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Volatile constituents and antimicrobial activity of Vinca major L. subsp. hirsuta (Boiss) stearn grown in Turkey

Yıl 2021, Cilt: 25 Sayı: 5, 581 - 588, 28.06.2025

İshak Erik , Gözde Kilic Büşra Korkmaz , Seda Fandakli , Şengül Alpay Karaoğlu , Nurettin Yaylı

Öz

In this study, volatile compounds (VCs) in the essential oil (EO), SPME and SPME of n-hexane extract of Vinca major subsp. hirsuta (Boiss.) Stearn were analyzed by GC-FID/MS instrument. A total of 32, 38, and 26 compounds with in the 98.5%, 98.6%, and 98.6% were identified, respectively. As a result of VCs study, (Z)-3-hexenol (36.8%) in the EO, 1,3,5-trimethylbenzene (31,5%) in the SPME, and phenylethyl alcohol (32.8 %) in the SPME of n-hexane extract of V. major subsp. hirsuta were found to be major compounds. Sesquiterpenes (20.6%, and 11.5%) for the HD and SPME, monoterpenes (18.6%) for the SPME of n-hexane extract of V. major subsp. hirsuta were found as the main groups among the terpenic compounds, respectively. Then, the antimicrobial activity of the EO and the solvent extracts (n-hexane, acetonitrile, methanol, and water) of V. major subs. hirsuta against 3 gram negative, 3 gram positive, 1 tuberculosis and 2 fungus were screened. The EO showed the only activity against the Mycobacterium smegmatis ATCC607 (MIC, 152 µg/mL) and Candida albicans ATCC60193 (MIC, 38 µg/mL). The n-hexane extract did not show any activity against all tested microorganisms. The best antimicrobial activity for the acetonitrile, methanol, and water extracts were observed against M. smegmatis with 69 µg/mL, 609 µg/mL, and 437 µg/mL MIC values, respectively. None of the extracts were found to be active to Enterococcus faecalis ATCC29212, Staphylococcus aureus ATCC25923, and Saccharomyces cerevisiae RSKK 251.

Anahtar Kelimeler

Vinca major subsp. hirsuta Volatile constituents Antimicrobial activity SPME GC-FID/MS

Kaynakça

  • [1] Wagstaff S. A phylogenetic interpretation of pollen morphology in tribe Mentheae (Labiatae). Advances in Labiatae Science Royal Botanic Gardens, Kew. 1992, pp.113-124.
  • [2] Chamberline DF. In: Davis PH. (Eds.). Flora of Turkey and East Aegean Islands. Edinburgh University Press, Edinburgh, 1978, pp.161-163.
  • [3] Baytop T. Treatment Plant in Turkey (Past and Present). Istanbul University Press, Turkey, Istanbul, 1984.
  • [4] Meshram GA, Khamkar SS, Metangale GS, Kulkarni TS. Characterization of campesterol, stigmasterol and β- sitosterol from Vinca rosealeaves by GC-MS and biological evaluation world. J Pharm Res. 2014; 3(4).
  • [5] Logan BA, Demmig-Adams B, Adams WW, Grace SC. Antioxidants and xanthophyll cycle-dependent energy dissipation in Cucurbita pepo L. and Vinca major L. acclimated to four growth PPFDs in the field. J Exp Bot. 1998; 49(328): 1869-1879.
  • [6] Yadao N, Priya CL, Rao KVB. Carbohydrate hydrolyzing enzyme inhibitor property, antioxidant, and phytochemical analysis of Cassia auriculata, Delonix regia and Vinca rosea Linn: an in vitro study. J Appl Pharm Sci. 2015; 5(05): 18-27.
  • [7] Evans WC. Trease and Evans Pharmacognosy. Nottingham University Press, Nottingham, 2002, pp. 21.
  • [8] Rajput MS, Nair V, Chauhan A, Jawanjal H, Dange V. Evaluation of antidiarrheal activity of aerial parts of Vinca major in experimental animals. Middle East J Sci Res. 2011; 7(5): 784-788.
  • [9] Oliveros-Belardo L, Coronel V, Nicolas EC, Villarica AS. Some constituents of the volatile oil of the leaves of Lochnera rosea [Vinca rosea]. Perf and Essent Oil Rec. 1965; 56(4): 214-216.
  • [10] Aldaba L, Oliveros-Belardo L. Preliminary chemical study of the leaves of the Philippine variety of Lochnera (Vinca) rosea Reichb. Rev Filip Med. 1938; 29: 259-293.
  • [11] George M, Joseph L, Singh B, Sabu NS. Genotoxicity study of Vinca difformis using rat bone marrow. Int J Pharm Phytopharm Res. 2016; 5(1): 108-113.
  • [12] Leylaie S, Zafari D. Antiproliferative and antimicrobial activities of secondary metabolites and phylogenetic study of endophytic Trichoderma species from Vinca plants. Front Microbiol. 2018; 9: 1484.
  • [13]Saral Ö, Şahin H, Karaköse M. Determination of antioxidant activity and phenolic compounds in Vinca major subsp. hirsuta by RP-HPLC-UV. ACU J For Fac. 2015; 16(2): 124-131.
  • [14] Yayli N, Fandakli S, Korkmaz B, Barut B, Renda G, Erik I. Biological evaluation (antimicrobial, antioxidant, and enzyme inhibitions), total phenolic content and volatile chemical compositions of Caucasalia macrophylla (M. Bieb.) B. Nord.(Asteraceae). J Essent Oil-Bear Plants. 2018; 21(5): 1359-1373.
  • [15] Erik İ, Kılıç G, Öztürk E, Karaoğlu ŞA, Yaylı N. Chemical composition, antimicrobial, and lipase enzyme activity of essential oil and solvent extracts from Serapias orientalis subsp. orientalis. Turk J Chem. 2020; 44(6): 1655-1662.
  • [16] Adams RP. Identification of Essential Oil Components by Gas Chromatography/mass Spectrometry. Allured Publishing Corporation Carol Stream, IL; 2007.
  • [17] Yayli N, Yaşar A, Güleç C, Usta A, Kolaylı S, Coşkunçelebi K, Karaoğlu ŞA. Composition and antimicrobial activity of essential oils from Centaurea sessilis and Centaurea armena. Phytochemistry. 2005; 66(14): 1741-1745.
  • [18] Usta A, Yayli B, Kahrinman N, Karaoglu Ş, Yayli N. Composition and antimicrobial activity of essential oil from the flower of Rhododendron luteum Sweet. Asian J Chem. 2012; 24(5): 1927-1930.
  • [19] Kahriman N, Tosun G, İskender NY, Karaoğlu ŞA, Yayli N. Antimicrobial activity and a comparative essential oil analysis of Centaurea pulcherrima Willd. var. pulcherrima extracted by hydrodistillation and microwave distillation. Nat Prod Res. 2012; 26(8): 703-712.
  • [20] Çelik G, Kılıç G, Kanbolat Ş, Şener SÖ, Karaköse M, Yaylı N, Karaoğlu ŞA. Biological activity, and volatile and phenolic compounds from five Lamiaceae species. Flavour Fragr J. 2021; 36: 223-232.
  • [21] Renda G, Özel A, Barut B, Korkmaz B, Yayli N. The volatile chemical compositions of the essential Oil/SPME and enzyme inhibitory and radical scavenging activities of solvent extracts and the essential oils from Coronilla orientalis Miller and C. varia L. grows in Turkey. Iran J Pharm Sci. 2019; 18(4): 1831–1842.
  • [22] Colak NU, Yıldırım S, Bozdeveci A, Yaylı N, Çoşkunçelebi K, Fandaklı S, Yaşar A. Essential oil composition, antimicrobial and antioxidant activities of Salvia staminea. Rec Nat Prod. 2018; 12: 86-94.
  • [23] Barry AL. Standards NCCLS. Methods for determining bactericidal activity of antimicrobial agents: approved guideline. National Committee for Clinical Laboratory Standards Wayne, PA, 1999.
  • [24] Woods GL, Brown-Elliott BA, Desmond EP, Hall GS, Heifets LB, Pfyffer GE. Ridderhof JC, Wallace RJJ, Warren NG, Witebsky FG. Susceptibility testing of mycobacteria, nocardiae, and other aerobic actinomycetes; approved standard, NCCLS, 2010.
  • [25] Şimşek Sezer EN, Uysal T. Volatile and phenolic compositions of the leaves of two Vinca L. species from Turkey. Curr Pers MAPs. 2018; 1(2): 103-110.
  • [26] Ashrafi B, Ramak P, Ezatpour B, Talei RG. Biological activity and chemical composition of the essential oil of Nepeta cataria L. J Res Pharm. 2019; 23(2): 336-343.
  • [27] İşcan G, Demirci B, Köse YB. Antimicrobial essential oil of Origanum boissieri Ietswaart. J Res Pharm. 2020; 24(2): 233- 239.

Yıl 2021, Cilt: 25 Sayı: 5, 581 - 588, 28.06.2025

İshak Erik , Gözde Kilic Büşra Korkmaz , Seda Fandakli , Şengül Alpay Karaoğlu , Nurettin Yaylı

Öz

Kaynakça

  • [1] Wagstaff S. A phylogenetic interpretation of pollen morphology in tribe Mentheae (Labiatae). Advances in Labiatae Science Royal Botanic Gardens, Kew. 1992, pp.113-124.
  • [2] Chamberline DF. In: Davis PH. (Eds.). Flora of Turkey and East Aegean Islands. Edinburgh University Press, Edinburgh, 1978, pp.161-163.
  • [3] Baytop T. Treatment Plant in Turkey (Past and Present). Istanbul University Press, Turkey, Istanbul, 1984.
  • [4] Meshram GA, Khamkar SS, Metangale GS, Kulkarni TS. Characterization of campesterol, stigmasterol and β- sitosterol from Vinca rosealeaves by GC-MS and biological evaluation world. J Pharm Res. 2014; 3(4).
  • [5] Logan BA, Demmig-Adams B, Adams WW, Grace SC. Antioxidants and xanthophyll cycle-dependent energy dissipation in Cucurbita pepo L. and Vinca major L. acclimated to four growth PPFDs in the field. J Exp Bot. 1998; 49(328): 1869-1879.
  • [6] Yadao N, Priya CL, Rao KVB. Carbohydrate hydrolyzing enzyme inhibitor property, antioxidant, and phytochemical analysis of Cassia auriculata, Delonix regia and Vinca rosea Linn: an in vitro study. J Appl Pharm Sci. 2015; 5(05): 18-27.
  • [7] Evans WC. Trease and Evans Pharmacognosy. Nottingham University Press, Nottingham, 2002, pp. 21.
  • [8] Rajput MS, Nair V, Chauhan A, Jawanjal H, Dange V. Evaluation of antidiarrheal activity of aerial parts of Vinca major in experimental animals. Middle East J Sci Res. 2011; 7(5): 784-788.
  • [9] Oliveros-Belardo L, Coronel V, Nicolas EC, Villarica AS. Some constituents of the volatile oil of the leaves of Lochnera rosea [Vinca rosea]. Perf and Essent Oil Rec. 1965; 56(4): 214-216.
  • [10] Aldaba L, Oliveros-Belardo L. Preliminary chemical study of the leaves of the Philippine variety of Lochnera (Vinca) rosea Reichb. Rev Filip Med. 1938; 29: 259-293.
  • [11] George M, Joseph L, Singh B, Sabu NS. Genotoxicity study of Vinca difformis using rat bone marrow. Int J Pharm Phytopharm Res. 2016; 5(1): 108-113.
  • [12] Leylaie S, Zafari D. Antiproliferative and antimicrobial activities of secondary metabolites and phylogenetic study of endophytic Trichoderma species from Vinca plants. Front Microbiol. 2018; 9: 1484.
  • [13]Saral Ö, Şahin H, Karaköse M. Determination of antioxidant activity and phenolic compounds in Vinca major subsp. hirsuta by RP-HPLC-UV. ACU J For Fac. 2015; 16(2): 124-131.
  • [14] Yayli N, Fandakli S, Korkmaz B, Barut B, Renda G, Erik I. Biological evaluation (antimicrobial, antioxidant, and enzyme inhibitions), total phenolic content and volatile chemical compositions of Caucasalia macrophylla (M. Bieb.) B. Nord.(Asteraceae). J Essent Oil-Bear Plants. 2018; 21(5): 1359-1373.
  • [15] Erik İ, Kılıç G, Öztürk E, Karaoğlu ŞA, Yaylı N. Chemical composition, antimicrobial, and lipase enzyme activity of essential oil and solvent extracts from Serapias orientalis subsp. orientalis. Turk J Chem. 2020; 44(6): 1655-1662.
  • [16] Adams RP. Identification of Essential Oil Components by Gas Chromatography/mass Spectrometry. Allured Publishing Corporation Carol Stream, IL; 2007.
  • [17] Yayli N, Yaşar A, Güleç C, Usta A, Kolaylı S, Coşkunçelebi K, Karaoğlu ŞA. Composition and antimicrobial activity of essential oils from Centaurea sessilis and Centaurea armena. Phytochemistry. 2005; 66(14): 1741-1745.
  • [18] Usta A, Yayli B, Kahrinman N, Karaoglu Ş, Yayli N. Composition and antimicrobial activity of essential oil from the flower of Rhododendron luteum Sweet. Asian J Chem. 2012; 24(5): 1927-1930.
  • [19] Kahriman N, Tosun G, İskender NY, Karaoğlu ŞA, Yayli N. Antimicrobial activity and a comparative essential oil analysis of Centaurea pulcherrima Willd. var. pulcherrima extracted by hydrodistillation and microwave distillation. Nat Prod Res. 2012; 26(8): 703-712.
  • [20] Çelik G, Kılıç G, Kanbolat Ş, Şener SÖ, Karaköse M, Yaylı N, Karaoğlu ŞA. Biological activity, and volatile and phenolic compounds from five Lamiaceae species. Flavour Fragr J. 2021; 36: 223-232.
  • [21] Renda G, Özel A, Barut B, Korkmaz B, Yayli N. The volatile chemical compositions of the essential Oil/SPME and enzyme inhibitory and radical scavenging activities of solvent extracts and the essential oils from Coronilla orientalis Miller and C. varia L. grows in Turkey. Iran J Pharm Sci. 2019; 18(4): 1831–1842.
  • [22] Colak NU, Yıldırım S, Bozdeveci A, Yaylı N, Çoşkunçelebi K, Fandaklı S, Yaşar A. Essential oil composition, antimicrobial and antioxidant activities of Salvia staminea. Rec Nat Prod. 2018; 12: 86-94.
  • [23] Barry AL. Standards NCCLS. Methods for determining bactericidal activity of antimicrobial agents: approved guideline. National Committee for Clinical Laboratory Standards Wayne, PA, 1999.
  • [24] Woods GL, Brown-Elliott BA, Desmond EP, Hall GS, Heifets LB, Pfyffer GE. Ridderhof JC, Wallace RJJ, Warren NG, Witebsky FG. Susceptibility testing of mycobacteria, nocardiae, and other aerobic actinomycetes; approved standard, NCCLS, 2010.
  • [25] Şimşek Sezer EN, Uysal T. Volatile and phenolic compositions of the leaves of two Vinca L. species from Turkey. Curr Pers MAPs. 2018; 1(2): 103-110.
  • [26] Ashrafi B, Ramak P, Ezatpour B, Talei RG. Biological activity and chemical composition of the essential oil of Nepeta cataria L. J Res Pharm. 2019; 23(2): 336-343.
  • [27] İşcan G, Demirci B, Köse YB. Antimicrobial essential oil of Origanum boissieri Ietswaart. J Res Pharm. 2020; 24(2): 233- 239.
Toplam 27 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Farmakognozi
Bölüm Articles
Yazarlar

İshak Erik

Gözde Kilic

Büşra Korkmaz

Seda Fandakli

Şengül Alpay Karaoğlu

Nurettin Yaylı

Yayımlanma Tarihi 28 Haziran 2025
Yayımlandığı Sayı Yıl 2021 Cilt: 25 Sayı: 5

Kaynak Göster

APA Erik, İ., Kilic, G., Korkmaz, B., Fandakli, S., vd. (2025). Volatile constituents and antimicrobial activity of Vinca major L. subsp. hirsuta (Boiss) stearn grown in Turkey. Journal of Research in Pharmacy, 25(5), 581-588.
AMA Erik İ, Kilic G, Korkmaz B, Fandakli S, Alpay Karaoğlu Ş, Yaylı N. Volatile constituents and antimicrobial activity of Vinca major L. subsp. hirsuta (Boiss) stearn grown in Turkey. J. Res. Pharm. Temmuz 2025;25(5):581-588.
Chicago Erik, İshak, Gözde Kilic, Büşra Korkmaz, Seda Fandakli, Şengül Alpay Karaoğlu, ve Nurettin Yaylı. “Volatile Constituents and Antimicrobial Activity of Vinca Major L. Subsp. Hirsuta (Boiss) Stearn Grown in Turkey”. Journal of Research in Pharmacy 25, sy. 5 (Temmuz 2025): 581-88.
EndNote Erik İ, Kilic G, Korkmaz B, Fandakli S, Alpay Karaoğlu Ş, Yaylı N (01 Temmuz 2025) Volatile constituents and antimicrobial activity of Vinca major L. subsp. hirsuta (Boiss) stearn grown in Turkey. Journal of Research in Pharmacy 25 5 581–588.
IEEE İ. Erik, G. Kilic, B. Korkmaz, S. Fandakli, Ş. Alpay Karaoğlu, ve N. Yaylı, “Volatile constituents and antimicrobial activity of Vinca major L. subsp. hirsuta (Boiss) stearn grown in Turkey”, J. Res. Pharm., c. 25, sy. 5, ss. 581–588, 2025.
ISNAD Erik, İshak vd. “Volatile Constituents and Antimicrobial Activity of Vinca Major L. Subsp. Hirsuta (Boiss) Stearn Grown in Turkey”. Journal of Research in Pharmacy 25/5 (Temmuz 2025), 581-588.
JAMA Erik İ, Kilic G, Korkmaz B, Fandakli S, Alpay Karaoğlu Ş, Yaylı N. Volatile constituents and antimicrobial activity of Vinca major L. subsp. hirsuta (Boiss) stearn grown in Turkey. J. Res. Pharm. 2025;25:581–588.
MLA Erik, İshak vd. “Volatile Constituents and Antimicrobial Activity of Vinca Major L. Subsp. Hirsuta (Boiss) Stearn Grown in Turkey”. Journal of Research in Pharmacy, c. 25, sy. 5, 2025, ss. 581-8.
Vancouver Erik İ, Kilic G, Korkmaz B, Fandakli S, Alpay Karaoğlu Ş, Yaylı N. Volatile constituents and antimicrobial activity of Vinca major L. subsp. hirsuta (Boiss) stearn grown in Turkey. J. Res. Pharm. 2025;25(5):581-8.