Öz
Kaynakça
- [1] Yang Z. Natural Deep Eutectic Solvents and Their Applications in Biotechnology. In: T. I, Koo. Y, editors. Application of Ionic Liquids in Biotechnology Advances in Biochemical Engineering/Biotechnology. Springer, Cham; 2018. p. 31–59. [CrossRef]
- [2] Espino M, Fernández MDLÁ, Gomez FJ V, Silva MF. Natural Designer Solvents for Greening Analytical Chemistry. Trends Anal Chem. 2016; 76: 126-136. [CrossRef]
- [3] Badan Pengawas Obat dan Makanan RI. Acuan Sediaan Herbal Volume 6 Edisi Pertama. 2011. p 87–89.
- [4] Nirmal NP, Rajput MS, Prasad RGSV, Ahmad M. Brazilin from Caesalpinia sappan heartwood and its pharmacological activities: A review. Asian Pac J Trop Med. 2015; 8(6): 421–30. [CrossRef]
- [5] Lambeir A, Durinx C, Scharpé S. Dipeptidyl-Peptidase IV from Bench to Bedside : An Update on Structural Properties , Functions , and Clinical Aspects of the Enzyme DPP IV. 2003; 40(3): 209–94. [CrossRef]
- [6] Setyaningsih EP, Saputri FC, Mun’im A. The Antidiabetic Effectivity of Indonesian Plants Extracts via DPP-IV Inhibitory Mechanism. J Young Pharm. 2019; 11(2): 161–4.
- [7] International Conference on Harmonization. Validation of Analytical Procedures: Text and Methodology, Q2 (R1). Geneva; 2005.
- [8] AOAC International. AOAC Guidelines for Single Laboratory Validation of Chemical Methods for Dietary Supplements and Botanicals. Arlington: Association of Official Analytical Chemists; 2002.
- [9] Maran JP, Manikandan S, Thirugnanasambandham K, Nivetha CV, Dinesh R. Box – Behnken design based statistical modeling for ultrasound-assisted extraction of corn silk polysaccharide. Carbohydr Polym [Internet]. 2013; 92(1): 604–11. [CrossRef] [10] Bi W, Tian M, Ho K. Evaluation of alcohol-based deep eutectic solvent in extraction and determination of flavonoids with response surface methodology optimization. J Chromatogr A. 2013; 1285: 22–30. [CrossRef]
- [11] Ye L. Synthetic Strategies in Molecular Imprinting. In: Mattiasson B, Ye L, editors. Molecularly Imprinted Polymers in Biotechnology Advances in Biochemical Engineering/Biotechnology, vol 150. Springer, Cham; 2015. p. 1–24. [CrossRef]
- [12] Kim B, Kim HY, Choi I, Kim J, Jin CH, Han A. DPP-IV Inhibitory Potentials of Flavonol Glycosides Molecular Docking Analyses. Molecules. 2018; 23(8): 1-10. [CrossRef]
- [13] Khodaverdian S, Dabirmanesh B, Heydari A, Dashtban-moghadam E, Khajeh K, Ghazi F. Activity , stability and structure of laccase in betaine based natural deep eutectic solvents. Int J Biol Macromol. 2018; 107(B): 2574-2579. [CrossRef]
- [14] Dai Y, Spronsen J Van, Witkamp G. Natural deep eutectic solvents as new potential media for green technology. Anal Chim Acta. 2013; 766:61–8. [CrossRef]
- [15] Tuekaew J, Siriwatanametanon N, Wongkrajang Y, Temsiririrkkul R, Jantan I. Evaluation of the antioxidant activities of Ya-hom intajak, A thai herbal formulation, and its component plants. Trop J Pharm Res. 2014; 13(9): 1477–85. [CrossRef]
Application of Natural Deep Eutectic Solvents (NADES) for Sappan Wood (Caesalpinia sappan L.) extraction to test for inhibition of DPP IV activity
Öz
Some natural deep eutectic solvents (NADES) can replace organic solvents that are toxic and harmful to the environment. The main flavonoid in sappan wood (Caesalpinia sappan L.) is brazilin. The aims of this study were to extract brazilin from sappan wood by using NADES and to evaluate the effect of the extract on inhibition of dipeptidyl peptidase IV (DPP IV) activity. The selected NADES compositions used choline chloride and betaine as hydrogen bond acceptors (HBA) with glycerol, xylitol, sorbitol, lactic acid, malic acid, and citric acid as various hydrogen bond donors (HBD). Ultrasound-assisted NADES extraction of brazilin was performed, and the content was measured by highperformance liquid chromatography (HPLC). Between selected NADES, betain–lactic acid gave the highest brazilin content of 4.49 mg/g. This result was comparable to conventional methods by a reflux using 96% ethanol that gave brazilin content of 5.43 mg/g. However, the reflux required 3 hours for three extraction cycle, while optimal extraction conditions by betain–lactic acid were 30 min. The sappan wood extract using choline chloride–lactic acid had a percent inhibition of 5.72% (2.03 ppm), and that using betaine–lactic acid had a percent inhibition of 7.74% (2.08 ppm) for DPP IV activity. These inhibitory activities were comparable to the brazilin standard which was 8.93% (2.2 ppm). Our results showed that two NADES compositions could extract brazilin from sappan wood and it showed potential DPP IV inhibitory activity.
Anahtar Kelimeler
Brazilin Caesalpinia sappan L dipeptidyl peptidase IV natural deep eutectic solvents ultrasoundassisted extraction
Kaynakça
- [1] Yang Z. Natural Deep Eutectic Solvents and Their Applications in Biotechnology. In: T. I, Koo. Y, editors. Application of Ionic Liquids in Biotechnology Advances in Biochemical Engineering/Biotechnology. Springer, Cham; 2018. p. 31–59. [CrossRef]
- [2] Espino M, Fernández MDLÁ, Gomez FJ V, Silva MF. Natural Designer Solvents for Greening Analytical Chemistry. Trends Anal Chem. 2016; 76: 126-136. [CrossRef]
- [3] Badan Pengawas Obat dan Makanan RI. Acuan Sediaan Herbal Volume 6 Edisi Pertama. 2011. p 87–89.
- [4] Nirmal NP, Rajput MS, Prasad RGSV, Ahmad M. Brazilin from Caesalpinia sappan heartwood and its pharmacological activities: A review. Asian Pac J Trop Med. 2015; 8(6): 421–30. [CrossRef]
- [5] Lambeir A, Durinx C, Scharpé S. Dipeptidyl-Peptidase IV from Bench to Bedside : An Update on Structural Properties , Functions , and Clinical Aspects of the Enzyme DPP IV. 2003; 40(3): 209–94. [CrossRef]
- [6] Setyaningsih EP, Saputri FC, Mun’im A. The Antidiabetic Effectivity of Indonesian Plants Extracts via DPP-IV Inhibitory Mechanism. J Young Pharm. 2019; 11(2): 161–4.
- [7] International Conference on Harmonization. Validation of Analytical Procedures: Text and Methodology, Q2 (R1). Geneva; 2005.
- [8] AOAC International. AOAC Guidelines for Single Laboratory Validation of Chemical Methods for Dietary Supplements and Botanicals. Arlington: Association of Official Analytical Chemists; 2002.
- [9] Maran JP, Manikandan S, Thirugnanasambandham K, Nivetha CV, Dinesh R. Box – Behnken design based statistical modeling for ultrasound-assisted extraction of corn silk polysaccharide. Carbohydr Polym [Internet]. 2013; 92(1): 604–11. [CrossRef] [10] Bi W, Tian M, Ho K. Evaluation of alcohol-based deep eutectic solvent in extraction and determination of flavonoids with response surface methodology optimization. J Chromatogr A. 2013; 1285: 22–30. [CrossRef]
- [11] Ye L. Synthetic Strategies in Molecular Imprinting. In: Mattiasson B, Ye L, editors. Molecularly Imprinted Polymers in Biotechnology Advances in Biochemical Engineering/Biotechnology, vol 150. Springer, Cham; 2015. p. 1–24. [CrossRef]
- [12] Kim B, Kim HY, Choi I, Kim J, Jin CH, Han A. DPP-IV Inhibitory Potentials of Flavonol Glycosides Molecular Docking Analyses. Molecules. 2018; 23(8): 1-10. [CrossRef]
- [13] Khodaverdian S, Dabirmanesh B, Heydari A, Dashtban-moghadam E, Khajeh K, Ghazi F. Activity , stability and structure of laccase in betaine based natural deep eutectic solvents. Int J Biol Macromol. 2018; 107(B): 2574-2579. [CrossRef]
- [14] Dai Y, Spronsen J Van, Witkamp G. Natural deep eutectic solvents as new potential media for green technology. Anal Chim Acta. 2013; 766:61–8. [CrossRef]
- [15] Tuekaew J, Siriwatanametanon N, Wongkrajang Y, Temsiririrkkul R, Jantan I. Evaluation of the antioxidant activities of Ya-hom intajak, A thai herbal formulation, and its component plants. Trop J Pharm Res. 2014; 13(9): 1477–85. [CrossRef]
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Eczacılık ve İlaç Bilimleri (Diğer) |
| Bölüm | Articles |
| Yazarlar | |
| Yayımlanma Tarihi | 27 Haziran 2025 |
| Yayımlandığı Sayı | Yıl 2020 Cilt: 24 Sayı: 3 |