Araştırma Makalesi
Determination of Vitamin D3 Loaded Self- nanoemulsifying Drug Delivery Systems (SNEDDS) Based Hydrogel
Öz
High Performance Liquid Chromatography (HPLC) was used to determine vitamin D3 content from the
topical dosage form. Self-nanoemulsifying drug delivery systems (SNEDDS) based hydrogel was aimed to optimize the
delivery of vitamin D3 through the skin due to its high lipophilicity. Quantifying vitamin D3 was performed using the
internal standard, vitamin D2, on LiChrocart C18 125-4 reverse phase column. Several characteristics of validation were
referred including specificity, linearity, range, accuracy, precision, limits of detection, and quantification. The mobile
phase was optimized as a mixture of acetonitrile-methanol-water for injection 94:3:3 v/v, and the flow rate was
optimized at 0.8 mL/min at 25°C. Vitamin D3 detection and quantification were observed at a wavelength of 266 nm
using an ultra-violet (UV) detector. The analysis method performed no excipient interference, including separating
vitamin D3 from its matrix. Extraction of vitamin D3 from its matrix was observed using the dialysis membrane, with
agitation (vortex) and no agitation (immersion). The drug content determination was found to be 108.22 % ± 14.77% for
the immersion method and 72.04% ± 33.37% for the vortex method. Further, the high variability of the results indicated
the dialysis membrane was not a suitable method for determining lipophilic drug content from the colloidal polymer
matrix. The other techniques with negligible variation, such as reverse dialysis or subsequent solvent addition, are
preferred.
Anahtar Kelimeler
Kaynakça
- Tripkovic L, Lambert H, Hart K, Smith CP, Bucca G, Penson S, Chope G, Elina H, Berry J, Vieth R, Lanham-New S. Comparison of vitamin D2 and vitamin D3 supplementation in raising serum 25-hydroxyvitamin D status: a systematic review and meta-analysis. Am J Clin Nutr. 2012;95(6):1357-1364. https://doi.org/10.3945/ajcn.111.031070
- Sirajudeen S, Shah I, Al Menhali A. A narrative role of vitamin D and its receptor: With current evidence on the gastric tissues. Int J Mol Sci. 2019;20(15):3832. https://doi.org/10.3390/ijms20153832
- Piotrowska A, Wierzbicka J, Zmijewski MA. Vitamin D in the skin physiology and pathology. Acta Biochim Pol. 2016;63(1):17-29.https://doi.org/10.18388/abp.2015_1104
- Bocheva G, Slominski RM, Slominski AT. The impact of vitamin d on skin aging. Int J Mol Sci. 2021;22(16):9097. https://doi.org/10.3390/ijms22169097
- Bi Y, Xia H, Li L, Lee RJ, Xie J, Liu Z, Qiu Z, Teng L. Liposomal vitamin D3 as an anti-aging agent for the skin. Pharmaceutics. 2019;11(7):311. https://doi.org/10.3390/pharmaceutics11070311
- Singh Y, Meher JG, Raval K, Khan FA, Chaurasia M, Jain NK, Chourasia MK. Nanoemulsion: Concepts, development and applications in drug delivery. J Control Release. 2017;252:28-49. https://doi.org/10.1016/j.jconrel.2017.03.008
- Devireddy SK, Jonnalagadda LP. A literature review on self nanoemulsifying drug delivery system (SNEDDS). Int J Pharm Sci Rev Res. 2021;70(1):85-94. https://doi.org/10.47583/ijpsrr.2021.v70i01.011
- Cholecalciferol. National Library of Medicine. https://pubchem.ncbi.nlm.nih.gov/compound/Cholecalciferol. (accessed July 4, 2022)
- Ponto T, Latter G, Luna G, Leite-Silva VR, Wright A, Benson HAE. Novel self-nano-emulsifying drug delivery systems containing astaxanthin for topical skin delivery. Pharmaceutics. 2021;13(5):649. https://doi.org/10.3390/pharmaceutics13050649
- Ravisankar P, Naga Navya C, Pravallika D, Sri DN. A review on step-by-step analytical method validation. IOSR J Pharm. 2015;5(10):2250-3013.
- European Medicines Agency. ICH Topic Q 2 (R1) Validation of Analytical Procedures: Text and Methodology. London: European Medicines Agency; 2006.
- Miller JN, Miller JC. Statistics and chemometrics for analytical chemistry. Sixth. London: Pearson Education Limited; 2010. 119-127.
- AOAC International. Appendix F: Guidelines for Standard Method Performance Requirements. AOAC Int Off Method Anal. 2016;1-18.
- Williams RO, Sykora MA, Mahaguna V. Method to recover a lipophilic drug from hydroxypropyl methylcellulose matrix tablets. AAPS PharmSciTech. 2001;2(2):E8. https://doi.org/10.1208/pt020208
- Knoke S, Bunjes H. Transfer of lipophilic drugs from nanoemulsions into lipid-containing alginate microspheres. Pharmaceutics. 2021;13(2):173. https://doi.org/10.3390/pharmaceutics13020173
- Xu X, Khan MA, Burgess DJ. A two-stage reverse dialysis in vitro dissolution testing method for passive targeted liposomes. Int J Pharm. 2012;426(1-2):211-218. https://doi.org/10.1016/j.ijpharm.2012.01.030
- Levy MY, Benita S. Drug release from submicronized o/w emulsion: a new in vitro kinetic evaluation model. Int J Pharm. 1990;66(1-3):29-37. https://doi.org/10.1016/0378-5173(90)90381-D
- Dimartino G. Simultaneous determination of cholecalciferol (vitamin D3) and ergocalciferol (vitamin D2) in foods by selected reaction monitoring. J AOAC Int. 2009;92(2):511-517. https://doi.org/10.1093/jaoac/92.2.511
- Shah I, Petroczi A, Naughton DP. Method for simultaneous analysis of eight analogues of vitamin D using liquid chromatography tandem mass spectrometry. Chem Cent J. 2012;6:112. https://doi.org/10.1186/1752-153X-6-112
- Lipkie TE, Janasch A, Cooper BR, Hohman EE, Weaver CM, Ferruzzi MG. Quantification of vitamin D and 25- hydroxyvitamin D in soft tissues by liquid chromatography-tandem mass spectrometry. J Chromatogr B Anal Technol Biomed Life Sci. 2013;932:6-11. https://doi.org/10.1016/j.jchromb.2013.05.029
- Mattila PH, Piironen VI, Koivistoinen PE, Uusi-Rauva EJ. Contents of cholecalciferol, ergocalciferol, and their 25- hydroxylated metabolites in milk products and raw meat and liver as determined by HPLC. J Agric Food Chem. 1995;43(9):2394-2399. https://doi.org/10.1021/jf00057a015
- Mattila PH, Piironen VI, Uusi-Rauva EJ, Koivistoinen PE. New analytical aspects of vitamin D in foods. Food Chem. 1996;57(1):95-99. https://doi.org/10.1016/0308-8146(96)00144-6
- Mattila PH, Piironen VI, Uusi-Rauva EJ, Koivistoinen PE. Vitamin D contents in edible mushrooms. J Agric Food Chem. 1994;42:2449-2453. https://doi.org/10.1021/jf00047a016
- Mattila PH, Piironen VI, Uusi-Rauva EJ, Koivistoinen PE. Cholecalciferol and 25-hydroxycholecalciferol contents in fish and fish products. J Food Compos Anal. 1995 (8). 232-243. https://doi.org/10.1006/jfca.1995.1017
Yıl 2023,
Cilt: 27 Sayı: 3, 1213 - 1219, 28.06.2025
Öz
Kaynakça
- Tripkovic L, Lambert H, Hart K, Smith CP, Bucca G, Penson S, Chope G, Elina H, Berry J, Vieth R, Lanham-New S. Comparison of vitamin D2 and vitamin D3 supplementation in raising serum 25-hydroxyvitamin D status: a systematic review and meta-analysis. Am J Clin Nutr. 2012;95(6):1357-1364. https://doi.org/10.3945/ajcn.111.031070
- Sirajudeen S, Shah I, Al Menhali A. A narrative role of vitamin D and its receptor: With current evidence on the gastric tissues. Int J Mol Sci. 2019;20(15):3832. https://doi.org/10.3390/ijms20153832
- Piotrowska A, Wierzbicka J, Zmijewski MA. Vitamin D in the skin physiology and pathology. Acta Biochim Pol. 2016;63(1):17-29.https://doi.org/10.18388/abp.2015_1104
- Bocheva G, Slominski RM, Slominski AT. The impact of vitamin d on skin aging. Int J Mol Sci. 2021;22(16):9097. https://doi.org/10.3390/ijms22169097
- Bi Y, Xia H, Li L, Lee RJ, Xie J, Liu Z, Qiu Z, Teng L. Liposomal vitamin D3 as an anti-aging agent for the skin. Pharmaceutics. 2019;11(7):311. https://doi.org/10.3390/pharmaceutics11070311
- Singh Y, Meher JG, Raval K, Khan FA, Chaurasia M, Jain NK, Chourasia MK. Nanoemulsion: Concepts, development and applications in drug delivery. J Control Release. 2017;252:28-49. https://doi.org/10.1016/j.jconrel.2017.03.008
- Devireddy SK, Jonnalagadda LP. A literature review on self nanoemulsifying drug delivery system (SNEDDS). Int J Pharm Sci Rev Res. 2021;70(1):85-94. https://doi.org/10.47583/ijpsrr.2021.v70i01.011
- Cholecalciferol. National Library of Medicine. https://pubchem.ncbi.nlm.nih.gov/compound/Cholecalciferol. (accessed July 4, 2022)
- Ponto T, Latter G, Luna G, Leite-Silva VR, Wright A, Benson HAE. Novel self-nano-emulsifying drug delivery systems containing astaxanthin for topical skin delivery. Pharmaceutics. 2021;13(5):649. https://doi.org/10.3390/pharmaceutics13050649
- Ravisankar P, Naga Navya C, Pravallika D, Sri DN. A review on step-by-step analytical method validation. IOSR J Pharm. 2015;5(10):2250-3013.
- European Medicines Agency. ICH Topic Q 2 (R1) Validation of Analytical Procedures: Text and Methodology. London: European Medicines Agency; 2006.
- Miller JN, Miller JC. Statistics and chemometrics for analytical chemistry. Sixth. London: Pearson Education Limited; 2010. 119-127.
- AOAC International. Appendix F: Guidelines for Standard Method Performance Requirements. AOAC Int Off Method Anal. 2016;1-18.
- Williams RO, Sykora MA, Mahaguna V. Method to recover a lipophilic drug from hydroxypropyl methylcellulose matrix tablets. AAPS PharmSciTech. 2001;2(2):E8. https://doi.org/10.1208/pt020208
- Knoke S, Bunjes H. Transfer of lipophilic drugs from nanoemulsions into lipid-containing alginate microspheres. Pharmaceutics. 2021;13(2):173. https://doi.org/10.3390/pharmaceutics13020173
- Xu X, Khan MA, Burgess DJ. A two-stage reverse dialysis in vitro dissolution testing method for passive targeted liposomes. Int J Pharm. 2012;426(1-2):211-218. https://doi.org/10.1016/j.ijpharm.2012.01.030
- Levy MY, Benita S. Drug release from submicronized o/w emulsion: a new in vitro kinetic evaluation model. Int J Pharm. 1990;66(1-3):29-37. https://doi.org/10.1016/0378-5173(90)90381-D
- Dimartino G. Simultaneous determination of cholecalciferol (vitamin D3) and ergocalciferol (vitamin D2) in foods by selected reaction monitoring. J AOAC Int. 2009;92(2):511-517. https://doi.org/10.1093/jaoac/92.2.511
- Shah I, Petroczi A, Naughton DP. Method for simultaneous analysis of eight analogues of vitamin D using liquid chromatography tandem mass spectrometry. Chem Cent J. 2012;6:112. https://doi.org/10.1186/1752-153X-6-112
- Lipkie TE, Janasch A, Cooper BR, Hohman EE, Weaver CM, Ferruzzi MG. Quantification of vitamin D and 25- hydroxyvitamin D in soft tissues by liquid chromatography-tandem mass spectrometry. J Chromatogr B Anal Technol Biomed Life Sci. 2013;932:6-11. https://doi.org/10.1016/j.jchromb.2013.05.029
- Mattila PH, Piironen VI, Koivistoinen PE, Uusi-Rauva EJ. Contents of cholecalciferol, ergocalciferol, and their 25- hydroxylated metabolites in milk products and raw meat and liver as determined by HPLC. J Agric Food Chem. 1995;43(9):2394-2399. https://doi.org/10.1021/jf00057a015
- Mattila PH, Piironen VI, Uusi-Rauva EJ, Koivistoinen PE. New analytical aspects of vitamin D in foods. Food Chem. 1996;57(1):95-99. https://doi.org/10.1016/0308-8146(96)00144-6
- Mattila PH, Piironen VI, Uusi-Rauva EJ, Koivistoinen PE. Vitamin D contents in edible mushrooms. J Agric Food Chem. 1994;42:2449-2453. https://doi.org/10.1021/jf00047a016
- Mattila PH, Piironen VI, Uusi-Rauva EJ, Koivistoinen PE. Cholecalciferol and 25-hydroxycholecalciferol contents in fish and fish products. J Food Compos Anal. 1995 (8). 232-243. https://doi.org/10.1006/jfca.1995.1017
Toplam 24 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Farmasotik Kimya |
| Bölüm | Articles |
| Yazarlar | |
| Yayımlanma Tarihi | 28 Haziran 2025 |
| Yayımlandığı Sayı | Yıl 2023 Cilt: 27 Sayı: 3 |