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Determination of antioxidant activities of solvent extracts from an endemic plant: Phlomis leucophracta

Yıl 2018, Cilt: 22 Sayı: 1, 86 - 90, 27.06.2025

Cengiz Sarıkürkçü , Mustafa Cengiz , Ahmet Küçükyumru Gökhan Zengin

Öz

The members of the genus Phlomis have been traditionally used for therapeutic purposes in Turkey. In this study, the antioxidant properties of different extracts from P. leucophracta were investigated. Antioxidant properties were evaluated by different assays including free radical scavenging (DPPH assay), reducing power (potassium ferricyanide method), β-carotene/linoleic acid, metal chelating and phosphomolybdenum. Moreover, total phenolic and flavonoid contents were detected for each extracts. Total phenolic and flavonoid contents were detected as 30.86-55.00 mg GAE/g extract and 4.93-26.09 mg QE/g extract, respectively. The methanol and water extracts exhibited higher DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging and reducing power abilities as compared to ethyl acetate and hexane extracts. The best activity was observed by the hexane extract in β-carotene/linoleic acid assay (94.35% at 2 mg/mL). In metal chelating ability, those samples exhibited the following order (at 0.25 mg/mL concentration): Water (73.90%)>Hexane(64.87%)>Ethyl acetate(4.88%)>Methanol (2.28%). Based on our results, P. leucophracta may be utilized as a natural source of antioxidant compounds in food and pharmaceutical areas.

Anahtar Kelimeler

Phlomis leucophracta phenolics DPPH antioxidant activity reducing power

Kaynakça

  • [1] Huang W-Y, Cai Y-Z, Zhang Y. Natural phenolic compounds from medicinal herbs and dietary plants: potential use for cancer prevention. Nutr Cancer. 2009;62(1):1-20.
  • [2] Silva N, Fernandes Júnior A. Biological properties of medicinal plants: a review of their antimicrobial activity. J Venom Anim Toxins Incl Trop Dis. 2010;16(3):402-413.
  • [3] Lin D, Xiao M, Zhao J, Li Z, Xing B, Li X, Kong M, Li L, Zhang Q, Liu Y. An overview of plant phenolic compounds and their importance in human nutrition and management of type 2 diabetes. Molecules. 2016;21(10):1374.
  • [4] Locatelli M, Zengin G, Uysal A, Carradori S, De Luca E, Bellagamba G, Aktumsek A, Lazarova I. Multicomponent pattern and biological activities of seven Asphodeline taxa: potential sources of natural-functional ingredients for bioactive formulations. J Enzyme Inhib Med Chem. 2017;32(1):60-67.
  • [5] Mollica A, Locatelli M, Macedonio G, Carradori S, Sobolev AP, De Salvador RF, Monti SM, Buonanno M, Zengin G, Angeli A. Microwave-assisted extraction, HPLC analysis, and inhibitory effects on carbonic anhydrase isoforms of 14 blueberry Italian cultivars. J Enzyme Inhib Med Chem. 2016;31(sup4):1-6.
  • [6] Paduch R, Wiater A, Locatelli M, Tomczyk M. Aqueous extracts of selected Potentilla species modulate biological activity of human normal colon cells. Curr Drug Targets. 2015;16(13):1495-1502.
  • [7] Zengin G, Menghini L, Malatesta L, De Luca E, Bellagamba G, Uysal S, Aktumsek A, Locatelli M. Comparative study of biological activities and multicomponent pattern of two wild Turkish species: Asphodeline anatolica and Potentilla speciosa. J Enzyme Inhib Med Chem. 2016;31(sup1):203-208.
  • [8] Baytop T. Therapy with medicinal plants in Turkey (Past and Present). Istanbul: Nobel Tip Basimevi; 1999.
  • [9] Demirci S, Özhatay N. An ethnobotanical study in Kahramanmaraş (Turkey); wild plants used for medicinal purpose in Andirin, Kahramanmaraş. Turk J Pharm Sci. 2012;9(1):75-92.
  • [10] Li MX, Shang XF, Jia ZP, Zhang RX. Phytochemical and biological studies of plants from the genus Phlomis. Chem Biodivers. 2010;7(2):283-301.
  • [11] Limem-Ben Amor I, Boubaker J, Sgaier MB, Skandrani I, Bhouri W, Neffati A, Kilani S, Bouhlel I, Ghedira K, Chekir-Ghedira L. Phytochemistry and biological activities of Phlomis species. J Ethnopharmacol. 2009;125(2):183-202.
  • [12] Tuzlacı E, Erol M. Turkish folk medicinal plants. Part II: Eğirdir (Isparta). Fitoterapia. 1999;70(6):593-610.
  • [13] Gürdal B, Kültür Ş. An ethnobotanical study of medicinal plants in Marmaris (Muğla, Turkey). J Ethnopharmacol. 2013;146(1):113-126.
  • [14] Delnavazi M, Mohammadifar F, Rustaie A, Aghaahmadi M, Yassa N. Phytochemical constituents, antioxidant activity and toxicity potential of Phlomis olivieri Benth. Res J Pharm. 2016;3(2):9-15.
  • [15] Sarikurkcu C, Sabih Ozer M, Cakir A, Eskici M, Mete E. GC/MS evaluation and in vitro antioxidant activity of essential oil and solvent extracts of an endemic plant: Phlomis bourgaei Boiss. Evid Based Complement Alternat Med. 2013;2013:293080.
  • [16] Sarikurkcu C, Uren MC, Kocak MS, Cengiz M, Tepe B. Chemical composition, antioxidant, and enzyme inhibitory activities of the essential oils of three Phlomis species. Food Sci Biotechnol. 2016;25(3):687-693.
  • [17] Sarikurkcu C, Uren MC, Tepe B, Cengiz M, Kocak MS. Phenolic content, enzyme inhibitory and antioxidative activity potentials of Phlomis nissolii and P. pungens var. pungens. Ind Crops Prod. 2014;62:333-340.
  • [18] Sarikurkcu C, Uren MC, Tepe B, Cengiz M, Kocak MS. Phlomis armeniaca: Phenolic compounds, enzyme inhibitory and antioxidant activities. Ind Crops Prod. 2015;78:95-101.
  • [19] Sobeh M, Mamadalieva NZ, Mohamed T, Krstin S, Youssef FS, Ashour ML, Azimova SS, Wink M. Chemical profiling of Phlomis thapsoides and in vitro testing of its biological activities. J Med Chem. 2016;25(10):2304-2315.
  • [20] Uysal A, Gunes E, Sarikurkcu C, Celik H, Durak Y, Uren MC. New prospective materials for chemoprevention: Three Phlomis species. Brit J Pharm Res. 2016;10(3).
  • [21] Li H, Zhang D, Tan L-H, Yu B, Zhao S-P, Cao W-G. Comparison of antioxidant properties of solvent extracts from Dipsacus asperoides by LC-ESI-QTOF-MS–MS. S Afr J Bot. 2017;109:1-8.
  • [22] Sumczynski D, Kotásková E, Orsavová J, Valášek P. Contribution of phenolics to antioxidant activity and in vitro digestibility of wild rices (Zizania aquatica L.). Food Chem. 2017;218:107-115.
  • [23] Chen W, Zhao J, Bao T, Xie J, Liang W, Gowd V. Comparative study on phenolics and antioxidant property of bayberry cultivars. J Funct Foods. 2016;27:472-482.
  • [24] Sarikurkcu C, Kocak MS, Tepe B, Uren MC. An alternative antioxidative and enzyme inhibitory agent from Turkey: Robinia pseudoacacia L. Ind Crops Prod. 2015;78:110-115.
  • [25] Zengin G, Sarikurkcu C, Aktumsek A, Ceylan R. Sideritis galatica Bornm.: A source of multifunctional agents for oxidative damage, Alzheimer's and diabetes management. J Funct Foods. 2014;11:538-547.
  • [26] Ceylan R, Katanić J, Zengin G, Matić S, Aktumsek A, Boroja T, Stanić S, Mihailović V, Guler GO, Boga M. Chemical and biological fingerprints of Cytisopsis dorycniifolia and Ebenus hirsuta. Ind Crops Prod. 2016;84:254-262.
  • [27] Marathe SA, Rajalakshmi V, Jamdar SN, Sharma A. Comparative study on antioxidant activity of Indian legumes. Food Chem Toxicol. 2011;49(9):2005-2012.
  • [28] Wang T, Jonsdottir R, Ólafsdóttir G. Total phenolic compounds, radical scavenging and metal chelation of Icelandic seaweeds. Food Chem. 2009;116(1):240-248.
  • [29] Sarikurkcu C. Antioxidant activities of extracts from endemic Cyclamen mirabile Hildebr. Afr J Biotechnol. 2011;10(5):831-839.
  • [30] Oyaizu M. Studies on browning reaction products. Jpn J Nutr Diet. 1986;44(6):307-315.

Yıl 2018, Cilt: 22 Sayı: 1, 86 - 90, 27.06.2025

Cengiz Sarıkürkçü , Mustafa Cengiz , Ahmet Küçükyumru Gökhan Zengin

Öz

Kaynakça

  • [1] Huang W-Y, Cai Y-Z, Zhang Y. Natural phenolic compounds from medicinal herbs and dietary plants: potential use for cancer prevention. Nutr Cancer. 2009;62(1):1-20.
  • [2] Silva N, Fernandes Júnior A. Biological properties of medicinal plants: a review of their antimicrobial activity. J Venom Anim Toxins Incl Trop Dis. 2010;16(3):402-413.
  • [3] Lin D, Xiao M, Zhao J, Li Z, Xing B, Li X, Kong M, Li L, Zhang Q, Liu Y. An overview of plant phenolic compounds and their importance in human nutrition and management of type 2 diabetes. Molecules. 2016;21(10):1374.
  • [4] Locatelli M, Zengin G, Uysal A, Carradori S, De Luca E, Bellagamba G, Aktumsek A, Lazarova I. Multicomponent pattern and biological activities of seven Asphodeline taxa: potential sources of natural-functional ingredients for bioactive formulations. J Enzyme Inhib Med Chem. 2017;32(1):60-67.
  • [5] Mollica A, Locatelli M, Macedonio G, Carradori S, Sobolev AP, De Salvador RF, Monti SM, Buonanno M, Zengin G, Angeli A. Microwave-assisted extraction, HPLC analysis, and inhibitory effects on carbonic anhydrase isoforms of 14 blueberry Italian cultivars. J Enzyme Inhib Med Chem. 2016;31(sup4):1-6.
  • [6] Paduch R, Wiater A, Locatelli M, Tomczyk M. Aqueous extracts of selected Potentilla species modulate biological activity of human normal colon cells. Curr Drug Targets. 2015;16(13):1495-1502.
  • [7] Zengin G, Menghini L, Malatesta L, De Luca E, Bellagamba G, Uysal S, Aktumsek A, Locatelli M. Comparative study of biological activities and multicomponent pattern of two wild Turkish species: Asphodeline anatolica and Potentilla speciosa. J Enzyme Inhib Med Chem. 2016;31(sup1):203-208.
  • [8] Baytop T. Therapy with medicinal plants in Turkey (Past and Present). Istanbul: Nobel Tip Basimevi; 1999.
  • [9] Demirci S, Özhatay N. An ethnobotanical study in Kahramanmaraş (Turkey); wild plants used for medicinal purpose in Andirin, Kahramanmaraş. Turk J Pharm Sci. 2012;9(1):75-92.
  • [10] Li MX, Shang XF, Jia ZP, Zhang RX. Phytochemical and biological studies of plants from the genus Phlomis. Chem Biodivers. 2010;7(2):283-301.
  • [11] Limem-Ben Amor I, Boubaker J, Sgaier MB, Skandrani I, Bhouri W, Neffati A, Kilani S, Bouhlel I, Ghedira K, Chekir-Ghedira L. Phytochemistry and biological activities of Phlomis species. J Ethnopharmacol. 2009;125(2):183-202.
  • [12] Tuzlacı E, Erol M. Turkish folk medicinal plants. Part II: Eğirdir (Isparta). Fitoterapia. 1999;70(6):593-610.
  • [13] Gürdal B, Kültür Ş. An ethnobotanical study of medicinal plants in Marmaris (Muğla, Turkey). J Ethnopharmacol. 2013;146(1):113-126.
  • [14] Delnavazi M, Mohammadifar F, Rustaie A, Aghaahmadi M, Yassa N. Phytochemical constituents, antioxidant activity and toxicity potential of Phlomis olivieri Benth. Res J Pharm. 2016;3(2):9-15.
  • [15] Sarikurkcu C, Sabih Ozer M, Cakir A, Eskici M, Mete E. GC/MS evaluation and in vitro antioxidant activity of essential oil and solvent extracts of an endemic plant: Phlomis bourgaei Boiss. Evid Based Complement Alternat Med. 2013;2013:293080.
  • [16] Sarikurkcu C, Uren MC, Kocak MS, Cengiz M, Tepe B. Chemical composition, antioxidant, and enzyme inhibitory activities of the essential oils of three Phlomis species. Food Sci Biotechnol. 2016;25(3):687-693.
  • [17] Sarikurkcu C, Uren MC, Tepe B, Cengiz M, Kocak MS. Phenolic content, enzyme inhibitory and antioxidative activity potentials of Phlomis nissolii and P. pungens var. pungens. Ind Crops Prod. 2014;62:333-340.
  • [18] Sarikurkcu C, Uren MC, Tepe B, Cengiz M, Kocak MS. Phlomis armeniaca: Phenolic compounds, enzyme inhibitory and antioxidant activities. Ind Crops Prod. 2015;78:95-101.
  • [19] Sobeh M, Mamadalieva NZ, Mohamed T, Krstin S, Youssef FS, Ashour ML, Azimova SS, Wink M. Chemical profiling of Phlomis thapsoides and in vitro testing of its biological activities. J Med Chem. 2016;25(10):2304-2315.
  • [20] Uysal A, Gunes E, Sarikurkcu C, Celik H, Durak Y, Uren MC. New prospective materials for chemoprevention: Three Phlomis species. Brit J Pharm Res. 2016;10(3).
  • [21] Li H, Zhang D, Tan L-H, Yu B, Zhao S-P, Cao W-G. Comparison of antioxidant properties of solvent extracts from Dipsacus asperoides by LC-ESI-QTOF-MS–MS. S Afr J Bot. 2017;109:1-8.
  • [22] Sumczynski D, Kotásková E, Orsavová J, Valášek P. Contribution of phenolics to antioxidant activity and in vitro digestibility of wild rices (Zizania aquatica L.). Food Chem. 2017;218:107-115.
  • [23] Chen W, Zhao J, Bao T, Xie J, Liang W, Gowd V. Comparative study on phenolics and antioxidant property of bayberry cultivars. J Funct Foods. 2016;27:472-482.
  • [24] Sarikurkcu C, Kocak MS, Tepe B, Uren MC. An alternative antioxidative and enzyme inhibitory agent from Turkey: Robinia pseudoacacia L. Ind Crops Prod. 2015;78:110-115.
  • [25] Zengin G, Sarikurkcu C, Aktumsek A, Ceylan R. Sideritis galatica Bornm.: A source of multifunctional agents for oxidative damage, Alzheimer's and diabetes management. J Funct Foods. 2014;11:538-547.
  • [26] Ceylan R, Katanić J, Zengin G, Matić S, Aktumsek A, Boroja T, Stanić S, Mihailović V, Guler GO, Boga M. Chemical and biological fingerprints of Cytisopsis dorycniifolia and Ebenus hirsuta. Ind Crops Prod. 2016;84:254-262.
  • [27] Marathe SA, Rajalakshmi V, Jamdar SN, Sharma A. Comparative study on antioxidant activity of Indian legumes. Food Chem Toxicol. 2011;49(9):2005-2012.
  • [28] Wang T, Jonsdottir R, Ólafsdóttir G. Total phenolic compounds, radical scavenging and metal chelation of Icelandic seaweeds. Food Chem. 2009;116(1):240-248.
  • [29] Sarikurkcu C. Antioxidant activities of extracts from endemic Cyclamen mirabile Hildebr. Afr J Biotechnol. 2011;10(5):831-839.
  • [30] Oyaizu M. Studies on browning reaction products. Jpn J Nutr Diet. 1986;44(6):307-315.
Toplam 30 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Eczacılıkta Analitik Kimya, Farmakognozi
Bölüm Articles
Yazarlar

Cengiz Sarıkürkçü 0000-0001-5094-2520

Mustafa Cengiz

Ahmet Küçükyumru

Gökhan Zengin 0000-0001-6548-7823

Yayımlanma Tarihi 27 Haziran 2025
Yayımlandığı Sayı Yıl 2018 Cilt: 22 Sayı: 1

Kaynak Göster

APA Sarıkürkçü, C., Cengiz, M., Küçükyumru, A., Zengin, G. (2025). Determination of antioxidant activities of solvent extracts from an endemic plant: Phlomis leucophracta. Journal of Research in Pharmacy, 22(1), 86-90.
AMA Sarıkürkçü C, Cengiz M, Küçükyumru A, Zengin G. Determination of antioxidant activities of solvent extracts from an endemic plant: Phlomis leucophracta. J. Res. Pharm. Haziran 2025;22(1):86-90.
Chicago Sarıkürkçü, Cengiz, Mustafa Cengiz, Ahmet Küçükyumru, ve Gökhan Zengin. “Determination of Antioxidant Activities of Solvent Extracts from an Endemic Plant: Phlomis Leucophracta”. Journal of Research in Pharmacy 22, sy. 1 (Haziran 2025): 86-90.
EndNote Sarıkürkçü C, Cengiz M, Küçükyumru A, Zengin G (01 Haziran 2025) Determination of antioxidant activities of solvent extracts from an endemic plant: Phlomis leucophracta. Journal of Research in Pharmacy 22 1 86–90.
IEEE C. Sarıkürkçü, M. Cengiz, A. Küçükyumru, ve G. Zengin, “Determination of antioxidant activities of solvent extracts from an endemic plant: Phlomis leucophracta”, J. Res. Pharm., c. 22, sy. 1, ss. 86–90, 2025.
ISNAD Sarıkürkçü, Cengiz vd. “Determination of Antioxidant Activities of Solvent Extracts from an Endemic Plant: Phlomis Leucophracta”. Journal of Research in Pharmacy 22/1 (Haziran 2025), 86-90.
JAMA Sarıkürkçü C, Cengiz M, Küçükyumru A, Zengin G. Determination of antioxidant activities of solvent extracts from an endemic plant: Phlomis leucophracta. J. Res. Pharm. 2025;22:86–90.
MLA Sarıkürkçü, Cengiz vd. “Determination of Antioxidant Activities of Solvent Extracts from an Endemic Plant: Phlomis Leucophracta”. Journal of Research in Pharmacy, c. 22, sy. 1, 2025, ss. 86-90.
Vancouver Sarıkürkçü C, Cengiz M, Küçükyumru A, Zengin G. Determination of antioxidant activities of solvent extracts from an endemic plant: Phlomis leucophracta. J. Res. Pharm. 2025;22(1):86-90.