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Therapeutic effect of Anethum graveolens L. (Dill) seed extract for the treatment of dextran sodium sulfate (DSS) induced ulcerative colitis in Wistar rats

Yıl 2024, Cilt: 28 Sayı: 3, 770 - 780, 28.06.2025

Karthick Kannan Rajendran Indumathy Indumathy

Öz

The purpose of this study was to determine whether the Anethum graveolens L. seed (AGS) extract has any anti-oxidant, anti-inflammatory, or immune-modulatory activities against Ulcerative colitis (UC). AGS extract has found widespread use in folk medicine. Twenty seven (n=27) adult female Wistar albino rats were employed for the experiment involving Dextran Sodium Sulfate (DSS) induced UC. These rats were distributed randomly into five groups, each consisting of six animals (n=6), with the exception of group 1, which had three animals (n=3). Group 1 received 0.5% Carboxy methyl cellulose (CMC) (p.o.), Group 2 received 5% DSS (p.o.), Group 3 received 350 mg/kg b.w. mesalazine (p.o.), and Groups 4, 5, respectively, received 200 and 400 mg/kg AGS extract (p.o). Along with administering DSS, the treatment lasted for 12 days. In comparison to the DSS-treated group, groups 4, and 5, there was a considerable weight gain. Comparing groups 4 and 5 to group 2, the groups 4 and 5 showed a substantial decrease in Disease Activity Index (DAI), colon damage (p<0.05), spleen weight (p<0.05), serum IL-6 (p<0.0001), TNF-α (p<0.01), and IL-17 (p<0.01) levels. Rats in groups 4 and 5 had significantly higher blood IL-10 levels (p<0.05) and longer colons than rats in group 2 (p<0.05). The good results, which showed an improvement in the state of the colon epithelium after AGS extract in treatment groups, were validated by a histopathological study. In summary, AGS extract may be able to control inflammatory reactions and safeguard the colon epithelium in UC. These results support the possible therapeutic application of AGS extract.

Anahtar Kelimeler

Anethum graveolens L. ulcerative colitis dextran sodium sulfate antioxidant anti-inflammatory

Kaynakça

  • [1] Lin S, Lau LH, Chanchlani N, Kennedy NA, Ng SC. Recent advances in clinical practice: management of inflammatory bowel disease during the COVID-19 pandemic. BMJ. 2022; 71(7): 1426-1439. http://doi.org/10.1136/gutjnl-2021-326784
  • [2] Actis GC, Pellicano R, Fagoonee S, Ribaldone DG. History of Inflammatory Bowel Diseases. J Clin Med. 2019; 8(11): 1970. http://dx.doi.org/10.3390/jcm8111970
  • [3] Wang R, Luo Y, Lu Y, Wang D, Wang T, Pu W, Wang Y. Maggot Extracts Alleviate Inflammation and Oxidative Stress in Acute Experimental Colitis via the Activation of Nrf2. Oxid Med Cell Longev. 2019;2019:4703253. https://doi.org/10.1155/2019/4703253
  • [4] Tripathi K, Feuerstein JD. New developments in ulcerative colitis: latest evidence on management, treatment, and maintenance. Drugs Context. 2019;8:212572. https://doi.org/10.7573/dic.212572
  • [5] Gerriets V, Goyal A, Khaddour K. Tumor Necrosis Factor Inhibitors. StatPearls [Internet], Treasure Island (FL), 2022.
  • [6] Safarini O, Keshavamurthy C et al. Calcineurin inhibitors. StatPearls [Internet], Treasure Island (FL), 2022.
  • [7] Park SC, Jeen YT. Anti-integrin therapy for inflammatory bowel disease. World J Gastroenterol. 2018; 24(17): 1868- 1880. https://doi.org/10.3748/wjg.v24.i17.1868
  • [8] Triantafyllidi A, Xanthos T, Papalois A, Triantafillidis JK. Herbal and plant therapy in patients with inflammatory bowel disease. Ann Gastroenterol. 2015; 28(2): 210-220.
  • [9] Wan P, Chen H, Guo Y, Bai AP. Advances in treatment of ulcerative colitis with herbs: from bench to bedside. World J Gastroenterol. 2014; 20(39): 14099-14104. https://doi.org/10.3748/wjg.v20.i39.14099
  • [10] Naseri M, Mojab F, Khodadoost M, Kamalinejad M, Davati A, Choopani R, Hasheminejad A, Bararpoor Z, Shariatpanahi S, Emtiazy M. The study of anti-inflammatory activity of oil-based dill (Anethum graveolens L.) extract used topically in formalin-induced maggot extracts alleviate inflammation and oxidative stress in acute experimental colitis via the activation of Nrf2Inflammation male rat paw. Iran J Pharm Res. 2012; 11(4): 1169-1174.
  • [11] Jana S, Shekhawat GS. Anethum graveolens: An Indian traditional medicinal herb and spice. Pharmacogn Rev. 2010; 4(8): 179-184. https://doi.org/10.4103/0973-7847.70915
  • [12] Al-Oqail MM, Farshori NN. Antioxidant and anticancer efficacies of Anethum graveolens against human breast carcinoma cells through oxidative stress and caspase dependency. Biomed Res Int. 2021;2021:5535570. https://doi.org/10.1155/2021/5535570
  • [13] Tamilarasi R, Sivanesan D, Kanimozhi P. Hepatoprotective and antioxidant efficacy of Anethum graveolens linn in carbon tetrachloride induced hepatotoxicity in albino rats. J Chem Pharm Res. 2012; 4(4): 1885-1888.
  • [14] Nam HH, Nan L, Choo BK. Anti-Inflammation and protective effects of Anethum graveolens L. (dill seeds) on esophageal mucosa damages in reflux esophagitis-ınduced rats. Foods. 2021; 10(10): 2500. https://doi.org/10.3390/foods10102500
  • [15] Rezaee-Asl M, Bakhtiarian A, Nikoui V, Sabour M, Ostadhadi S, Yadavar-Nikravesh MS, Giorgi M. Antinociceptive properties of hydroalcoholic extracts of Anethum graveolens L. (dill) seed and aerial parts in mice. Clin Exp Pharmacol. 2013; 3:2. https://doi.org/10.4172/2161-1459.1000122
  • [16] Dahiya P, Purkayastha S. Phytochemical analysis and antibacterial efficacy of dill seed oil against multi-drug resistant clinical isolates. Asian J Pharm Clin Res. 2012; 5(2): 62-64.
  • [17] Al-Sheddi ES, Al-Zaid NA, Al-Oqail MM, Al-Massarani SM, El-Gamal AA, Farshori NN. Evaluation of cytotoxicity, cell cycle arrest and apoptosis induced by Anethum graveolens L. essential oil in human hepatocellular carcinoma cell line. Saudi Pharm J. 2019; 27(7): 1053-1060. https://doi.org/10.1016/j.jsps.2019.09.001
  • [18] Anandakumar P, Kamaraj S, Vanitha MK. D-limonene: A multifunctional compound with potent therapeutic effects. J Food Biochem. 2021;45(1):e13566. https://doi.org/10.1111/jfbc.13566
  • [19] Bouyahya A, Mechchate H, Benali T, Ghchime R, Charfi S, Balahbib A, Burkov P, Shariati MA, Lorenzo JM, Omari NE. Health benefits and pharmacological properties of carvone. Biomolecules. 2021; 11(12): 1803. https://doi.org/10.3390/biom11121803
  • [20] Saleh-E-In MM, Sultana N, Rahim MM, Ahsan MA, Bhuiyan MN, Hossain MN, Rahman MM, Kumar Roy S, Islam MR. Chemical composition and pharmacological significance of Anethum sowa L. root. BMC Complementary Altern Med 2017; 17(1): 127. https://doi.org/10.1186/s12906-016-1438-9
  • [21] Wang R, Wang D, Wang H, Wang T, Weng Y, Zhang Y, Luo Y, Lu Y, Wang Y. Therapeutic targeting of Nrf2 signaling by maggot extracts ameliorates ınflammation-associated ıntestinal fibrosis in chronic DSS-Induced colitis. Front Immunol. 2021;12:670159.https://doi.org/10.3389/fimmu.2021.670159
  • [22] Chen Z, Ni W, Yang C, Zhang T, Lu S, Zhao R, Mao X, Yu J. Therapeutic Effect of Amomum villosum on inflammatory bowel disease in rats. Front Pharmacol. 2018; 9: 639. https://doi.org/10.3389/fphar.2018.00639
  • [23] Chassaing B, Aitken JD, Malleshappa M, Vijay-Kumar M. Dextran sulfate sodium (DSS)-induced colitis in mice. Curr Protoc Immunol. 2014;104:15.25.1-15.25.14. https://doi.org/10.1002/0471142735.im1525s104.
  • [24] Chen L, Zhou Z, Yang Y, Chen N, Xiang H. Therapeutic effect of imiquimod on dextran sulfate sodium-induced ulcerative colitis in mice. PLoS One. 2017;12(10):e0186138. https://doi.org/10.1371/journal.pone.0186138
  • [25] Prakash T, Janadri S. Anti-inflammatory effect of wedelolactone on DSS induced colitis in rats: IL-6/STAT3 signaling pathway. J Ayurveda Integr Med. 2023;14(2):100544. https://doi.org/10.1016/j.jaim.2022.100544
  • [26] Yang N, Liang G, Lin J, Zhang S, Lin Q, Ji X, Chen H, Li N, Jin S. Ginsenoside Rd therapy improves histological and functional recovery in a rat model of inflammatory bowel disease. Phytother Res. 2020; 34(11): 3019-3028. https://doi.org/10.1002/ptr.6734
  • [27] Bidgoli SA, Taheri E, Ziarati P. Safety evaluation of oral Anethum graveolens L total hydroalcoholic extract in mice. Asian J Pharm Health Sci. 2012; 1(2): 61-67.
  • [28] Acute Oral Toxicity – Acute Toxic Class Method. OECD Guideline for Testing of Chemicals No. 423. www.oecd.org/env/testguidelines (accessed 2022 Oct 18).

Yıl 2024, Cilt: 28 Sayı: 3, 770 - 780, 28.06.2025

Karthick Kannan Rajendran Indumathy Indumathy

Öz

Kaynakça

  • [1] Lin S, Lau LH, Chanchlani N, Kennedy NA, Ng SC. Recent advances in clinical practice: management of inflammatory bowel disease during the COVID-19 pandemic. BMJ. 2022; 71(7): 1426-1439. http://doi.org/10.1136/gutjnl-2021-326784
  • [2] Actis GC, Pellicano R, Fagoonee S, Ribaldone DG. History of Inflammatory Bowel Diseases. J Clin Med. 2019; 8(11): 1970. http://dx.doi.org/10.3390/jcm8111970
  • [3] Wang R, Luo Y, Lu Y, Wang D, Wang T, Pu W, Wang Y. Maggot Extracts Alleviate Inflammation and Oxidative Stress in Acute Experimental Colitis via the Activation of Nrf2. Oxid Med Cell Longev. 2019;2019:4703253. https://doi.org/10.1155/2019/4703253
  • [4] Tripathi K, Feuerstein JD. New developments in ulcerative colitis: latest evidence on management, treatment, and maintenance. Drugs Context. 2019;8:212572. https://doi.org/10.7573/dic.212572
  • [5] Gerriets V, Goyal A, Khaddour K. Tumor Necrosis Factor Inhibitors. StatPearls [Internet], Treasure Island (FL), 2022.
  • [6] Safarini O, Keshavamurthy C et al. Calcineurin inhibitors. StatPearls [Internet], Treasure Island (FL), 2022.
  • [7] Park SC, Jeen YT. Anti-integrin therapy for inflammatory bowel disease. World J Gastroenterol. 2018; 24(17): 1868- 1880. https://doi.org/10.3748/wjg.v24.i17.1868
  • [8] Triantafyllidi A, Xanthos T, Papalois A, Triantafillidis JK. Herbal and plant therapy in patients with inflammatory bowel disease. Ann Gastroenterol. 2015; 28(2): 210-220.
  • [9] Wan P, Chen H, Guo Y, Bai AP. Advances in treatment of ulcerative colitis with herbs: from bench to bedside. World J Gastroenterol. 2014; 20(39): 14099-14104. https://doi.org/10.3748/wjg.v20.i39.14099
  • [10] Naseri M, Mojab F, Khodadoost M, Kamalinejad M, Davati A, Choopani R, Hasheminejad A, Bararpoor Z, Shariatpanahi S, Emtiazy M. The study of anti-inflammatory activity of oil-based dill (Anethum graveolens L.) extract used topically in formalin-induced maggot extracts alleviate inflammation and oxidative stress in acute experimental colitis via the activation of Nrf2Inflammation male rat paw. Iran J Pharm Res. 2012; 11(4): 1169-1174.
  • [11] Jana S, Shekhawat GS. Anethum graveolens: An Indian traditional medicinal herb and spice. Pharmacogn Rev. 2010; 4(8): 179-184. https://doi.org/10.4103/0973-7847.70915
  • [12] Al-Oqail MM, Farshori NN. Antioxidant and anticancer efficacies of Anethum graveolens against human breast carcinoma cells through oxidative stress and caspase dependency. Biomed Res Int. 2021;2021:5535570. https://doi.org/10.1155/2021/5535570
  • [13] Tamilarasi R, Sivanesan D, Kanimozhi P. Hepatoprotective and antioxidant efficacy of Anethum graveolens linn in carbon tetrachloride induced hepatotoxicity in albino rats. J Chem Pharm Res. 2012; 4(4): 1885-1888.
  • [14] Nam HH, Nan L, Choo BK. Anti-Inflammation and protective effects of Anethum graveolens L. (dill seeds) on esophageal mucosa damages in reflux esophagitis-ınduced rats. Foods. 2021; 10(10): 2500. https://doi.org/10.3390/foods10102500
  • [15] Rezaee-Asl M, Bakhtiarian A, Nikoui V, Sabour M, Ostadhadi S, Yadavar-Nikravesh MS, Giorgi M. Antinociceptive properties of hydroalcoholic extracts of Anethum graveolens L. (dill) seed and aerial parts in mice. Clin Exp Pharmacol. 2013; 3:2. https://doi.org/10.4172/2161-1459.1000122
  • [16] Dahiya P, Purkayastha S. Phytochemical analysis and antibacterial efficacy of dill seed oil against multi-drug resistant clinical isolates. Asian J Pharm Clin Res. 2012; 5(2): 62-64.
  • [17] Al-Sheddi ES, Al-Zaid NA, Al-Oqail MM, Al-Massarani SM, El-Gamal AA, Farshori NN. Evaluation of cytotoxicity, cell cycle arrest and apoptosis induced by Anethum graveolens L. essential oil in human hepatocellular carcinoma cell line. Saudi Pharm J. 2019; 27(7): 1053-1060. https://doi.org/10.1016/j.jsps.2019.09.001
  • [18] Anandakumar P, Kamaraj S, Vanitha MK. D-limonene: A multifunctional compound with potent therapeutic effects. J Food Biochem. 2021;45(1):e13566. https://doi.org/10.1111/jfbc.13566
  • [19] Bouyahya A, Mechchate H, Benali T, Ghchime R, Charfi S, Balahbib A, Burkov P, Shariati MA, Lorenzo JM, Omari NE. Health benefits and pharmacological properties of carvone. Biomolecules. 2021; 11(12): 1803. https://doi.org/10.3390/biom11121803
  • [20] Saleh-E-In MM, Sultana N, Rahim MM, Ahsan MA, Bhuiyan MN, Hossain MN, Rahman MM, Kumar Roy S, Islam MR. Chemical composition and pharmacological significance of Anethum sowa L. root. BMC Complementary Altern Med 2017; 17(1): 127. https://doi.org/10.1186/s12906-016-1438-9
  • [21] Wang R, Wang D, Wang H, Wang T, Weng Y, Zhang Y, Luo Y, Lu Y, Wang Y. Therapeutic targeting of Nrf2 signaling by maggot extracts ameliorates ınflammation-associated ıntestinal fibrosis in chronic DSS-Induced colitis. Front Immunol. 2021;12:670159.https://doi.org/10.3389/fimmu.2021.670159
  • [22] Chen Z, Ni W, Yang C, Zhang T, Lu S, Zhao R, Mao X, Yu J. Therapeutic Effect of Amomum villosum on inflammatory bowel disease in rats. Front Pharmacol. 2018; 9: 639. https://doi.org/10.3389/fphar.2018.00639
  • [23] Chassaing B, Aitken JD, Malleshappa M, Vijay-Kumar M. Dextran sulfate sodium (DSS)-induced colitis in mice. Curr Protoc Immunol. 2014;104:15.25.1-15.25.14. https://doi.org/10.1002/0471142735.im1525s104.
  • [24] Chen L, Zhou Z, Yang Y, Chen N, Xiang H. Therapeutic effect of imiquimod on dextran sulfate sodium-induced ulcerative colitis in mice. PLoS One. 2017;12(10):e0186138. https://doi.org/10.1371/journal.pone.0186138
  • [25] Prakash T, Janadri S. Anti-inflammatory effect of wedelolactone on DSS induced colitis in rats: IL-6/STAT3 signaling pathway. J Ayurveda Integr Med. 2023;14(2):100544. https://doi.org/10.1016/j.jaim.2022.100544
  • [26] Yang N, Liang G, Lin J, Zhang S, Lin Q, Ji X, Chen H, Li N, Jin S. Ginsenoside Rd therapy improves histological and functional recovery in a rat model of inflammatory bowel disease. Phytother Res. 2020; 34(11): 3019-3028. https://doi.org/10.1002/ptr.6734
  • [27] Bidgoli SA, Taheri E, Ziarati P. Safety evaluation of oral Anethum graveolens L total hydroalcoholic extract in mice. Asian J Pharm Health Sci. 2012; 1(2): 61-67.
  • [28] Acute Oral Toxicity – Acute Toxic Class Method. OECD Guideline for Testing of Chemicals No. 423. www.oecd.org/env/testguidelines (accessed 2022 Oct 18).
Toplam 28 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Temel Farmakoloji
Bölüm Articles
Yazarlar

Karthick Kannan Rajendran 0009-0008-8329-4652

Indumathy Indumathy 0009-0005-9556-6000

Yayımlanma Tarihi 28 Haziran 2025
Yayımlandığı Sayı Yıl 2024 Cilt: 28 Sayı: 3

Kaynak Göster

APA Rajendran, K. K., & Indumathy, I. (2025). Therapeutic effect of Anethum graveolens L. (Dill) seed extract for the treatment of dextran sodium sulfate (DSS) induced ulcerative colitis in Wistar rats. Journal of Research in Pharmacy, 28(3), 770-780.
AMA Rajendran KK, Indumathy I. Therapeutic effect of Anethum graveolens L. (Dill) seed extract for the treatment of dextran sodium sulfate (DSS) induced ulcerative colitis in Wistar rats. J. Res. Pharm. Haziran 2025;28(3):770-780.
Chicago Rajendran, Karthick Kannan, ve Indumathy Indumathy. “Therapeutic Effect of Anethum Graveolens L. (Dill) Seed Extract for the Treatment of Dextran Sodium Sulfate (DSS) Induced Ulcerative Colitis in Wistar Rats”. Journal of Research in Pharmacy 28, sy. 3 (Haziran 2025): 770-80.
EndNote Rajendran KK, Indumathy I (01 Haziran 2025) Therapeutic effect of Anethum graveolens L. (Dill) seed extract for the treatment of dextran sodium sulfate (DSS) induced ulcerative colitis in Wistar rats. Journal of Research in Pharmacy 28 3 770–780.
IEEE K. K. Rajendran ve I. Indumathy, “Therapeutic effect of Anethum graveolens L. (Dill) seed extract for the treatment of dextran sodium sulfate (DSS) induced ulcerative colitis in Wistar rats”, J. Res. Pharm., c. 28, sy. 3, ss. 770–780, 2025.
ISNAD Rajendran, Karthick Kannan - Indumathy, Indumathy. “Therapeutic Effect of Anethum Graveolens L. (Dill) Seed Extract for the Treatment of Dextran Sodium Sulfate (DSS) Induced Ulcerative Colitis in Wistar Rats”. Journal of Research in Pharmacy 28/3 (Haziran 2025), 770-780.
JAMA Rajendran KK, Indumathy I. Therapeutic effect of Anethum graveolens L. (Dill) seed extract for the treatment of dextran sodium sulfate (DSS) induced ulcerative colitis in Wistar rats. J. Res. Pharm. 2025;28:770–780.
MLA Rajendran, Karthick Kannan ve Indumathy Indumathy. “Therapeutic Effect of Anethum Graveolens L. (Dill) Seed Extract for the Treatment of Dextran Sodium Sulfate (DSS) Induced Ulcerative Colitis in Wistar Rats”. Journal of Research in Pharmacy, c. 28, sy. 3, 2025, ss. 770-8.
Vancouver Rajendran KK, Indumathy I. Therapeutic effect of Anethum graveolens L. (Dill) seed extract for the treatment of dextran sodium sulfate (DSS) induced ulcerative colitis in Wistar rats. J. Res. Pharm. 2025;28(3):770-8.