari Z, Ebrahimi A, Bamdad—moghaddam S, Barar J, Omidi Y, Khodaie L, Nazemiyeh H (01 Haziran 2025) Anti-proliferative activity and phytochemical study of the methanolic extract from the pods of Gleditsia caspica Desf. Journal of Research in Pharmacy 28 1 304–312.
IEEE S. Mirzaeidarband, “Anti-proliferative activity and phytochemical study of the methanolic extract from the pods of Gleditsia caspica Desf”., J. Res. Pharm., c. 28, sy. 1, ss. 304–312, 2025.
ISNAD Mirzaeidarband, Samira vd. “Anti-Proliferative Activity and Phytochemical Study of the Methanolic Extract from the Pods of Gleditsia Caspica Desf”. Journal of Research in Pharmacy 28/1 (Haziran 2025), 304-312.
JAMA Mirzaeidarband S, Samadishams S, Hamedeyazdan S, Eskandari Z, Ebrahimi A, Bamdad—moghaddam S, Barar J, Omidi Y, Khodaie L, Nazemiyeh H. Anti-proliferative activity and phytochemical study of the methanolic extract from the pods of Gleditsia caspica Desf. J. Res. Pharm. 2025;28:304–312.
MLA Mirzaeidarband, Samira vd. “Anti-Proliferative Activity and Phytochemical Study of the Methanolic Extract from the Pods of Gleditsia Caspica Desf”. Journal of Research in Pharmacy, c. 28, sy. 1, 2025, ss. 304-12.
Vancouver Mirzaeidarband S, Samadishams S, Hamedeyazdan S, Eskandari Z, Ebrahimi A, Bamdad—moghaddam S, Barar J, Omidi Y, Khodaie L, Nazemiyeh H. Anti-proliferative activity and phytochemical study of the methanolic extract from the pods of Gleditsia caspica Desf. J. Res. Pharm. 2025;28(1):304-12.