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Yıl 2020, Cilt: 24 Sayı: 2, 233 - 239, 27.06.2025

Gökalp İşcan , Betül Demirci , Yavuz Bülent Köse

https://doi.org/10.35333/jrp.2020.140

Öz

Kaynakça

  • [1] Ietswaart JH. A Taxonomic Revision of the Genus Origanum (Labiatae). Leiden Botanical Series 4. Leiden University Press, The Hague, The Netherlands 1980
  • [2] Kokkini S. Taxonomy, diversity and distribution of Origanum species, In: Padulosi S. (Ed.). Oregano. 14th Proceedings of the IPGRI International Workshop on Oregano, 8-12 May 1996, CIHEAM, Valenzano (Bari), 1996. pp.2-12.
  • [3] Sadıkoğlu N, Özhatay N. Morphological characteristics of exported taxa as Oregano from Turkey I: Origanum. J Faculty Pharm Istanbul Univ. 2015; 45(2): 87-126.
  • [4] Başer KHC. The Turkish Origanum species. In: Kintzios E. (Ed.) Oregano: The genera Origanum and Lippia. Taylor & Francis; London, 2002, pp.109-126.
  • [5] Başer KHC, Özek T, Tümen G, Sezik, E. Composition of the essential oils of Turkish Origanum species with commercial importance. J Essent Oil Res. 1993; 5: 619-623. [CrossRef]
  • [6] Baricevic D, Bartol T. The biological/pharmacological activity of the Origanum genus. In: Kintzios E. (Ed.) Oregano: the genera Origanum and Lippia. Taylor and Francis, London, 2002, pp.177-214.
  • [7] Şahin F, Güllüce M., Daferera D, Sökmen A, Sökmen M, Polissiou M, Agar G, Özer H. Biological activities of the essential oils and methanol extract of Origanum vulgare ssp. vulgare in the Eastern Anatolia region of Turkey. Food Control, 2004; 15(7): 549-557. [CrossRef]
  • [8] Chishti S, Kaloo ZA, Sultan P. Medicinal importance of genus Origanum: A review. J Pharmacognosy Phytother. 2013; 5(10): 170-177.
  • [9] Ličina BZ, Stefanović OD, Vasić SM, Radojević ID, Dekić MS, Čomić LR. Biological activities of the extracts from wild growing Origanum vulgare L. Food Control. 2013; 33(2): 498-504. [CrossRef]
  • [10] Han X, Parker TL. Anti-inflammatory, tissue remodeling, immunomodulatory, and anticancer activities of oregano (Origanum vulgare) essential oil in a human skin disease model. Biochimie Open. 2017; 4: 73-77. [CrossRef]
  • [11] Elshafie HS, Armentano MF, Carmosino M, Bufo SA, De Feo V, Camele I. Cytotoxic activity of Origanum vulgare L. on hepatocellular carcinoma cell line HepG2 and evaluation of its biological activity. Molecules. 2017; 22(9): 1435. [CrossRef]
  • [12] Adam K, Sivropoulou A, Kokkini S, Lanaras T, Arsenakis M. Antifungal activities of Origanum vulgare subsp. hirtum, Mentha spicata, Lavandula angustifolia, and Salvia fruticosa essential oils against human pathogenic fungi. J Agric Food Chem. 1998; 46(5): 1739-1745. [CrossRef]
  • [13] Bakkali F, Averbeck S, Averbeck D, Idaomar M. Biological effects of essential oils–a review. Food Chem Toxicol. 2008; 46(2): 446-475. [CrossRef]
  • [14] Soković M, Glamočlija J, Marin PD, Brkić D, van Griensven LJ. Antibacterial effects of the essential oils of commonly consumed medicinal herbs using an in vitro model. Molecules. 2010; 15(11): 7532-7546. [CrossRef]
  • [15] Leyva-López N, Gutiérrez-Grijalva EP, Vazquez-Olivo G, Heredia JB. Essential oils of oregano: Biological activity beyond their antimicrobial properties. Molecules. 2017; 22(6): 989. [CrossRef]
  • [16]Lambert RJ, Skandamis PN, Coote PJ, Nychas GJ. A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol, J Appl Microbiol. 2001; 91: 453 462. CrossRef
  • [17]Nostro A, Blanco AR, Cannatelli MA, Enea V, Flamini G, Morelli I, Sudano RA, Alonzo V. Susceptibility of methicillin resistant Staphylococci to oregano essential oil, carvacrol and thymol. FEMS Micro Lett. 2004; 230: 191 195. CrossRef
  • [18]Nostro A, Roccaro SA, Bisignano G, Marino A, Cannatelli MA, Pizzimenti FC, Cioni PL, Procopio F, Blanco AR. Effects of oregano, carvacrol and thymol on Staphylococcus aureus and Staphylococcus epidermidis biofilms. J Med Microbiol. 2007; (56)4: 519 523. CrossRef
  • [19]Kotan R, Kordali S, Çakır A. Screening of antibacterial activities of twenty one oxygenated monoterpe nes. Z Naturforsch C. 2007; 62(7 8): 507 513. CrossRef
  • [20]Xu J, Zhou F, Ji BP, Pei RS, Xu N. The antibacterial mechanism of carvacrol and thymol against Escherichia coli. Letters App Microbiol. 2008 ; 47, 174 179. CrossRef
  • [21] Carson CF, Hammer KA. Chemistry and Bioactivity of Essential Oils, In: Thormar H. (Ed.) Lipids and Essential Oils as Antimicrobial Agents. John Wiley & Sons, Ltd., New York, 2011. pp.203-238.
  • [22]De Martino L, De Feo V, Fratianni F, Nazzaro F. Chemistry, antioxidant, antibacterial and antifungal activities of volatile oils and their components. Nat Prod Commun. 2009; 4 : 1741 1746. CrossRef
  • [23]Du E, Gan L, Li Z, Wang W, Liu D, Guo Y. In vitro antibacterial activity of thymol and carvacrol and their effects on broiler chickens challenged with Clostridium perfringens. J Anim Sci Biotechnol. 2015; 6: 58. CrossRef
  • [24] Khan ST, Khan M, Ahmad J, Wahab R, Abd-Elkader OH., Musarrat J, Alkhathlan HZ, Al-Kedhairy AA. Thymol and carvacrol induce autolysis, stress, growth inhibition and reduce the biofilm formation by Streptococcus mutans. AMB Express. 2017; 7(1): 49. [CrossRef]
  • [25]Edris AE. Pharmaceutical and therapeutic potentials of essential oils and their ind ividual volatile constituents: A review. Phytother Res. 2007; 21: 308 323. CrossRef
  • [26] Ietswaart JH. Origanum L. In: Davis PH. (Ed.). Vol. 7 Flora of Turkey and the East Aegean Islands: Edinburgh Univ. Press, Edinburgh, 1982, pp.299.
  • [27] Başer KHC, Duman H. Composition of the Essential Oils of Origanum boissieri letswaart and O. bargyli Mouterde. J Essent Oil Res. 1998; 10: 71-72. [CrossRef]
  • [28] McLafferty FW, Stauffer DB. The Wiley/NBS Registry of Mass Spectral Data, J Wiley and Sons, New York, pp.1989.
  • [29] Koenig WA, Joulain D, Hochmuth DH. Terpenoids and Related Constituents of Essential Oils. MassFinder 3, Hamburg, Germany, 2004.
  • [30] Joulain D, Koenig WA. The Atlas of Spectra Data of Sesquiterpene Hydrocarbons, EB-Verlag, Hamburg, 1998, pp.10-658.
  • [31] ESO 2000. (The Complete Database of Essential Oils) program, https://www.leffingwell.com/virtual_xp_mode.htm (accessed on 12 June 2017).
  • [32] National Committee for Clinical Laboratory Standards. Reference method for broth dilution antifungal susceptibility testing of yeasts. Approved Standard-M27A2. CLSI, Wayne PA, USA. 2002.
  • [33] National Committee for Clinical Laboratory Standards. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically. Approved Standard-M7A7. Wayne PA, USA. 2006.
  • [34] Rahalison L, Hamburger M, Hostettmann K, Monod M, Frenk EA, Bioautographic agar overlay method for the detection of antifungal compounds from higher plants. Phytochem Anal. 1991; 2: 199-203. [CrossRef]
  • [35] Hostettmann K. Strategy for the biological evaluation of plant extracts. Pure App Chem. 1999; 70: 1109-1113.
  • [36] İşcan G. Antibacterial and anticandidal activities of common essential oil constituents. Rec Nat Prod. 2017; 11(4): 374-388. [CrossRef]
  • [37] Nazzaro F, Fratianni F, De Martino L, Coppola R, De Feo V. Effect of essential oils on pathogenic bacteria. Pharmaceuticals (Basel). 2013; 6: 1451-1474. [CrossRef]
  • [38] Souza NAB, Lima EDO, Guedes DN, Pereira, FDO, Souza ELD, Sousa FBD. Efficacy of Origanum essential oils for inhibition of potentially pathogenic fungi. Braz J Pharm Sci. 2010; 46(3): 499-508. [CrossRef]

Antimicrobial essential oil of Origanum boissieri Ietswaart

Yıl 2020, Cilt: 24 Sayı: 2, 233 - 239, 27.06.2025

Gökalp İşcan , Betül Demirci , Yavuz Bülent Köse

https://doi.org/10.35333/jrp.2020.140

Öz

Apart from being a popular spice, Origanum species is very important taxa as an herbal medicine for their several pharmacological activities. Essential oils of Origanum species bearing phenolic monoterpenoids such as carvacrol and thymol demonstrated strong antimicrobial properties. In the present study hydrodistilled essential oil of endemic species Origanum boissieri Ietswaart were analysed by GC and GC/MS techniques simultaneously. Thirty compounds representing 95.1 % of the total oil were identified. Carvacrol (39%) and p-cymene (32%) were detected as the main components of the oil. Total methanol extract and essential oil of the flowering aerial parts of the plant with the carvacrol and p-cymene were screened for its antibacterial and anticandidal properties by using microdilution broth assay. Bacillus cereus, Bacillus subtilis, Staphylococcus aureus, Staphlylococcus epidermidis and Listeria monocytogenes were strongly inhibited by the oil between the concentrations of 15,6 - 62.5 μg/mL. O. boissieri extract and the oil showed moderate effects against all tested Candida strains having MIC values of 125-1000 μg/mL.

Anahtar Kelimeler

Origanum boissieri essential oil MIC antimicrobial GC/MS

Kaynakça

  • [1] Ietswaart JH. A Taxonomic Revision of the Genus Origanum (Labiatae). Leiden Botanical Series 4. Leiden University Press, The Hague, The Netherlands 1980
  • [2] Kokkini S. Taxonomy, diversity and distribution of Origanum species, In: Padulosi S. (Ed.). Oregano. 14th Proceedings of the IPGRI International Workshop on Oregano, 8-12 May 1996, CIHEAM, Valenzano (Bari), 1996. pp.2-12.
  • [3] Sadıkoğlu N, Özhatay N. Morphological characteristics of exported taxa as Oregano from Turkey I: Origanum. J Faculty Pharm Istanbul Univ. 2015; 45(2): 87-126.
  • [4] Başer KHC. The Turkish Origanum species. In: Kintzios E. (Ed.) Oregano: The genera Origanum and Lippia. Taylor & Francis; London, 2002, pp.109-126.
  • [5] Başer KHC, Özek T, Tümen G, Sezik, E. Composition of the essential oils of Turkish Origanum species with commercial importance. J Essent Oil Res. 1993; 5: 619-623. [CrossRef]
  • [6] Baricevic D, Bartol T. The biological/pharmacological activity of the Origanum genus. In: Kintzios E. (Ed.) Oregano: the genera Origanum and Lippia. Taylor and Francis, London, 2002, pp.177-214.
  • [7] Şahin F, Güllüce M., Daferera D, Sökmen A, Sökmen M, Polissiou M, Agar G, Özer H. Biological activities of the essential oils and methanol extract of Origanum vulgare ssp. vulgare in the Eastern Anatolia region of Turkey. Food Control, 2004; 15(7): 549-557. [CrossRef]
  • [8] Chishti S, Kaloo ZA, Sultan P. Medicinal importance of genus Origanum: A review. J Pharmacognosy Phytother. 2013; 5(10): 170-177.
  • [9] Ličina BZ, Stefanović OD, Vasić SM, Radojević ID, Dekić MS, Čomić LR. Biological activities of the extracts from wild growing Origanum vulgare L. Food Control. 2013; 33(2): 498-504. [CrossRef]
  • [10] Han X, Parker TL. Anti-inflammatory, tissue remodeling, immunomodulatory, and anticancer activities of oregano (Origanum vulgare) essential oil in a human skin disease model. Biochimie Open. 2017; 4: 73-77. [CrossRef]
  • [11] Elshafie HS, Armentano MF, Carmosino M, Bufo SA, De Feo V, Camele I. Cytotoxic activity of Origanum vulgare L. on hepatocellular carcinoma cell line HepG2 and evaluation of its biological activity. Molecules. 2017; 22(9): 1435. [CrossRef]
  • [12] Adam K, Sivropoulou A, Kokkini S, Lanaras T, Arsenakis M. Antifungal activities of Origanum vulgare subsp. hirtum, Mentha spicata, Lavandula angustifolia, and Salvia fruticosa essential oils against human pathogenic fungi. J Agric Food Chem. 1998; 46(5): 1739-1745. [CrossRef]
  • [13] Bakkali F, Averbeck S, Averbeck D, Idaomar M. Biological effects of essential oils–a review. Food Chem Toxicol. 2008; 46(2): 446-475. [CrossRef]
  • [14] Soković M, Glamočlija J, Marin PD, Brkić D, van Griensven LJ. Antibacterial effects of the essential oils of commonly consumed medicinal herbs using an in vitro model. Molecules. 2010; 15(11): 7532-7546. [CrossRef]
  • [15] Leyva-López N, Gutiérrez-Grijalva EP, Vazquez-Olivo G, Heredia JB. Essential oils of oregano: Biological activity beyond their antimicrobial properties. Molecules. 2017; 22(6): 989. [CrossRef]
  • [16]Lambert RJ, Skandamis PN, Coote PJ, Nychas GJ. A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol, J Appl Microbiol. 2001; 91: 453 462. CrossRef
  • [17]Nostro A, Blanco AR, Cannatelli MA, Enea V, Flamini G, Morelli I, Sudano RA, Alonzo V. Susceptibility of methicillin resistant Staphylococci to oregano essential oil, carvacrol and thymol. FEMS Micro Lett. 2004; 230: 191 195. CrossRef
  • [18]Nostro A, Roccaro SA, Bisignano G, Marino A, Cannatelli MA, Pizzimenti FC, Cioni PL, Procopio F, Blanco AR. Effects of oregano, carvacrol and thymol on Staphylococcus aureus and Staphylococcus epidermidis biofilms. J Med Microbiol. 2007; (56)4: 519 523. CrossRef
  • [19]Kotan R, Kordali S, Çakır A. Screening of antibacterial activities of twenty one oxygenated monoterpe nes. Z Naturforsch C. 2007; 62(7 8): 507 513. CrossRef
  • [20]Xu J, Zhou F, Ji BP, Pei RS, Xu N. The antibacterial mechanism of carvacrol and thymol against Escherichia coli. Letters App Microbiol. 2008 ; 47, 174 179. CrossRef
  • [21] Carson CF, Hammer KA. Chemistry and Bioactivity of Essential Oils, In: Thormar H. (Ed.) Lipids and Essential Oils as Antimicrobial Agents. John Wiley & Sons, Ltd., New York, 2011. pp.203-238.
  • [22]De Martino L, De Feo V, Fratianni F, Nazzaro F. Chemistry, antioxidant, antibacterial and antifungal activities of volatile oils and their components. Nat Prod Commun. 2009; 4 : 1741 1746. CrossRef
  • [23]Du E, Gan L, Li Z, Wang W, Liu D, Guo Y. In vitro antibacterial activity of thymol and carvacrol and their effects on broiler chickens challenged with Clostridium perfringens. J Anim Sci Biotechnol. 2015; 6: 58. CrossRef
  • [24] Khan ST, Khan M, Ahmad J, Wahab R, Abd-Elkader OH., Musarrat J, Alkhathlan HZ, Al-Kedhairy AA. Thymol and carvacrol induce autolysis, stress, growth inhibition and reduce the biofilm formation by Streptococcus mutans. AMB Express. 2017; 7(1): 49. [CrossRef]
  • [25]Edris AE. Pharmaceutical and therapeutic potentials of essential oils and their ind ividual volatile constituents: A review. Phytother Res. 2007; 21: 308 323. CrossRef
  • [26] Ietswaart JH. Origanum L. In: Davis PH. (Ed.). Vol. 7 Flora of Turkey and the East Aegean Islands: Edinburgh Univ. Press, Edinburgh, 1982, pp.299.
  • [27] Başer KHC, Duman H. Composition of the Essential Oils of Origanum boissieri letswaart and O. bargyli Mouterde. J Essent Oil Res. 1998; 10: 71-72. [CrossRef]
  • [28] McLafferty FW, Stauffer DB. The Wiley/NBS Registry of Mass Spectral Data, J Wiley and Sons, New York, pp.1989.
  • [29] Koenig WA, Joulain D, Hochmuth DH. Terpenoids and Related Constituents of Essential Oils. MassFinder 3, Hamburg, Germany, 2004.
  • [30] Joulain D, Koenig WA. The Atlas of Spectra Data of Sesquiterpene Hydrocarbons, EB-Verlag, Hamburg, 1998, pp.10-658.
  • [31] ESO 2000. (The Complete Database of Essential Oils) program, https://www.leffingwell.com/virtual_xp_mode.htm (accessed on 12 June 2017).
  • [32] National Committee for Clinical Laboratory Standards. Reference method for broth dilution antifungal susceptibility testing of yeasts. Approved Standard-M27A2. CLSI, Wayne PA, USA. 2002.
  • [33] National Committee for Clinical Laboratory Standards. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically. Approved Standard-M7A7. Wayne PA, USA. 2006.
  • [34] Rahalison L, Hamburger M, Hostettmann K, Monod M, Frenk EA, Bioautographic agar overlay method for the detection of antifungal compounds from higher plants. Phytochem Anal. 1991; 2: 199-203. [CrossRef]
  • [35] Hostettmann K. Strategy for the biological evaluation of plant extracts. Pure App Chem. 1999; 70: 1109-1113.
  • [36] İşcan G. Antibacterial and anticandidal activities of common essential oil constituents. Rec Nat Prod. 2017; 11(4): 374-388. [CrossRef]
  • [37] Nazzaro F, Fratianni F, De Martino L, Coppola R, De Feo V. Effect of essential oils on pathogenic bacteria. Pharmaceuticals (Basel). 2013; 6: 1451-1474. [CrossRef]
  • [38] Souza NAB, Lima EDO, Guedes DN, Pereira, FDO, Souza ELD, Sousa FBD. Efficacy of Origanum essential oils for inhibition of potentially pathogenic fungi. Braz J Pharm Sci. 2010; 46(3): 499-508. [CrossRef]
Toplam 38 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Farmakognozi
Bölüm Articles
Yazarlar

Gökalp İşcan

Betül Demirci

Yavuz Bülent Köse

Yayımlanma Tarihi 27 Haziran 2025
Yayımlandığı Sayı Yıl 2020 Cilt: 24 Sayı: 2

Kaynak Göster

APA İşcan, G., Demirci, B., & Köse, Y. B. (2025). Antimicrobial essential oil of Origanum boissieri Ietswaart. Journal of Research in Pharmacy, 24(2), 233-239. https://doi.org/10.35333/jrp.2020.140
AMA İşcan G, Demirci B, Köse YB. Antimicrobial essential oil of Origanum boissieri Ietswaart. J. Res. Pharm. Haziran 2025;24(2):233-239. doi:10.35333/jrp.2020.140
Chicago İşcan, Gökalp, Betül Demirci, ve Yavuz Bülent Köse. “Antimicrobial Essential Oil of Origanum Boissieri Ietswaart”. Journal of Research in Pharmacy 24, sy. 2 (Haziran 2025): 233-39. https://doi.org/10.35333/jrp.2020.140.
EndNote İşcan G, Demirci B, Köse YB (01 Haziran 2025) Antimicrobial essential oil of Origanum boissieri Ietswaart. Journal of Research in Pharmacy 24 2 233–239.
IEEE G. İşcan, B. Demirci, ve Y. B. Köse, “Antimicrobial essential oil of Origanum boissieri Ietswaart”, J. Res. Pharm., c. 24, sy. 2, ss. 233–239, 2025, doi: 10.35333/jrp.2020.140.
ISNAD İşcan, Gökalp vd. “Antimicrobial Essential Oil of Origanum Boissieri Ietswaart”. Journal of Research in Pharmacy 24/2 (Haziran 2025), 233-239. https://doi.org/10.35333/jrp.2020.140.
JAMA İşcan G, Demirci B, Köse YB. Antimicrobial essential oil of Origanum boissieri Ietswaart. J. Res. Pharm. 2025;24:233–239.
MLA İşcan, Gökalp vd. “Antimicrobial Essential Oil of Origanum Boissieri Ietswaart”. Journal of Research in Pharmacy, c. 24, sy. 2, 2025, ss. 233-9, doi:10.35333/jrp.2020.140.
Vancouver İşcan G, Demirci B, Köse YB. Antimicrobial essential oil of Origanum boissieri Ietswaart. J. Res. Pharm. 2025;24(2):233-9.