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Investigation of the antibacterial, antibiofilm and cytotoxic effects of boron compounds in a Streptococcus mitis infection model on HepG2 liver cell

Yıl 2023, Cilt: 27 Sayı: 6, 2277 - 2284, 28.06.2025

Demet Çelebi , Özgür Çelebi , Sümeyye Başer , Ali Taghizadehghalehjoughi

Öz

Liver abscesses are most commonly attributed to Escherichia coli and Klebsiella pneumoniae, but because Streptococcus mitis is a commensal bacterium with low virulence activity, it is overlooked by its antimicrobial resistance and virulence factors. In this study, we tried to evaluate the synergistic effect of boron compounds and the effect on biofilm in the infection model we created with Streptococcus mitis NCTC 12261 on the HepG2 liver cell line. Minimal inhibitory concentration (MIC) values were determined on Streptococcus mitis at doses of 31.25 µg/ml of Sodium Perborate Metahydrate (SPM), Zinc Borate (ZB) 62.5 µg/ml and Etidote 125 µg/ml. Etidote+SPM, Etidote+ZB, and ZB+SPM combinations showed the most effective fractional inhibitor concentration value synergistic effect at minimum doses of 32 µg/ml+32 µg/ml in all combinations, while it showed an effect on biofilm at doses of 1028 µg/ml+ 1028 µg/ml HepG2 Cell culture.

Anahtar Kelimeler

Biofilm Boron Compounds Liver Cell Synergistic Effect Cytotoxic effect

Kaynakça

  • [1] Marron A, Carratalà J, González-Barca E, Fernández-Sevilla A, Alcaide F, Gudiol F. Serious complications of bacteremia caused by viridans streptococci in neutropenic patients with cancer. Clin Infect Dis. 2000; 31: 1126–1130. https://doi.org/10.1086/317460.
  • [2] Ahmed R, Hassall T, Morland B, Gray J. Viridans streptococcus bacteremia in children on chemotherapy for cancer: an underestimated problem. Pediatr Hematol Oncol. 2003; 20: 439–444. https://doi.org/10.1080/08880010390220144.
  • [3] Hoshino T, Fujiwara T, Kilian M. Use of phylogenetic and phenotypic analyses to identify nonhemolytic streptococci isolated from bacteremic patients. J Clin Microbiol. 2005; 43: 6073–6085. https://doi.org/10.1128/JCM.43.12.6073-6085.2005.
  • [4] Husain E, Whitehead S, Castell A, Thomas EE, Speert DP. Viridans streptococci bacteremia in children with malignancy: relevance of species identification and penicillin susceptibility. Pediatr Infect Dis J. 2005; 24: 563–566. https://doi.org/10.1097/01.inf.0000164708.21464.03.
  • [5] Han XY, Kamana M, Rolston KV. Viridans streptococci isolated by culture from blood of cancer patients: clinical and microbiologic analysis of 50 cases. J Clin Microbiol. 2006; 44: 160–165. https://doi.org/10.1128/JCM.44.1.160-165.2006.
  • [6] Kohno K, Nagafuji K, Tsukamoto H, Horiuchi T, Takase K, Aoki K, Henzan H, Kamezaki K, Takenaka K, Miyamoto T, Teshima T, Harada M, Akashi K. Infectious complications in patients receiving autologous CD34-selected hematopoietic stem cell transplantation for severe autoimmune diseases. Transpl Infect Dis. 2009; 11: 318–323. https://doi.org/10.1111/j.1399-3062.2009.00401.x.
  • [7] Mitchell J. Streptococcus mitis: Walking the line between commensalism and pathogenesis. Mol Oral Microbiol. 2011; 26(2): 89–98. https://doi.org/10.1111/j.2041-1014.2010.0601.x.
  • [8] HPA. Pyogenic and non-pyogenic streptococcal bacteraemias, England, Wales and Northern Ireland: 2008. Health Protection Report [serial online]. 2009; 3(49).
  • [9] Bochud PY, Cometta A, Francioli P. Virulent infections caused by alpha-haemolytic streptococci in cancer patients and their management. Curr Opin Infect Dis. 1997; 10: 422–430. https://doi.org/10.1128/jcm.37.6.1876-1880.1999.
  • [10] Coghi PS, Zhu Y, Xie H, Hosmane NS, Zhang Y. Organoboron Compounds: Effective antibacterial and antiparasitic agents. Molecules. 2021; 26(11): 3309. https://doi.org/10.3390/molecules26113309.
  • [11] Lamas CC, Eykyn SJ. Blood culture negative endocarditis: analysis of 63 cases presenting over 25 years. Heart. 2003; 89: 258–262. https://doi.org/10.1136/heart.89.3.258.
  • [12] Kutlu SS, Sacar S, Cevahir N, Turgut HI. Community-acquired Streptococcus mitis meningitis: a case report. Int J Infect Dis. 2008; 12: 107–109. https://doi.org/10.1016/j.ijid.2008.01.003.
  • [13] Johannsen EC, Sifri CD, Madoff LC. Pyogenic liver abscesses. Infect Dis Clin North Am. 2000; 14: 547–563. https://doi.org/10.1016/s0891-5520(05)70120-3.
  • [14] Duguid R. Copper-inhibition of the growth of oral streptococci and actinomyces. Biomaterials. 1983; 4(3): 225–227. https://doi.org/10.1016/0142-9612(83)90017-0.
  • [15] Seppala H, Haanpera M, Al-Juhaish M, Jarvinen H, Jalava J, Huovinen P. Antimicrobial susceptibility patterns and macrolide resistance genes of viridans group streptococci from normal flora. J Antimicrob Chemother. 2003; 52(4): 636–644. https://doi.org/10.1093/jac/dkg423.
  • [16] Yiş R, Yüksel CN, Derundere U, Yiş U. Meningitis and white matter lesions due to Streptococcus mitis in a previously healthy child. Microbiol Bull. 2011; 45(4): 741–745.
  • [17] https://www.heraproject.com/files/7-f-04-hera%20sodium%20perborate%20full%20web%20wd.pdf. Erişim Tarihi: 16.01.2023.
  • [18] Celebi D, Taghizadehghalehjoughi A, Baser S, Genc S, Yilmaz A, Yeni Y, Yesilyurt F, Yildirim S, Bolat I, Kordali S, Yilmaz F, Hacimuftuoglu A, Celebi O, Margina D, Nitulescu GM, Spandidos DA, Tsatsakis A. Effects of boric acid and potassium metaborate on cytokine levels and redox stress parameters in a wound model infected with methicillin‑resistant Staphylococcus aureus. Mol Med Rep. 2022; 26(3): 294. https://doi.org/10.3892/mmr.2022.12809.

Yıl 2023, Cilt: 27 Sayı: 6, 2277 - 2284, 28.06.2025

Demet Çelebi , Özgür Çelebi , Sümeyye Başer , Ali Taghizadehghalehjoughi

Öz

Kaynakça

  • [1] Marron A, Carratalà J, González-Barca E, Fernández-Sevilla A, Alcaide F, Gudiol F. Serious complications of bacteremia caused by viridans streptococci in neutropenic patients with cancer. Clin Infect Dis. 2000; 31: 1126–1130. https://doi.org/10.1086/317460.
  • [2] Ahmed R, Hassall T, Morland B, Gray J. Viridans streptococcus bacteremia in children on chemotherapy for cancer: an underestimated problem. Pediatr Hematol Oncol. 2003; 20: 439–444. https://doi.org/10.1080/08880010390220144.
  • [3] Hoshino T, Fujiwara T, Kilian M. Use of phylogenetic and phenotypic analyses to identify nonhemolytic streptococci isolated from bacteremic patients. J Clin Microbiol. 2005; 43: 6073–6085. https://doi.org/10.1128/JCM.43.12.6073-6085.2005.
  • [4] Husain E, Whitehead S, Castell A, Thomas EE, Speert DP. Viridans streptococci bacteremia in children with malignancy: relevance of species identification and penicillin susceptibility. Pediatr Infect Dis J. 2005; 24: 563–566. https://doi.org/10.1097/01.inf.0000164708.21464.03.
  • [5] Han XY, Kamana M, Rolston KV. Viridans streptococci isolated by culture from blood of cancer patients: clinical and microbiologic analysis of 50 cases. J Clin Microbiol. 2006; 44: 160–165. https://doi.org/10.1128/JCM.44.1.160-165.2006.
  • [6] Kohno K, Nagafuji K, Tsukamoto H, Horiuchi T, Takase K, Aoki K, Henzan H, Kamezaki K, Takenaka K, Miyamoto T, Teshima T, Harada M, Akashi K. Infectious complications in patients receiving autologous CD34-selected hematopoietic stem cell transplantation for severe autoimmune diseases. Transpl Infect Dis. 2009; 11: 318–323. https://doi.org/10.1111/j.1399-3062.2009.00401.x.
  • [7] Mitchell J. Streptococcus mitis: Walking the line between commensalism and pathogenesis. Mol Oral Microbiol. 2011; 26(2): 89–98. https://doi.org/10.1111/j.2041-1014.2010.0601.x.
  • [8] HPA. Pyogenic and non-pyogenic streptococcal bacteraemias, England, Wales and Northern Ireland: 2008. Health Protection Report [serial online]. 2009; 3(49).
  • [9] Bochud PY, Cometta A, Francioli P. Virulent infections caused by alpha-haemolytic streptococci in cancer patients and their management. Curr Opin Infect Dis. 1997; 10: 422–430. https://doi.org/10.1128/jcm.37.6.1876-1880.1999.
  • [10] Coghi PS, Zhu Y, Xie H, Hosmane NS, Zhang Y. Organoboron Compounds: Effective antibacterial and antiparasitic agents. Molecules. 2021; 26(11): 3309. https://doi.org/10.3390/molecules26113309.
  • [11] Lamas CC, Eykyn SJ. Blood culture negative endocarditis: analysis of 63 cases presenting over 25 years. Heart. 2003; 89: 258–262. https://doi.org/10.1136/heart.89.3.258.
  • [12] Kutlu SS, Sacar S, Cevahir N, Turgut HI. Community-acquired Streptococcus mitis meningitis: a case report. Int J Infect Dis. 2008; 12: 107–109. https://doi.org/10.1016/j.ijid.2008.01.003.
  • [13] Johannsen EC, Sifri CD, Madoff LC. Pyogenic liver abscesses. Infect Dis Clin North Am. 2000; 14: 547–563. https://doi.org/10.1016/s0891-5520(05)70120-3.
  • [14] Duguid R. Copper-inhibition of the growth of oral streptococci and actinomyces. Biomaterials. 1983; 4(3): 225–227. https://doi.org/10.1016/0142-9612(83)90017-0.
  • [15] Seppala H, Haanpera M, Al-Juhaish M, Jarvinen H, Jalava J, Huovinen P. Antimicrobial susceptibility patterns and macrolide resistance genes of viridans group streptococci from normal flora. J Antimicrob Chemother. 2003; 52(4): 636–644. https://doi.org/10.1093/jac/dkg423.
  • [16] Yiş R, Yüksel CN, Derundere U, Yiş U. Meningitis and white matter lesions due to Streptococcus mitis in a previously healthy child. Microbiol Bull. 2011; 45(4): 741–745.
  • [17] https://www.heraproject.com/files/7-f-04-hera%20sodium%20perborate%20full%20web%20wd.pdf. Erişim Tarihi: 16.01.2023.
  • [18] Celebi D, Taghizadehghalehjoughi A, Baser S, Genc S, Yilmaz A, Yeni Y, Yesilyurt F, Yildirim S, Bolat I, Kordali S, Yilmaz F, Hacimuftuoglu A, Celebi O, Margina D, Nitulescu GM, Spandidos DA, Tsatsakis A. Effects of boric acid and potassium metaborate on cytokine levels and redox stress parameters in a wound model infected with methicillin‑resistant Staphylococcus aureus. Mol Med Rep. 2022; 26(3): 294. https://doi.org/10.3892/mmr.2022.12809.
Toplam 18 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Eczacılık ve İlaç Bilimleri (Diğer)
Bölüm Articles
Yazarlar

Demet Çelebi 0000-0002-2355-0561

Özgür Çelebi 0000-0003-4578-9474

Sümeyye Başer

Ali Taghizadehghalehjoughi 0000-0002-3506-0324

Yayımlanma Tarihi 28 Haziran 2025
Yayımlandığı Sayı Yıl 2023 Cilt: 27 Sayı: 6

Kaynak Göster

APA Çelebi, D., Çelebi, Ö., Başer, S., Taghizadehghalehjoughi, A. (2025). Investigation of the antibacterial, antibiofilm and cytotoxic effects of boron compounds in a Streptococcus mitis infection model on HepG2 liver cell. Journal of Research in Pharmacy, 27(6), 2277-2284. https://doi.org/10.29228/jrp.516
AMA Çelebi D, Çelebi Ö, Başer S, Taghizadehghalehjoughi A. Investigation of the antibacterial, antibiofilm and cytotoxic effects of boron compounds in a Streptococcus mitis infection model on HepG2 liver cell. J. Res. Pharm. Temmuz 2025;27(6):2277-2284. doi:10.29228/jrp.516
Chicago Çelebi, Demet, Özgür Çelebi, Sümeyye Başer, ve Ali Taghizadehghalehjoughi. “Investigation of the Antibacterial, Antibiofilm and Cytotoxic Effects of Boron Compounds in a Streptococcus Mitis Infection Model on HepG2 Liver Cell”. Journal of Research in Pharmacy 27, sy. 6 (Temmuz 2025): 2277-84. https://doi.org/10.29228/jrp.516.
EndNote Çelebi D, Çelebi Ö, Başer S, Taghizadehghalehjoughi A (01 Temmuz 2025) Investigation of the antibacterial, antibiofilm and cytotoxic effects of boron compounds in a Streptococcus mitis infection model on HepG2 liver cell. Journal of Research in Pharmacy 27 6 2277–2284.
IEEE D. Çelebi, Ö. Çelebi, S. Başer, ve A. Taghizadehghalehjoughi, “Investigation of the antibacterial, antibiofilm and cytotoxic effects of boron compounds in a Streptococcus mitis infection model on HepG2 liver cell”, J. Res. Pharm., c. 27, sy. 6, ss. 2277–2284, 2025, doi: 10.29228/jrp.516.
ISNAD Çelebi, Demet vd. “Investigation of the Antibacterial, Antibiofilm and Cytotoxic Effects of Boron Compounds in a Streptococcus Mitis Infection Model on HepG2 Liver Cell”. Journal of Research in Pharmacy 27/6 (Temmuz 2025), 2277-2284. https://doi.org/10.29228/jrp.516.
JAMA Çelebi D, Çelebi Ö, Başer S, Taghizadehghalehjoughi A. Investigation of the antibacterial, antibiofilm and cytotoxic effects of boron compounds in a Streptococcus mitis infection model on HepG2 liver cell. J. Res. Pharm. 2025;27:2277–2284.
MLA Çelebi, Demet vd. “Investigation of the Antibacterial, Antibiofilm and Cytotoxic Effects of Boron Compounds in a Streptococcus Mitis Infection Model on HepG2 Liver Cell”. Journal of Research in Pharmacy, c. 27, sy. 6, 2025, ss. 2277-84, doi:10.29228/jrp.516.
Vancouver Çelebi D, Çelebi Ö, Başer S, Taghizadehghalehjoughi A. Investigation of the antibacterial, antibiofilm and cytotoxic effects of boron compounds in a Streptococcus mitis infection model on HepG2 liver cell. J. Res. Pharm. 2025;27(6):2277-84.