Araştırma Makalesi
Evaluation of the teratogenic effect of water extract of Sonchus arvensis L. and Anredera cordifolia (Ten.) Steenis leaves as a combination in Wistar rat
Öz
Water extract of Sonchus arvensis and Anredera cordifolia leaves as a combination has been proven for their
antihypertensive activity, but there is no information about its safety for pregnant women yet. This experiment aimed
to evaluate the teratogenic effect of water extract of S. arvensis and A. cordifolia leaves as a combination (1:1) at doses of
100 mg/kg bw, 400 mg/kg bw, and 1000 mg/kg bw in Wistar rats. The extract was made into suspension in sodium
carboxymethyl cellulose (Na-CMC) 0.5% and given orally to pregnant rats on gestation day 6-15 as much as 1 mL/100
gram bw. Pregnant rats were sacrificed at gestation day 19. All parameters related to fetuses included the number of
fetuses, fetal weight, skeletal and organ malformation were evaluated. The percentage of skeletal and organ
malformation of each treatment groups was compared to the control group and analyzed with one-way ANOVA
method using IBM SPSS 28. There was no significant difference in both average number and fetal weight from all
treatment groups compared to the control group (P>0.05). Skeletal malformation was not found, but there were
undeveloped fetuses (resorption) and some fetuses with small brains in all groups. The percentage of each treatment
groups for those two conditions was not significantly different compared to the control group. Therefore, water extract
of S. arvensis and A. cordifolia leaves as a combination at doses of 100 mg/kg bw, 400 mg/kg bw, and 1000 mg/kg bw
contraindicated for pregnant women and those who are planning on pregnancy.
Anahtar Kelimeler
S.arvensis A.cordifolia water leaves extract antihypertensive activity teratogenic effect
Kaynakça
- Salim Z, Munadi E, Info Komoditi Tanaman Obat, Badan Pengkajian dan Pengembangan Perdagangan Kementerian Perdagangan Republik Indonesia, Indonesia, 2017.
- Sukandar EY, Ayuningtyas DK. Anredera cordifolia (Ten.) Steenis: A New Emerging Cure with Polypharmacological Effect, in Ghost D, Mukherjee PK, ed; Natural Medicines: Clinical Efficacy, Safety and Quality, CRC Press Taylor & Francis Group, Florida.
- Dhianawaty D, Soediro I, Soemardji A. Two synergetic effects of Sonchus arvensis l . leaves decoction in calcium oxalate bladder stone therapy on male Wistar rat. 2012; 2(2): 147–9.
- Yuan T, Wu D, Sun K, Tan X, Wang J, Zhao T, Ren B, Zhao B, Liu Z, Liu, X. Anti-fatigue activity of aqueous extracts of sonchus arvensis L. In exercise trained mice. Molecules. 2019; 24(6): 5–7. [CrossRef]
- Suliska N, Suryani, Insanu M, Sukandar EY. Antihypertensive activity of combination of anredera cordifolia (Ten.) V. Steenis and Sonchus arvensis L. leaves on Epinephrine Induced male Wistar Rat. J Adv Pharm Technol Res. 2021; 12(4): 384-8. [CrossRef]
- Sukandar EY, Kurniati NF, Nurdianti AF. Antiobesity effect of ethanol extract of anredera cordifolia (ten) steenis leaves on obese male wistar rats induced by a high-carbohydrate diet. Int J Pharm Pharm Sci. 2016; 8(4): 171-3.
- Basyuni M and Lesmana I. Phytochemical analysis of Binahong (Anredera cordifolia) leaves extract inhibit In Vitro growth of Aeromonas Hydrophila. AIP Publishing. 2017; 1-5.
- Chairul SM, Sumarny R, Chairul. Aktivitas antioksidan ekstrak air daun tempuyung (Sonchus arvensis L.) secara in- vitro. Majalah Farmasi Inonesia. 2003; 14(4): 208–15.
- Khuluk RH, Yunita A, Rohaeti E, Syafitri UD, Linda R, Lim LW, Takeuchi T, Rafi M. An HPLC-DAD Method to Quantify Flavonoids in Sonchus arvensis and Able to Classify the Plant Parts and Their Geographical Area through Principal Component Analysis. Separations. 2021; 8(2): 1-10. [CrossRef]
- National High Blood Pressure Education Program Working Group, High blood pressure in pregnancy, 2000. Podymow T, August P. Antihypertensive Drugs in Pregnancy. Seminars in Nephrology. 2011; 31(1): 70–85. [CrossRef]
- Samadi A, Mayberry R, Zaidi A, Pleasant J, McGhee M, Rice R. Maternal hypertension and associated pregnancy complications among AfricanAmerican and other women in the United States. Obstet Gynecol. 1996; 87: 557–63. [CrossRef]
- Muhtadi, Wahyuningtyas N, Sutrisna EM, Suhendi A, Anggoro BKS. Efek Diuresis Dari Kombinasi Ekstrak Salam (Syzygium polyanthum Wight.) dan Tempuyung (Sonchus arvensis L.) pada Mencit Hiperurisemia. 4th Univ Res Coloquium 2016. 2016; 22–7.
- Sukandar EY, Ridwan A, Sukmawan YP, Vasodilatation effect of ethanolic extract of Anredera cordifolia, Sonchus arvensis L, and ursolic acid on isolated rabbit aortic and frog heart. Int J Pharm Pharm Sci. 2016; 8( 2): 145-9.
- Suryani, Sukandar EY, Sutjiatmo AB, Vikasari SN. Angiotensin Converting Enzyme Inhibitor Activity of Ethanol Extract of Sonchus Arvensis (Linn.) Leaves. In: The 6th International Conference. 2017. p. 124–128.
- Sukandar EY, Ridwan A, Sukmawan TP, Vasodilation effect of oleanolic acid and apigenin as a metabolite compound of Anredera cordifolia (Ten) V Steenis on isolated rabbit aortic and frog heart. Int.J.Res Ayurveda Pharm. 2016; 7(5): 82- 4. [CrossRef]
- Garmana AN, Sukandar EY, Fidrianny I . Preliminary study of blood pressure-lowering effect of anredera cordifolia (ten.) steenis) on Wistar rats. IJPPR. 2016; 8(2): 300-30.
- Vasant OK, Vijay BG, Virbhadrappa SR, Dilip NT, Ramahari MV, Laxamanrao BS. Antihypertensive and diuretic effects of the aqueous extract of Colocasia esculenta Linn. leaves in experimental paradigms. Iran J Pharm Res 2012; 11: 621-34.
- Sukandar EY, Garmana, AN, Aidasari AU , Crystalia AA. Antihypertensive activity of ethanol extract combination of Anredera cordifolia (Ten.) v. Steenis and Sonchus arvensis L. leaves on angiotensin II- induced male wistar rat. J Res Pharm. 2019; 23(6): 1090-97. [CrossRef]
- Sukandar EY, Safitri D, Evaluation of Teratogenic Effect of Tempuyung (Sonchus arvensis) Extract on Wistar Rats. IJPPR. 2016; 8(5): 761-6.
- Sukandar EY, Kurniati NF, Ftria V. Evaluation of teratogenicity effects of ethanolic extracts of binahong leaves (Anredera cordifolia (Ten.) Steenis in wistar rat. Int J Parm Pharm Sci. 2014; 6(11): 422-6.
- John Hopkins Univerisity. https://web.jhu.edu/animalcare/procedures/rat.html (accessed on 25 August 2021)
- Chahoud I, Franklin CB. Relationships between fetal body weight of Wistar rats at term and the extent of skeletal ossification. Braz J Med Biol Res. 2005; 38: 565–75. [CrossRef]
- Drews B, Landaverde LF, Kühl A, Drews U. Spontaneous embryo resorption in the mouse is triggered by embryonic apoptosis followed by rapid removal via maternal sterile purulent inflammation. BMC Dev Biol. 2020; 20(1): 1–18. [CrossRef]
- Dwornik JJ, Moore KL. Skeletal Malformations in the Holtzman Rat Embryo Following the Administration of Thalidomide. J Embryol Exp Morphol. 1965; 13(2): 181–93.
- Fadel RA, Sequeira RP, Abu-Hijleh MF, Obeidat M, Salem AHA. Effect of prenatal administration of therapeutic doses of topiramate on ossification of ribs and vertebrae in rat fetuses. Rom J Morphol Embryol. 2012; 53(2): 321–7.
- Hill M. Mouse Timeline Detailed. embryology.med.unsw.edu.au/embryology/index.php/Mouse_Timeline_Detailed (accessed 3 February 2021)
- Otis E, Brent R. Equivalent ages in mouse and human embryos. Anat Rec. 1954; 120(1): 33–63.
- Seely J. A Brief Review of Kidney Development, Maturation, Developmental Abnormalities, and Drug Toxicity: Juvenile Animal Relevancy. J Toxicol Pathol. 2017; 30(2): 125-33. [CrossRef]
Yıl 2023,
Cilt: 27 Sayı: 2, 762 - 768, 27.06.2025
Öz
Kaynakça
- Salim Z, Munadi E, Info Komoditi Tanaman Obat, Badan Pengkajian dan Pengembangan Perdagangan Kementerian Perdagangan Republik Indonesia, Indonesia, 2017.
- Sukandar EY, Ayuningtyas DK. Anredera cordifolia (Ten.) Steenis: A New Emerging Cure with Polypharmacological Effect, in Ghost D, Mukherjee PK, ed; Natural Medicines: Clinical Efficacy, Safety and Quality, CRC Press Taylor & Francis Group, Florida.
- Dhianawaty D, Soediro I, Soemardji A. Two synergetic effects of Sonchus arvensis l . leaves decoction in calcium oxalate bladder stone therapy on male Wistar rat. 2012; 2(2): 147–9.
- Yuan T, Wu D, Sun K, Tan X, Wang J, Zhao T, Ren B, Zhao B, Liu Z, Liu, X. Anti-fatigue activity of aqueous extracts of sonchus arvensis L. In exercise trained mice. Molecules. 2019; 24(6): 5–7. [CrossRef]
- Suliska N, Suryani, Insanu M, Sukandar EY. Antihypertensive activity of combination of anredera cordifolia (Ten.) V. Steenis and Sonchus arvensis L. leaves on Epinephrine Induced male Wistar Rat. J Adv Pharm Technol Res. 2021; 12(4): 384-8. [CrossRef]
- Sukandar EY, Kurniati NF, Nurdianti AF. Antiobesity effect of ethanol extract of anredera cordifolia (ten) steenis leaves on obese male wistar rats induced by a high-carbohydrate diet. Int J Pharm Pharm Sci. 2016; 8(4): 171-3.
- Basyuni M and Lesmana I. Phytochemical analysis of Binahong (Anredera cordifolia) leaves extract inhibit In Vitro growth of Aeromonas Hydrophila. AIP Publishing. 2017; 1-5.
- Chairul SM, Sumarny R, Chairul. Aktivitas antioksidan ekstrak air daun tempuyung (Sonchus arvensis L.) secara in- vitro. Majalah Farmasi Inonesia. 2003; 14(4): 208–15.
- Khuluk RH, Yunita A, Rohaeti E, Syafitri UD, Linda R, Lim LW, Takeuchi T, Rafi M. An HPLC-DAD Method to Quantify Flavonoids in Sonchus arvensis and Able to Classify the Plant Parts and Their Geographical Area through Principal Component Analysis. Separations. 2021; 8(2): 1-10. [CrossRef]
- National High Blood Pressure Education Program Working Group, High blood pressure in pregnancy, 2000. Podymow T, August P. Antihypertensive Drugs in Pregnancy. Seminars in Nephrology. 2011; 31(1): 70–85. [CrossRef]
- Samadi A, Mayberry R, Zaidi A, Pleasant J, McGhee M, Rice R. Maternal hypertension and associated pregnancy complications among AfricanAmerican and other women in the United States. Obstet Gynecol. 1996; 87: 557–63. [CrossRef]
- Muhtadi, Wahyuningtyas N, Sutrisna EM, Suhendi A, Anggoro BKS. Efek Diuresis Dari Kombinasi Ekstrak Salam (Syzygium polyanthum Wight.) dan Tempuyung (Sonchus arvensis L.) pada Mencit Hiperurisemia. 4th Univ Res Coloquium 2016. 2016; 22–7.
- Sukandar EY, Ridwan A, Sukmawan YP, Vasodilatation effect of ethanolic extract of Anredera cordifolia, Sonchus arvensis L, and ursolic acid on isolated rabbit aortic and frog heart. Int J Pharm Pharm Sci. 2016; 8( 2): 145-9.
- Suryani, Sukandar EY, Sutjiatmo AB, Vikasari SN. Angiotensin Converting Enzyme Inhibitor Activity of Ethanol Extract of Sonchus Arvensis (Linn.) Leaves. In: The 6th International Conference. 2017. p. 124–128.
- Sukandar EY, Ridwan A, Sukmawan TP, Vasodilation effect of oleanolic acid and apigenin as a metabolite compound of Anredera cordifolia (Ten) V Steenis on isolated rabbit aortic and frog heart. Int.J.Res Ayurveda Pharm. 2016; 7(5): 82- 4. [CrossRef]
- Garmana AN, Sukandar EY, Fidrianny I . Preliminary study of blood pressure-lowering effect of anredera cordifolia (ten.) steenis) on Wistar rats. IJPPR. 2016; 8(2): 300-30.
- Vasant OK, Vijay BG, Virbhadrappa SR, Dilip NT, Ramahari MV, Laxamanrao BS. Antihypertensive and diuretic effects of the aqueous extract of Colocasia esculenta Linn. leaves in experimental paradigms. Iran J Pharm Res 2012; 11: 621-34.
- Sukandar EY, Garmana, AN, Aidasari AU , Crystalia AA. Antihypertensive activity of ethanol extract combination of Anredera cordifolia (Ten.) v. Steenis and Sonchus arvensis L. leaves on angiotensin II- induced male wistar rat. J Res Pharm. 2019; 23(6): 1090-97. [CrossRef]
- Sukandar EY, Safitri D, Evaluation of Teratogenic Effect of Tempuyung (Sonchus arvensis) Extract on Wistar Rats. IJPPR. 2016; 8(5): 761-6.
- Sukandar EY, Kurniati NF, Ftria V. Evaluation of teratogenicity effects of ethanolic extracts of binahong leaves (Anredera cordifolia (Ten.) Steenis in wistar rat. Int J Parm Pharm Sci. 2014; 6(11): 422-6.
- John Hopkins Univerisity. https://web.jhu.edu/animalcare/procedures/rat.html (accessed on 25 August 2021)
- Chahoud I, Franklin CB. Relationships between fetal body weight of Wistar rats at term and the extent of skeletal ossification. Braz J Med Biol Res. 2005; 38: 565–75. [CrossRef]
- Drews B, Landaverde LF, Kühl A, Drews U. Spontaneous embryo resorption in the mouse is triggered by embryonic apoptosis followed by rapid removal via maternal sterile purulent inflammation. BMC Dev Biol. 2020; 20(1): 1–18. [CrossRef]
- Dwornik JJ, Moore KL. Skeletal Malformations in the Holtzman Rat Embryo Following the Administration of Thalidomide. J Embryol Exp Morphol. 1965; 13(2): 181–93.
- Fadel RA, Sequeira RP, Abu-Hijleh MF, Obeidat M, Salem AHA. Effect of prenatal administration of therapeutic doses of topiramate on ossification of ribs and vertebrae in rat fetuses. Rom J Morphol Embryol. 2012; 53(2): 321–7.
- Hill M. Mouse Timeline Detailed. embryology.med.unsw.edu.au/embryology/index.php/Mouse_Timeline_Detailed (accessed 3 February 2021)
- Otis E, Brent R. Equivalent ages in mouse and human embryos. Anat Rec. 1954; 120(1): 33–63.
- Seely J. A Brief Review of Kidney Development, Maturation, Developmental Abnormalities, and Drug Toxicity: Juvenile Animal Relevancy. J Toxicol Pathol. 2017; 30(2): 125-33. [CrossRef]
Toplam 28 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Temel Farmakoloji |
| Bölüm | Articles |
| Yazarlar | |
| Yayımlanma Tarihi | 27 Haziran 2025 |
| Yayımlandığı Sayı | Yıl 2023 Cilt: 27 Sayı: 2 |