Araştırma Makalesi
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Yıl 2022, Cilt: 26 Sayı: 6, 1868 - 1876, 28.06.2025

Md. Abdul Halim Md. Mamunur Rashid Md. Abdul Mazid A. H. M. Mazbah Uddin Mohammad Salim Hossain Md. Mamun Or Rashid

https://doi.org/10.29228/jrp.276
Cited By: 1

Öz

Kaynakça

  • [1] Suba V, Murugesan T, Rao RB, Pal M, Mandal SC, Saha BP. Neuropharmacological profile of Barleria lupulinalindle extract in animal models. J Ethnopharmacol. 2002;81:251-255. [CrossRef]
  • [2] Dhawan K, Dhavan S, Chhabra S. Attenuation of benzodiazepine dependence in mice by a Trisubstituted benzoflavone moiety of Passiflora incarnate Linneous: A non habit forming anxiolytic. J Pharm Pharm Sci. 2003;6(2):215-222.
  • [3] Chatterjee SK, Bhattacharjee I, Chandra G. Isolation and identification of bioactive antibacterial components in leaf extracts of Vangueria spinosa (Rubiaceae). Asian Pac J Trop Med. 2011;4(1):35-40. [CrossRef]
  • [4] Karchesy YM, Kelsey RG, Constantine G, Karchesy JJ. Biological screening of selected Pacific Northwest forest plants using the brine shrimp (Artemia salina) toxicity bioassay. SpringerPlus. 2016;5:510. [CrossRef]
  • [5] Apu AS, Muhit MA, Tareq SM, Pathan AH, Jamaluddin ATM, Ahmed M. Antimicrobial activity and brine shrimp lethality bioassay of the leaves extract of Dillenia indica Linn. J Young Pharm. 2010;2(1):50-53. [CrossRef]
  • [6] Soberon JR, Sgariglia MA, Sampietro DA, Quiroga EN, Vottuone MA. Antibacterial activities of plant extracts from northwestern Argentina. J Appl Microbiol. 2007;102:1450-1461. [CrossRef]
  • [7] Kumar AR, Subburathinam KM, Prabakar G. Phytochemical screening of selected medicinal plants of Asclepiadaceae family. Asian J Microbiol Biotechnol Environ Sci. 2007;9(1):177-180.
  • [8] Patra JK, Dhal NK, Thatoi HN. In vitro bioactivity and phytochemical screening of Suaeda maritima (Dumort): A mangrove associate from Bhitarkanika, India. Asian Pac J Trop Med. 2011;4(9):727-734. [CrossRef]
  • [9] Ravikumar S, Gnanadesigan M, Inbaneson SJ, Kalaiarasi A. Hepatoprotective and antioxidant properties of Suaeda maritima (L.) Dumort ethanolic extraction concananalin-A induced hepatotoxicity in rats. Indian J Exp Biol. 2011;49(6):455-460.
  • [10] Alam MA, Slahin N, Uddin R, Hasan SMR, Akter R, Kamaluddin M, Faroque A, Ghani A. Analgesic and neuropharmacological investigations of the aerial part of Achyranthes aspera Linn. Stamford J Pharm Sci. 2008;1:44-50. [CrossRef]
  • [11] Ozturk Y, Aydini S, Beis R, Baser KHC, Berberoglu H. Effect of Hypericum pericum L. and Hypericum calycinum L. extracts on the central nervous system in mice. Phytomedicine. 1996;3(2):139-146. [CrossRef]
  • [12] Nwafor PA, Okwuasaba F. Anti-nociceptive and anti-inflammatory effects of methanolic extract of Asparagus pubescens root in rodents. J Ethnopharmacol. 2003;84:125–129. [CrossRef]
  • [13] Chirumbolo S. Gelsemine and Gelsemium sempervirens L. extracts in animal behavioral test: comments and related biases. Front Neurol. 2011;2:31. [CrossRef]
  • [14] Khatun A, Rahman M, Rahman MM, Hossain H, Jahan IA, Nesa ML. Antioxidant, antinociceptive and CNS activities of Viscum orientale and high sensitive quantification of bioactive polyphenols by UPLC. Front Pharmacol. 2016;7:176. [CrossRef]
  • [15] Bhattacharya SK, Satyan KS. Experimental methods for evaluation of psychotropic agents in rodents: Anti-anxiety agents. Indian J Exp Biol. 1997;35(6):565-575.
  • [16] Kolawole OT, Makinde JM, Olajide OA. Central nervous depressant activity of Russelia equisetiformis. Niger J Physiol Sci. 2007;22(1-2):59-63. [CrossRef]
  • [17] Longanga Otshudi A, Vercruysse A, Foriers A. Contribution to the ethnobotanical, phytochemical and pharmacological studies of traditionally used medicinal plants in the treatment of dysentery and diarrhoea in Lomela area, Democratic Republic of Congo (DRC). J Ethnopharmacol. 2000;71(3):411-423. [CrossRef]
  • [18] Sarker MA, Banik S, Hussain MS, Ghosh A, Hossain MS. In-vitro and In-vivo pharmacological activities with phytochemical evaluation of methanolic extract of Microcos paniculata stem barks. Curr Drug Ther. 2016;11:142-149. [CrossRef]
  • [19] Carlo GD, Mascolo N, Izzo AA, Capasso F, Autore G. Effect of querecetine on the gastrointestinal tract in rats and mice. Phytother Res. 1994;8:42-45. [CrossRef]
  • [20] Heinrich M, Rimpler H, Barrera NA. Indigenous phytotherapy of gastrointestinal disorders in a lowland mixe community (Oaxaco, Mexico): Ethnopharmacologic evaluation. J Ethnopharmacol. 1992;36(1):63-80. [CrossRef]
  • [21] Umaru IJ, Badruddin FA, Assim ZB, Umaru HA. Cytotoxicity (brine shrimp lethality bioassay) of Barringtonia racemosa leaves, stem-bark and root extract. J Biotechnol Bioeng. 2018;2(2):45-50.
  • [22] Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DE, McLaughlin JL. Brine shrimp: A convenient general bioassay for active plant constituents. Planta Medica. 1982;45:31-34.
  • [23] Chethankumara GP, Nagaraj K, Krishna V. In vitro cytotoxic potential of alkaloid and flavonoid rich fractions of Alseodaphne semecarpifolia against MCF-7 cells. Biomed Pharmacol J. 2021;14(2):557-565. [CrossRef]
  • [24] Rajabalian S, Mohammadi M, Mozaffari B. Cytotoxicity evaluation of Persica mouthwash on cultured human and mouse cell lines in the presence and absence of fetal calf serum. Indian J Dent Res. 2009;20(2):169-173. [CrossRef]
  • [25] Liu F, Tan X, Han X, Li X, Li N, Kang W. Cytotoxicity of Aconitum alkaloid and its interaction with calf thymus DNA by multi-spectroscopic techniques. Sci Rep. 2017;7:14509. [CrossRef]
  • [26] Pant DR, Pant ND, Saru DB, Yadav UN, Khanal DP. Phytochemical screening and study of antioxidant, antimicrobial, antidiabetic, anti-inflammatory and analgesic activities of extracts from stem wood of Pterocarpus marsupium Roxburgh. J Intercult Ethnopharmacol. 2017;6(2):170–176. [CrossRef]
  • [27] Shrestha P, Adhikari S, Lamichhane B, Shrestha BG. Phytochemical screening of the medicinal plants of Nepal. IOSR J Environ Sci Toxicol Food Technol. 2015;1(6):11-17.
  • [28] Shukla S, Mehta A, Bajpai VK. Phytochemical screening and anthelmintic and antifungal activities of leaf extracts of Stevia rebaudiana. J Biol Act Prod Nat. 2013;3(1):56-63. [CrossRef]
  • [29] Mondal S, Hossain I, Islam MN. Phytochemical screening of ethanolic extract of leaves and stems of Cucubita pepo Linn. Int J Chem Stud. 2017;1(2):32-34.
  • [30] Sharmin T, Sultana R, Hossain F, Shakil SK, Hossen F, Rashid MMO. Neuropharmacological and antibacterial effects of the ethyl acetate extract of Diospyros malabarica seeds. Clinical Phytoscience. 2018;4:22. [CrossRef]
  • [31] Mishra SK, Singh PN, Dubey SD. Evaluation of CNS depressant activity of Capparis zeylanica Lin. root. Res J Med Plants. 2011;5(6):738-746. [CrossRef]
  • [32] Tafesse G, Mekonnen Y. Anti-diarrheal activity of leaf extract of Juniperus procera and its effect on intestinal motility in albino mice. J Med Biomed Sci. 2012;1(4):7-12.

Central nervous system depressant, gastrointestinal motility, and brine shrimp lethality bioassay of the methanolic extract of Suaeda maritima in mice model

Yıl 2022, Cilt: 26 Sayı: 6, 1868 - 1876, 28.06.2025

Md. Abdul Halim Md. Mamunur Rashid Md. Abdul Mazid A. H. M. Mazbah Uddin Mohammad Salim Hossain Md. Mamun Or Rashid

https://doi.org/10.29228/jrp.276
Cited By: 1

Öz

Suaeda maritima (S. maritima) is a common yellow-green shrub grows in salt marshes. Present study aims to investigate the in-vivo central nervous system depressant, gastrointestinal motility and Brine shrimp lethality bioassay of the methanolic extract of S. maritima. Phytochemical screening was performed to observe the main constituents. Central nervous system depressant activity was evaluated through open field and hole cross tests separately. Gastrointestinal motility activity was observed using charcoal meal method. The extract was screened for cytotoxic activity using brine shrimp lethality bioassay. In open field method, the number of squares crossed by mice were 80.80 ± 1.66 and 80.60 ± 1.66 for 250 mg/kg and 500 mg/kg extract respectively at zero time, which reduced gradually to 26.00 ± 1.05 and 20.00 ± 0.95 after 60 minutes. Similar decreasing was found in hole cross method. Locomotor activities and exploratory behavior of mice were found to be decreased after the oral administration of S. maritima. On the other hand, S. maritima extract inhibit 62.9% and 69.7% gastrointestinal motility for 200 mg/kg and 400 mg/kg body weight of dose. In brine shrimp lethality test, LC50 values obtained from the experiment were 0.451 µg/µl and 0.254 µg/µl for S. maritima extract and positive control vincristine sulphate respectively. This study confirms the moderate cytotoxic effect of S. maritima that implies the presence of potent bioactive compounds. S. maritima possessed remarkable central nervous system depressant and gastrointestinal motility activities with moderate cytotoxic activity. Further investigation is warranted to find out the bioactive components with their exact mechanism of actions for drug development program.

Anahtar Kelimeler

Suaeda maritima central nervous system depressant hole cross gastrointestinal motility open field brineshrimp

Kaynakça

  • [1] Suba V, Murugesan T, Rao RB, Pal M, Mandal SC, Saha BP. Neuropharmacological profile of Barleria lupulinalindle extract in animal models. J Ethnopharmacol. 2002;81:251-255. [CrossRef]
  • [2] Dhawan K, Dhavan S, Chhabra S. Attenuation of benzodiazepine dependence in mice by a Trisubstituted benzoflavone moiety of Passiflora incarnate Linneous: A non habit forming anxiolytic. J Pharm Pharm Sci. 2003;6(2):215-222.
  • [3] Chatterjee SK, Bhattacharjee I, Chandra G. Isolation and identification of bioactive antibacterial components in leaf extracts of Vangueria spinosa (Rubiaceae). Asian Pac J Trop Med. 2011;4(1):35-40. [CrossRef]
  • [4] Karchesy YM, Kelsey RG, Constantine G, Karchesy JJ. Biological screening of selected Pacific Northwest forest plants using the brine shrimp (Artemia salina) toxicity bioassay. SpringerPlus. 2016;5:510. [CrossRef]
  • [5] Apu AS, Muhit MA, Tareq SM, Pathan AH, Jamaluddin ATM, Ahmed M. Antimicrobial activity and brine shrimp lethality bioassay of the leaves extract of Dillenia indica Linn. J Young Pharm. 2010;2(1):50-53. [CrossRef]
  • [6] Soberon JR, Sgariglia MA, Sampietro DA, Quiroga EN, Vottuone MA. Antibacterial activities of plant extracts from northwestern Argentina. J Appl Microbiol. 2007;102:1450-1461. [CrossRef]
  • [7] Kumar AR, Subburathinam KM, Prabakar G. Phytochemical screening of selected medicinal plants of Asclepiadaceae family. Asian J Microbiol Biotechnol Environ Sci. 2007;9(1):177-180.
  • [8] Patra JK, Dhal NK, Thatoi HN. In vitro bioactivity and phytochemical screening of Suaeda maritima (Dumort): A mangrove associate from Bhitarkanika, India. Asian Pac J Trop Med. 2011;4(9):727-734. [CrossRef]
  • [9] Ravikumar S, Gnanadesigan M, Inbaneson SJ, Kalaiarasi A. Hepatoprotective and antioxidant properties of Suaeda maritima (L.) Dumort ethanolic extraction concananalin-A induced hepatotoxicity in rats. Indian J Exp Biol. 2011;49(6):455-460.
  • [10] Alam MA, Slahin N, Uddin R, Hasan SMR, Akter R, Kamaluddin M, Faroque A, Ghani A. Analgesic and neuropharmacological investigations of the aerial part of Achyranthes aspera Linn. Stamford J Pharm Sci. 2008;1:44-50. [CrossRef]
  • [11] Ozturk Y, Aydini S, Beis R, Baser KHC, Berberoglu H. Effect of Hypericum pericum L. and Hypericum calycinum L. extracts on the central nervous system in mice. Phytomedicine. 1996;3(2):139-146. [CrossRef]
  • [12] Nwafor PA, Okwuasaba F. Anti-nociceptive and anti-inflammatory effects of methanolic extract of Asparagus pubescens root in rodents. J Ethnopharmacol. 2003;84:125–129. [CrossRef]
  • [13] Chirumbolo S. Gelsemine and Gelsemium sempervirens L. extracts in animal behavioral test: comments and related biases. Front Neurol. 2011;2:31. [CrossRef]
  • [14] Khatun A, Rahman M, Rahman MM, Hossain H, Jahan IA, Nesa ML. Antioxidant, antinociceptive and CNS activities of Viscum orientale and high sensitive quantification of bioactive polyphenols by UPLC. Front Pharmacol. 2016;7:176. [CrossRef]
  • [15] Bhattacharya SK, Satyan KS. Experimental methods for evaluation of psychotropic agents in rodents: Anti-anxiety agents. Indian J Exp Biol. 1997;35(6):565-575.
  • [16] Kolawole OT, Makinde JM, Olajide OA. Central nervous depressant activity of Russelia equisetiformis. Niger J Physiol Sci. 2007;22(1-2):59-63. [CrossRef]
  • [17] Longanga Otshudi A, Vercruysse A, Foriers A. Contribution to the ethnobotanical, phytochemical and pharmacological studies of traditionally used medicinal plants in the treatment of dysentery and diarrhoea in Lomela area, Democratic Republic of Congo (DRC). J Ethnopharmacol. 2000;71(3):411-423. [CrossRef]
  • [18] Sarker MA, Banik S, Hussain MS, Ghosh A, Hossain MS. In-vitro and In-vivo pharmacological activities with phytochemical evaluation of methanolic extract of Microcos paniculata stem barks. Curr Drug Ther. 2016;11:142-149. [CrossRef]
  • [19] Carlo GD, Mascolo N, Izzo AA, Capasso F, Autore G. Effect of querecetine on the gastrointestinal tract in rats and mice. Phytother Res. 1994;8:42-45. [CrossRef]
  • [20] Heinrich M, Rimpler H, Barrera NA. Indigenous phytotherapy of gastrointestinal disorders in a lowland mixe community (Oaxaco, Mexico): Ethnopharmacologic evaluation. J Ethnopharmacol. 1992;36(1):63-80. [CrossRef]
  • [21] Umaru IJ, Badruddin FA, Assim ZB, Umaru HA. Cytotoxicity (brine shrimp lethality bioassay) of Barringtonia racemosa leaves, stem-bark and root extract. J Biotechnol Bioeng. 2018;2(2):45-50.
  • [22] Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DE, McLaughlin JL. Brine shrimp: A convenient general bioassay for active plant constituents. Planta Medica. 1982;45:31-34.
  • [23] Chethankumara GP, Nagaraj K, Krishna V. In vitro cytotoxic potential of alkaloid and flavonoid rich fractions of Alseodaphne semecarpifolia against MCF-7 cells. Biomed Pharmacol J. 2021;14(2):557-565. [CrossRef]
  • [24] Rajabalian S, Mohammadi M, Mozaffari B. Cytotoxicity evaluation of Persica mouthwash on cultured human and mouse cell lines in the presence and absence of fetal calf serum. Indian J Dent Res. 2009;20(2):169-173. [CrossRef]
  • [25] Liu F, Tan X, Han X, Li X, Li N, Kang W. Cytotoxicity of Aconitum alkaloid and its interaction with calf thymus DNA by multi-spectroscopic techniques. Sci Rep. 2017;7:14509. [CrossRef]
  • [26] Pant DR, Pant ND, Saru DB, Yadav UN, Khanal DP. Phytochemical screening and study of antioxidant, antimicrobial, antidiabetic, anti-inflammatory and analgesic activities of extracts from stem wood of Pterocarpus marsupium Roxburgh. J Intercult Ethnopharmacol. 2017;6(2):170–176. [CrossRef]
  • [27] Shrestha P, Adhikari S, Lamichhane B, Shrestha BG. Phytochemical screening of the medicinal plants of Nepal. IOSR J Environ Sci Toxicol Food Technol. 2015;1(6):11-17.
  • [28] Shukla S, Mehta A, Bajpai VK. Phytochemical screening and anthelmintic and antifungal activities of leaf extracts of Stevia rebaudiana. J Biol Act Prod Nat. 2013;3(1):56-63. [CrossRef]
  • [29] Mondal S, Hossain I, Islam MN. Phytochemical screening of ethanolic extract of leaves and stems of Cucubita pepo Linn. Int J Chem Stud. 2017;1(2):32-34.
  • [30] Sharmin T, Sultana R, Hossain F, Shakil SK, Hossen F, Rashid MMO. Neuropharmacological and antibacterial effects of the ethyl acetate extract of Diospyros malabarica seeds. Clinical Phytoscience. 2018;4:22. [CrossRef]
  • [31] Mishra SK, Singh PN, Dubey SD. Evaluation of CNS depressant activity of Capparis zeylanica Lin. root. Res J Med Plants. 2011;5(6):738-746. [CrossRef]
  • [32] Tafesse G, Mekonnen Y. Anti-diarrheal activity of leaf extract of Juniperus procera and its effect on intestinal motility in albino mice. J Med Biomed Sci. 2012;1(4):7-12.
Toplam 32 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Farmakognozi, Eczacılık ve İlaç Bilimleri (Diğer)
Bölüm Articles
Yazarlar

Md. Abdul Halim

Md. Mamunur Rashid

Md. Abdul Mazid

A. H. M. Mazbah Uddin

Mohammad Salim Hossain

Md. Mamun Or Rashid

Yayımlanma Tarihi 28 Haziran 2025
Yayımlandığı Sayı Yıl 2022 Cilt: 26 Sayı: 6

Kaynak Göster

APA Halim, M. A., Rashid, M. M., Mazid, M. A., Uddin, A. H. M. M., vd. (2025). Central nervous system depressant, gastrointestinal motility, and brine shrimp lethality bioassay of the methanolic extract of Suaeda maritima in mice model. Journal of Research in Pharmacy, 26(6), 1868-1876. https://doi.org/10.29228/jrp.276
AMA Halim MA, Rashid MM, Mazid MA, Uddin AHMM, Hossain MS, Rashid MMO. Central nervous system depressant, gastrointestinal motility, and brine shrimp lethality bioassay of the methanolic extract of Suaeda maritima in mice model. J. Res. Pharm. Haziran 2025;26(6):1868-1876. doi:10.29228/jrp.276
Chicago Halim, Md. Abdul, Md. Mamunur Rashid, Md. Abdul Mazid, A. H. M. Mazbah Uddin, Mohammad Salim Hossain, ve Md. Mamun Or Rashid. “Central Nervous System Depressant, Gastrointestinal Motility, and Brine Shrimp Lethality Bioassay of the Methanolic Extract of Suaeda Maritima in Mice Model”. Journal of Research in Pharmacy 26, sy. 6 (Haziran 2025): 1868-76. https://doi.org/10.29228/jrp.276.
EndNote Halim MA, Rashid MM, Mazid MA, Uddin AHMM, Hossain MS, Rashid MMO (01 Haziran 2025) Central nervous system depressant, gastrointestinal motility, and brine shrimp lethality bioassay of the methanolic extract of Suaeda maritima in mice model. Journal of Research in Pharmacy 26 6 1868–1876.
IEEE M. A. Halim, M. M. Rashid, M. A. Mazid, A. H. M. M. Uddin, M. S. Hossain, ve M. M. O. Rashid, “Central nervous system depressant, gastrointestinal motility, and brine shrimp lethality bioassay of the methanolic extract of Suaeda maritima in mice model”, J. Res. Pharm., c. 26, sy. 6, ss. 1868–1876, 2025, doi: 10.29228/jrp.276.
ISNAD Halim, Md. Abdul vd. “Central Nervous System Depressant, Gastrointestinal Motility, and Brine Shrimp Lethality Bioassay of the Methanolic Extract of Suaeda Maritima in Mice Model”. Journal of Research in Pharmacy 26/6 (Haziran 2025), 1868-1876. https://doi.org/10.29228/jrp.276.
JAMA Halim MA, Rashid MM, Mazid MA, Uddin AHMM, Hossain MS, Rashid MMO. Central nervous system depressant, gastrointestinal motility, and brine shrimp lethality bioassay of the methanolic extract of Suaeda maritima in mice model. J. Res. Pharm. 2025;26:1868–1876.
MLA Halim, Md. Abdul vd. “Central Nervous System Depressant, Gastrointestinal Motility, and Brine Shrimp Lethality Bioassay of the Methanolic Extract of Suaeda Maritima in Mice Model”. Journal of Research in Pharmacy, c. 26, sy. 6, 2025, ss. 1868-76, doi:10.29228/jrp.276.
Vancouver Halim MA, Rashid MM, Mazid MA, Uddin AHMM, Hossain MS, Rashid MMO. Central nervous system depressant, gastrointestinal motility, and brine shrimp lethality bioassay of the methanolic extract of Suaeda maritima in mice model. J. Res. Pharm. 2025;26(6):1868-76.

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