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Comparative phytochemical evaluation of natural and micropropagated plants of Bacopa monnieri (L.)

Yıl 2018, Cilt: 22 Sayı: 1, 66 - 73, 27.06.2025

Firoj Tamboli , Vinod D. Rangari Suresh G. Killedar Sagar U. Jadhav Trupti S. Ghatage Vishwajeet P. Kore

Öz

Bacopa monnieri Linn. an important medicinal plant of Scrophulariaceae family and is distributed in the wet and marshy lands throughout India, Nepal, Sri Lanka, China, Taiwan, and Hawaii. It is endangered species so it is necessary to preserve germplasm. The objective of this research work is the comparative phytochemical evaluation of natural and micro-propagated plants of Bacopa monnieri (BM). It shows the presence of various phytoconstituents namely, alkaloids, saponins, phenolics, and flavonoids. These phytoconstituents are responsible for anti-oxidant and various other activity of the plant. The protocols for tissue culture has been developed for regeneration of the BM plant from small part (nodal segment). The regenerated plant having the similar potential in terms of phytoconstituents. The protocols of Murashige and Skoog medium can be used for the clonal regeneration of plant on the large scale through which it can be protected and preserved for the long duration in small space. From the comparative phytochemical investigation by HPTLC, the phytoconstituents which occur in the micro propagated plant are similar to that of the natural plant of B. monnieri.

Anahtar Kelimeler

bacopa monnieri Linn flavonoids HPTLC Micropropagation Brahmi

Kaynakça

  • [1] Dey KK. An Introduction to Plant Tissue Culture, New Central Book Agency (P) Ltd., Calcutta, India, 1997, pp. 35-44.
  • [2] Narayanswamy S. Plant Cell and Tissue Culture, Tata McGraw-Hill Education, New Delhi, India, 1994, pp. 22-51.
  • [3] Chatterji N, Rastogi RP, Dhar ML. Chemical examination of Bacopa monnieria Wettst: Part 1 – Isolation of chemical constituents. Indian J Chem. 1963; 1: 212-215.
  • [4] Asolkar LV, Kakkar KK, Chakre OS. Second Supplement to Glossary of Indian Medicinal Plants with Active Principles (Part I), CSIR, New Delhi, India, 1992, pp. 112-113.
  • [5] Nadkarni KM. The Indian Materia Medica I, Popular Prakashan, Mumbai, India, 1982, pp. 624-625.
  • [6] Sala AV. Indian Medicinal Plants, Orient Longman, Kottakkal, India, 1994, pp. 235-236.
  • [7] Mishra SK, Tiwari KN, Shivna PL, Mishra AK. Micropropagation and comparative phytochemical, antioxidant study of Bacopa monnieri (L.) Pennell. Res J Pharm Biol Chem Sci. 2015; 6(6): 902-912.
  • [8] Ambasta SP. Wealth of India Raw Materials, Vol. 2:B. Council of Scientific and Industrial Research, New Delhi, India, 1988, pp. 23-24.
  • [9] Jain SK, DeFilipps RA. Medicinal Plants of India, Vol. 2. Reference Publication Inc., Michigan, USA, 1991, pp. 556-557.
  • [10] Soundararajan T, Karrunakaran CM. Micropropagation of Bacopa monnieri through protoplast. Asian J Biotechnol. 2011; 3(2): 135-152.
  • [11] Heble MR. Production of secondary metabolites through tissue culture and its prospects for commercial use. In: Islam AS, editor. Plant Tissue Culture, Oxford and IBH Publishing Company Pvt. Ltd., New Delhi, 1997, pp. 161-168.
  • [12] George J, Ravishankar GA. Harnessing high value metabolites from plant cells. Ind J Pharm Educ. 1996; 30: 120-130.
  • [13] Fowler HW. Extraction. In: Carter SJ, editor. Cooper and Gunn’s Tutorial Pharmacy, 6th ed. CBS Publishers and Distributors, New Delhi, 1986, pp. 251-261.
  • [14] Yelne MB, Borkar GB, Sharma PC. In vitro propagation of Brahmi – Bacopa monnieri (L.) Pennell. Bull Medico-Ethno-Bot Res. 1997; 18(3-4): 45-50.
  • [15] Stahl E. Thin Layer Chromatography, 2nd ed., Springer-Verlag, Berlin Heidelberg, Germany, 1969, pp. 904-905.
  • [16] Srivastava VK, Srivastava KK. Introduction to Chromatography, S. Chand and Company Ltd., Delhi, India, 1991, pp. 8-32.
  • [17] Sethi PD. High-performance Thin Layer Chromatography, CBS Publisher and Distributors, New Delhi, India, 1996, pp. 4-28.
  • [18] Narayana DBA, Agarwal S, Luthra SK, Srinivas NS. A HPTLC method for the quantitative analysis of vasicine in Adhatoda vasica. Indian Drugs. 1995; 32: 583-586.
  • [19] Chauhan SK, Singh BP, Agrawal S. Determination of Embelin from Embelia ribes by high performance thin layer chromatography. Indian Drugs. 1999; 36: 41-43.

Yıl 2018, Cilt: 22 Sayı: 1, 66 - 73, 27.06.2025

Firoj Tamboli , Vinod D. Rangari Suresh G. Killedar Sagar U. Jadhav Trupti S. Ghatage Vishwajeet P. Kore

Öz

Kaynakça

  • [1] Dey KK. An Introduction to Plant Tissue Culture, New Central Book Agency (P) Ltd., Calcutta, India, 1997, pp. 35-44.
  • [2] Narayanswamy S. Plant Cell and Tissue Culture, Tata McGraw-Hill Education, New Delhi, India, 1994, pp. 22-51.
  • [3] Chatterji N, Rastogi RP, Dhar ML. Chemical examination of Bacopa monnieria Wettst: Part 1 – Isolation of chemical constituents. Indian J Chem. 1963; 1: 212-215.
  • [4] Asolkar LV, Kakkar KK, Chakre OS. Second Supplement to Glossary of Indian Medicinal Plants with Active Principles (Part I), CSIR, New Delhi, India, 1992, pp. 112-113.
  • [5] Nadkarni KM. The Indian Materia Medica I, Popular Prakashan, Mumbai, India, 1982, pp. 624-625.
  • [6] Sala AV. Indian Medicinal Plants, Orient Longman, Kottakkal, India, 1994, pp. 235-236.
  • [7] Mishra SK, Tiwari KN, Shivna PL, Mishra AK. Micropropagation and comparative phytochemical, antioxidant study of Bacopa monnieri (L.) Pennell. Res J Pharm Biol Chem Sci. 2015; 6(6): 902-912.
  • [8] Ambasta SP. Wealth of India Raw Materials, Vol. 2:B. Council of Scientific and Industrial Research, New Delhi, India, 1988, pp. 23-24.
  • [9] Jain SK, DeFilipps RA. Medicinal Plants of India, Vol. 2. Reference Publication Inc., Michigan, USA, 1991, pp. 556-557.
  • [10] Soundararajan T, Karrunakaran CM. Micropropagation of Bacopa monnieri through protoplast. Asian J Biotechnol. 2011; 3(2): 135-152.
  • [11] Heble MR. Production of secondary metabolites through tissue culture and its prospects for commercial use. In: Islam AS, editor. Plant Tissue Culture, Oxford and IBH Publishing Company Pvt. Ltd., New Delhi, 1997, pp. 161-168.
  • [12] George J, Ravishankar GA. Harnessing high value metabolites from plant cells. Ind J Pharm Educ. 1996; 30: 120-130.
  • [13] Fowler HW. Extraction. In: Carter SJ, editor. Cooper and Gunn’s Tutorial Pharmacy, 6th ed. CBS Publishers and Distributors, New Delhi, 1986, pp. 251-261.
  • [14] Yelne MB, Borkar GB, Sharma PC. In vitro propagation of Brahmi – Bacopa monnieri (L.) Pennell. Bull Medico-Ethno-Bot Res. 1997; 18(3-4): 45-50.
  • [15] Stahl E. Thin Layer Chromatography, 2nd ed., Springer-Verlag, Berlin Heidelberg, Germany, 1969, pp. 904-905.
  • [16] Srivastava VK, Srivastava KK. Introduction to Chromatography, S. Chand and Company Ltd., Delhi, India, 1991, pp. 8-32.
  • [17] Sethi PD. High-performance Thin Layer Chromatography, CBS Publisher and Distributors, New Delhi, India, 1996, pp. 4-28.
  • [18] Narayana DBA, Agarwal S, Luthra SK, Srinivas NS. A HPTLC method for the quantitative analysis of vasicine in Adhatoda vasica. Indian Drugs. 1995; 32: 583-586.
  • [19] Chauhan SK, Singh BP, Agrawal S. Determination of Embelin from Embelia ribes by high performance thin layer chromatography. Indian Drugs. 1999; 36: 41-43.
Toplam 19 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Farmakognozi
Bölüm Articles
Yazarlar

Firoj Tamboli 0000-0002-5809-6303

Vinod D. Rangari 0000-0003-2784-6973

Suresh G. Killedar 0000-0001-7017-1997

Sagar U. Jadhav

Trupti S. Ghatage 0000-0003-1225-7746

Vishwajeet P. Kore 0000-0002-9377-9679

Yayımlanma Tarihi 27 Haziran 2025
Yayımlandığı Sayı Yıl 2018 Cilt: 22 Sayı: 1

Kaynak Göster

APA Tamboli, F., D. Rangari, V., G. Killedar, S., U. Jadhav, S., vd. (2025). Comparative phytochemical evaluation of natural and micropropagated plants of Bacopa monnieri (L.). Journal of Research in Pharmacy, 22(1), 66-73.
AMA Tamboli F, D. Rangari V, G. Killedar S, U. Jadhav S, S. Ghatage T, P. Kore V. Comparative phytochemical evaluation of natural and micropropagated plants of Bacopa monnieri (L.). J. Res. Pharm. Haziran 2025;22(1):66-73.
Chicago Tamboli, Firoj, Vinod D. Rangari, Suresh G. Killedar, Sagar U. Jadhav, Trupti S. Ghatage, ve Vishwajeet P. Kore. “Comparative Phytochemical Evaluation of Natural and Micropropagated Plants of Bacopa Monnieri (L.)”. Journal of Research in Pharmacy 22, sy. 1 (Haziran 2025): 66-73.
EndNote Tamboli F, D. Rangari V, G. Killedar S, U. Jadhav S, S. Ghatage T, P. Kore V (01 Haziran 2025) Comparative phytochemical evaluation of natural and micropropagated plants of Bacopa monnieri (L.). Journal of Research in Pharmacy 22 1 66–73.
IEEE F. Tamboli, V. D. Rangari, S. G. Killedar, S. U. Jadhav, T. S. Ghatage, ve V. P. Kore, “Comparative phytochemical evaluation of natural and micropropagated plants of Bacopa monnieri (L.)”, J. Res. Pharm., c. 22, sy. 1, ss. 66–73, 2025.
ISNAD Tamboli, Firoj vd. “Comparative Phytochemical Evaluation of Natural and Micropropagated Plants of Bacopa Monnieri (L.)”. Journal of Research in Pharmacy 22/1 (Haziran 2025), 66-73.
JAMA Tamboli F, D. Rangari V, G. Killedar S, U. Jadhav S, S. Ghatage T, P. Kore V. Comparative phytochemical evaluation of natural and micropropagated plants of Bacopa monnieri (L.). J. Res. Pharm. 2025;22:66–73.
MLA Tamboli, Firoj vd. “Comparative Phytochemical Evaluation of Natural and Micropropagated Plants of Bacopa Monnieri (L.)”. Journal of Research in Pharmacy, c. 22, sy. 1, 2025, ss. 66-73.
Vancouver Tamboli F, D. Rangari V, G. Killedar S, U. Jadhav S, S. Ghatage T, P. Kore V. Comparative phytochemical evaluation of natural and micropropagated plants of Bacopa monnieri (L.). J. Res. Pharm. 2025;22(1):66-73.