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Antioxidant evaluation and HPLC analysis of Buchanania lanzan and Buchanania siamensis leaf extracts

Yıl 2023, Cilt: 27 Sayı: 6, 2480 - 2486, 28.06.2025

Chadaporn Prompanya Arpa Petchsomrit Boonyadist Vongsak

Öz

Buchanania lanzan Spreng and Buchanania siamensis Miq. (Anacardiaceae) are traditional medicinal plants that are rich in phenolic compounds and are used for the treatment of inflammatory diseases and pain. In the present study, the antioxidant potential of the distilled water and 80% ethanol extracts of B. lanzan and B. siamensis leaves was evaluated by various antioxidant assays, including DPPH, ABTS, FRAP, and ROS assays. The simultaneous HPLC determination of three major compounds was developed and validated. In addition, the total phenolic contents of each extract were also measured. From HPLC parameter validation, good linearity was achieved, with a correlation coefficient greater than 0.997. The method provided acceptable accuracy, specificity, and precision, suggesting that it could be suitably used for the quantification of the major compounds (myricitrin, isoquercitrin, and quercitrin) in the crude extracts of B. lanzan and B. siamensis. The results of the antioxidant assays showed that the ethanol extracts exhibited higher antioxidant activity than the aqueous extracts. The ethanol extract of B. lanzan leaves expressed the most antioxidant activity and could serve as a potential antioxidant source. Moreover, our validated technique would be beneficial for the quality control of the extract.

Anahtar Kelimeler

Antioxidant Buchanania HPLC ROS validated

Kaynakça

  • [1] Vongsak B, Kongkiatpaiboon S, Jaisamut S, Konsap K. Comparison of active constituents, antioxidant capacity, and α-glucosidase inhibition in Pluchea indica leaf extracts at different maturity stages. Food Biosci. 2018; 25: 68-73. https://doi.org/10.1016/j.fbio.2018.08.006
  • [2] Vongsak B, Jaisamut S, Gonsap K. Optimization of Maclura cochinchinensis extract as a cosmeceutical component for antioxidant and anti-tyrosinase activities. Key Eng Mater. 2020; 859: 188-193. https://doi.org/10.4028/www.scientific.net/KEM.859.188
  • [3] Rajput BS, Gupta D, Kumar S, Singh K, Tiwari C. Buchanania lanzan Spreng (Chironji): A vulnerable multipurpose tree species in Vindhyan region. J Pharmacogn Phytochem. 2018; 7(5): 833-836.
  • [4] Jain R, Jain SK. Effect of Buchanania lanzan Spreng. bark extract on cyclophosphamide induced genotoxicity and oxidative stress in mice. Asian Pac J Trop Med. 2012; 5(3): 187-191. https://doi.org/10.1016/S1995-7645(12)60022-4
  • [5] Yodsaoue O, Kamonwannasit S, Fangkrathok N. Antioxidant and anticancer activities of Buchanania siamensis Miq. stem and leaf extracts. J Sci Tech UBU. 2017; Special issue: 13-17.
  • [6] Schulze-Kaysers N, Feuereisen MM, Schieber A. Phenolic compounds in edible species of the Anacardiaceae family: A review. RSC Adv. 2015; 5(89): 73301-73314. https://doi.org/10.1039/C5RA11746A
  • [7] Umadevi I, Daniel M, Sabnis SD. Chemotaxonomic studies on some members of Anacardiaceae. Proc Indian Acad Sci. 1988; 98: 205-208. https://doi.org/10.1007/BF03053589
  • [8] Domitrović R, Rashed K, Cvijanović O, Vladimir-Knežević S, Škoda M, Višnić A. Myricitrin exhibits antioxidant, anti-inflammatory and antifibrotic activity in carbon tetrachloride-intoxicated mice. Chem Biol Interact. 2015; 230: 21-29. https://doi.org/10.1016/j.cbi.2015.01.030
  • [9] Comalada M, Camuesco D, Sierra S, Balleser I, Xaus J, Galves J, Zarzuelo A. In vivo quercitrin anti-inflammatory effect involves release of quercetin, which inhibits inflammation through down-regulation of the NF-κB pathway. Eur J Immunol. 2005; 35(2): 584-592. https://doi.org/10.1002/eji.200425778
  • [10] Li X, Jiang Q, Wang T, Liu J, Chen D. Comparison of the antioxidant effects of quercitrin and isoquercitrin: understanding the role of the 6’’-OH group. Molecules. 2016; 21(9): 1246. https://doi.org/10.3390/molecules21091246
  • [11] Aryal S, Baniya MK, Danekhu K, Kunwar P, Gurung R, Koirala N. Total phenolic content, flavonoid content and antioxidant potential of wild vegetables from western Nepal. Plants. 2019; 8(4): 96. https://doi.org/10.3390/plants8040096
  • [12] Safdar MN, Kausar T, Nadeem M. Comparison of ultrasound and maceration techniques for the extraction of polyphenols from the mango peel. J Food Process Preserv. 2016; 41(4): e13028. https://doi.org/10.1111/jfpp.13028
  • [13] Oroian M, Dranca F, Ursachi F. Comparative evaluation of maceration, microwave and ultrasonic-assisted extraction of phenolic compounds from propolis. J Food Sci Technol. 2020; 57(1): 70-78. https://doi.org/10.1007/s13197-019-04031-x
  • [14] Petchsomrit A, Chanthathamrongsiri N, Manmuan S, Leelakanok N, Wangpradit N, Vongsak B, Sirirak T. Green extraction of Buchanania siamensis and water-based formulation. Sustain Chem Pharm. 2022; 30: 100883. https://doi.org/10.1016/j.scp.2022.100883
  • [15] Shabir GA. Validation of high-performance liquid chromatography methods for pharmaceutical analysis: understanding the differences and similarities between validation requirements of the US Food and Drug Administration, the US Pharmacopeia and the International Conference on harmonization. J Chromatogr A. 2003; 987(1-2): 57-66. https://doi.org/10.1016/S0021-9673(02)01536-4
  • [16] Pattananandecha T, Apichai S, Julsrigival J, Ungsurungsie M, Samuhasaneetoo S, Chulasiri P, Kwankhao P, Pitiporn S, Ogata F, Kawasaki N, Saenjum C. Antioxidant activity and anti-photoaging effects on UVA-irradiated human fibroblasts of rosmarinic acid enriched extract prepared from Thunbergia laurifolia leaves. Plants. 2021; 10(8): 1648. https://doi.org/10.3390/plants10081648
  • [17] International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use. (2005). Validation of analytical procedures: text and methodology Q2(R1) [ICH Harmonised Tripartite Guideline]. https://database.ich.org/sites/default/files/Q2%28R1%29%20Guideline.pdf

Yıl 2023, Cilt: 27 Sayı: 6, 2480 - 2486, 28.06.2025

Chadaporn Prompanya Arpa Petchsomrit Boonyadist Vongsak

Öz

Kaynakça

  • [1] Vongsak B, Kongkiatpaiboon S, Jaisamut S, Konsap K. Comparison of active constituents, antioxidant capacity, and α-glucosidase inhibition in Pluchea indica leaf extracts at different maturity stages. Food Biosci. 2018; 25: 68-73. https://doi.org/10.1016/j.fbio.2018.08.006
  • [2] Vongsak B, Jaisamut S, Gonsap K. Optimization of Maclura cochinchinensis extract as a cosmeceutical component for antioxidant and anti-tyrosinase activities. Key Eng Mater. 2020; 859: 188-193. https://doi.org/10.4028/www.scientific.net/KEM.859.188
  • [3] Rajput BS, Gupta D, Kumar S, Singh K, Tiwari C. Buchanania lanzan Spreng (Chironji): A vulnerable multipurpose tree species in Vindhyan region. J Pharmacogn Phytochem. 2018; 7(5): 833-836.
  • [4] Jain R, Jain SK. Effect of Buchanania lanzan Spreng. bark extract on cyclophosphamide induced genotoxicity and oxidative stress in mice. Asian Pac J Trop Med. 2012; 5(3): 187-191. https://doi.org/10.1016/S1995-7645(12)60022-4
  • [5] Yodsaoue O, Kamonwannasit S, Fangkrathok N. Antioxidant and anticancer activities of Buchanania siamensis Miq. stem and leaf extracts. J Sci Tech UBU. 2017; Special issue: 13-17.
  • [6] Schulze-Kaysers N, Feuereisen MM, Schieber A. Phenolic compounds in edible species of the Anacardiaceae family: A review. RSC Adv. 2015; 5(89): 73301-73314. https://doi.org/10.1039/C5RA11746A
  • [7] Umadevi I, Daniel M, Sabnis SD. Chemotaxonomic studies on some members of Anacardiaceae. Proc Indian Acad Sci. 1988; 98: 205-208. https://doi.org/10.1007/BF03053589
  • [8] Domitrović R, Rashed K, Cvijanović O, Vladimir-Knežević S, Škoda M, Višnić A. Myricitrin exhibits antioxidant, anti-inflammatory and antifibrotic activity in carbon tetrachloride-intoxicated mice. Chem Biol Interact. 2015; 230: 21-29. https://doi.org/10.1016/j.cbi.2015.01.030
  • [9] Comalada M, Camuesco D, Sierra S, Balleser I, Xaus J, Galves J, Zarzuelo A. In vivo quercitrin anti-inflammatory effect involves release of quercetin, which inhibits inflammation through down-regulation of the NF-κB pathway. Eur J Immunol. 2005; 35(2): 584-592. https://doi.org/10.1002/eji.200425778
  • [10] Li X, Jiang Q, Wang T, Liu J, Chen D. Comparison of the antioxidant effects of quercitrin and isoquercitrin: understanding the role of the 6’’-OH group. Molecules. 2016; 21(9): 1246. https://doi.org/10.3390/molecules21091246
  • [11] Aryal S, Baniya MK, Danekhu K, Kunwar P, Gurung R, Koirala N. Total phenolic content, flavonoid content and antioxidant potential of wild vegetables from western Nepal. Plants. 2019; 8(4): 96. https://doi.org/10.3390/plants8040096
  • [12] Safdar MN, Kausar T, Nadeem M. Comparison of ultrasound and maceration techniques for the extraction of polyphenols from the mango peel. J Food Process Preserv. 2016; 41(4): e13028. https://doi.org/10.1111/jfpp.13028
  • [13] Oroian M, Dranca F, Ursachi F. Comparative evaluation of maceration, microwave and ultrasonic-assisted extraction of phenolic compounds from propolis. J Food Sci Technol. 2020; 57(1): 70-78. https://doi.org/10.1007/s13197-019-04031-x
  • [14] Petchsomrit A, Chanthathamrongsiri N, Manmuan S, Leelakanok N, Wangpradit N, Vongsak B, Sirirak T. Green extraction of Buchanania siamensis and water-based formulation. Sustain Chem Pharm. 2022; 30: 100883. https://doi.org/10.1016/j.scp.2022.100883
  • [15] Shabir GA. Validation of high-performance liquid chromatography methods for pharmaceutical analysis: understanding the differences and similarities between validation requirements of the US Food and Drug Administration, the US Pharmacopeia and the International Conference on harmonization. J Chromatogr A. 2003; 987(1-2): 57-66. https://doi.org/10.1016/S0021-9673(02)01536-4
  • [16] Pattananandecha T, Apichai S, Julsrigival J, Ungsurungsie M, Samuhasaneetoo S, Chulasiri P, Kwankhao P, Pitiporn S, Ogata F, Kawasaki N, Saenjum C. Antioxidant activity and anti-photoaging effects on UVA-irradiated human fibroblasts of rosmarinic acid enriched extract prepared from Thunbergia laurifolia leaves. Plants. 2021; 10(8): 1648. https://doi.org/10.3390/plants10081648
  • [17] International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use. (2005). Validation of analytical procedures: text and methodology Q2(R1) [ICH Harmonised Tripartite Guideline]. https://database.ich.org/sites/default/files/Q2%28R1%29%20Guideline.pdf
Toplam 17 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Eczacılık ve İlaç Bilimleri (Diğer)
Bölüm Articles
Yazarlar

Chadaporn Prompanya 0000-0001-9102-9674

Arpa Petchsomrit 0000-0003-3863-9451

Boonyadist Vongsak 0000-0002-2284-1439

Yayımlanma Tarihi 28 Haziran 2025
Yayımlandığı Sayı Yıl 2023 Cilt: 27 Sayı: 6

Kaynak Göster

APA Prompanya, C., Petchsomrit, A., & Vongsak, B. (2025). Antioxidant evaluation and HPLC analysis of Buchanania lanzan and Buchanania siamensis leaf extracts. Journal of Research in Pharmacy, 27(6), 2480-2486.
AMA Prompanya C, Petchsomrit A, Vongsak B. Antioxidant evaluation and HPLC analysis of Buchanania lanzan and Buchanania siamensis leaf extracts. J. Res. Pharm. Temmuz 2025;27(6):2480-2486.
Chicago Prompanya, Chadaporn, Arpa Petchsomrit, ve Boonyadist Vongsak. “Antioxidant Evaluation and HPLC Analysis of Buchanania Lanzan and Buchanania Siamensis Leaf Extracts”. Journal of Research in Pharmacy 27, sy. 6 (Temmuz 2025): 2480-86.
EndNote Prompanya C, Petchsomrit A, Vongsak B (01 Temmuz 2025) Antioxidant evaluation and HPLC analysis of Buchanania lanzan and Buchanania siamensis leaf extracts. Journal of Research in Pharmacy 27 6 2480–2486.
IEEE C. Prompanya, A. Petchsomrit, ve B. Vongsak, “Antioxidant evaluation and HPLC analysis of Buchanania lanzan and Buchanania siamensis leaf extracts”, J. Res. Pharm., c. 27, sy. 6, ss. 2480–2486, 2025.
ISNAD Prompanya, Chadaporn vd. “Antioxidant Evaluation and HPLC Analysis of Buchanania Lanzan and Buchanania Siamensis Leaf Extracts”. Journal of Research in Pharmacy 27/6 (Temmuz 2025), 2480-2486.
JAMA Prompanya C, Petchsomrit A, Vongsak B. Antioxidant evaluation and HPLC analysis of Buchanania lanzan and Buchanania siamensis leaf extracts. J. Res. Pharm. 2025;27:2480–2486.
MLA Prompanya, Chadaporn vd. “Antioxidant Evaluation and HPLC Analysis of Buchanania Lanzan and Buchanania Siamensis Leaf Extracts”. Journal of Research in Pharmacy, c. 27, sy. 6, 2025, ss. 2480-6.
Vancouver Prompanya C, Petchsomrit A, Vongsak B. Antioxidant evaluation and HPLC analysis of Buchanania lanzan and Buchanania siamensis leaf extracts. J. Res. Pharm. 2025;27(6):2480-6.