Öz
In this study, the cytotoxicity potential and antibacterial and antifungal activities of two different extracts
of fresh leaves of Arum italicum Miller were investigated. The saponin extract and the alkaloid extract were investigated
by Brine Shrimp (Artemia salina L.) lethality bioassay method for evaluation of cytotoxic effect. Umbelliferone was used
as standard, and both of the extracts showed cytotoxic effect by the Brine Shrimp (Artemia salina L.) lethality bioassay
method. According to the LC50 results, saponin extract was found to be the most active extract, and classifiable as
moderate to highly toxic (LC50 237.1437 mg/mL-1). Although, the alkaloid extract was showed cytotoxic activity lower
than Umbelliferone, LC50 750.1920 mg/mL-1 value was classifiable as mildly cytotoxic. Antibacterial and antifungal
activities were tested by the agar well diffusion and tube dilution methods. 6 bacteria and 6 yeasts were used in the
methods Meropenem and Fluconazole were the standards of the methods. The saponin extract was found to be effective
against Staphylococcus epidermidis and Sthaphylococcus aureus compared with Meropenem. Both of the extracts were not
found to be effective against fluconazole as antifungal activity point of view. According to the all studied activity results
the saponin contents of the fresh leaves of the plant emphasizing the antimicrobial activity. And, both of the extracts
have cytotoxic activity potential.
Anahtar Kelimeler
Arum italicum Miller saponin alkaloid antibacterial activity antifungal activity
Kaynakça
- [1] Davis PH, Flora of Turkey and the East Aegean Islands, Edinburgh University Pres, England, 1965.
- [2] Hacıoğlu M, Kulaksız B, Alpınar K, Hacıosmanoğlu E, Tan SB. In vitro antimicrobial, antibiofilm and cytotoxic activities of the extracts of Arum italicum Miller leaves. Farmacia. 2021; 69(5): 849-854. [CrossRef]
- [3] Baytop T, Theraphy with Medicinal Plants in Turkey (past and present) İstanbul University Publication, Türkiye, 1984. [4] PDR for herbal Medicines. Medicinal Economics Company, New Jersey, 2000.
- [5] Azab A. Arum: A plant genus with great medicinal potential. Eur Chem Bull. 2017; 6(2): 59-68. [CrossRef]
- [6] Ağalar HZ, Çiftçi GA, Göger F, Kırımer N. Activity guided fractionation of Arum italicum Miller tubers and the LC/MS-MS profiles. Rec Nat Prod. 2018; 12(1): 64-75. [CrossRef]
- [7] Gibernau M, Macquart D, Przetak G. Pollination in the Genus Arum – a review. Aroideana. 2004; 27: 148-166.
- [8] Nguta JM, Mbaria JM, Gathumbi PK, Kabasa JD, Kiama SG. Biological screening of Kenya medicinal plants using artemia salina (ARTEMIIDAE). Pharmacologyonline. 2011; 2: 458-478. [CrossRef]
- [9] Dey PM, Harborner JB, Methods in Plant Biochemistry, San Diego: Academic Press, New York,USA, 1991.
- [10] Kolak U, Ulusoylu M. Constituents of Delphinium uncinatum. Scientia Pharmaceutica. 1998; 66(4): 381-385. [CrossRef]
- [11] Çetin ET, General and Practic Microbiology, Sermet Press, Istanbul, 1973.
- [12] National Comittee for clinical Laboratory Standards, Reference Method for Broth Dilution Susuceptibility Testing of Yeast: Propsed Standard M27-P, National Committee for Clinical Laboratory Standards, Villanova, PA, 1992
- [13] National Comittee for clinical Laboratory Standards, Reference Method for Dilution Antimicrobial Susuceptibility Tests for Bacteria That Grow Aerobically, Approved Standard M7-A3, National Committee for Clinical Laboratory Standards, Villanova, PA, 1993.
- [14] Finney DJ, Probit analysis, Cambridge University Press, New York, 1971.
Öz
Kaynakça
- [1] Davis PH, Flora of Turkey and the East Aegean Islands, Edinburgh University Pres, England, 1965.
- [2] Hacıoğlu M, Kulaksız B, Alpınar K, Hacıosmanoğlu E, Tan SB. In vitro antimicrobial, antibiofilm and cytotoxic activities of the extracts of Arum italicum Miller leaves. Farmacia. 2021; 69(5): 849-854. [CrossRef]
- [3] Baytop T, Theraphy with Medicinal Plants in Turkey (past and present) İstanbul University Publication, Türkiye, 1984. [4] PDR for herbal Medicines. Medicinal Economics Company, New Jersey, 2000.
- [5] Azab A. Arum: A plant genus with great medicinal potential. Eur Chem Bull. 2017; 6(2): 59-68. [CrossRef]
- [6] Ağalar HZ, Çiftçi GA, Göger F, Kırımer N. Activity guided fractionation of Arum italicum Miller tubers and the LC/MS-MS profiles. Rec Nat Prod. 2018; 12(1): 64-75. [CrossRef]
- [7] Gibernau M, Macquart D, Przetak G. Pollination in the Genus Arum – a review. Aroideana. 2004; 27: 148-166.
- [8] Nguta JM, Mbaria JM, Gathumbi PK, Kabasa JD, Kiama SG. Biological screening of Kenya medicinal plants using artemia salina (ARTEMIIDAE). Pharmacologyonline. 2011; 2: 458-478. [CrossRef]
- [9] Dey PM, Harborner JB, Methods in Plant Biochemistry, San Diego: Academic Press, New York,USA, 1991.
- [10] Kolak U, Ulusoylu M. Constituents of Delphinium uncinatum. Scientia Pharmaceutica. 1998; 66(4): 381-385. [CrossRef]
- [11] Çetin ET, General and Practic Microbiology, Sermet Press, Istanbul, 1973.
- [12] National Comittee for clinical Laboratory Standards, Reference Method for Broth Dilution Susuceptibility Testing of Yeast: Propsed Standard M27-P, National Committee for Clinical Laboratory Standards, Villanova, PA, 1992
- [13] National Comittee for clinical Laboratory Standards, Reference Method for Dilution Antimicrobial Susuceptibility Tests for Bacteria That Grow Aerobically, Approved Standard M7-A3, National Committee for Clinical Laboratory Standards, Villanova, PA, 1993.
- [14] Finney DJ, Probit analysis, Cambridge University Press, New York, 1971.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Farmasotik Mikrobiyoloji |
| Bölüm | Articles |
| Yazarlar | |
| Yayımlanma Tarihi | 28 Haziran 2025 |
| Yayımlandığı Sayı | Yıl 2022 Cilt: 26 Sayı: 4 |